seminario biología molecular
Post on 27-Jul-2015
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Is resistant to penicillin, and this has become a major challenge for the medical community.
Staphylococcus aureus can kill heart failure due to endocarditis
STAPHYLOCOCCUS AUREUS
MICROBIOTA
It can be defined as microorganisms that are often found in various parts of the body, associated with normal tissues skin and mucosa of the human body in healthy individuals
These help in the digestion of food, produce vitamins and protect against colonization by other microorganisms which may be pathogenic
ELECTROPHORESIS
Is called electrophoresis technique whereby the biomolecules in solution are separated when they are subjected to an electric field.
The separation is given by the electric charge and the molecular weight
GENERAL OBJECTIVE
The aim of this work was to optimize the study of Staphylococcus species diversity in human breast milk and neonate stool.
FUNDAMENTO DE LA EXTRACCION DEL DNA
EXTRACCION DEL ADN
1. Lisis celular
2. Precipitación de proteínas
3. Precipitación de DNA
4. Almacenamiento
Precipitación del RNA (RNAsa)
MATERIALES Y METODOSPCR
FUNDAMENTO PCR
1. Desnaturalización del DNA para dar hebras sencillas
2. Hibridación especifica de la hebra sencilla con un oligonucleotido
3. Replicación de la hebra sencilla por una DNA polimerasa a partir del olignonucleotido anterior como cebador
Se realizo esto para amplificar el gen tuf, se hizo por medio de 3 pares de primers
MATERIALES Y METODOSELECTROFORESIS
Es una técnica mediante la cual se separan las biomoléculas en disolución cuando se ven sometidas a un campo
eléctrico.
Electroforesis en gel de gradiente de temperatura utiliza ya sea una
temperatura o gradiente químico para desnaturalizar la muestra
cuando se mueve a través de un gel para separar acidos nucleicos
Los RFLP son marcadores genéticos del ADN y se pueden encontrar en regiones que codifican proteínas o exones, en los intrones o en el ADN que separa un gen de otro.
MATERIALES Y METODOSFRLP
La técnica RFLP se usa como marcador para identificar grupos particulares de personas con riesgo a contraer ciertas
enfermedades genéticas, en ciencia forense, en pruebas de paternidad.
Ejemplo
•De las 18 solo se discriminaron 15 bandas.
Se discriminaron con RFLP:
•S. Haemolyticus (182 y 139pb) - S. Scheiferi (no) (Bmpl)
•S. Cohnni (160 y 161pb) – S. Capitis (no) (Folk)
•S. Caprae (140, 114, 53,13pb) – S. Lugdunensis (140 y 180pb) (Acul)
AUTHOR WHAT HE SAID YES/NO
Ghebremedhin, B 16S rRNA gene is insufficient to identify Staphylococcus species and provides identification only to the genus level or for a group of species in the genus.
YES
Dahllöf, Drancourt and Raoult
The rpoB gene, proposed for community analyses, has classically been used for the identification of staphylococci because it is more discriminative then the 16S rRNA gene among staphylococci.
NO
1. This research showed that if all the hospitals in the world make this exam to all of the neonates, we could prevent the propagation of the agent.
2. The tuf-PCR TTGE exam is very effective and faster than the culture, which facilitates the attention in the hospitals.
3. However culture its very important because it allowed us to iddentificate other species that the PCR did not iddentificate.
4. The diagnosis is very important because it could save the baby’s life and make him not to suffer the consequenses of having the agent inside of him.
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