valentina hällefors physics sbcc ucsb detecon of ... hallefor… · of forensic significance...

Detec%onofSubstancesofForensicSignificanceUsingMicrofluidicsand

SERSFunding

Rustin Mirsafavi BMSE

Carl Meinhart Mechanical Engineering

Valentina Hällefors Physics SBCC UCSB

Real world applications Homeland security, medicine, drug trafficking

Our Research

The devices Inexpensive and reproducible

Real world applications Homeland security, medicine, drug trafficking

Our Research

The devices Inexpensive and reproducible

Microfluidics Control small amounts of fluid in a predictable way

Surface-enhanced Raman spectroscopy (SERS) Analyze composition, small sample volume

Real world applications Homeland security, medicine, drug trafficking

Our Research

This Summer:

•  Relate Raman spectra to concentration

Knownconcentra%on

This Summer:

•  Relate Raman spectra to concentration

Knownconcentra%on

•  Get Raman spectra for different concentrations

This Summer

•  Relate Raman spectra to concentration

Knownconcentra%on

•  Get Raman spectra for different concentrations

•  Build devices

Data Collection

1

2

3

Sample preparation

Data Collection

1

2

3

Sample preparation

•  0.1-100 µM sample •  Flow salt (LiCl) •  20 nm AgNPs

Data Collection

1

2

3

Microscope & fluidic processes

Data Collection

•  Low velocity creates separated flow

•  Mixture of fluids purely driven by diffusion

•  Salt induces aggregation of silver necessary for strong signal

Fentanyl

Data Collection

1

2

3 Laser and SERS

Data Collection

5 min

•  Map channel section with 633 nm laser

•  Run device 4-5 times

•  Stop after 10-12 minutes 10 min

Data – Noscapine/Papaverine

(100µM)

(100µM)

Data – References

Heroin

6-Monoacetylmorphine

Progress to date

Lab work Microfluidic devices, Raman microscope

Papaverine and noscapine Gathered data for 100uM

Reference spectra Heroin, morphine, 6-MAM

!  Test broader range of concentrations

!  Obtain data for other substances

!  Spectra-concentration relationship

Future work

Acknowledgments

Thank You!