An Introduction to Ultraviolet/visible Molecular

Absorption Spectrometry

1 Measurement of transmittance and absorbance

2 Beer’s law 3 The effects of instrumental noise on spectrop

hotometric analysis 4 Instrumentation

1 Measurement of Transmittance and Absorbance

2 Beer’s law

2-1 application of beer’s law to mixtrures 2-2 limitation to beer’s law

3 The Effects of Instrumental Noise on spectrophotometric Analysis

3-1 instrumental noise as a function of transmittance

3-2 sources of instrumental noise 3-3 effect of slit width on absorbance

measurements 3-4 effect of scattered radiation at wavelength

extremes of an instrument

4 Instrumentation

4-1 instrument components 4-2 types of instruments 4-3 some typical instruments

Applications of Ultraviolet/visible Molecular Absorption

Spectrometry

1 the magnitude of molar absorptivities 2 absorbing species 3 application of absorption measurement to qu

alitative analysis 4 quantitative analysis by absorption measure

ments 5 Photometric titrations 6 Photoacoustic spectroscopy

1 the magnitude of molar absorptivities

2 Absorbing Species

2-1 absorbing species containing π,σ,and n electrons

2-2 absorption involving d and f electrons

2-3 charge-transfer absorption

3 application of absorption measurement to qualitative analysis

3-1 methods of plotting spectral data

3-2 solvents

3-3 detection of functional groups

4 Quantitative Analysis by Absorption Measurements

4-1 scope

4-2 procedural details

4-3 derivative and dual-wavelength spectrophotometr

5 Photometric titrations

5-1 titration curve

5-2 instrumentation

5-3 application of photometric titrations

6 Photoacoustic Spectroscopy

6-1 the photoacoustic effect 6-2 photoacoustic spectra 6-3 instruments 6-4 applications