An Introduction to Ultraviolet/visible Molecular
Absorption Spectrometry
1 Measurement of transmittance and absorbance
2 Beer’s law 3 The effects of instrumental noise on spectrop
hotometric analysis 4 Instrumentation
1 Measurement of Transmittance and Absorbance
2 Beer’s law
2-1 application of beer’s law to mixtrures 2-2 limitation to beer’s law
3 The Effects of Instrumental Noise on spectrophotometric Analysis
3-1 instrumental noise as a function of transmittance
3-2 sources of instrumental noise 3-3 effect of slit width on absorbance
measurements 3-4 effect of scattered radiation at wavelength
extremes of an instrument
4 Instrumentation
4-1 instrument components 4-2 types of instruments 4-3 some typical instruments
Applications of Ultraviolet/visible Molecular Absorption
Spectrometry
1 the magnitude of molar absorptivities 2 absorbing species 3 application of absorption measurement to qu
alitative analysis 4 quantitative analysis by absorption measure
ments 5 Photometric titrations 6 Photoacoustic spectroscopy
1 the magnitude of molar absorptivities
2 Absorbing Species
2-1 absorbing species containing π,σ,and n electrons
2-2 absorption involving d and f electrons
2-3 charge-transfer absorption
3 application of absorption measurement to qualitative analysis
3-1 methods of plotting spectral data
3-2 solvents
3-3 detection of functional groups
4 Quantitative Analysis by Absorption Measurements
4-1 scope
4-2 procedural details
4-3 derivative and dual-wavelength spectrophotometr
5 Photometric titrations
5-1 titration curve
5-2 instrumentation
5-3 application of photometric titrations
6 Photoacoustic Spectroscopy
6-1 the photoacoustic effect 6-2 photoacoustic spectra 6-3 instruments 6-4 applications