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    PJAS December 2010

    The Effect of Detergent

    Concentration on Plant

    GrowthErin AnnunziatoMs. Pietrangelo

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    Reason- Environment

    laundry detergent in run-off

    fatal to plants

    human obstinacy

    more eco-friendly run-off

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    Hypothesis

    If there is more than 5% laundrydetergent in the water given to the

    plants, then the plants will notgrow.

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    Types of Peas

    Field Peas:

    split peas

    used in farming

    Pisum arvense

    Gardening Peas:

    popular for home gardeners

    sweeter and softer

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    Growing Pea Plants

    Environment:

    about 12-24 C (55-75 F)

    moist soil

    sunny or partially-sunny

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    Key Terms

    photosynthesis: energy from the sunlight

    water and carbon dioxide -> food and oxygen

    detergent: cleans soiled surface (usually refers only to synthetic detergents)

    soap- a kind of detergent (different chemical-makeup than syntheticdetergents)

    surfactants/surface active agents- consists of molecules that pull away dirtparticles from surface

    ANOVA- an analysis of variance

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    Lab Details

    Independent Variable: concentration of detergent in thewaterLevels: 1% detergent, 2% detergent, 3% detergent,4%detergent, 5% detergent, 6% detergent, and 7%detergentTrials: 3Control: 0% detergentDependent Variable:Growth of plants (cm)Constants: type of plant, total amount of

    detergent/water added,, watering times and frequency, growingenvironment and time

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    Design Diagram

    Levels

    detergent

    (Control)

    0%

    1% 2% 3% 4% 5% 6% 7%

    Results of

    Trials

    1-3

    Average

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    Materials

    Pisum sativumseeds

    Tide Laundry Detergent

    water

    potting soil

    8 pots- 11 centimeter circumference; 9.5 centimeter height

    graduated cylinder

    ruler

    7 plastic bottles

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    Procedure

    1. Plant 5 pea seeds 2 centimeters deep per 8 pots, each pot filled 6.3 centimeters with potting soil

    2. Water the pots with pure water (0% detergent) every day until stems are at adequate heights to begin experiment

    3. Measure test levels 1-7% detergent by mixing water and detergent in 500 mL water plastic bottles:

    1%: 5 mL of laundry detergent and 495 mL of water

    2%: 10 mL of laundry detergent and 490 mL of water

    3%: 15 mL of detergent and 485 mL of water

    4%: 20 mL of detergent and 480 mL of water

    5%: 25 mL of detergent and 475 mL of water

    6%: 30 mL of detergent and 475 mL of water

    7%: 35 mL of detergent and 470 mL of water

    4. Water plants each pot with the same amount (100 mL) of corresponding concentration level every day.

    5. Record the plant height in centimeters every third day.

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    Nov. 13 Nov. 16 Nov. 19 Nov. 22

    0% 12.3 15 16.6 16.9

    1% 12.6 14

    2% 5.5 5.5

    3% 12.8 134% 10.6 11.8

    5% 10.5 10.75

    6% 13 13.6 12.25

    7% 9.3 9.2

    Average Plant Heights (cm)

    Results

    Measurement Day

    He

    ights(cm)

    * These values have been gathered with only two trials, rather than three

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    Average Growth (cm)

    Results

    Measurement Day

    Differencein

    He

    ights(cm)

    start-13 13-16 16-19 19-22

    0% 0 2.7 1.6 0.3

    1% 0 1.4

    2% 0 0

    3% 0 0.2

    4% 0 1.2

    5% 0 0.7

    6% 0 0.6 -1.35

    7% 0 -0.1

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    Addressing 6%

    height of plant 1 decreased

    cells may have shrunk

    unlikely measurement error because there is a trend

    plant 1 was the tallest

    plant 3 increased, then decreased

    plant 2 did not change then died

    13th 16th 19th 22nd

    1 17 16.5 15

    2 14 14

    3 8 10.5 9.5

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    ANOVA: Results

    Source of Sum of d.f. Mean F

    Variation Squares Squares

    between 202.8 7 28.97 1.742error 266.2 16 16.64total 469.0 23

    The probability of this result, assuming the null

    hypothesis, is 0.169The null hypothesis is not rejected:

    There was no significant difference of the heights between the different

    groups that were treated with different concentrations of detergent

    Null hypothesis: There are no differences in the

    observed heights between the treatment groups

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    Conclusions

    Hypothesis:

    If there is more than 5% laundry detergent in the water given to theplants, then the plants will not grow.

    I cannot support nor refute my hypothesis

    1-5% died, while 6% survived

    not enough data

    control survived

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    Sources of Error

    inadequate sunlight

    different starting maturity

    small pots

    human error

    graduated cylinder

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    Improvements

    more mature plants

    growing lamps

    same plant height

    lower laundry detergent concentrations

    larger growing space

    regulate root depth

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    Extensions

    root depth

    soap

    different plants

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    Thank you :)

    Ms. Pietrangelo

    My mother and my au pair