analysis of sugars in honey and syrup - nacalai.co.jp · technical ote analysis of sugars in honey...
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Technical Note
Analysis of Sugars in Honey and Syrup
Analysis of honey and syrup is an important part of detecting food adulteration. Sugar-D provides good and consistent separation with high durability.
Standards Honey
COSMOSIL Application Data
NACALAI TESQUE, INC
Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI
Sample: 1; Fructose (30mg/ml)2; Glucose (24mg/ml)3; Sucrose (6mg/ml)4; Maltose (6mg/ml)
Inj.Vol.: 5.0µl
COSMOSIL Application Data
NACALAI TESQUE, INC
Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI
Sample: Honey (50mg/ml)1; Fructose2; Glucose
Inj.Vol.: 5.0µl
Gum syrup Gum syrup (contains honey)
COSMOSIL Application Data
NACALAI TESQUE, INC
Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI
Sample: Gum syrup (50mg/ml)1; Fructose2; Glucose
Inj.Vol.: 5.0µl
COSMOSIL Application Data
NACALAI TESQUE, INC
Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI
Sample: Gum syrup (contains honey)(50mg/ml)1; Fructose2; Glucose
Inj.Vol.: 5.0µl
Maple syrup Sugar syrup (Maple flavor)
COSMOSIL Application Data
NACALAI TESQUE, INC
Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI
Sample: Maple syrup (50mg/ml)1; Fructose2; Glucose 3; Sucrose
Inj.Vol.: 5.0µl
COSMOSIL Application Data
NACALAI TESQUE, INC
Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI
Sample: Sugar syrup (Maple flavor)(50mg/ml)1; Fructose2; Glucose 3; Sucrose
Inj.Vol.: 5.0µl
1701
For research use only, not intended for diagnostic or drug use.
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Apple syrup Agave syrup
COSMOSIL Application Data
NACALAI TESQUE, INC
Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI
Sample: Apple syrup (50mg/ml)1; Fructose2; Glucose
Inj.Vol.: 5.0µl
COSMOSIL Application Data
NACALAI TESQUE, INC
Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI
Sample: Agave syrup (50mg/ml)1; Fructose2; Glucose
Inj.Vol.: 5.0µl
Starch syrup Starch syrup (fast analysis)
COSMOSIL Application Data
NACALAI TESQUE, INC
Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI
Sample: Starch syrup (50mg/ml)1; Glucose2; Maltose3; Maltotriose
Inj.Vol.: 5.0µl
COSMOSIL Application Data
NACALAI TESQUE, INC
Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 60/40Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI
Sample: Starch syrup (50mg/ml)1; Glucose2; Maltose3; Maltotriose
Inj.Vol.: 5.0µl
More Sugar-D application data are available on our website (http://www.nacalai.co.jp/ cosmosil/data/csmosrchtop.cfm). Or you can search “COSMOSIL Application”.
DurabilityThe decrease of retention time was compared between COSMOSIL Sugar-D and conventional aminopropyl bonded stationaryphase under severe condition of 100% water as eluent between tests. The capacity factor did not decrease in case to COSMOSIL Sugar-D.
[Test Condition]Column SizeMobile PhaseFlow RateTemperatureDetectionSample
4.6 mm I.D. x 250 mmAcetonitrile : Water = 70 : 301.0 ml/min30RIMaltose
[Eluent Condition]EluentFlow RateTemperature
Water1.0 ml/minRT
120%
0%
100%
80%
60%
40%
20%
0 12010080604020Time (hr)
Sugar-DAminopropyl Type