anti-erβ2 clone 57/3 product name anti human erβ2 clone 57/3 · 2018-08-20 · zannoni g f,...

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DatasheetAnti-ERβ2Clone57/3ProductName AntiHumanERβ2Clone57/3

CatalogueNumber 57/3

Clone,Isotype 57/3,IgG1

Format IgG

TestedApplications IHC-P,WB,IF

Description:Estrogenreceptorsarecommonlyover-expressedincancers.Clone57/3recognizesEstrogenReceptorBeta(ERβ)2andisusefulindeterminingtheexpressionofERβ2incancercellsviavariousanalysismethods.ProductDetails:Forminstock:IgG,purified–1.0mg/mL.Alsoavailableasunpurifiedsupernatant.Host:MouseSpecificity:RecognizesERβ2isomeratsequenceCGMKMETLLPEATMEQ.HumanHistologypositivecontrol:TestisFusionpartner:SpleencellsfromimmunisedBalb/cmicewerefusedwithcellsoftheSP2/0myelomacellline.Storage:Storeat+4°Cor-20°C.Avoidrepeatedfreezingandthawing.Shelflife:18monthsfromdateofdispatch.Regulatory/Restrictions:Forresearchandcommercialpurposes.Applications SuggestedDilutionImmunohistochemistry–Paraffin 1:50-1:500WesternBlot 1:5003,4,8Immunofluorescence 1:1005

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Applications:Clone57/3usedtodetectexpressionofERβ2incancertissuebyIHC-PImagecaption:ImmunoexpressionofERsinendometrialcancers.Tissueswereclassifiedaswell(A-D),moderately(E-H)orpoorly(J-M)differentiated;...immunoexpressionofERβ1,2,5wasreadilydetected(K.L,M).InsertsinpanelsK,L,andMshownegativecontrolsforERβ1,ERβ2andERβ5antibodiesrespectivelygeneratedusingprimaryantibodiespre-absorbedwithspecificpeptidesusedforimmunisation.Asterisks(*)labelthestromalcompartmentthatwaswelldefinedinthewelldifferentiatedcancers.(Collins,Fetal.)Dilutionused:1:30

Clone57/3usedtodetectexpressionofERβ2incancertissuebyIHC-PImagecaption:A)ImmunostainingforERβ1wassignificantlyhigherintumorsfromfemalescomparedtomales(**p<0.01);B)andC)TumorlevelsofERβ2andERβ5didnotsignificantlydifferbetweenfemalesandmales.Allresultsareexpressedasthemean±SEM(n = 6tumors/group).Representativestainedsectionoftumorsfromfemalesandmalesareshown(magnification40x).IHC,immunohistochemicalscore.(Ciucci,Aetal.)Dilutionused:1:100

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Clone57/3usedtodetectERβ2expressioninnormalhumanliverbyIHC-PImagecaption:StainingpatternoftheERßisoformsinhumanliver.InordertoconfirmthespecificityoftheERßantibodiesusedinthestudy,weimmunostainedsectionsofhumanhealthyliver,showingthatERß1andERß2isoformsarenotexpressed,whilespecificnuclearERß5immunoreactivityisdetected(magnification20xand40x).ThispatternofERßisoformsimmunoreactivityisconsistentwithpreviousdescriptions.(Ciucci,Aetal.)Dilutionused:1:100

Clone57/3usedtodetectexpressionofERβ2incolorectaltissuebyIHC-PImagecaption:ExamplesofERβ1(a,d,e),ERβ2(b,f,g),ERβ5(c,h)andTFF1(i)immunohistochemistryperformedonnormalcolorectalepithelium(a–c)andcolorectalcarcinoma(d–i)...(Wong,N.Aetal.)Dilutionused:1:50

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Clone57/3usedtodetectexpressionofERβ2incolorectaltissuebyWesternBlotImagecaption:(A)ERβ1,2and5mRNAexpression(assessedbyRT-PCR)and(B)ERβ1and2proteinexpression(assessedbywesternblotting)by10colorectalcarcinomacelllines...(Wong,N.Aetal.)Dilutionused:1:500

Clone57/3usedtodetectexpressionofERβ2inLeydigcelltumorsbyWesternBlotImagecaption:ImmunoblotsofaromataseandestrogenreceptorsfromproteinextractsofLeydigcelltumorsandcontroltissues.P450arom:ratovary(C+),negativecontrol(C−),Leydigcelltumors(lanes1and2).ERα:ratovary(C+),negativecontrol(C−),Leydigcelltumors(lanes1and2).ERβ1:controlhumantestis(C+),negativecontrol(C−),Leydigcelltumors(lanes1and2).ERβ2:controlhumantestis(C+),negativecontrol(C−),Leydigcelltumors(lanes1and2).β-Actinservesasaloadingcontrol.(Carpino,Aetal.)Dilutionused:1:500

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Clone57/3usedtodetectexpressionofERβ2inLeydigcelltumorsbyIHC-PImagecaption:MorphologyandimmunolocalizationofaromataseandestrogenreceptorsinLeydigcelltumors(AandB)andcontroltestis(C).A–C:Hematoxylin–eosinstaining.A1–C1:Aromataseimmunolocalization.A2–C2:ERαimmunoreactivity.A3–C3:ERβ1immunodetection.A4–C4:ERβ2immunolocalization.L,Leydigcells;T,seminiferoustubule.(Carpino,Aetal.)Dilutionused:1:100

Clone57/3usedtodetectexpressionofERβ2inovariancancercellsbyImmunofluorescenceImagecaption:A)RepresentativepicturesforERα,ERβ1,ERβ2,ERβ5,PR,andARimmunostaininginLGSOCpatients(magnification20×)andbarchartshowinglevelsofhormonereceptorexpression(mean±SEM,n=25).ResultsareexpressedasIRS(Immunoreactivereceptorscore).B)Representativepicturesshowingexclusivenuclearandnuclear/cytoplasmicERβ2expressioninprimarytumorandmatchedperitonealmetastasis.(Buttarelli,Metal.)Dilutionused:1:100

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References:

1. Collins,F.,MacPherson,S.,Brown,P.,Bombail,V.,Williams,A.R.,Anderson,R.A.,Saunders,P.T.(2009).Expressionofoestrogenreceptors,ERα,ERβ,andERβvariants,inendometrialcancersandevidencethatprostaglandinFmayplayaroleinregulatingexpressionofERα.BMCCancer,9,330.

2. Ciucci,A.,Meco,D.,DeStefano,I.,Travaglia,D.,Zannoni,G.F.,Scambia,G.,Gallo,D.(2014).GenderEffectinExperimentalModelsofHumanMedulloblastoma:DoestheEstrogenReceptorβSignalingPlayaRole?PLoSONE,9(7),e101623.

3. Wong,N.A.,Malcomson,R.D.,Jodrell,D.I.,Groome,N.P.,Harrison,D.J.andSaunders,P.T.(2005).ERβisoformexpressionincolorectalcarcinoma:aninvivoandinvitrostudyofclinicopathologicalandmolecularcorrelates.J.Pathol.,207:53–60.

4. Carpino,A.,Rago,V.,Pezzi,V.,C.(2007)DetectionofAromataseandEstrogenReceptors(ERalpha,ERbeta1,ERbeta2)inHumanLeydigCellTumor.EuropeanJournalofEndocrinology;157(2):239-44.

5. Buttarelli,M.,Mascilini,F.,Zannoni,G.,Ciucci,A.,Martinelli,E.,Filippetti,F.,Scambia,G.,Ferrandina,G.,Gallo,D.(2017)Hormonereceptorexpressionprofileoflow-gradeserousovariancancers.GynecologicOncology,Volume145,Issue2,Pages352-360,ISSN0090-8258.

6. Rago,V.,Romeo,F.,Giordano,F.,Ferraro,A.,Andò,S.,&Carpino,A.(2009).IdentificationofERβ1andERβ2inhumanseminoma,inembryonalcarcinomaandintheiradjacentintratubulargermcellneoplasia.ReproductiveBiologyandEndocrinology :RB&E,7,56.IHC-P,Dilutionused1:100

7. Skliris,G.P.,Leygue,E.,Curtis-Snell,L.,Watson,P.H.,&Murphy,L.C.(2006).Expressionofoestrogenreceptor-βinoestrogenreceptor-αnegativehumanbreasttumours.BritishJournalofCancer,95(5),616–626.IHC-P,Dilutionused1:20

8. Ogawa,S.,Inoue,S.,Watanabe,T.,Orimo,A.,Hosoi,T.,Ouchi,Y.,&Muramatsu,M.(1998).Molecularcloningandcharacterizationofhumanestrogenreceptorbetacx:apotentialinhibitorofestrogenactioninhuman.NucleicAcidsResearch,26(15),3505–3512.WB,Dilutionused1:500

9. Critchley,H.,Henderson,T.,Kelly,R.,Scobie,G.,Evans,L.,Groome,N.P.,Saunders,P.T.K.(2002)Wild-TypeEstrogenReceptor(ERβ1)andtheSpliceVariant(ERβcx/β2)AreBothExpressedwithintheHumanEndometriumthroughouttheNormalMenstrualCycle.JournalofClinicalEndocrinology&Metabolism;87(11):5265-5273.IHC-P,Dilutionused1:50

10. ZannoniGF,CiucciA,MarucciG,TravagliaD,StiglianoE,FoschiniMP,ScambiaG&GalloD(2016)Histopathology68,541–548.IHC-P,Dilutionused1:100

anti-erβ2 clone 57/3 product name anti human erβ2 clone 57/3 · 2018-08-20 · zannoni g f,...

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