app 24 item 9 evaluation report for nrl fmd laboratories ... · 39-th general session of eu fmd...
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EVALUATION REPORTEVALUATION REPORT
for NRL FMD laboratories of Balkan countries
39-th General Session of EU FMD Commission, 27-27 April, 2011, Roma, Italy
Balkan countries
Prof. Dr. Georgi Georgiev,DVSci
BULGARIA
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The aim:
To summarize the progress of FMDNRLs of the Balkanic countries in theirdiagnostic capacity
(scope and range of analytical
39-th General Session of EU FMD Commission, 27-27 April, 2011, Roma, Italy
(scope and range of analyticaldiagnostic methods, needs fordiagnostic tools, training, knowledgeand experience, accreditation level andparticipation in ring tests)
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The goals is :
1. To evaluate the diagnostic capacity and identify the needs for training and other forms of support
2. To find the gaps and weaknesses of different countries/NRLs and submit the
39-th General Session of EU FMD Commission, 27-27 April, 2011, Roma, Italy
different countries/NRLs and submit the proposal to the EC for improving the situation with FMD surveillance in these countries through the EU FMD Commission mechanism
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The surveyed countries 2010 were:
• Albania – UN/FAO, EU FMD
• Serbia – UN/FAO, EU FMD, negotiated with EU
• Montenegro – UN/FAO
• Macedonia UN/FAO
• Bosnia & Herzegovina – UN/FAO
39-th General Session of EU FMD Commission, 27-27 April, 2011, Roma, Italy
• Bosnia & Herzegovina – UN/FAO
• Croatia - UN/FAO, negotiated with EU
• Bulgaria – UN/FAO, EU FMD, member of EU
• Romania – UN/FAO, EU FMD member of EU
• Kosovo – special status UN
• Moldova – UN/FAO
• Slovenia - UN/FAO, EU FMD, member of EU
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Session of Exc. Commity of EU FMD Comission, Vienna, 2010
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Session of Exc. Commity of EU FMD Comission, Vienna, 2010
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Session of Exc. Commity of EU FMD Comission, Vienna, 2010
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Session of Exc. Commity of EU FMD Comission, Vienna, 2010
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Session of Exc. Commity of EU FMD Comission, Vienna, 2010
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Session of Exc. Commity of EU FMD Comission, Vienna, 2010
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Session of Exc. Commity of EU FMD Comission, Vienna, 2010
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Session of Exc. Commity of EU FMD Comission, Vienna, 2010
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Session of Exc. Commity of EU FMD Comission, Vienna, 2010
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Session of Exc. Commity of EU FMD Comission, Vienna, 2010
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Session of Exc. Commity of EU FMD Comission, Vienna, 2010
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First set of questions:
• Methods for FMD antigen and antibody detection used routinely in your NRL
• Development of Standard Operating Procedures (SOPs) and using standard (reference) materials
39-th General Session of EU FMD Commission, 27-27 April, 2011, Roma, Italy
(reference) materials
• Organization/participation of proficiency tests (PT)
• Developing accreditation strategyregarding the ISO 17025 standard, level of accreditation and EQ&A
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Second set of questions:
• Organization of workshops/training courses/ simulating exercises
• Ad hoc (real time) trainings delivered by the WRL/EU-RL or other institutions (i.e. trainings provided to your NRL/ONL in addition to / or organized in connection with the annual workshops)
39-th General Session of EU FMD Commission, 27-27 April, 2011, Roma, Italy
organized in connection with the annual workshops)• Other activities carried out by the FMD WRL/EU-RL
(not mentioned in this questionnaire) able to assist FMD NRL over the last 5 years
• Sharing the information and communication of NRLs with FMD WRL/EU-RL or other institutions
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Conclusions:
1. 10/11 of 11 FMD diagnostic laboratories are determined as FMD NRLs of countries in the region
2. 9 of 11 FMD NRLs declare that WRL in
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2. 9 of 11 FMD NRLs declare that WRL in Pirbright UK is their Reference laboratory
3. Only 4 of 11 can perform routinely FMD Ag ELISA
4. Only 4 of 11 can perform routinely FMDLPBL Ag ELISA
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Conclusions (cont.):
5. Only 4 of 11 can perform routinely FMD SPCE Ab ELISA
6. 10 of 11 can perform routinely FMD NSP ELISA tests
7. Only 5 of 11 can perform routinely
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7. Only 5 of 11 can perform routinely RT-PCR (real or conventional)
8. 1 of 11 can perform routinely FMD VI (but unfortunately up to 2010)
9. No one can perform routinely VNT
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Conclusions (cont.):
10. Most of the NRLs are used developed and validated Standard Operating Procedures (SOPs) for methods routinely performed at the
39-th General Session of EU FMD Commission, 27-27 April, 2011, Roma, Italy
methods routinely performed at the lab., but few of them used standard (reference) materials distributed by WRL,UK
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Conclusions (cont.):
11.There are remarkable improvement for attempt to participate in a different levels of PT organized by WRL/EU FMD RL, UK
11.1. Five countries (Alb, Kos, Mol, B&H and Mon) are not participated in PT organized
39-th General Session of EU FMD Commission, 27-27 April, 2011, Roma, Italy
Mon) are not participated in PT organized by WRL/EU FMD RL, Pirbright, UK
11.2. Other 6 countries (Cro, Ser, Mac, Rom, Slov, BG) in last 5 years try to cover different panel 1-4 levels of PT with inactivated or live virus materials
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Accreditation strategy
(ISO 17025 standard) and EQ&A:
12. Some of institutions that FMD NRLsbelongs has accreditation of methodsunder ISO 17025, but for FMDV – not!
The reason is small number of
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The reason is small number ofsamples received for testing
13. Only few FMD NRLs (BG, Rom, Ser)declared accreditation of methods forFMDV Ag and Ab detection covering theISO-17025 standard
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Recommendations:• All labs in the region should be able to
investigate clinical suspicions of FMD with a quick, reliable and sensitive test methods
• FMDV ”yes” or ”no” may be sufficient on a
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• FMDV ”yes” or ”no” may be sufficient on a country level
• (Real-time) RT-PCR diagnostics and/or LFD tests should be implemented
• Harmonisation of test protocols is very important!
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Recommendations:
• Type-specific diagnosis and further characterisation of strains through WRL and dedicated labs in the region should be agreed upon.
39-th General Session of EU FMD Commission, 27-27 April, 2011, Roma, Italy
agreed upon.
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Recommendations:
Because 12 of 13 countries in the regionare accepted non vaccination policy for
FMD control NS ELISA tests should be
used for serology survey investigations
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used for serology survey investigations
Further characterisation of some positiveswill be confirmed through WRL ordedicated labs in the region should beagreed upon.
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Recommendations:
• The tripartite mechansm under OIE, FAO, and EC for evaluation the control of FMD and other Exotic diseases (BT, S&G Pox, PPR, CSF) for Bulgaria, Greece and
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PPR, CSF) for Bulgaria, Greece and Turkey should be extended and finnaced under the GF TADS for Europe for other 9 countries in the Region (Alb, Mont, Ser, Cro, B&H, FYROM, Ro. Mol and Kos)
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Recommendations:
• Training of laboratory staff andenhancing capacity of relevant expertsfrom the veterinary authorities of thecountries from the region should be a
39-th General Session of EU FMD Commission, 27-27 April, 2011, Roma, Italy
countries from the region should be apriority of EU FMD commisiion
involving in this responsibility oneor more of its country memberstates
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Recommendations:
• Reenforcement of the competentauthorities to implement continuoussurveillance system for confirming thenegative status of each Balkannic
39-th General Session of EU FMD Commission, 27-27 April, 2011, Roma, Italy
negative status of each Balkanniccountry is esential!
• Enlarging the number of investigatedsamples will support the accreditationpolicy of the conrties for covering theISO 17025 standard methods.
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National Veterinary ServiceNational Veterinary Service
BULGARIABULGARIA
Thank you for your
39-th General Session of EU FMD Commission, 27-27 April, 2011,
Roma, Italy
Thank you for your attention!