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Supplementary Information
The Woronin body in the nematophagous fungus Arthrobotrys
oligospora is essential for trap formation and efficient pathogenesis
Lian-Ming Liang†, Hong Gao†, Jin-Zhou Li, Lu Liu, Zhi-Heng Liu & Ke-Qin
Zhang*
State Key laboratory for Conservation and Utilization of Bio-Resources in Yunnan,
Yunnan University, Kunming, Yunnan, China.
† These authors contributed equally to this work.
*Corresponding author. Tel: +86 871 65034878; Fax: +86 871 65034838, E-mail
address: [email protected].
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Supplementary figureSupplementary Fig. 1 The alignment of AoHex1 and Hex homologs from nine
other fungi. Amino acids in the C terminus are more conserved that those in the N
terminus. The peroxisome target signal sequences, S-R-L, exist in the C termini of
seven Hex proteins, while AoHex1 and a Hex homolog from D. haptotyla instead had
FKL and YKL in the corresponding positions which may also function as peroxisomal
targeting signals.
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Supplementary Fig. 2 A phylogenetic tree of AoHex1 homologs constructed by
neighbor-joining method. The GenBank accession numbers were indicated in the
brackets. * indicates the branch from nematode-trapping fungi.
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Supplementary Fig. 3 A neutral red staining of the hypha of the wild-type and
ΔAoHex1 strains. The two strains were cultured on PDA plates for 5 days. The hypha
of both strains were directly stained with neutral red staining solution (BBI Life
Sciences, Shanghai, China) for 10 minutes and rinsed twice with distilled water, 2
minutes each. The stained hypha were observed under a light microscope. A, the wild-
type strain. The cherry red spots indicate the vacuoles. B, the ΔAoHex1 strain.