Performance of microbial induced carbonate precipitation for immobilizing Cd

in water and soil

Dinghua Peng1, Bin Wu1, Suyu Qiao1, Hang Ma1, Lei Zhang1, Siyu Hou1, Bin Wu1

Heng Xu1*

1Key Laboratory of Bio-Resource and Eco-Evironment of Ministry of Education,

College of Life Sciences, Sichuan University, Chengdu 610065, P.R.China

Table captions for supplementary material

11* Corresponding author: xuheng64@sina.com (H.Xu). Tel: +86 28 85414644; Fax: +86 28 85418262

Table S1. Main properties of soil, OS and urea used in the experiment

Table S2. Urease activity between Cd-resistant bacteria strains

Table S3. Effect of different treatments on soil bacterial community richness and

diversity indices at 40 days after incubation

Figures captions for supplementary material

Figure S1. Color change of solid urease selective medium with un-inoculation (a) and

inoculation (b) Cd-resistant bacteria cell

Figure S2. The growth rate of bacterial cells

Figure S3. Images of precipitation induced by strain CJW-1 with 40 mM CaCl2 and

2% urea in 40 mg/L Cd contaminated medium.

Table S1 Main properties of soil, OS and urea used in the experimentProperty Soil OS Urea

Sand (%) 26.23 - -Silt (%) 22.35 - -Clay (%) 51.42 - -

pH 7.20 8.52 9.24Organic matter(g/kg) 41.00±0.53 12.60±0.22 -

Cation exchange capacitya (cmol/kg)

19.80±0.33 1.32±0.12 -

Total N (g/kg) 2.28±0.29 1.46±0.09 - Total P (mg/kg) 693±0.22 601±1.12 -Total K (g/kg) 17.70±0.31 2.00±0.30 -

Total Cd (mg/kg) 20.62±0.13 0.12±0.02 -Values are expressed as mean ± standard error (n=3).

Table S2 Urease activity between Cd-resistant bacteria strains

Isolated strain Urease activity

CJW-1 3.242CJW-2 0.021

Urease activity was represented by OD values in solution at day 5

Table S3 Effect of different treatments on soil bacterial community richness and

diversity indices at 40 days after incubation

Treated samples Ace a Chao 1a Shannon b Simpson b Coverage OTUs

CK 1492.79 1529.10 5.26 0.018 99.29% 1268

T1 1281.35 1280.10 4.83 0.021 99.20% 1003

T2 1463.18 1478.95 5.15 0.020 99.35% 1279

T3 1332.73 1309.26 4.58 0.052 99.27% 1116

T4 1379.96 1424.10 4.54 0.050 99.13% 1119

T5 906.81 862.22 1.65 0.599 99.21% 646

T6 1169.47 1167.17 3.59 0.159 99.15% 869

T7 1146.62 1174.41 4.15 0.078 99.28% 894

a Richness indices b diversity indices

Figure S1 Color change of solid urease selective medium with un-inoculation (a) and

inoculation (b) Cd-resistant bacteria cell

a

b

Figure S2 The growth rate of bacterial cells

Figure S3 Images of precipitation induced by strain CJW-1 with 40 mM CaCl2 and

Un-inoculation with Cd-resistant bacteria

Inoculation with strain CJW-2Inoculation with strain CJW-1

2% urea in 40 mg/L Cd contaminated medium.