Table A.1 46 pairs of SSR primers used in the study

Marker Primer sequence (5’ – 3’)Range of alleles size (bp)

Repeat pattern Chromosome

BSAT1.021 ATGCTTCAAGAGATCCGAAC 99-157 (TA)25 1TTATCATACATACGATGGCAATG

BSAT1.001 GGGCCGACTTATGCATTTTT 90-160 (TA)34 1GCTTTAGAGAGAGGGATCAATACA

BSAT1.024 CCATCTGTGTATCAGAATCGC 112-420 (AG)25 1ATGATCCCTCCTCATGCC

BSAT1.009 TTCCTAATAAAGTGCAAGACCC 212-255 (TA)25 1AAACTACCACCACAAATCCTTAAT

BSAT1.011 GATCTTGGAGAGAGTGATCGA 178-190 (AAG)10 1AAGACACATGAAATAGTAACGACG

BSAT1.014 TTGACTGAAGATAGGAATGAAGG 185-278 (ATT)22 1ATACGATTAGTTTAGCGGTGTATG

BSAT1.012 ATGGACTCACCTAATCGGAA 110-182 (AT)44 1AAATGAGAATATAGTTTGCTCCG

BSAT1.013 CCAAAAACAAAGAGGGTTAGC 138-150 (A)31 1GGAAGACAAAACACATAAGAAAGG

BSAT1.017 ACCCACTCTACTTCCCCATT 185-206 (A)27 1TTGAACCCGAAAAGAACATC

ATHACS AGAAGTTTAGACAGGTAC 259-262 (A)36 1AAATGTGCAATTGCCTTC

ATHGENEA ACCATGCATAGCTTAAACTTCTTG 205-221 (A)39 1ACATAACCACAAATAGGGGTGC

AtEAT1 TGCGTGAATGATATG-TAACTGG 162-172 (AT)11 1TTTGATTGCCTTATTTGTCTGC

ACC2 AAATGTGCAATTGCCTTC 209-287 (T)37 1AGAAGTTTAGACAGGTAC

RCVI5 ACATAACCACAAATAGGGGTGC 162-215 (T)33 1ACCATGCATAGCTTAAACTTCTTG

BSAT2.012 GTAATCTTATCTTCCTCCGACG 134-177 (TA)24 2GTTTCCCCTCGTTCTTTACG

BSAT2.014 AACATACAAACTCAACAAAATTGC 223-287 (AT)35 2GACAAGAAGAAGAAACTTGAAACC

BSAT2.007 CCTGTGTCTTTTACAGTTTCCA 190-215 (A)36 2TGCCCAACAACAGAATTACA

PLS7 GATGAATCTTCTCGTCCAAAAT 86-144 (AT)12(AG)8 2GACAAACTAAACAACATCCTTCTT

MSAT2.5 TGAGAGGGACAGATAGGAA 161-225 (CT)41 2ATCAAAAGGGATACTGACAA

T3F17 CTGAGTTTATACCGTTGCATCC 146-150 (AG) 15 2AAAAAGTGGAAAAACCCTACCC

NGA168 GAGCTTGTGCCAGTGTCG 135-151 (GA)25 2GGAGCCTCGAATGATGAAGC

BSAT3.001 TACACGGAATCAATCAAAAGG 94-171 (AAG)8 3TCTCACGAACATAGCCATCA

BSAT3.007 ACTCCGAATGCTTTATTTTCTG 153-203 (AT)26 3ATTCATACCGACCGAAGATG

BSAT3.012 TACTCAATGCTGTGTGATGATG 100-121 (AAG)11 3CGTTTCTGCTGGAAGGTATC

MSAT3.1 GGACTCGCTGCTGCTACATTC 181-480 (GA)28 3CCACGCACATGCAATTACATA

NGA6 GACTAAAGTGGGTCCCTTGG 112-175 (GA)31 3CACACCCAAAACTCGTAAAGC

NGA172 CATCCGAATGCCATTGTTC 132-194 (AG)27 3AGCTGCTTCCTTATAGCGTCC

BSAT4.001 GGTTTGATGATATTGTTTTGGC 184-244 (AT)13G(AAC)16 4ATTTTCTATTTTTATGATCGGGC

BSAT4.002 CATACCGATTTAGTTGTGGTTG 144-204 (AT)32 4TTGTTCGATTTTGTAGTTGTTAGC

BSAT4.006 TTAAGCGAAAGCGTGTGAG 72-160 (AT)37 4CTAGATCGTCCCTCGTCTGA

BSAT4.013 AGTTCCACCGAATAAAATAACG 169-206 (AT)25 4AAAGAAAAATCAGAAGATCCTACG

BSAT4.009 AGAAGCCCCTATGAGATGGT 182-330 (AG)28 4TGAGGGAGTTAGGTAGCAACA

BSAT4.011 TAGTTAATCTGAGTGTCTTCCGC 193-258 (AT)28 4CATGTCGATTGGGAAAAAAT

MSAT4.25 GAATGGTTGTTGATAGTTGA 198-229 (T)36 4AAATTTCAGGAGGTGATAGA

MSAT4.10 GGTAATTTTCTTGGAGACCC 164-258 (AT)37 4CTAACTAGATCGTCCCTCGT

MSAT4.33 TTCTTTGACACGCAAACA 104-206 (AG)19 4TGGTGACATAGACCCAATG

MSAT4.31 AGGGATATGGATTGAGA 186-400 (AG)24 4GCCGTATAACTATTGGTT

NGA8 TGGCTTTCGTTTATAAACATCC 124-250 (GA)28 4GAGGGCAAATCTTTATTTCGG

NGA1107 CGACGAATCGACAGAATTAGG 313-319 (CT) 27 4GGCTACAATAGTGGGAAAACG

BSAT5.002 AAACGAAGACGAAGAAGTCG 180-224 (AG)26 5GTTCTTGACCACGACGAAGT

BSAT5.008 TACCTAGACCACCAACCACC 126-170 (TA)23 5ATCTCATATTCTTGTGGGTATGTG

MSAT5.23 TTGATCCAATAAGTGCTG 126-280 (GA)42 5CCTAAACACCCTCAACTA

MSAT5.7 GCTTGTACATTACCGGACACC 220-272 (AT)29 5CCAAGCGTACTCGATCATGT

MSAT5.4 TCAACCCTAGATGGTGTCGAT 205-261 (AT)27 5TTCAATCATTTTTGCCGTGA

NGA139 GGTTTCGTTTCACTATCCAGG 136-212 (CT)33 5AGAGCTACCAGATCCGATGG

NGA151 CCAGAGCTTGTTTTGGGAAG 102-150 (CT)31 5TTTGATGAAACGGAATATAGAAAGC

Table A.2 MSAP-PCR primers used in the study.Marker Primer sequence (5’ – 3’)

AP2 GTAAGACTCTGCCGGAP3 TCCGATGCTGCCGGAP4 GGTGGTGATGCCGGMLG2 AACCCTCACCCTAACCCCGG

Fig. A.1 Growth performance of Arabidopsis seedlings exposed to 0 - 8.0 mg·L -1 Cd. (A) Arabidopsis seedlings exposed to 0, 0.25 (B), 1.0 (C), 4.0 (D), 5.0 (E) and 8.0 mg·L -1 Cd (F).

0 0.25 1.0 4.0 5.00

5

10

15

20

25

30

A

B

Cd concentration（ mg·L-1）

Freq

uenc

y

Fig. A.2 Polymorphism variations detected by RAPD from Arabidopsis plantlets exposed to 0 - 5.0 mg·L-1 Cd. (A) Arabidopsis seedlings exposed to Cd for 21 d and (B) 15 d.