artificial dna in living cells - home | national academies
TRANSCRIPT
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Artificial DNA in Living Cells
Floyd E. Romesberg, Ph.D.
National Academy of Sciences Forum on Synthetic Biology
19 September 2014
Washington, DC
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• Efforts to expand the genetic
alphabet by unnatural base pair
design and polymerase evolution
Chemical Biology and Biophysics
• Novel evolution-inspired
approaches to antibiotic
discovery
• Biophysical characterization
of how protein dynamics are
evolved for biological
function
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Expansion of the Genetic Alphabet/Code
B
B
B B DNA Polymerase RNA Polymerase
AA*
B
B
AA*
Seminal work of Steve Benner (FAME) Peter Schultz (TSRI)
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Predominantly Hydrophobic Nucleotides
(sugar & phosphate not shown)
S
BTp
O
BFr
NH
IN
NN
N
BTz NAP 2MN 3MN DMN 1HN 2HN
OHHO
N
O
O
7OFP
N
O
S
4TFP
N
O
O
4OFP
N
S
O
7OTP
N
S
O
4OTP
N
O
S
7TFP
NN N
N
NN
N
NNNN
NN
N NN
7AI PP ImPyNeb M7AI 4M7AI
NN N
N
NNN
P7AI PM7AI PPP
N
Np 8Q
N ON O
PICS
N O
PIM
N O
N
PNICS ICS
N O
5MICS
N O
MICS
N S
SICS
N O
N
NICS
N O
N
oNICS
N S
N
SNICS
N S
SoNICS
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Assays: Thermostability and Kinetics
0.33
0.34
0.35
0.36
0.37
0.38
0.39
0 40 50 60 70 80 90
A 260
T, ºC
5’-GCGTACXCATGCG-3’ 3’-CGCATGYGTACGC-5’
X N
Extension
Incorporation
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Structure of a First Generation
Hydrophobic UBP
With D.Wemmer (UC Berkeley) and P. Schultz (TSRI)
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Second Generation Hydrophobic Nucleotides
BEN MM1 MM3MM2 DM3 DM5DM4DM2
F
2FB 4FB
F
F
3FB
F
2,3DFB 2M3FB3,4DFB
F
3,5DFB
F
F
F
DM TM2TM
3F4MB TFB
F
F
FF F
F
2Br 3Br 2CN4Br 4CN
N
N
3Py2Py3CN
N
4Py
N
PYR
Br
Br
Br
CN
CN
CN
O N
3MP
N
4MP
N N
4MOP5MP
N N
4FP
N
5FP3FP
N
3MOP
F
F
O
O
O O O
O
O O O
FO
3CB
Cl
2OMB
3OMB MMO24OMB DMOMMO1
O
O
O
O O
O
O
S
MTp
S
ETp
S
MOTp
O
MFr
S
Tp
O
O
DMFr
TMB
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SAR Issue
X
dYTP insertion
dNTP (extension dY:dX)
X Y
Efficient extension: Primer terminus dY–minor groove hydrogen-bond acceptor
Template dX–minor groove hydrophobic group
Efficient dYTP Insertion: dYTP–minor groove hydrophobic group
template dX–minor groove hydrophobic group
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Two Screens of 3600 Candidate UBPs
Extension Screen
Full Length Screen
X
X
XY
+dNTPs
+dNTPs dYTP
+SYBR
+SYBR
XY
X
SYBR
SYBRSYBR
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A Family of UBPs that are Efficiently
Replicated and Transcribed in Vitro
<<
<
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Sequencing Analysis of Replication Fidelity
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Calibration Curve for
d5SICS-dNaM Replication
1 0 % natu ral
L R
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OneTaq PCR with d5SICS-dNaM
DNA Amplification Efficiency (%) Fidelity (%)
ACT b GTG 2.5×1012 97 99.945 ± 0.016
GTC b GGT 1.5×1012 95 99.874 ± 0.035
AGC b CGT 3.5×1012 96 99.930 ± 0.003
CCG b GAA 8.1×1012 >99 99.664 ± 0.037
GTA b TGT 3.1×1012 95 99.987 ± 0.021
AGA b AGT 8.5×1012 >99 >99.98
CCT b AAA 8.4×1012 >99 99.866 ± 0.014
GGT b TCC 2.6×1012 94 99.958 ± 0.012
ACT b b GTG 3.8×1012 96 ≈ 99.5
ACT b A b GTG 2.7×1012 94 ≈ 99.5
ACT b GTGACT b GTG 2.0×1012 93 99.47
NNN b NNN 4.8×1012 97 99.925 ± 0.008
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PCR Selection
X = 5SICS
Y = NaM
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Single Nucleotide Frequency
dNaM dNaM dNaM dNaM
Nucleotide position relative to dNaM
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Single Nucleotide Frequency
24.7%
18.7%
Nucleotide position relative to dNaM
dNaM dNaM dNaM dNaM
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Correlation Analysis
dNaM dNaM dNaM dNaM
Nucleotide position relative to dNaM
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Dinucleotide Frequency
X X X
X X
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Dinucleotide Frequency
3.5%
2.3%
X X
X X
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Structural Studies of d5SICS-dNaM
With Tammy Dwyer (U. San Diego)
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Structural Studies of d5SICS-dNaM:
Two Questions
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With K. Betz & A. Marx
Structural Studies of d5SICS-dNaM
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Structural Studies of d5SICS-dNaM
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Expansion of the Genetic Alphabet/Code
B
B
B B DNA Polymerase RNA Polymerase
AA*
B
B
AA*
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Extracellular Degradation
of dNaM and d5SICS
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Activity of Phaeodactylum tricornutum NTT
(PtNTT2) in E. coli (dATP uptake)
Transporter 1
Transporter 2
Transporter 3
Transporter 4
Transporter 5
Transporter 6
Transporter 7
Transporter 8
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PtNTT2-Mediated Uptake in E. coli
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pACS and pINF (X=NaM, Y=TPT3)
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d5SICS-dNaM and dTPT3-dNaM
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The Experiment
Transform E. coli with pACS, induce PtNTT2, add dXTPs,
transform with pINF, grow, recover pINF and characterize
Controls: transform with pUC19 instead of pINF
do not induce PtNTT2
do not add dXTPs
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Analysis of pINF After Growth in E. coli
(15 h, 22 Doublings, 107-Fold Amplification)
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Sequencing Analysis of pINF After Growth in E. coli
(15 h, 22 Doublings, 107-Fold Amplification)
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Global Nucleoside Content in pINF and
pUC19 via LC-MS/MS
With Ivan Correa, Nan Dai, and Jeremy Foster (NEB)
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Expansion of the Genetic Alphabet/Code
B
B
B B DNA Polymerase RNA Polymerase
AA*
B
B
AA*
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Reddit AMA
• 773 comments; score of 3056 (93% upvoted)
• Much of the dialogue was technical, unlike what was generated by the
NYT and NPR stories
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Jim Thomas Comments on
Legal/Regulatory Implications
“The arrival of this unprecedented ‘alien’ life
form could in time have far-reaching ethical,
legal and regulatory implications,” Jim Thomas
of the ETC Group, a Canadian advocacy
organization, said in an email. “While synthetic
biologists invent new ways to monkey with the
fundamentals of life, governments haven’t
even been able to cobble together the basics of
oversight, assessment or regulation for this
surging field.”
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Comments in Response to
Pollack’s NYT Article
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Comments in Response to
Pollack’s NYT Article
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Comments in Response NPR Health Blog
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Objections to Comparison with Nature
Imagine writing a story with a language with only four
letters. A fifth and sixth letter would let you write more
Interesting stories.
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Objections to Comparison with Nature
Imagine writing a story with a language with only four
letters. A fifth and sixth letter would let you write more
Interesting stories.
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