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EDGE®
Automated Extraction System
Method Note Compendium
Updated February 20, 2019
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Agriculture
Avocados 2
Beans 3
Brazil Nuts 4
Cacao 5
Cannabis Plant (Cannabinoids) 6
Cannabis Plant (Mycotoxins) 7
Cannabis Plant (Pesticides) 8
Dry Fish Samples 9
Dry Soy Meal & Fish Meal 10
Dry Spice Samples 11
Hazel Nuts 12
Hops 13
Pumpkin Seeds 14
Rat Poison Powder 15
Rice 16
Strawberries 17
Wet Feed/ Food Samples (PCBs) 18
Wet Feed/ Food Samples (Dioxins) 19
Wet Fish Tissue 20
Wet Food Sample 21
Total Fat From Feed/Food 22
Cannabis
Cannabis Plant (Cannabinoids) 24
Cannabis Plant (Mycotoxins) 25
Cannabis Plant (Pesticides) 26
Environmental & Regulatory
Environmental Samples 28
EPA 3545A 29
Large PUF Filters 30
Small PUF Filters 31
Wet Fish Tissue 32
PUF Filters 33
Soil, Loam or Clay (PAHs) 34
Soil, Loam, or Clay (PCBs) 35
Soil, Loam, or Clay (Dioxins) 36
Food Testing
Environmental Samples 38
EPA 3545A 39
Film Powder 40
Plastics 41
Avocados 42
Beans 43
Brazil Nuts 44
Cacao 45
Cannabis Plant (Cannabinoids) 46
Cannabis Plant (Mycotoxins) 47
Cannabis Plant (Pesticides) 48
Cashews 49
Dry Fish Samples 50
Dry Soy Meal & Fish Meal 51
Dry Spice Samples 52
Echinacea 53
Egg Powder 54
Hazel Nuts 55
Hops 56
Large PUF Filters 57
Mouthwash 58
PET 59
Contents
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Pigment 60
Polyethylene Powder 61
Polyethylene Resin 62
Polypropylene Powder 63
Polyvinyl Chloride 64
Protein Powder 65
Pumpkin Seeds 66
Rat Poison Powder 67
Resin 68
Rice 69
Small PUF Filters 70
Soil 71
Strawberries 72
Wet Feed/Food Samples (PCBs) 73
Wet Feed/ Food Samples (Dioxins) 74
Wet Fish Tissue 75
Wet Food Sample 76
XAD Resin 77
Yerba Mate 78
Molybdenum 79
Total Fat From Feed/Food 80
Total Fat From High-Fat Meats 81
Total Fat From Hotdogs 82
Total Fat From Peanuts 83
Total Fat From Pepperoni 84
Total Fat From Potted Meat 85
Total Fat From Protein Powder 86
PUF Filters 87
Soil, Loam, or Clay (PAHs) 88
Soil, Loam, or Clay (PCBs) 89
Soil, Loam, or Clay (Dioxins) 90
Toothpaste 91
Total Fat From Chocolate 92
Total Fat From Cookies 93
Geoscience & Mining
Molybdenum 95
Industrial Hygiene
Large PUF Filters 97
Rat Poison Powder 98
Small PUF Filters 99
PUF Filters 100
Materials Science
Film Powder 102
Plastics 103
Large PUF Filters 104
Polypropylene Powder 105
Polyvinyl Chloride 106
Rat Poison Powder 107
Resin 108
Small PUF Filters 109
XAD Resin 110
PUF Filters 111
Metals & Alloys
Molybdenum 113
Nutraceuticals
Dry Fish Samples 115
Echinacea 116
Protein Powder 117
Wet Fish Tissue 118
Yerba Mate 119
Total Fat From Protein Powder 120
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Personal Care Products
Mouthwash 122
Toothpaste 123
Plastics, Polymers, & Oils
Film Powder 125
Plastics 126
PET 127
Pigment 128
Polyethylene Powder 129
Polyethylene Resin 130
Polypropylene Powder 131
Polyvinyl Chloride 132
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Agriculture
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Extraction of PesticidesFrom AvocadosFrom Avocados
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc (textured side up) on top of the
C9 Q-Disc. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
SolventsAcetonitrile
Sorbents- Sodium Sulfate (5 g)- Magnesium Sulfate (1,200 mg)- Primary Second Amine (50 mg)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10020 01:00010
Stage Solvent Volume (mL)
1 Water 15
2 Acetonitrile 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PolyphenolsFrom BeansFrom Beans
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.1 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
Solvents(50:50) Methanol: Water
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 8020 10:001010
Stage Solvent Volume (mL)
1 (50:50) Methanol: Water 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of FatFrom Brazil NutsFrom Brazil Nuts
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight12 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (12 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 17510 00:00010
Stage Solvent Volume (mL)
1 Petroleum Ether 20
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PolyphenolsFrom CacaoFrom Cacao
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.1 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
Solvents(50:50) Methanol: Water
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 8020 10:001010
Stage Solvent Volume (mL)
1 (50:50) Methanol: Water 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of CannabinoidsFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TesEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Weigh the sample into the Q-Cup.
SolventsMethanol
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10010 0:3055
Stage Solvent Volume (mL)
1 Methanol 20
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of MycotoxinsFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TesEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1.5 g
Q-DiscᵀᴹC4
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsMethanol May also use a percentage of Water:Methanol as needed
SorbentsNone
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 5015 02:0055
Stage Solvent Volume (mL)
1 Methanol 10
2 Methanol 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PesticidesFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TesEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1.5 g
Q-DiscᵀᴹM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc on top of the C9 Q-Disc.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the salt. No mixing is required.
Solvents1% Acetic Acid in Acetonitrile
SorbentsContents of Agilent QuEChERS SPE 15 mL Tube
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 02:0055
Stage Solvent Volume (mL)
1 1% Acetic Acid in Acetonitrile 15
2 1% Acetic Acid in Acetonitrile 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PAHsFrom Dry Fish SamplesFrom Dry Fish Samples
Agriculture, Food Testing, NutraEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™.
2. Weight the sorbent in the Q-Cup. Tap lightly to spread evenly. 3. Weigh the sample into the Q-Cup layering the sample on top of the salt. No mixing is required.
SolventsDichloromethane
SorbentsC18 (1.7 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 03:001010
Stage Solvent Volume (mL)
1 Dichloromethane 15
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PCBsFrom Dry Soy Meal & Fish MealFrom Dry Soy Meal & Fish Meal
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹC1M1
EquipmentEDGE40 mL collection vials - clear
NotesLess than 5 grams of sample can be used with a ratio of 1:2 C18: Sample Either C1 or M1 Q-Disc can be used
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the salt. No mixing is required.
SolventsOptions:Dichloromethane(50:50) Hexane: Acetone
SorbentsC18 (2.5 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 03:001010
Stage Solvent Volume (mL)
1 Dichloromethane 15
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PesticidesFrom Dry Spice SamplesFrom Dry Spice Samples
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesMatrix matched calibration curve required without the use of sorbents.
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs.2. Weigh the sample into the Q-Cup.
SolventsAcetonitrile with 1% Acetic Acid
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4015 03:00510
Stage Solvent Volume (mL)
1 Acetonitrile with 1% Acetic Acid 10
2 Acetonitrile with 1% Acetic Acid 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of FatFrom Hazel NutsFrom Hazel Nuts
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight12 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (12 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 17510 00:00010
Stage Solvent Volume (mL)
1 Petroleum Ether 20
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PesticidesFrom HopsFrom Hops
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.5 g
Q-DiscᵀᴹM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesCanadian Pesticide Mix 4 in LCMS grade obtained from SPEX CertiPrep.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc (textured side up) on top of the
C9 Q-Disc.2. Add sorbents to bottom of Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping. No mixing is required.
3. Weigh the sample into the Q-Cup. Add 5.0 g hops on top of sorbent bed. 4. Spike with 100 ug/mL Canadian Pesticide Mix 4 and leave in hood for approximately 30 minutes to evaporate solvent.
Solvents1% Acetic Acid in Acetonitrile
Sorbents-ACS grade magnesium sulfate anhydrous > 99.5% (1.25 g)-Supelᵀᴹ QuE Citrate (1.25 g)-Primary secondary amine (1.25 g)-Siliabond C18 (1.25 g)*Different sorbents can be used
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10015 00:30510
Stage Solvent Volume (mL)
1 1% Acetic Acid in Acetonitrile 20
2 1% Acetic Acid in Acetonitrile 20
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of FatFrom Pumpkin SeedsFrom Pumpkin Seeds
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight12 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (12 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 17510 00:00010
Stage Solvent Volume (mL)
1 Petroleum Ether 20
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of BrodifacoumFrom Rat Poison PowderFrom Rat Poison Powder
Agriculture, Industrial Hygiene, EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsMethanol
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 13015 02:001010
2 13015 02:001010
Stage Solvent Volume (mL)
1 Methanol 15
2 Methanol 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PesticidesFrom RiceFrom Rice
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the salt. No mixing is required.
SolventsAcetonitile
Sorbents- Sodium Sulfate (5 g)- Magnesium Sulfate (1,200 mg)- Primary Second Amine (50 mg)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10020 01:00010
Stage Solvent Volume (mL)
1 Acetonitile 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PesticidesFrom StrawberriesFrom Strawberries
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the salt. No mixing is required.
SolventsAcetonitrile
Sorbents- Sodium Sulfate (5 g)- Magnesium Sulfate (1,200 mg)- Primary Second Amine (50 mg)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10020 01:00010
Stage Solvent Volume (mL)
1 Water 15
2 Acetonitrile 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PCBsFrom Wet Feed/ Food SamplesFrom Wet Feed/ Food Samples
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsDichloromethane
Sorbents-Sodium Sulfate (2.5 g)-C18 (2 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 13520 02:00510
Stage Solvent Volume (mL)
1 Water 15
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of DioxinsFrom Wet Feed/ Food SamplesFrom Wet Feed/ Food Samples
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsDichloromethane
Sorbents-Sodium Sulfate (2.5 g)-C18 (2 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 15020 02:00510
Stage Solvent Volume (mL)
1 Water 15
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PAHsFrom Wet Fish TissueFrom Wet Fish Tissue
Agriculture, Environmental and EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesIf there is moisture in collection vial after the extraction:-Using a glass funnel with glass wool and about 20 g of Sodium Sulfate. Pour over into new collection vial and rinse thoroughly with fresh solvent.
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsOptions:-Dichloromethane-(50:50) Hexane: Acetone
Sorbents- Sodium Sulfate (1 g)- C18 (0.8 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 03:001010
Stage Solvent Volume (mL)
1 Water 15
2 Extraction Solvent 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PesticidesFrom Wet Food SampleFrom Wet Food Sample
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the salt. No mixing is required.
SolventsAcetonitrile
Sorbents- Sodium Sulfate (5 g)- Magnesium Sulfate (1,200 mg)- Primary Second Amine (50 mg)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10020 02:00010
Stage Solvent Volume (mL)
1 Water 30
2 Acetonitrile 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extractionof Total Fat From Feed/Foodof Total Fat From Feed/Food
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight≤ 5 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.4. Dry Q-Cups with sample and sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.
SolventsPetroleum Ether
SorbentsWet Samples Only: Sand (use equal amount to sample size)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 01:00010
2 13010 01:00010
Stage Solvent Volume (mL)
1 Water 15
2 Petroleum Ether 20
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Cannabis
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Extraction of CannabinoidsFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TesEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Weigh the sample into the Q-Cup.
SolventsMethanol
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10010 0:3055
Stage Solvent Volume (mL)
1 Methanol 20
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of MycotoxinsFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TesEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1.5 g
Q-DiscᵀᴹC4
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsMethanol May also use a percentage of Water:Methanol as needed
SorbentsNone
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 5015 02:0055
Stage Solvent Volume (mL)
1 Methanol 10
2 Methanol 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PesticidesFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TesEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1.5 g
Q-DiscᵀᴹM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc on top of the C9 Q-Disc.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the salt. No mixing is required.
Solvents1% Acetic Acid in Acetonitrile
SorbentsContents of Agilent QuEChERS SPE 15 mL Tube
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 02:0055
Stage Solvent Volume (mL)
1 1% Acetic Acid in Acetonitrile 15
2 1% Acetic Acid in Acetonitrile 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Environmental & Regulatory
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Extraction of SVOCsEnvironmental SamplesEnvironmental Samples
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight30 g
Q-DiscᵀᴹC4
EquipmentEDGE40 mL collection vials - clear
NotesThis is a general method for the miscellaneous extraction of environmental samples.
Procedure 1.Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2.Weigh the sample into the Q-Cup.
SolventsOptions:AcetoneDichloromethaneHexaneToluene
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 00:001010
Stage Solvent Volume (mL)
1 Dichloromethane 10
2 Extraction Solvent 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of SVOCsEPA 3545AEPA 3545A
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight30 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesSee the CEM Application note: Extraction of Semi-Volatile Organic Compounds from Soil in Accordance with EPA 3545A if needed.
Procedure 1.Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2.Weigh the sample into the Q-Cup.
Solvents(50:50) Acetone: Hexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 01:001010
Stage Solvent Volume (mL)
1 Acetone 10
2 (50:50) Acetone: Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PCB'sFrom Large PUF FiltersFrom Large PUF Filters
Environmental and Regulatory, IEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup™ with the Q-Disc™. 2. Compress and push the filter into the base of the Q-Cup.
SolventsHexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 11520 03:00155
Stage Solvent Volume (mL)
1 Hexane 10
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PCB'sFrom Small PUF FiltersFrom Small PUF Filters
Environmental and Regulatory, IEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup™ with the Q-Disc™. 2. Compress and push the filter into the base of the Q-Cup.
SolventsHexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10015 00:10150
Stage Solvent Volume (mL)
1 Hexane 10
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PAHsFrom Wet Fish TissueFrom Wet Fish Tissue
Agriculture, Environmental and EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesIf there is moisture in collection vial after the extraction:-Using a glass funnel with glass wool and about 20 g of Sodium Sulfate. Pour over into new collection vial and rinse thoroughly with fresh solvent.
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsOptions:-Dichloromethane-(50:50) Hexane: Acetone
Sorbents- Sodium Sulfate (1 g)- C18 (0.8 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 03:001010
Stage Solvent Volume (mL)
1 Water 15
2 Extraction Solvent 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Cleaning ofPUF FiltersPUF Filters
Environmental and Regulatory, IEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup™ with the Q-Disc™. 2. Compress and push the filter into the base of the Q-Cup.
SolventsStage 1: AcetoneStage 2: Hexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 11515 03:00150
2 11523 03:00200
Stage Solvent Volume (mL)
1 Acetone 15
2 Hexane 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PAHs FromSoil, Loam, or Clay
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight30 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
Solvents(50:50) Acetone: Hexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 01:001010
Stage Solvent Volume (mL)
1 Acetone 10
2 Acetone 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PCBs FromSoil, Loam, or Clay
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight30 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
Solvents(50:50) Acetone: Hexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 01:001010
Stage Solvent Volume (mL)
1 Acetone 10
2 Acetone 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of Dioxins FromSoil, Loam, or Clay
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight30 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsToluene
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 15015 03:001010
Stage Solvent Volume (mL)
1 Acetone 10
2 Acetone 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Food Testing
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Extraction of SVOCsEnvironmental SamplesEnvironmental Samples
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight30 g
Q-DiscᵀᴹC4
EquipmentEDGE40 mL collection vials - clear
NotesThis is a general method for the miscellaneous extraction of environmental samples.
Procedure 1.Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2.Weigh the sample into the Q-Cup.
SolventsOptions:AcetoneDichloromethaneHexaneToluene
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 00:001010
Stage Solvent Volume (mL)
1 Dichloromethane 10
2 Extraction Solvent 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of SVOCsEPA 3545AEPA 3545A
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight30 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesSee the CEM Application note: Extraction of Semi-Volatile Organic Compounds from Soil in Accordance with EPA 3545A if needed.
Procedure 1.Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2.Weigh the sample into the Q-Cup.
Solvents(50:50) Acetone: Hexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 01:001010
Stage Solvent Volume (mL)
1 Acetone 10
2 (50:50) Acetone: Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction ofFilm PowderFilm Powder
Materials Science, Plastics, PolyEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹM1
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsDichloromethane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *40-7015 10:0005
Stage Solvent Volume (mL)
1 Dichloromethane 10
2 Cyclohexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PigmentsFrom PlasticsFrom Plastics
Materials Science, Plastics, PolyEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹM1
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsDichloromethane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *40-8015 10:0005
Stage Solvent Volume (mL)
1 Dichloromethane 10
2 Cyclohexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PesticidesFrom AvocadosFrom Avocados
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc (textured side up) on top of the
C9 Q-Disc. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
SolventsAcetonitrile
Sorbents- Sodium Sulfate (5 g)- Magnesium Sulfate (1,200 mg)- Primary Second Amine (50 mg)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10020 01:00010
Stage Solvent Volume (mL)
1 Water 15
2 Acetonitrile 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PolyphenolsFrom BeansFrom Beans
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.1 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
Solvents(50:50) Methanol: Water
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 8020 10:001010
Stage Solvent Volume (mL)
1 (50:50) Methanol: Water 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of FatFrom Brazil NutsFrom Brazil Nuts
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight12 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (12 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 17510 00:00010
Stage Solvent Volume (mL)
1 Petroleum Ether 20
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PolyphenolsFrom CacaoFrom Cacao
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.1 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
Solvents(50:50) Methanol: Water
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 8020 10:001010
Stage Solvent Volume (mL)
1 (50:50) Methanol: Water 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of CannabinoidsFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TesEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Weigh the sample into the Q-Cup.
SolventsMethanol
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10010 0:3055
Stage Solvent Volume (mL)
1 Methanol 20
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of MycotoxinsFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TesEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1.5 g
Q-DiscᵀᴹC4
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsMethanol May also use a percentage of Water:Methanol as needed
SorbentsNone
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 5015 02:0055
Stage Solvent Volume (mL)
1 Methanol 10
2 Methanol 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PesticidesFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TesEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1.5 g
Q-DiscᵀᴹM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc on top of the C9 Q-Disc.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the salt. No mixing is required.
Solvents1% Acetic Acid in Acetonitrile
SorbentsContents of Agilent QuEChERS SPE 15 mL Tube
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 02:0055
Stage Solvent Volume (mL)
1 1% Acetic Acid in Acetonitrile 15
2 1% Acetic Acid in Acetonitrile 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of FatFrom CashewsFrom Cashews
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight12 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (12 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 17510 00:00010
Stage Solvent Volume (mL)
1 Petroleum Ether 20
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PAHsFrom Dry Fish SamplesFrom Dry Fish Samples
Agriculture, Food Testing, NutraEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™.
2. Weight the sorbent in the Q-Cup. Tap lightly to spread evenly. 3. Weigh the sample into the Q-Cup layering the sample on top of the salt. No mixing is required.
SolventsDichloromethane
SorbentsC18 (1.7 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 03:001010
Stage Solvent Volume (mL)
1 Dichloromethane 15
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PCBsFrom Dry Soy Meal & Fish MealFrom Dry Soy Meal & Fish Meal
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹC1M1
EquipmentEDGE40 mL collection vials - clear
NotesLess than 5 grams of sample can be used with a ratio of 1:2 C18: Sample Either C1 or M1 Q-Disc can be used
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the salt. No mixing is required.
SolventsOptions:Dichloromethane(50:50) Hexane: Acetone
SorbentsC18 (2.5 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 03:001010
Stage Solvent Volume (mL)
1 Dichloromethane 15
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PesticidesFrom Dry Spice SamplesFrom Dry Spice Samples
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesMatrix matched calibration curve required without the use of sorbents.
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs.2. Weigh the sample into the Q-Cup.
SolventsAcetonitrile with 1% Acetic Acid
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4015 03:00510
Stage Solvent Volume (mL)
1 Acetonitrile with 1% Acetic Acid 10
2 Acetonitrile with 1% Acetic Acid 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of FlavonoidsFrom EchinaceaFrom Echinacea
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Weigh the sample into the Q-Cup.
Solvents(70:30) Methanol: Water
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 6020 01:00020
Stage Solvent Volume (mL)
1 (70:30) Methanol: Water 10
2 (70:30) Methanol: Water 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of FatFrom Egg PowderFrom Egg Powder
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsToluene
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 17515 01:001010
Stage Solvent Volume (mL)
1 Toluene 15
2 Toluene 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of FatFrom Hazel NutsFrom Hazel Nuts
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight12 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (12 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 17510 00:00010
Stage Solvent Volume (mL)
1 Petroleum Ether 20
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PesticidesFrom HopsFrom Hops
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.5 g
Q-DiscᵀᴹM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesCanadian Pesticide Mix 4 in LCMS grade obtained from SPEX CertiPrep.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc (textured side up) on top of the
C9 Q-Disc.2. Add sorbents to bottom of Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping. No mixing is required.
3. Weigh the sample into the Q-Cup. Add 5.0 g hops on top of sorbent bed. 4. Spike with 100 ug/mL Canadian Pesticide Mix 4 and leave in hood for approximately 30 minutes to evaporate solvent.
Solvents1% Acetic Acid in Acetonitrile
Sorbents-ACS grade magnesium sulfate anhydrous > 99.5% (1.25 g)-Supelᵀᴹ QuE Citrate (1.25 g)-Primary secondary amine (1.25 g)-Siliabond C18 (1.25 g)*Different sorbents can be used
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10015 00:30510
Stage Solvent Volume (mL)
1 1% Acetic Acid in Acetonitrile 20
2 1% Acetic Acid in Acetonitrile 20
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PCBsFrom Large PUF Filters
Environmental and Regulatory, IEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup™ with the Q-Disc™. 2. Compress and push the filter into the base of the Q-Cup.
SolventsHexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 11520 03:00155
Stage Solvent Volume (mL)
1 Hexane 10
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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ExtractionFrom MouthwashFrom Mouthwash
Personal Care (Health and BeauEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs.
2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping. 3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsHexane
SorbentsOptions:-Sodium Polyacrylate (5 g)-Diatomaceous Earth (5 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 03:00010
Stage Solvent Volume (mL)
1 Hexane 10
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of AdditivesFrom PETFrom PET
Plastics, Polymers, and OilsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
Solvents(40:60) Acetone: Hexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 115*25 05:00510
Stage Solvent Volume (mL)
1 Acetone 15
2 Hexane 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of AdditivesFrom PigmentFrom Pigment
Plastics, Polymers, and OilsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsDimethylformamide
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10020 01:001010
Stage Solvent Volume (mL)
1 Dimethylformamide 10
2 Dimethylformamide 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of AdditivesFrom Polyethylene Powder
Plastics, Polymers, and OilsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹM1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the C9 Q-Disc on top of the M1 Q-Disc.
2. Weigh the sample into the Q-Cup.
SolventsIsopropanol
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *9030 15:00010
Stage Solvent Volume (mL)
1 Isopropanol 15
2 Isopropanol 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of Chemical ModifierFrom Polyethylene ResinFrom Polyethylene Resin
Plastics, Polymers, and OilsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.1 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
Solvents(40:60) Acetone:Hexane
SorbentsNone
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *6020 15:001010
Stage Solvent Volume (mL)
1 (40:60) Acetone:Hexane 10
2 (40:60) Acetone:Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of AdditivesFrom Polypropylene Powder
Materials Science, Plastics, PolyEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs.
2. Weigh the sample into the Q-Cup.
SolventsIsopropanol
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12030 15:00010
Stage Solvent Volume (mL)
1 Isopropanol 15
2 Isopropanol 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PhthalatesFrom Polyvinyl ChlorideFrom Polyvinyl Chloride
Materials Science, Plastics, PolyEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
Solvents(50:50) Isopropanol: Cyclohexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *8020 10:00010
Stage Solvent Volume (mL)
1 (50:50) Isopropanol: Cyclohexane 10
2 (50:50) Isopropanol: Cyclohexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of NutrientsFrom Protein PowderFrom Protein Powder
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC3
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
Solvents(80:20) Methanol:Water
SorbentsSand (3 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 15015 00:001010
Stage Solvent Volume (mL)
1 (80:20) Methanol:Water 15
2 (80:20) Methanol:Water 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of FatFrom Pumpkin SeedsFrom Pumpkin Seeds
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight12 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (12 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 17510 00:00010
Stage Solvent Volume (mL)
1 Petroleum Ether 20
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of BrodifacoumFrom Rat Poison PowderFrom Rat Poison Powder
Agriculture, Industrial Hygiene, EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsMethanol
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 13015 02:001010
2 13015 02:001010
Stage Solvent Volume (mL)
1 Methanol 15
2 Methanol 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of AntioxidantsFrom ResinFrom Resin
Materials ScienceEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsMethanol
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10015 02:00510
Stage Solvent Volume (mL)
1 Methanol 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PesticidesFrom RiceFrom Rice
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the salt. No mixing is required.
SolventsAcetonitile
Sorbents- Sodium Sulfate (5 g)- Magnesium Sulfate (1,200 mg)- Primary Second Amine (50 mg)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10020 01:00010
Stage Solvent Volume (mL)
1 Acetonitile 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PCB'sFrom Small PUF FiltersFrom Small PUF Filters
Environmental and Regulatory, IEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup™ with the Q-Disc™. 2. Compress and push the filter into the base of the Q-Cup.
SolventsHexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10015 00:10150
Stage Solvent Volume (mL)
1 Hexane 10
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of DioxinsFrom SoilFrom Soil
EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight10 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™.
2. Weigh the sample into the Q-Cup.
SolventsToluene
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 17515 01:001010
Stage Solvent Volume (mL)
1 Toluene 10
2 Toluene 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Bring to volume or evaporate as necessary for analysis.
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Extraction of PesticidesFrom StrawberriesFrom Strawberries
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the salt. No mixing is required.
SolventsAcetonitrile
Sorbents- Sodium Sulfate (5 g)- Magnesium Sulfate (1,200 mg)- Primary Second Amine (50 mg)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10020 01:00010
Stage Solvent Volume (mL)
1 Water 15
2 Acetonitrile 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PCBsFrom Wet Feed/ Food SamplesFrom Wet Feed/ Food Samples
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsDichloromethane
Sorbents-Sodium Sulfate (2.5 g)-C18 (2 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 13520 02:00510
Stage Solvent Volume (mL)
1 Water 15
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of DioxinsFrom Wet Feed/ Food SamplesFrom Wet Feed/ Food Samples
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsDichloromethane
Sorbents-Sodium Sulfate (2.5 g)-C18 (2 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 15020 02:00510
Stage Solvent Volume (mL)
1 Water 15
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PAHsFrom Wet Fish TissueFrom Wet Fish Tissue
Agriculture, Environmental and EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesIf there is moisture in collection vial after the extraction:-Using a glass funnel with glass wool and about 20 g of Sodium Sulfate. Pour over into new collection vial and rinse thoroughly with fresh solvent.
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsOptions:-Dichloromethane-(50:50) Hexane: Acetone
Sorbents- Sodium Sulfate (1 g)- C18 (0.8 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 03:001010
Stage Solvent Volume (mL)
1 Water 15
2 Extraction Solvent 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PesticidesFrom Wet Food SampleFrom Wet Food Sample
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the salt. No mixing is required.
SolventsAcetonitrile
Sorbents- Sodium Sulfate (5 g)- Magnesium Sulfate (1,200 mg)- Primary Second Amine (50 mg)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10020 02:00010
Stage Solvent Volume (mL)
1 Water 30
2 Acetonitrile 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PAH'sFrom XAD ResinFrom XAD Resin
Materials ScienceEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight15 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesBe sure that sample or sand is present in the Q-Cup when running this method. Sample size does matter, as you decrease the sample size you will build up more pressure within the cell.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™.
2. Weigh the sample into the Q-Cup.
SolventsDichloromethane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 18020 00:001010
Stage Solvent Volume (mL)
1 Dichloromethane 15
2 Dichloromethane 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PAH'sFrom Yerba MateFrom Yerba Mate
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.5 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsOptions:(50:50) Hexane: AcetoneDichloromethaneAcetone
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 120-15015 01:00105
Stage Solvent Volume (mL)
1 Solvent used in extraction 10
2 Solvent used in extraction 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction ofMolybdenumMolybdenum
Geoscience and Mining, Metals EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsAcetone
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14015 01:00105
Stage Solvent Volume (mL)
1 Acetone 10
2 Acetone 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extractionof Total Fat From Feed/Foodof Total Fat From Feed/Food
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight≤ 5 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.4. Dry Q-Cups with sample and sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.
SolventsPetroleum Ether
SorbentsWet Samples Only: Sand (use equal amount to sample size)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 01:00010
2 13010 01:00010
Stage Solvent Volume (mL)
1 Water 15
2 Petroleum Ether 20
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extractionof Total Fat From High-Fat Meatsof Total Fat From High-Fat Meats
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.4. Dry Q-Cups with sample and sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.
SolventsPetroleum Ether
SorbentsSand (2 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 01:00010
2 13010 01:00010
Stage Solvent Volume (mL)
1 Water 10
2 Petroleum Ether 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extractionof Total Fat From Hotdogsof Total Fat From Hotdogs
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.4. Dry Q-Cups with sample and sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.
SolventsPetroleum Ether
SorbentsSand (2 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 01:00010
2 13010 01:00010
Stage Solvent Volume (mL)
1 Water 10
2 Petroleum Ether 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extractionof Total Fat From Peanutsof Total Fat From Peanuts
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight12 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.4. Dry Q-Cups with sample and sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.
SolventsPetroleum Ether
SorbentsSand (12 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 15015 00:00010
Stage Solvent Volume (mL)
1 Petroleum Ether 20
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extractionof Total Fat From Pepperoniof Total Fat From Pepperoni
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.4. Dry Q-Cups with sample and sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.
SolventsPetroleum Ether
SorbentsSand (2 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 01:00010
2 13010 01:00010
Stage Solvent Volume (mL)
1 Water 10
2 Petroleum Ether 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extractionof Total Fat From Potted Meatof Total Fat From Potted Meat
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.4. Dry Q-Cups with sample and sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.
SolventsPetroleum Ether
SorbentsSand (2 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 01:00010
2 13010 01:00010
Stage Solvent Volume (mL)
1 Water 10
2 Petroleum Ether 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extractionof Total Fat From Protein Powderof Total Fat From Protein Powder
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (6 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 02:00105
Stage Solvent Volume (mL)
1 Petroleum Ether 10
2 Petroleum Ether 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Cleaning ofPUF FiltersPUF Filters
Environmental and Regulatory, IEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup™ with the Q-Disc™. 2. Compress and push the filter into the base of the Q-Cup.
SolventsStage 1: AcetoneStage 2: Hexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 11515 03:00150
2 11523 03:00200
Stage Solvent Volume (mL)
1 Acetone 15
2 Hexane 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PAHs FromSoil, Loam, or Clay
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight30 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
Solvents(50:50) Acetone: Hexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 01:001010
Stage Solvent Volume (mL)
1 Acetone 10
2 Acetone 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PCBs FromSoil, Loam, or Clay
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight30 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
Solvents(50:50) Acetone: Hexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 01:001010
Stage Solvent Volume (mL)
1 Acetone 10
2 Acetone 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of Dioxins FromSoil, Loam, or Clay
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight30 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsToluene
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 15015 03:001010
Stage Solvent Volume (mL)
1 Acetone 10
2 Acetone 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction ofToothpasteToothpaste
Personal Care (Health and BeauEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes* for sand- mix into sample* for Diatamaceous Earth- layer under sample, do not mix.
Procedure1. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sorbent/sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup*.
SolventsHexane
SorbentsOptions:Sand (15 g)Diatamaceous Earth (5 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 03:00010
Stage Solvent Volume (mL)
1 Hexane 10
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Hydrolysis & Extraction ofTotal Fat From ChocolateTotal Fat From Chocolate
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesStep 2- EDGE Method1. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs. 2. Place the Filter Paper into the Q-Cup.
ProcedureStep 1- Hydrolysis Method 1. Weigh out 1 g of milled or ground sample. 2. Add 1 mL Ethanol and 5 mL (25:11) Concentrated Hydrochloric Acid: Water. Heat for 1 hr at 100° C in a covered dish. 3. Add 5 mL of Ethanol. Filter the sample using Whatman 3 Filter Paper with a filter funnel, a vacuum pump, and filter flask. Wash any residual material from the beaker in the filter funnel using Ethanol. 4. Dry the filter paper in an oven at 100° C for 30 minutes. After the drying step, place Filter Paper into the Q-Cup and extract.
SolventsPetroleum Ether(25:11) Concentrated Hydrochloric Acid: Water
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 01:00010
2 13010 01:00010
Stage Solvent Volume (mL)
1 Water 15
2 Petroleum Ether 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Hydrolysis & Extraction ofTotal Fat From CookiesTotal Fat From Cookies
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesStep 2- EDGE Method1. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs. 2. Place the Filter Paper into the Q-Cup.
ProcedureStep 1- Hydrolysis Method 1. Weigh out 1 g of milled or ground sample. 2. Add 1 mL Ethanol and 5 mL (25:11) Concentrated Hydrochloric Acid: Water. Heat for 1 hr at 100° C in a covered dish. 3. Add 5 mL of Ethanol. Filter the sample using Whatman 3 Filter Paper with a filter funnel, a vacuum pump, and filter flask. Wash any residual material from the beaker in the filter funnel using Ethanol. 4. Dry the filter paper in an oven at 100° C for 30 minutes. After the drying step, place Filter Paper into the Q-Cup and extract.
SolventsPetroleum Ether(25:11) Concentrated Hyrdochloric Acid: Water
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 01:00010
2 13010 01:00010
Stage Solvent Volume (mL)
1 Water 15
2 Petroleum Ether 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Geoscience & Mining
94
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Extraction ofMolybdenumMolybdenum
Geoscience and Mining, Metals EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsAcetone
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14015 01:00105
Stage Solvent Volume (mL)
1 Acetone 10
2 Acetone 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
9995
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Industrial Hygiene
96
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Extraction of PCB'sFrom Large PUF FiltersFrom Large PUF Filters
Environmental and Regulatory, IEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup™ with the Q-Disc™. 2. Compress and push the filter into the base of the Q-Cup.
SolventsHexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 11520 03:00155
Stage Solvent Volume (mL)
1 Hexane 10
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
11097
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Extraction of BrodifacoumFrom Rat Poison PowderFrom Rat Poison Powder
Agriculture, Industrial Hygiene, EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsMethanol
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 13015 02:001010
2 13015 02:001010
Stage Solvent Volume (mL)
1 Methanol 15
2 Methanol 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
9898
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Extraction of PCB'sFrom Small PUF FiltersFrom Small PUF Filters
Environmental and Regulatory, IEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup™ with the Q-Disc™. 2. Compress and push the filter into the base of the Q-Cup.
SolventsHexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10015 00:10150
Stage Solvent Volume (mL)
1 Hexane 10
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
10999
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Cleaning ofPUF FiltersPUF Filters
Environmental and Regulatory, IEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup™ with the Q-Disc™. 2. Compress and push the filter into the base of the Q-Cup.
SolventsStage 1: AcetoneStage 2: Hexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 11515 03:00150
2 11523 03:00200
Stage Solvent Volume (mL)
1 Acetone 15
2 Hexane 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
108100
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Materials Science
101
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Extraction ofFilm PowderFilm Powder
Materials Science, Plastics, PolyEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹM1
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsDichloromethane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *40-7015 10:0005
Stage Solvent Volume (mL)
1 Dichloromethane 10
2 Cyclohexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
93102
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Extraction of PigmentsFrom PlasticsFrom Plastics
Materials Science, Plastics, PolyEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹM1
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsDichloromethane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *40-8015 10:0005
Stage Solvent Volume (mL)
1 Dichloromethane 10
2 Cyclohexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
94103
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Extraction of PCB'sFrom Large PUF FiltersFrom Large PUF Filters
Environmental and Regulatory, IEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup™ with the Q-Disc™. 2. Compress and push the filter into the base of the Q-Cup.
SolventsHexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 11520 03:00155
Stage Solvent Volume (mL)
1 Hexane 10
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
110104
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Extraction of AdditivesPolypropylene Powder
Materials Science, Plastics, PolyEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs.
2. Weigh the sample into the Q-Cup.
SolventsIsopropanol
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12030 15:00010
Stage Solvent Volume (mL)
1 Isopropanol 15
2 Isopropanol 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
96105
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Extraction of PhthalatesFrom Polyvinyl ChlorideFrom Polyvinyl Chloride
Materials Science, Plastics, PolyEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
Solvents(50:50) Isopropanol: Cyclohexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *8020 10:00010
Stage Solvent Volume (mL)
1 (50:50) Isopropanol: Cyclohexane 10
2 (50:50) Isopropanol: Cyclohexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
90106
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Extraction of BrodifacoumFrom Rat Poison PowderFrom Rat Poison Powder
Agriculture, Industrial Hygiene, EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsMethanol
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 13015 02:001010
2 13015 02:001010
Stage Solvent Volume (mL)
1 Methanol 15
2 Methanol 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
98107
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Extraction of AntioxidantsFrom ResinFrom Resin
Materials ScienceEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsMethanol
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10015 02:00510
Stage Solvent Volume (mL)
1 Methanol 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
106108
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Extraction of PCB'sFrom Small PUF FiltersFrom Small PUF Filters
Environmental and Regulatory, IEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup™ with the Q-Disc™. 2. Compress and push the filter into the base of the Q-Cup.
SolventsHexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10015 00:10150
Stage Solvent Volume (mL)
1 Hexane 10
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
109109
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Extraction of PAH'sFrom XAD ResinFrom XAD Resin
Materials ScienceEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight15 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesBe sure that sample or sand is present in the Q-Cup when running this method. Sample size does matter, as you decrease the sample size you will build up more pressure within the cell.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™.
2. Weigh the sample into the Q-Cup.
SolventsDichloromethane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 18020 00:001010
Stage Solvent Volume (mL)
1 Dichloromethane 15
2 Dichloromethane 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
101110
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Cleaning ofPUF FiltersPUF Filters
Environmental and Regulatory, IEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup™ with the Q-Disc™. 2. Compress and push the filter into the base of the Q-Cup.
SolventsStage 1: AcetoneStage 2: Hexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 11515 03:00150
2 11523 03:00200
Stage Solvent Volume (mL)
1 Acetone 15
2 Hexane 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
108111
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Metals & Alloys
112
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Extraction ofMolybdenumMolybdenum
Geoscience and Mining, Metals EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsAcetone
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14015 01:00105
Stage Solvent Volume (mL)
1 Acetone 10
2 Acetone 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
99113
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Nutraceuticals
114
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Extraction of PAHsFrom Dry Fish SamplesFrom Dry Fish Samples
Agriculture, Food Testing, NutraEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™.
2. Weight the sorbent in the Q-Cup. Tap lightly to spread evenly. 3. Weigh the sample into the Q-Cup layering the sample on top of the salt. No mixing is required.
SolventsDichloromethane
SorbentsC18 (1.7 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 03:001010
Stage Solvent Volume (mL)
1 Dichloromethane 15
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of FlavonoidsFrom EchinaceaFrom Echinacea
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Weigh the sample into the Q-Cup.
Solvents(70:30) Methanol: Water
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 6020 01:00020
Stage Solvent Volume (mL)
1 (70:30) Methanol: Water 10
2 (70:30) Methanol: Water 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of NutrientsFrom Protein PowderFrom Protein Powder
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC3
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
Solvents(80:20) Methanol:Water
SorbentsSand (3 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 15015 00:001010
Stage Solvent Volume (mL)
1 (80:20) Methanol:Water 15
2 (80:20) Methanol:Water 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PAHsFrom Wet Fish TissueFrom Wet Fish Tissue
Agriculture, Environmental and EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesIf there is moisture in collection vial after the extraction:-Using a glass funnel with glass wool and about 20 g of Sodium Sulfate. Pour over into new collection vial and rinse thoroughly with fresh solvent.
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsOptions:-Dichloromethane-(50:50) Hexane: Acetone
Sorbents- Sodium Sulfate (1 g)- C18 (0.8 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 03:001010
Stage Solvent Volume (mL)
1 Water 15
2 Extraction Solvent 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PAH'sFrom Yerba MateFrom Yerba Mate
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.5 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsOptions:(50:50) Hexane: AcetoneDichloromethaneAcetone
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 120-15015 01:00105
Stage Solvent Volume (mL)
1 Solvent used in extraction 10
2 Solvent used in extraction 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extractionof Total Fat From Protein Powderof Total Fat From Protein Powder
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (6 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 02:00105
Stage Solvent Volume (mL)
1 Petroleum Ether 10
2 Petroleum Ether 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Personal Care Products
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ExtractionFrom MouthwashFrom Mouthwash
Personal Care (Health and BeauEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs.
2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping. 3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsHexane
SorbentsOptions:-Sodium Polyacrylate (5 g)-Diatomaceous Earth (5 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 03:00010
Stage Solvent Volume (mL)
1 Hexane 10
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction ofToothpasteToothpaste
Personal Care (Health and BeauEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes* for sand- mix into sample* for Diatamaceous Earth- layer under sample, do not mix.
Procedure1. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sorbent/sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup*.
SolventsHexane
SorbentsOptions:Sand (15 g)Diatamaceous Earth (5 g)
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 03:00010
Stage Solvent Volume (mL)
1 Hexane 10
2 Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Plastics, Polymers, & Oils
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Extraction ofFilm PowderFilm Powder
Materials Science, Plastics, PolyEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹM1
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsDichloromethane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *40-7015 10:0005
Stage Solvent Volume (mL)
1 Dichloromethane 10
2 Cyclohexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PigmentsFrom PlasticsFrom Plastics
Materials Science, Plastics, PolyEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹM1
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsDichloromethane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *40-8015 10:0005
Stage Solvent Volume (mL)
1 Dichloromethane 10
2 Cyclohexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of AdditivesFrom PETFrom PET
Plastics, Polymers, and OilsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
Solvents(40:60) Acetone: Hexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 115*25 05:00510
Stage Solvent Volume (mL)
1 Acetone 15
2 Hexane 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of AdditivesFrom PigmentFrom Pigment
Plastics, Polymers, and OilsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
SolventsDimethylformamide
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10020 01:001010
Stage Solvent Volume (mL)
1 Dimethylformamide 10
2 Dimethylformamide 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of AdditivesFrom Polyethylene Powder
Plastics, Polymers, and OilsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹM1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Layer the C9 Q-Disc on top of the M1 Q-Disc.
2. Weigh the sample into the Q-Cup.
SolventsIsopropanol
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *9030 15:00010
Stage Solvent Volume (mL)
1 Isopropanol 15
2 Isopropanol 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of Chemical ModifierFrom Polyethylene ResinFrom Polyethylene Resin
Plastics, Polymers, and OilsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.1 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
Solvents(40:60) Acetone:Hexane
SorbentsNone
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *6020 15:001010
Stage Solvent Volume (mL)
1 (40:60) Acetone:Hexane 10
2 (40:60) Acetone:Hexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of AdditivesFrom Polypropylene Powder
Materials Science, Plastics, PolyEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. Sandwich the G1 disc between the two C9 discs.
2. Weigh the sample into the Q-Cup.
SolventsIsopropanol
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12030 15:00010
Stage Solvent Volume (mL)
1 Isopropanol 15
2 Isopropanol 15
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
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Extraction of PhthalatesFrom Polyvinyl ChlorideFrom Polyvinyl Chloride
Materials Science, Plastics, PolyEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscᵀᴹC1
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.
Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill or cryomill the sample. Assemble the Q-Cup™ with the Q-Disc™. 2. Weigh the sample into the Q-Cup.
Solvents(50:50) Isopropanol: Cyclohexane
SorbentsN/A
Wash Program
Stage Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *8020 10:00010
Stage Solvent Volume (mL)
1 (50:50) Isopropanol: Cyclohexane 10
2 (50:50) Isopropanol: Cyclohexane 10
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
90132