automated sampling - securecell · • dilution from 1:2 to 1:30 • reagent addition, mixing and...
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Reliable around the clock!Automated Sampling
MODULARITY IN THE STANDARD SET-UP
NUMERA® AUTOMATED SAMPLING SYSTEM Numera® allows drawing low volume samples from up to 16 bioreactors. The samples can be automatically preprocessed and stored
at chilled conditions. Preprocessing includes dilution, reagent addition and filtration. In addition to the standard modules, an advanced
set-up includes the Routing Module to allow direct injection into various analzyers and increases the user‘s flexibility.
Numera® offers 5 standard modules that can be combined according to your specific needs. Preprocessing, such as cell removal, is
performed in the dedicated module respectively.
Multiplexer Module• Interface to up to 16 bioreactors
• Sterility barrier (pinch valve)
• EtOH cleaning to prevent cross-contaminations
• Back-flush with sterile air to
minimize sample volume (2-4 mL)
Filtration Module• Cell removal and retention of supernatant
• Unique tape filtration technology
• New filter for every sample
• No clogging, no cross-contamination
• 0.22, 0.45, 0.65 and 1.2 µm pore size
Dilution Module• Dilution from 1:2 to 1:30
• Reagent addition, mixing and setting of reaction time
• Overall precision of ≤ 2% SD, max. accuracy
MUX SDU TFU SRU ASXMUX SDU TFU SRU ASXMUX SDU TFU SRU ASX
MUX SDU TFU SRU ASX
Autosampler• Storage of samples in chilled environment
• Different sizes available (48-320 sample positions)
• Usable with deep well plates or vials
Control Module• Preparation of the system
• Configuration of basic parameters
• Automated sampling 24/7• High frequency and low volume sampling• No operator-to-operator variations• Low maintenance effort
• Consistent cell removal and maximum sample yield• No clogging, no cross-contamination • Option to directly integrate common analyzers
BENEFITS OF NUMERA®
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Close the loop with an advanced set-up!
Automated Sampling & Analytics
CLOSE THE LOOPThe combination of the automated sampling system, Numera®, and the Process Information Management System, Lucullus®, enables
the automation of the whole workflow covering sampling, measurement, process monitoring and control.
In combination with Lucullus, all activities such as sampling, data alignment and analytics are optimized. By using predefined
and customized sampling schedules, Lucullus coordinates the interaction between bioreactor, Numera® and analytical devices. All
analytical data is automatically fed back into Lucullus® and assigned to the correct process and time. Hence, Numera® and Lucullus®
enable monitoring and control of process parameters (e.g. CPPs) without manual interaction.
The Routing Module, a novel core part of Numera®, enables the integration of various analyzers and adds
extra flexibility. Numera integrates up to 16 bioreactors or downstream devices, 4 different third-party
analyzers and an HPLC. It allows fast and direct transfer of biomass-containing or cell-free samples to
analytical devices or to the autosampler in order to be stored. For each sample and analyzer, the processing
steps within Numera® can be individually customized.
INTEGRATION OF ANALYZERS
THE SMART SAMPLING WORKFLOW
• High-frequency and low volume sampling• High-precision sample preparation• On-line measurements with various analyzers
• Automated data import from analyzers• Real-time process evaluation• Feedback control-loops
NUMERA® & LUCULLUS®: A UNIQUE ALL-IN-ONE SOLUTION
Request a visit to one of our demolabs and more information:
MUX SDU TFU SRU ASX
AUTOMATED SAMPLINGSAMPLING PLAN AUTOMATED TRANSFERTO ANALAYZER
AUTOMATED MEASUREMENT & DATA TRANSFER
•••••••
BioProfile® FLEX2™ Cedex® BioHT Cedex HiRes VI-Cell XRAgilent HPLC Thermo Scientfic HPLCOthers on request
Reactor 1
Reactor 1
Reactor 2
Reactor 3
Reactor 4
...
Reactor N
R1_S1
R2_S1
R3_S1
R4_S1
...
RN_SM
REACTOR SAMPLE ID VCD Glc Numera
3 x 106
3,5 x 106
Drawing
Planned
Planned
15,5
15,8
...
...
.........
... ...
SAMPLING & ANALYTICAL DATA
Integrated Analyzers
VCD
conc
t
Glc