automation-ready purification chemistries · 2020-03-13 · in-lab automation . support available ....

SV 96 Vacuum-Based Chemistries

ReliaPrep™, MagneSil®, simplyRNA & MagneHis™ Paramagnetic Particle-based Chemistries

Plasmid DNA Wizard® MagneSil™ Plasmid Purification System Wizard MagneSil Tfx™ System Wizard® SV 96 Plasmid DNA Purification System

Genomic DNA ReliaPrep™ 96 gDNA Miniprep HT System ReliaPrep™ Large Volume HT gDNA Isolation System Wizard® SV 96 Genomic DNA Purification System Wizard® Magnetic 96 DNA Plant System MagneSil® Blood Genomic, Max Yield System

Total RNA

ReliaPrep™ simplyRNA HT System, Custom MagneSil® Total RNA mini-Isolation System

DNA Fragments

Wizard® SV 96 PCR Clean-Up System

Recombinant Protein

MagneHis™ Protein Purification System

Automation-Ready Purification Chemistries

Product Cat. # Size

Wizard® MagneSil® Plasmid Purification System

A1630 4 x 96 preps

A1631 8 x 96 preps

Wizard® MagneSil® Plasmid Purification System, HTP1

A1635 100 x 96 preps

Each catalog item contains sufficient material for indicated number of preps: • Cell Resuspension Solution • Cell Lysis Solution

•Neutralization Solution •MagneSil® BLUE •MagneSil® RED

• Elution Buffer • Collection Plates

MagneBot, MagneSil and Wizard are trademarks of Promega Corporation. Products may be covered by existing or

pending patents. Please visit www.promega.com for more information.

Wizard® MagneSil® Plasmid Purification System Provides a simple and reliable method for rapid isolation of plasmid DNA using silica-coated paramagnetic particles in a 96-well, high-throughput format without centrifugation or vacuum manifolds.

• Automation Friendly: Methods available for liquid handlers including Beckman Coulter and Tecan. Learn more at www.promega.com/automethods.

• Rapid Results: Process a full plate in ~1 hour (96-well head) to ~1½ hours (8-channel).

Isolated plasmid DNA ready for use:

•Sequencing •PCR •Transformation

Custom & Bulk

Available

In-Lab Automation

Support Available

Plasmid DNA

96-well

Produce Cleared Lysate with

MagneSil® Blue

Recover Plasmid

Capture Plasmid with

Magnesil® Red

Capture & release washes with 80% ethanol

•Probe Generation •Restriction Digestion •Cloning

Product Cat. # Size

Wizard MagneSil Tfx™ System A2380 4 x 96 preps

Contains sufficient material for indicated number of preps: • Cell Resuspension Solution • Cell Lysis Solution

•Neutralization Solution •MagneSil® BLUE • Endotoxin Removal Resin

•MagneSil® RED • 4/40 Wash Solution •Nuclease-Free Water

MagneBot is a trademark and Wizard MagneSil Tfx is a trademark of Promega Corporation. Products may be covered by

existing or pending patents. Please visit www.promega.com for more information.

Wizard MagneSil Tfx™ System Provides a simple and reliable method for rapid isolation of transfection-quality plasmid DNA using silica-coated paramagnetic particles in a 96-well, high-throughput format without centrifugation or vacuum manifolds.

• Automation Friendly: Methods available for liquid handlers including Beckman Coulter. Learn more at www.promega.com/automethods.

• Rapid Results: Process a full plate in ~1 hour (96-well head) to ~1½ hours (8-channel).

Custom & Bulk

Available

In-Lab Automation

Support Available

Plasmid DNA

96-well

Produce Cleared Lysate with

MagneSil® Blue

Recover Plasmid

Capture Plasmid with

Magnesil® Red


Treat cleared lysate with Endotoxin Removal Resin

Capture & release wash with 4/40 Wash Solution

Isolated plasmid DNA ready for use:

•Transfection •Sequencing •PCR •Transformation

•In vitro transcription •Probe Generation •Restriction Digestion •Cloning

Wizard® SV 96 Plasmid DNA Purification System

Product Cat. # Size


A2255 5 x 96 preps


A2258 100 x 96

preps

Vac-Man® 96 Vacuum Manifold A2291 Each

Provides a simple and reliable method for rapid isolation of plasmid DNA using silica membranes in a 96-well, high-throughput format.

• Automation Friendly: Methods available for liquid handlers that can use vacuum methods including Beckman Coulter, Caliper, Eppendorf, PerkinElmer and Tecan. Learn more at www.promega.com/automethods.

• Rapid Results: Process a full plate in 60 minutes or less

Crude Lysate

filter

cleared lysate

Binding Membrane

waste

Neutralize Wash

Apply Vacuum

Apply Vacuum

Apply Vacuum

Apply Vacuum

Dry Elute

Apply Vacuum

Elution plate

.

.

. . .

.

. . . . . . .

. .

. . .

2X

Lysate Clearing

Plate

Binding Plate

Reagents supplied to make crude lysate

Cat. #A2255 contains all items listed below. Cat. #A2258 contains only the underlined items. •Deep-Well Plate (for culturing bacteria) • Cell Resuspension Solution

• Cell Lysis Solution •Neutralization Solution • Lysate Clearing Plates

• Binding Plates •Wash Solution •Nuclease-Free Water

• Elution Plates • Plate Sealers •Alkaline Protease Solution (optional use)

Wizard and Vac-Man are trademarks of Promega Corporation. Products may be covered by existing or pending patents.

Please visit www.promega.com for more information.

Custom & Bulk

Available

In-Lab Automation

Support Available

Plasmid DNA

Manual or

Automated

96-well

Isolated plasmid DNA ready for use: •Sequencing •PCR •Transformation •Probe Generation •Restriction Digestion •Cloning

ReliaPrep™ 96 gDNA Miniprep HT System

Product Cat. # Size

ReliaPrep™ 96 gDNA Miniprep HT System

A2670 1 x 96 preps

A2671 4 x 96 preps

Isolate genomic DNA (gDNA) from whole blood or saliva collected in Oragene®DISCOVER or Oragene®DNA collection devices. The system has been automated on third-party liquid-handlers, allowing walkaway purification of genomic DNA.

• Automation Ready: Methods available for multiple liquid handlers. Learn more at www.promega.com/automethods

• Rapid, Safe Results: Recover gDNA from 96 samples in only 2.5 hours minutes without the use of hazardous chemicals like phenol and chloroform. Ready for a variety of applications including PCR, qPCR, microarrays and next-gen sequencing.

Contains sufficient material for indicated number of preps: • Proteinase K Solution • Cell Lysis Buffer

• ReliaPrep™ Magnetic Resin • Binding Buffer •Wash Buffer

• 25mM Tris-HCl (pH 8.0) • 10mM EDTA (pH 8.0) • RNase A Solution (4mg/ml)

ReliaPrep is a trademark of Promega Corporation. Products may be covered by existing or pending patents. Please visit

www.promega.com for more information. Oragene is a registered trademark of DNA Genotek Inc.

Custom & Bulk

Available

Genomic DNA

Manual or

Automated

In-Lab Automation

Support Available

Capture gDNA with ReliaPrep™ Magnetic

Resin & Binding Buffer

Blood or saliva samples are lysed in Lysis Buffer plus

Proteinase K

Capture and release washes in Wash Buffer and then 50% ethanol

Recover gDNA in 25mM Tris-HCL Buffer

Isolated genomic DNA ready for use: •PCR & qPCR •Microarrays •Next-gen sequencing

96-well Ask about platforms

& configurations

ReliaPrep™ Large Volume HT gDNA Isolation System

Product Cat. # Size

ReliaPrep™ Large Volume HT gDNA Isolation System

A1751 96 x 10ml preps

ReliaPrep™ LV 32 HSM Instrument*

A1715 Each

Tecan Freedom EVO® – HSM Workstation

XAT1000 Each

Isolate genomic DNA (gDNA) from whole blood, regardless of sample storage or shipping conditions. The system has been automated on the Tecan Freedom EVO® Workstation, allowing walkaway purification of genomic DNA.

• Walkaway Automation: Load the instrument with samples, add the reagents, start the protocol, pure gDNA from up to 32 samples in less than 4 hours. No manual intervention.

• Reagent & Time Savings: Each sample can be a different volume between 1 and 10ml. Instrument detects blood volume in each sample and scales reagents accordingly.

Contains sufficient material for indicated number of preps: • Cell Lysis Buffer • Binding Buffer

• ReliaPrep™ Resin •Wash Buffer •Alkaline Protease

• Proteinase K Solution •Nuclease-Free Water

Service agreements available. ReliaPrep is a trademark of Promega Corporation. Products may be covered by existing or pending patents. Please visit www.promega.com for more information. Freedom EVO is a registered trademark of Tecan Group Ltd.

Custom & Bulk

Available

Genomic DNA

Automated

In-Lab Automation

Support Available

1-32 X

1 to 10ml Blood

Blood volume detected

Lysis Binding to Particles

Capture & release washes

Elute pure DNA

Tecan Freedom EVO®– HSM Workstation

Wizard® SV 96 Genomic DNA Purification System

Product Cat. # Size

Wizard® SV 96 Genomic DNA Purification System

A2371 4 x 96 preps

Vac-Man® 96 Vacuum Manifold A2291 Each

Proteinase K V3021 100mg

Provides a simple and reliable method for rapid isolation of genomic DNA from cultured cells or tissue lysates including rodent tail using silica membranes in a 96-well, high-throughput format.


• Rapid Results: Process a full plate in 30 minutes from cells or 45-60 minutes from tissue lysates digested with proteinase K.

Catalog #A2371 contains sufficient material for indicated number of preps: •Wizard® SV Lysis Buffer • Binding Plates

•Wizard® SV Wash Solution •Nuclease-Free Water • 96-well Deep Well Plates

• Tissue Digest Reagents (Nuclei Lysis Solution, RNase A Solution, 0.5M EDTA. Proteinase K available separately.)



Apply Vacuum

Dry Elute

Apply Vacuum

Elution plate

Transfer

Apply Vacuum

. .

Add Wizard® SV Lysis Buffer.

Mix by pipetting

PBS washed

cell monolayer

Culture plate

Wash

Apply Vacuum

. .

Binding Plate

waste

3X

Custom & Bulk

Available

Genomic DNA

Manual or

Automated

96-well

In-Lab Automation

Support Available

Isolated genomic DNA ready for use: •qPCR •PCR

96-well

Custom & Bulk

Available

Genomic DNA

Manual or

Automated

In-Lab Automation

Support Available

Wizard® Magnetic 96 DNA Plant System

Product Cat. # Size

Wizard® Magnetic 96 DNA Plant System

FF3760 2 x 96 preps

FF3761 4 x 96 preps

Provides a simple and reliable method for rapid isolation of genomic DNA from leaf tissue or seeds using silica-coated paramagnetic particles in a 96-well, high-throughput format . Procedure requires solubilization of sample with a bead beater or grinding apparatus.

• Automation Friendly: Methods available for liquid handlers including Beckman Coulter Biomek® FX. Learn more at www.promega.com/automethods

• Rapid, Safe Results: Recover gDNA in 40 minutes (96-well head) without lengthy incubations or hazardous chemicals like phenol and chloroform. Ready for amplification-based applications.

Contains sufficient material for indicated number of preps: • Lysis Buffer A • Lysis Buffer B

•Wash Buffer, Plant •MagneSil® Paramagnetic Particles • Collection Plate (4-pack) Wizard and MagneSil are registered trademarks of Promega Corporation. Products may be covered by existing or pending

patents. Please visit www.promega.com for more information. Biomek is a registered trademark of Beckman Coulter.

Capture gDNA with MagneSil®/Lysis

Buffer B

Plant leaf tissue or seeds homogenized

in Lysis Buffer A

Capture and release washes in

Wash Buffer, Plant

Recover gDNA

Isolated genomic DNA ready for use: •qPCR •PCR •RAPD

96-well

Custom & Bulk

Available

Genomic DNA

Automated

In-Lab Automation

Support Available

MagneSil® Blood Genomic, Max Yield System

Product Cat. # Size

MagneSil® Blood Genomic, Max Yield System

MD1360 1 x 96 preps

Provides a consistent and simple method for rapid isolation of high purity genomic DNA (gDNA) from 200µl of blood and other sample types. This system is particularly well suited to gDNA isolation from small numbers of cells.

• Automation Ready: Methods available for liquid handlers including Beckman Coulter Biomek® FX. Learn more at www.promega.com/automethods

• Rapid, Safe Results: Recover gDNA in 90 minutes without lengthy incubations or hazardous chemicals like phenol and chloroform. Ready for amplification-based applications.

Contains sufficient material for indicated number of preps: • Lysis Buffer, Blood • Salt Wash, Blood

•MagneSil® Paramagnetic Particles •Alcohol Wash, Blood • Elution Buffer, Blood

•Anti-Foam Reagent MagneSil is a registered trademarks of Promega Corporation. Products may be covered by existing or pending patents.


Capture gDNA with MagneSil® PMPs/ Lysis Buffer, Blood

Blood, cells, tissues, or bacterial samples are lysed

in Lysis Buffer, Blood

Capture and release washes in Lysis, Salt & Alcohol Washes, Blood

Recover gDNA in Elution

Buffer, Blood

Isolated genomic DNA ready for use: •PCR •qPCR •Multiplex PCR •SNP genotyping

ReliaPrep™ simplyRNA HT System, Custom

Product Cat. # Size

ReliaPrep™ simplyRNA HT System, Custom

X9601 4 x 96 preps

Provides a method for adapting the Maxwell® 16 simplyRNA chemistry to 96 well plate format on third party liquid handlers. Produces high purity, concentrated total RNA samples ready for a variety of downstream applications.

• Automation Ready: Current method available for the Beckman Coulter Biomek® FX liquid handler. Learn more at www.promega.com/automethods.

• Rapid Results: Process a full 96 well plate in only 75 minutes.

Each catalog item contains sufficient material for indicated number of preps: • RNA Resin ( Paramagnetic Particles, PMPs) • Binding Solution 1

• RNA DNase Wash • Binding Solution 2 •Wash Solution

•Homogenization Solution • Cell Lysis Buffer (CLD) •DNase I

• 1-Thioglycerol •Nuclease-Free Water • Plus other components

Maxwell is a registered trademark of Promega Corporation. Products may be covered by existing or pending patents.


Custom & Bulk

Available

Total RNA

Automated

In-Lab Automation

Support Available

Isolated total RNA ready for use: •RT-qPCR •Microarray •RT-PCR •1st Strand cDNA

Recover Total RNA

Capture RNA on simplyRNA PMPs


Cells or mammalian/ plant tissue in

Homogenization Buffer and then Lysis Buffer

Resuspend PMPs in DNase I Solution


Add DNase Stop Solution

96-well

Product Cat. # Size

MagneSil® Total RNA mini-Isolation System

Z3351 4 plates

Contains sufficient material for indicated number of plates whether 96- or 384-well format: •MagneSil® RNA Paramagnetic Particles • RNA Lysis Buffer

•DNase I (lyophilized) • Yellow Core Buffer (DNase I resuspension) •MnCl2 Solution (DNase I activation)

•DNase Stop Solution •Nuclease-Free Water

MagneSil is a registered trademark of Promega Corporation. Products may be covered by existing or pending patents.


MagneSil® Total RNA mini-Isolation System Provides a simple and reliable method for rapid isolation of total RNA from cultured cells, whole blood or tissue lysates using silica-coated paramagnetic particles in a 96-well or 384-well, high-throughput format without centrifugation or vacuum manifolds.

• Automation Friendly: Methods available for liquid handlers including Beckman Coulter. Learn more at www.promega.com/automethods.

• Rapid Results: Process a 96-well plate in 50 minutes (96-well head) to 80 minutes (8-channel). Process a 384-well plate in 90 minutes (96-well head).

96- & 384-well

Recover Total RNA

Capture RNA on MagneSil® RNA PMPs

Capture & release wash with

90% ethanol

Cells, Blood or Tissue homogenates in RNA Lysis Buffer

Resuspend PMPs in DNase I Solution

Isolated Total RNA ready for use:

•RT-qPCR •RT-PCR •1st Strand cDNA


Add DNase Stop Solution

Total RNA

Custom & Bulk

Available

In-Lab Automation

Support Available


Product Cat. # Size


A9341 4 x 96 preps

A9342 8 x 96 preps

A9345 100 x 96 preps

Vac-Man® 96 Vacuum Manifold

A2291 Each

Provides a simple and reliable method for rapid purification of 100bp to 10kb PCR product away from primers, primer dimers, and reactants using silica membranes in a 96-well, high-throughput format.


• Rapid Results: Process a full plate in 20 minutes with 90% recovery.

Catalog # A9341, A9342 and A9345 contains sufficient material for indicated number of preps:

•Membrane Binding Solution • Binding Plates •Nuclease-Free Water

• Collection Plates



Transfer Wash

Apply Vacuum

Apply Vacuum

Apply Vacuum

Dry Elute

Apply Vacuum

Elution plate

. .

. . .

3X Binding

Plate

Add equal volume

Membrane Binding Solution to PCR reaction

PCR Plate . .

waste

Custom & Bulk

Available

DNA Fragments

Manual or

Automated

96-well

In-Lab Automation

Support Available

Isolated PCR DNA ready for use: •Sequencing •Labeling •Restriction Digestion •Cloning •Microarray Analysis


Product Cat. # Size


V8550 325 preps

MagneHis™ Ni-Particles V8565 10ml

The MagneHis™ Protein Purification System provides a simple, rapid and reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins. Paramagnetic precharged nickel particles (MagneHis™ Ni-Particles) are used to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate in an automated procedure • Automation Friendly: Methods available for liquid handlers like Beckman-Coulter and

Tecan. Learn more at www.promega.com/automethods.

• Rapid Purification & High Recovery: Process a full plate in ~1 hour (96-well head) and MagneHis™ Ni-Particle bind ~1mg protein/ml.

Catalog # V8550 contains sufficient material for indicated number of preps:

•MagneHis™ Ni-Particles •MagneHis™ Binding/Wash

Solution •MagneHis™ Elution Buffer • FastBreak™ Cell Lysis Reagent

•DNase I (lyophilized) MagneHis is a trademark of Promega Corporation. Products may be covered by existing or pending patents. Please visit

www.promega.com for more information.

Custom & Bulk

Available

Recombinant Protein

Manual or

Automated

In-Lab Automation

Support Available

96-well

Capture polyhistidine protein with

MagneHis™ Ni-Particles

Bacterial, Insect, or Mammalian cells lysed with FastBreak™ Lysis

Buffer


Elute Recover polyhistidine

protein

www.promega.com/automethods

In-Lab Automation

Support Available

Verified methods for: • Nucleic acid purification • Recombinant protein purification • Cell-based assays

Variety of platforms*: • Aurora Discovery • Beckman Coulter • Caliper Life Sciences • CyBio • Deerac Fluidics • Eppendorf • PerkinElmer • Tecan

* Promega chemistries may not have automethods for every platform listed.

Screen capture of www.promega.com/automethods, November 2011.

Download ready-to-use automated methods for your instrument.

http://www.promega.com/automethods

Additional Field Support Services

Training (Cat. # S3001, S3002, S3003) Customized training assists you with implementation of Promega technologies in your laboratory. Training is available online (S3001), in your laboratory (S3002), or at a Promega facility (S3003). Integration Support (Cat. # S3004) Integration Support assists you in combining Promega technologies, such as those outlined here, with laboratory instrumentation, customizing protocols for new sample types, as well as improving laboratory efficiency. Applications Development (Cat. # S3005) Applications Development adapts Promega technologies to meet your new need such as the development of modified protocols that provide a solution for a previously unsupported application.

Scientists at Your Service

You’ll be surprised what we’ll do to help you succeed.

Have questions about automating Promega chemistries on or not on the www.promega.com/automethods list?

More on Integration Support

Are you currently running/investigating Promega chemistries & need further help

optimizing or implementing?

Need a custom automation method developed specific to

your research needs?

Don’t have the time or know-how to implement

Promega chemistries?

“What if…?”

…greater than 10 years of industry experience integrating chemistries and instruments in

high-throughput environments

Kevin Kershner 608-219-5126

Bob Ray 610-427-9338

Field Support Scientists

[email protected]

http://www.promega.com/automethods

Custom & Bulk Ordering

Need it Customized?

We can help! • Custom products

• Custom dispensing

• Bulk quantities

• OEM solutions, too

Ask your Promega Representative

or visit

www.promega.com/custom

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automation-ready purification chemistries · 2020-03-13 · in-lab automation . support available ....

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