av-mb-sop-004 (ip,usp) sop for identification of p.aeruginosa

7/27/2019 Av-mb-sop-004 (Ip,Usp) Sop for Identification of p.aeruginosa

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Department: Quality Assurance DOC. No. : AV/MB/SOP/004

Page No. : Page 1 of 3

Effective Date: 01.06.06 Revision No. : 00

Subect SOP for i!entification an! isolation of P.aeruginosa.

Prepare! "y Approve! "y Aut#ori$e! by

Microbiologist Management Representatie !actor" Manager

#$%ASS&!&'() &nternal *se Onl" #

RE%&S&ONS

Revision ' Revise! Pages Description Date

00 Original issue ().(*.(*


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Page No. : Page + of 3



).( Purpose

,o -efine proce-re for isolation of Pse-omonas aerginosa its

i-entification.

+.( Scope

,is proce-re is applicable to isolate an- i-entif" P.aerginosa.

,.( Responsibilities

Microbiologist

AM2alit"

-.( Definition :SOP) Stan-ar- operating proce-re.

.( Proce!ure

.) ,ransfer te antit" of te pretreate- preparation 10 gm or 10 ml

sample 5 0 ml bffer pospate 7.+ p89: e;amine- containing

1gm or 1ml of te pro-ct to 100 ml of fli- so"abean casein

-igest me-im.

.+ Mi; an- incbate at 3 ors.

., ';amine te me-im for gro?t an- if gro?t is present: strea@ a

portion of te me-im on te srface of cetrimi-e agar me-im:

eac plate- on Petri -ises..- $oer an- incbate at 3+4 ors.

. &f pon e;amination: none of te plates contains colonies aing

te caracteristics liste- in te table of te me-ia se-: te sample

meets te reirement for free-om from P.aerginosa.

.* &f an" colone" conforming to te -escription in table are pro-ce-:

carr" ot te o;i-ase an- pigment tests.

./ Strea@ representatie sspect colonies from te agar srface of

cetrimi-e agar me-im on te srface of Pse-omonas agar

me-im for te -etection of florescein an- pse-omonas agar

me-im for -etection of p"oc"anin containe- in Petri -ises.

.0 $oer an- inert te inoclate- me-ia an- incbate at 3337=$ fornot less tan 3 -a"s.

.1 ';amine te strea@e- srfaces n-er ltraiolet ligt. ';amine

te plates to -etermine ?eter colonies conforming to te

-escription gien in te table.





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.)( &f gro?t of sspect colonies occrs: place + or 3 -rops of afresl" prepare- 1 ?/ soltion of ::1:1:tetramet"l4

pen"lene-iamine -i"-roclori-e on filter paper an- smear ?it

te colon" or transfer colonies to strips or -is@s of filter paper tat

preiosl" as been impregnate- ?it :-imet"lp

pen"lene-iamine -i"-roclori-e if tere is no -eelopment of a

pin@ color :canging to prple: te sample meets te reirement

of te test for te absence of Pse-omonas aerginosa.

2able: 2ests for P. aeruginosa

3e!ium C#aracteristiccolonial

morp#ology

4luorescence in5% lig#t

O6i!ase test 7ram stain

Cetrimi!e agar Cenerall" greenis Creenis Positie egatie ro-s

Pseu!omonas

agar me!ium for

!etection of

fluorescein

Cenerall"

colorless to

"ello?is

Dello?is Positie egatie ro-s

Pseu!omonas

agar me!ium for!etect ion of

pyocyanin

Cenerall" greenis Ble Positie egatie ro-s

*.( Documentation :

Microbiological test report

/.( References:&P6/A116/VO%.&&




av-mb-sop-004 (ip,usp) sop for identification of p.aeruginosa

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