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winCATS Planar Chromatography Manager

User : camag Approved :. . . . . . . . . . . . . . . . . . . SN 1410W024, V1.4.3Saturday, January 01, 2005 12:50:43 AM Report ID : 07D5010107003215 Page 1 of 5

LABORATORIUM BIOFARMAKAFAKULTAS FARMASI

UNIVERSITAS HASANUDIN

Analysis Report

SOP documentValidated DesignDescription :

Analysis C:\CAMAG\winCATS\Data\Praktikan Fitokimia GolonganSenin 2013\Fito Kamis 2015\kelompok 6 Bayam duri.cna

Created/used by camag Saturday, January 01, 2005 12:50:21 AMCurrent user camag

Stationary phase

Executed by camag Saturday, January 01, 2005 12:44:08 AMPlate size (X x Y) 5.0 x 10.0 cmMaterial HPTLC plates silica gel 60 F 254Manufacturer E. MERCK KGaABatchGLP codePre-washing NoModification No

Definitions - Screening

Executed by camag Saturday, January 01, 2005 12:47:37 AM

Samples

awalHeksanBJA

Substance name Rf Window size ManufacturerBatch

number Expiry dateProductnumber

spot 1 0.94 0.500spot 2 0.75 0.500

Detection - CAMAG TLC Scanner 3

InformationApplication position 11.5 mmSolvent front position 94.0 mm

Instrument CAMAG TLC Scanner 3 "Scanner3_140717" S/N 140717 (1.14.28)Executed by camag Saturday, January 01, 2005 12:44:08 AMNumber of tracks 3Position of first track X 11.5 mmDistance between tracks 10.0 mmScan start pos. Y 9.0 mmScan end pos. Y 94.0 mmSlit dimensions 4.00 x 0.30 mm, MicroOptimize optical system LightScanning speed: 20 mm/sData resolution: 100 m/step



Measurement TableWavelength 254Lamp D2 & WMeasurement Type RemissionMeasurement Mode Absorption Optical filter Second orderDetector mode AutomaticPM high voltage 334 V

Detector propertiesY-position for 0 adjust 9.0 mmTrack # for 0 adjust 0Analog Offset 10%Sensitivity Automatic (29)

Integration

PropertiesData filtering Savitsky-Golay 7Baseline correction Lowest SlopePeak threshold min. slope 5Peak threshold min. height 10 AUPeak threshold min. area 50Peak threshold max. height 990 AUTrack start position 11.5 mmTrack end position 94.0 mmDisplay scaling Automatic

All tracks at Wavelength

1.00

0.60

0.40

0.20

0.00

[ Rf ]

500

400

350

300

250

200

150

100

50

0

[ AU ]

50

40

35

30

25

20

15

10

5

0

[ mm ]



Track 1, ID:

-0.14 0.06 0.26 0.46 0.66 0.86 1.060

50

100

150

200

250

300

350

400

450

500

-0.14 0.06 0.26 0.46 0.66 0.86 1.060

50

100

150

200

250

300

350

400

450

500

1spot 2 @ 254 nm

spot 1 @ 254 nm

PeakStart

RfStart

HeightMaxRf

MaxHeight

Max%

EndRf

EndHeight Area

Area% Assigned substance

1 0.71 3.6 0.72 13.5 6.56 0.73 10.4 131.3 1.58 unknown *2 0.73 11.2 0.75 26.5 12.84 0.78 3.9 692.3 8.35 spot 23 0.87 0.9 0.93 166.3 80.60 0.98 17.5 7465.3 90.06 spot 1

Track 2, ID:

-0.14 0.06 0.26 0.46 0.66 0.86 1.060

50

100

150

200

250

300

350

400

-0.14 0.06 0.26 0.46 0.66 0.86 1.060

50

100

150

200

250

300

350

400

1spot 1 @ 254 nm

PeakStart

RfStart

HeightMaxRf

MaxHeight

Max%

EndRf

EndHeight Area


1 0.86 6.7 0.92 131.1 49.80 0.93 130.0 3405.1 44.07 unknown *2 0.93 129.9 0.94 132.2 50.20 0.99 20.0 4322.0 55.93 spot 1

Track 3, ID:

-0.14 0.06 0.26 0.46 0.66 0.86 1.060

50

100

150

200

250

300

350

400

-0.14 0.06 0.26 0.46 0.66 0.86 1.060

50

100

150

200

250

300

350

400

spot 1 @ 254 nm

PeakStart

RfStart

HeightMaxRf

MaxHeight

Max%

EndRf

EndHeight Area


1 0.85 0.4 0.94 111.5 100.00 0.96 61.8 6625.4 100.00 spot 1



Spectrum scan

Executed by camag Saturday, January 01, 2005 12:50:21 AMMode All detected peaksSlit dimensions 6.00 x 0.30 mm, MicroOptimize optical system ResolutionScanning speed 100 nm/sData resolution 1 nm/stepReference spectrum, pos X 11.5 mmReference spectrum, pos Y 9.0 mm

Measurement TableLamp D2 & WStart wavelength 200 nmEnd wavelength 700 nmMeasurement type RemissionMeasurement Mode Absorption Optical filter Second orderDetector Mode Automatic

Detector propertiesY-position for 0 adjust 0.0 mmTrack # for 0 adjust 0Analog Offset 10%Sensitivity Automatic (25)

spot 1 on all Tracks

700.0600.0550.0500.0450.0400.0350.0300.0250.0200.0 [ nm ]

100.0

80.0

70.0

60.0

50.0

40.0

30.0

20.0

10.0

0.0

[ AU ]

100.0

80.0

70.0

60.0

50.0

40.0

30.0

20.0

10.0

0.0

[ AU ]

T Rf Substance Max. @1 0.93 Rf spot 1 200 nm2 0.94 Rf spot 1 200 nm3 0.94 Rf spot 1 200 nm

spot 2 on all Tracks

700.0600.0550.0500.0450.0400.0350.0300.0250.0200.0 [ nm ]

100.0

80.0

70.0

60.0

50.0

40.0

30.0

20.0

10.0

0.0

[ AU ]

100.0

80.0

70.0

60.0

50.0

40.0

30.0

20.0

10.0

0.0

[ AU ]

T Rf Substance Max. @1 0.75 Rf spot 2 200 nm



Evaluation results

Evaluation SequenceTrack Track type Vial Sample ID1 Sample 12 Sample 13 Sample 1

Table of substancesPosition Tracks

Substance MD mm 1 2 3spot 1 88.7 A A Aspot 2 73.2 A

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