best practices forbiobanking –examples from the leipzig ......also hairat roomtemperature leipzig...

Leipzig Research Center for Civilization Diseases

This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.

Best Practices for Biobanking– Examples from the Leipzig Medical Biobank

Dr. Ronny Baber

Biobanking 2019 BioTech Pharma Summit , February 15th 2019, Porto

Institute for Laboratory Medicine, Cinical Chemistry and Molecular Diagnostics

University Hospital Leipzig

Leipzig Medical Biobank –Leipzig Research Centre for Civilisation Diseases

University Leipzig



The Leipzig Research Center for Civilization Diseases (LIFE)



• Meeting der Geschäftsführer der Mitglieder der Landesexzellensinitiative Sachsen

• 22.02.2013

• Matthias Nüchter

Chemistry

Biochemistry

MPI

UFZ

Leipzig Research Center for Civilization Diseases (LIFE)



Population based Biobanking in LIFE

(A) LIFE HEALTH (B) LIFE DISEASES• ADULT Depression 40-60 (n=3.300)• Child Depression (n=760)• Heart (n=7.000)• Head and neck cancer (n=120)

• ADULT (n=10.000)• Child (n=6.000, incl. 1.000 newborns)

(Leipzig population based study)

Which genetic and environmental factors cause theindividual variance of modern diseases?



The largest LIFE Cohorts



Assessment in LIFE Child – a Selection

Poulin et al., Eur J Epidemiol, 2017

basal metabolic rateeye examination

questionaires

infant examinationdental examination

spirometry

singing & speaking voice

anthropometry

sport tests

ultra sound



Follow up of the Child Cohort

Poulin et al., Eur J Epidemiol, 2017



Assessment in LIFE Adult – a Selectionassessment examplesanthropometry 3D-bodyscan, height, weight

heart function echocardiography, heart ultrasound

vascular system carotid artery sonography, blood pressure

glucose metabolism oGTT, fasting glucose, HbA1c

physical activity, fitness and sleep 7-day actinometry

macula and glaucoma degeneration optical coherence tomography

neuroimaging and neurocognition MRI, cognitive tests

allergy prick-test

interviews, questionnaires smoking habit, nutrition, lifestyle

Loeffler et al., BMC Public Health, 2015



Follow up of the Adult cohort

Loeffler et al., BMC Public Health, 2015



LIFE Leipzig Heart studyCarotid ultrasound Angiography

Ankle-brachial Index

ECG/Stress test

Echocardiography

Roland-Ernst-Foundation LIFE

2006 2010 2016

CAD: coronary artery disease

MI: myocardial infarction

Beutner et al., 2011, PLoS one



Heart Study Follow Up

• Focus on patients without heart failure with preserved pump function

• Patients with complete biomarker and echocardigraphic data sets

• Surveys: cardiological complaints, cardiovascular events …

• Physical examinations: anthropometry, investigations of blood vessels and cardiological function...

Male Femalen 1030 506

Mean ± SD 66.2 ± 10.0 68.1 ± 10.1

Minimum Age 37.4 31.8

Maximum Age 96.3 92.8



General Workflow of Biosamples in LIFE



The LIFE-Biobank



Preanalytical WorkflowAutomatic Aliquoting

Cell Lab

Sample Elevator

Preanalytical Work Area

Straws

Cryo vials

TemporaryStorage

max. 120 min



Preanalytical Workflow

Blood Withdrawal immediate Registration and Centrifugation

Cloning

Adults

Children

max. 120 min

mean: 97 ± 51 min; 85.4 % within time

mean: 113 ± 59 min; 63.0 % within time

Parentsmean: 105 ± 51 min; 68.6 % within time

Freezing

Adult FUmean: 76 ± 26 min; 92.8 % within time



Biobank Workflow

Temporary Freezer

Transport (dry ice)

Sorting

Channel in

Storage (automatically)

Storage (manually)

20 min for 700 samples 8 min for 300 samples

Consistent cooling chain



Biological samples from study participants

- 80 C° < - 150 °CSerum x xPlasma (ETDA, Citrate, Li-Hep) xUrine x xBreast milk xDried blood spot cards xDNA/RNA xPeripheral mononuclear blood cells xStabilized whole blood xSaliva xStool x

also hair at room temperature



Laboratory information management system = CryoLab (in-house product)

Ambulance

(e.g. patient encoding)

Preanalytics

(e.g. sample storage time)

Sample

(e.g. material)

Biobank

(e.g. sample location)

CryoLab



From Population Based to Healthcare integrated Biobanking

Biobank



Leipzig Medical Biobank

LIFE EpidemiologicalBiobank

• Adults

• Children

LIFE Medical Biobank

• Heart

• Head/Neck Cancer

• Depression

Medical Biobank

• Cancer

• …

Healthcare Integrated Biobanking –Leipzig Medical Biobank




Phenotype

Tissue Blood

Biobank

Tissue Blood

Special diagnostics:• genome wide genotyping• genome wide expression analysis• metabolomic analysis from dried blood cards

Routine diagnostics

Routine diagnostics




Informed Consent

Liquid Biomaterial Tissue

Sample Preparation

Biobanking




1649

198

371

Liquid SamplesTissue Samples TumorTissue Samples Normal

196 Patients with liquid Biosamples

110 Patients with corresponding Tissue samples

57

3633

30

65

29

esophagus stomachcolon rectumovary pancreasother



500.000

Samples In Samples Out

690.000

83.300

38.800

> 60 Projects

Balance of biobanked Samples



Projects from LIFE and the LMBUnderstanding of Diseases• Interaction between diabetes and

allergy• Assessment of stress levels in saliva

and hair• Asthma and infection• Bone metabolism and growth• Iodine supply and thyroid function• Insulin secretion in oral glucose

tolerance test• brain serotyping• Ambrosia and Allergy• ApoE and Dementia• maternal nutrition and allergy

childhood development• Nutrition and Cognition• Sex hormones and brain structure• Adipozytokines and Diabetes• Plasticizers and childhood

development• …

Diagnostics and Therapy:• Diagnostics of cardiovascular diseases• Diagnostics of acute myocardial

infarction• Diagnostics of dementia• Diagnostics of depression• celiac disease screening• Reference values for steroids• Breast cancer and genetics• Lipid biomarkers and cognitive

performance• Biomarkers for the diagnosis of non-

alcoholic fatty liver disease in childrenand adolescents

• Intervention in the case of obesity• Biomarkers in Tumor Cachexia• Cutting cultures for investigating the

effect of chemotherapeutic agents• Tumor xenograft models for

investigation of the effect ofchemotherapeutics

• …



How to get our Samples and Data?

http://life.uni-leipzig.de/en/service/contact.html

• Be part of the University Leipzig or a collaboratingpartner (or contact us for advice)

• Inform about cohorts and data in the LIFE data portal(ldp.life.uni-leipzig.de)

• Write a project proposal and inform all respectivescientists and principle investigators about theproposal

• Present the proposal to the „Use and Access Committee“ (meets once per month)

• Contact LIFE-Data Management / Biobank after approval of the proposal to schedule logistics



Leipzig Medical Biobank as part of the German Biobanking Alliance (GBA)



Leipzig Medical Biobank as part of the German Biobanking Alliance (GBA)

GBANetwork of 11 BMBF-funded biobank sites Two IT expert centresInclusion of further biobanksCoordinated by the German Biobank Node (GBN -Representation of German biobanks in Europe (BBMRI-ERIC))

Leipzig Medical Biobank

harmonization and standardization of biobanking processes in different parts of the biobanking fieldmake biomaterials from various biobanks available for biomedical research



Work packages in GBA

WP5 Ethical, Legal

and SocialIssues (ELSI)

WP1 Central Executive

Management Office

WP2 The Biobanking IT

Network forGermany andBBMRI-ERIC

WP3 The Biobanking Quality System

WP4 Public Accountability,

Public Relations, Public Outreach

WP6 Counsellingbiobanks,

Education andTraining



Stakeholder Management in GBA

Technicalstaff

User: academia

User: industry

Donors/ patients

Potential user

Public/experts

Biobanks

Potential user survey

Workshopdonor

empowerment

User satisfaction

survey

GBN products & services survey

Technicians’educational

needssurvey

Workshops

StakeholderDialogue Forum I

User workshop: Evaluation ofIT search pilot

Qualitativeinterviews

StakeholderDialogue Forum II:

Industrycooperation



Summary

• The Leipzig Research Center for Civilisation Diseasescollected biosamples and data in a population based way.

• Within the last years the infrastructure of the LIFE-Biobankwas used to collect samples and data from cancer patients.The Leipzig Medical Biobank (LMB) was born.

• Samples and data from the Leipzig Medical Biobank wereused for different kinds of projects.

• The Leipzig Medical Biobank is part of the German BiobankAlliance reaching out to different Stakeholders in order toharmonize and standardize biobanking processes in differentparts of the biobanking field.

Thanks to:

LMB Team:Dr. Juliane DorowMadlen SiegemundAngelina MehrländerKristin KolbeBritt RosinRobin ScheunerSimone ScharfAlexander Kiel

Institute for Laboratory Medicine:Prof. Dr. Joachim ThieryProf. Dr. Uta Ceglarek

Cancer Center Leipzig:Prof. Dr. Florian LordickProf. Dr. Ulrich Hacker

Institute for Pathologie:Prof. Dr. Christian WittekindDr. Tina Unger

Surgery:Prof. Dr. Ines GockelDr. Orestis Lyros

LIFE Administration:Dr. Matthias Nüchter



Thank you for your attention!www.life.uni-leipzig.de

[email protected]

best practices forbiobanking –examples from the leipzig ......also hairat roomtemperature leipzig...

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