biochemistry resume 10

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Michael English 1425 Ryland Avenue Cincinnati Ohio, 45237 (513) 242-5909 E-Mail: [email protected] CAREER OBJECTIVE Seeking a full-time bioscience technician position utilizing detail oriented problem solving skills to provide a genuine service to the public with tangible results EDUCATION University of Cincinnati, Cincinnati OH GPA 3.0, Bachelor of Science, Bio-Chemistry April 27, 2016 University of Cincinnati Department of Chemistry, Cincinnati OH Vladislav Alexander Litosh Group, Nucleic Acid Modification May 11, 2015 August 8, 2015 Capstone Research; developed thymidine nucleic acid analogue leads to advance MCF-7 chemotherapeutic research. Developed passive targeting DNA polymerase terminating moieties, products were validated by H 1 NMR and IC 50 tested. WORK EXPERIENCE University of Cincinnati Department of Chemistry, Cincinnati OH Patrick Limback Group, Biological Mass Spectrometry 1 year of experience January 3, 2012 April 27 2013 Undergraduate work study: assisted graduate students with their research Protein and RNA assays Certified by University of Cincinnati Health and Safety Services RESEARCH EXPERIENCE Trained to perform routine protein and RNA assays Analytes were isolated by centrifugal separations Concentrations were measured by spectrometry Analytes were sequenced and assayed for functional activity Trained to modify thymidine to thymidine analogue by in vitro anabolic synthesis Hallmarks of MCF-7 were used to develop a motif that made DNA polymerase terminators more selective This motif was applied to thymidine to develop a lead for an effective DNA replication terminator Analogues were planned, synthesized, purified, verified by spectrometry, tested by IC 50 cell studies SKILLS Microscale Organic Chemistry: Recrystallization Extractions and separations Sequential Syntheses Ketone reduction using metal hydride reagent Photochemical isomerization of alkenes Esterification, Grignard, Wittig, Diels-Alder reactions E1 and E2 elimination reactions S N 1 and S N 2 nucleophilic substitution reactions Statistics: Hess’s Law Beer–Lambert law Spearman's rank correlation Standard score Mean, Median, Mode Null hypothesis significance testing Map making and spatial analysis C ++ programing 3D modeling and texturing

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Page 1: BioChemistry Resume 10

Michael English1425 Ryland Avenue

Cincinnati Ohio, 45237(513) 242-5909

E-Mail: [email protected]

CAREER OBJECTIVESeeking a full-time bioscience technician position utilizing detail oriented problem solving skills to provide a genuine service to the public with tangible results

EDUCATION University of Cincinnati, Cincinnati OH GPA 3.0, Bachelor of Science, Bio-Chemistry – April 27, 2016

University of Cincinnati Department of Chemistry, Cincinnati OH Vladislav Alexander Litosh Group, Nucleic Acid Modification → May 11, 2015 – August 8, 2015

Capstone Research; developed thymidine nucleic acid analogue leads to advance MCF-7 chemotherapeutic research. Developed passive targeting DNA polymerase terminating moieties, products were validated by H1NMR and IC50 tested.

WORK EXPERIENCEUniversity of Cincinnati Department of Chemistry, Cincinnati OH

Patrick Limback Group, Biological Mass Spectrometry → 1 year of experience January 3, 2012 – April 27 2013 Undergraduate work study: assisted graduate students with their research Protein and RNA assays Certified by University of Cincinnati Health and Safety Services

RESEARCH EXPERIENCE Trained to perform routine protein and RNA assays

Analytes were isolated by centrifugal separations Concentrations were measured by spectrometry Analytes were sequenced and assayed for functional activity

Trained to modify thymidine to thymidine analogue by in vitro anabolic synthesis Hallmarks of MCF-7 were used to develop a motif that made DNA polymerase terminators more selective This motif was applied to thymidine to develop a lead for an effective DNA replication terminator Analogues were planned, synthesized, purified, verified by spectrometry, tested by IC50 cell studies

SKILLSMicroscale Organic Chemistry: Recrystallization Extractions and separations Sequential Syntheses Ketone reduction using metal hydride reagent

Photochemical isomerization of alkenes Esterification, Grignard, Wittig, Diels-Alder reactions E1 and E2 elimination reactions SN1 and SN2 nucleophilic substitution reactions

Statistics: Hess’s Law Beer–Lambert law Spearman's rank correlation

Standard score Mean, Median, Mode Null hypothesis significance testing

Map making and spatial analysis C++ programing 3D modeling and texturing

Analysis: Adept in the science of analytical chemistry Skilled in compiling raw data objectively Interpreting data accurately, paying careful attention

to avoiding bias and logical error

Effective in using data assessment to gain understanding Presenting data accurately through equations, diagrams, charts,

symbols, maps and presentations Collaborates with scientific community

Equipment: Lab safety and maintenance Infrared Spectroscopy Hydrogen Spectroscopy Gravimetric and vacuum filtration

Hickman Still distillation Carbon Spectroscopy Nuclear Magnetic Resonance Spectroscopy Centrifuge separations

NanoDrop spectrometry Mel-Temp Apparatus Thin-Layer and Column Chromatography Mass spectrometry

Analytical Programs: Microsoft Excel NCSS Statistical Software Microsoft PowerPoint Microsoft Works Microsoft visual C++ 2010

Remote Sensing Environment for Visualizing Images (ENVI),

Environmental Systems Research Institute Arc View, Arc Map Surfer 8

SciFinder Map Viewer 5 Newtek Lightwave 3D 11 Basic Local Alignment Search Tool Adobe Photoshop CS6