Biocontrol

Post-harvest biofungicide

NEXY® is a unique post-harvest technology containing the naturally-occuring yeast Candida oleophila strain O to protect Citrus fruits, Bananas and Apples. NEXY® is a broad-spectrum biofungicide active against several pathogens as Botrytis cinerea, Penicillium spp, Colletotrichum musae.

• Active substance: Candida oleophila strain O is listed as an approved active substance in Europe (Regulation EC No 1107/2009 - Annex I)

• Composition: NEXY® is made of two components : - NEXY® Biomass: 3.1010 CFU/g of Candida oleophila- NEXY® Additive: Calcium gluconate

• Formulation: WG, wettable granule• Application: Post-harvest• Mode of application: Spraying, drenching, dipping, etc.• Maximum Residue Limit (MRL): Exempted from MRL in the EU, exempted from the requirement of a tolerance

for residues in the USA• Shelf life : 6 months at 20 °C / 18 months at 5°C

• Ability to grow at low temperature after application to ensure a long protection.- Multiplication of the yeast population by 1’000 in 2 weeks on

bananas at 13°C- Multiplication of the yeast population by 100 in 8 weeks on

mandarines at 4°C

• Stable in solution, the mixture can be kept during 2 days at 20°C.

Product Identity

NEXY® has a preventive action. Candida oleophila works through spatial and nutritive competition. The pathogen growth is reduced due to the lack of space and nutrients. Resources depletion stops pathogens growth.

Mode of Action

Technical features of Candida oleophila strain O

Population evolution of Candida oleophila strain O on bananas (13°C) and mandarines (4°C)

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Banana treated with NEXY® Mandarine treated with NEXY®

AGRAUXINE S.A. – Headquarter : 137 rue Gabriel Péri – 59700 Marcq-en-Baroeul – FranceOffice : 7, avenue du Grand Périgné - 49070 Beaucouzé - France – www.agrauxine.com

• NEXY® can be used in drenching, spraying, dipping, etc. The use of nebulization (cold fogging) is being investigated.• Rehydrate NEXY® biomass in water (20-25°C) during 30 min while agitating from time to time and then add NEXY®

additive and agitate continuously until total dissolution (15 to 30 min).• Maintain the mixture under agitation during the application.• NEXY® can be kept after opening in its own packaging during 14 days in cold condition (5-6° C), or 2 days at 25°C.• Compatibility:

- Compatible with sodium hypochloride (until 100 ppm)

Crop Usage Doses Registration in Registration pending in

Apples & Pears Botrytis cinerea, Penicillium expansum, Penicillium spp

33g of NEXY® biomass and 200g of NEXY® additive for 100 L of

mixture

France, United-Kingdom, Netherlands, Austria

USA : EPA level

Germany, Poland, Spain, Greece, Italy

Bananas Colletotrichum musae France (West Indies) Spain,1

CitrusPenicillium spp,

Penicillium italicum, Penicillium digitatum

France Spain, Italy, Greece

1 Other countries (Latin America, Central America & Africa from 2018)

Usages and doses

Application advices

• One pack containing 33g of NEXY® biomass and 200g of NEXY® additive• Other packagings: on request

Packaging

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Trialn°1- 101dayscoldstorage Trialn°2- 57dayscoldstorage

Control

NEXY

Reference

Apples – Botrytis cinerea% infected fruits – GEP trialsItaly 2004 (trial n°1) – Belgium 2004 (trial n°2)

01020304050607080

Trialn°3- 42dayscoldstorage Trialn°4- 63dayscoldstorage

Control

NEXY

Reference

Apples – Penicillium expansum% infected fruits - GEP trialsPoland 2012 (trial n°3) – Italy 2004 (trial n°4)

0102030405060708090100

Trialn°5- 45dayscoldstorage Trialn°6- 45dayscoldstorage

Control

NEXY

Reference

Citrus – Penicillium spp.% infected fruits - GEP trialsSpain 2012 (trial n°5) – Spain 2013 (trial n°6)

0102030405060708090

100

Trialn°7- 12daysat13°CTrialn°8- 12daysat13°CTrialn°9- 15daysat13°C

Control

NEXY

Reference

Bananas – Colletotrichum musae% infected fruits - GEP trialsSpain 2013 (trial n°7 & 8) – Spain 2014 (trial n°9)

Apples – Botrytis cinerea% infected fruits – GEP trialsItaly 2004 (trial n°1) – Belgium 2004 (trial n°2)

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Trialn°1- 101dayscoldstorage Trialn°2- 57dayscoldstorage

Control

NEXY

Reference

Apples – Botrytis cinerea% infected fruits – GEP trialsItaly 2004 (trial n°1) – Belgium 2004 (trial n°2)

01020304050607080

Trialn°3- 42dayscoldstorage Trialn°4- 63dayscoldstorage

Control

NEXY

Reference

Apples – Penicillium expansum% infected fruits - GEP trialsPoland 2012 (trial n°3) – Italy 2004 (trial n°4)

0102030405060708090100

Trialn°5- 45dayscoldstorage Trialn°6- 45dayscoldstorage

Control

NEXY

Reference

Citrus – Penicillium spp.% infected fruits - GEP trialsSpain 2012 (trial n°5) – Spain 2013 (trial n°6)

0102030405060708090

100

Trialn°7- 12daysat13°CTrialn°8- 12daysat13°CTrialn°9- 15daysat13°C

Control

NEXY

Reference

Bananas – Colletotrichum musae% infected fruits - GEP trialsSpain 2013 (trial n°7 & 8) – Spain 2014 (trial n°9)

Oranges – Penicillium spp.% infected fruits - GEP trialsSpain 2012 (trial n°5) – Spain 2013 (trial n°6)

Apples – Penicillium expansum

Bananas – Colletotrichum musae% infected fruits - GEP trials - French West Indies (4 trials) – Focus on 11-12 days assessments

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Note : All trials are GEP trials (with artificial inoculation)

NEXY® used on apples, bananas, citrus fruits shows an efficacy comparable to the chemical references. NEXY® is a good alternative to chemical fungicides. In addition, biological control of post-harvest diseases is of uppermost interest comparatively to chemical fungicides: absence of residues in the food chain and low risk of appearance of fungicide resistant pathogens.

Trial Results

Treated with NEXY® Non-treated

AGRAUXINE S.A. – Headquarter : 137 rue Gabriel Péri – 59700 Marcq-en-Baroeul – FranceOffice : 7, avenue du Grand Périgné - 49070 Beaucouzé - France – www.agrauxine.com