bioengineering the future with shell oil. transport fuels 75% of liquid fuels are used for...
TRANSCRIPT
Transport Fuels
75% of liquid fuels are used for transport. 70% of that in cars.
Retail fuel is 95% fossil alkanes and 5% from a biological source.
By 2020, global demand for bio-derived fuels will be 500B litres.
Wood Image
The Exeter-Shell Projects
Genes for Alkane Production inHigher Plants
Inducible Wax SynthesisIn Salt Cress
Development of High-throughputMutant Analysis and Wax Imaging
Molecular Control of HCProduction & Secretion in Botryococcus braunii
B. braunii-BacterialInteractions
B. Braunii GenomeEvolution
Botryococcus braunii Culture SystemsProductivity & HC Extraction
Synthetic Ecologyfor Optimum Algal Growth
HC Synthesis Screen in Oleaginous Yeasts
Rhodosporidium toruloidesGenome Analysis
High-throughputScreen Development
Alkane Production & Secretionin Vibrio furnissii
Alkane Synthesis incyanobacteria
Alkane Synthesis inDesulfovibrio
FA Catabolism incyanobacteria
Alkane Synthesis inMycobacterium
Forced Evolution& Mutagenesis for
Increased FA
Bacterial Population Samplingfor HC Production
Molecular & HC AnalysisOf Isolated Mutants
Molecular Control of Hydrocarbon Synthesis & Secretion in Bacteria, Yeast and Algae
for Advanced Biofuel Production
Metabolism &Enzymes
Gene & PathwayDiscovery
MolecularBioinformatics
Synthetic Biology
HC Analysis
Culture Systems& DSP
C-Flux EngineeringIn YeastVariable Chain Length & Branched HC
in Fermentative Microbes
Synthetic Biology
Engineer the production of
gasoline, jet-fuel, diesel and
lubricant oil in E. coli that are
identical to fossil fuels.
Biological Alkanes require
no alteration of current
engine technologies.
acoL
bkdAA &
bkdAB
bkdB
ilvE
fabHB
accA to accD
ILV Keto acid
MDHLA
Keto acyl CoA
Malonyl-ACP
Acetyl CoA
Fatty acyl-ACP
Branched fatty acyl-
ACP
fabH
FFA
F-al
Alkanes &
alkenes
NpAD
luxC & luxE
tesA
FATB1
luxD
FATB1
luxD
“Third Generation” Biofuels from Algae
Renewable.
Sustainable.
Productive.
CO2 Mitigation.
Experimental.
Botryococcus braunii Oils.
Synthesises and secretes very long chain hydrocarbons.
Major fossil component of oil wells.