BioinformaticsBioinformaticsNavigating the Web Accessible Navigating the Web Accessible

DataDataPlant Root Genomics ProjectPlant Root Genomics Project

MUPGRET Weekend Workshop MUPGRET Weekend Workshop

Root Genomics Mini ConferenceRoot Genomics Mini Conference

Dr. Gordon K. SpringerDr. Gordon K. Springer

February 20, 2005February 20, 2005

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tlclustercluster over library

tlclustercluster over plate

PHREDquality

assessment

ValSeqValidate Phred

results& identifymarkers

CrossmatchEliminate vector

UpdateStatistics

for plate

UpdateStatistics

for library & project

Plate availablefor annotationand database

searching.

Zip FileValidation

Trimming

Keck to UMC

EST Sequence PipelineEST Sequence Pipeline

GBsubmitPrepare data forGB submission

NCBIGenBank

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Plant Root Genomics HomePlant Root Genomics Home

Direct AccessDirect Access

http://genome.rnet.missouri.edu/http://genome.rnet.missouri.edu/RootsRoots

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Root Data AccessRoot Data Access

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Getting Started from MenuGetting Started from Menu

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Project EST StatisticsProject EST Statistics

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Library StatisticsLibrary Statistics

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Look at a Plate of DataLook at a Plate of Data

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Pick a SequencePick a Sequence

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Looking at an EST Looking at an EST SequenceSequence

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See Sequence in GenBankSee Sequence in GenBank

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Back to EST SequenceBack to EST Sequence

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See Similar Sequences at See Similar Sequences at TIGRTIGR

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Back to EST SequenceBack to EST Sequence

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Best Blast “Hits” in Best Blast “Hits” in GenBankGenBank

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Back to EST SequenceBack to EST Sequence

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Do a Blast AnalysisDo a Blast Analysis

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Blast Results from ZMRABlast Results from ZMRA

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Clustering DataClustering Data

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Identifying UnigenesIdentifying Unigenes

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Project TableProject Table

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Live DemoLive Demo

http://genome.rnet.missouri.edu/Rootshttp://genome.rnet.missouri.edu/Roots

For Further Information:For Further Information:

Rootgenomics Home PageRootgenomics Home Page

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Team MembersTeam Members

Team LeadTeam LeadGordon K. SpringerGordon K. Springer**

Post-docPost-docWilliam G. SpollenWilliam G. Spollen**

IATS/RSCIATS/RSCLarry SandersLarry SandersDenis HancockDenis HancockMike WoodsonMike Woodson

StaffStaffHector Sanchez-VilledaHector Sanchez-Villeda**

Steve SchroederSteve Schroeder**

UMC Graduate StudentsUMC Graduate StudentsChris TopinkaChris TopinkaShafquat BhuiyanShafquat BhuiyanJay HutchinsonJay HutchinsonAbdul KhambatiAbdul KhambatiRahul PatelRahul PatelJim RiesJim Ries**

Arturo GuillenArturo Guillen**

http://beagle.rnet.missouri.edu/Biogroup

*Root Project *Alumni

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Stay TunedStay TunedThe Story Continues …The Story Continues …

Questions?Questions?