biological engineering education and computational methods...biological engineering education and...
TRANSCRIPT
Biological Engineering Education and Computational Methods
Effect of the Geometrical Structure of Mitochondria on the Synthesis and Diffusion of Oxidative Metabolites
Rajan Gangadharan1 and Dr.Guigen Zhang1,2,3
1Micro/Nano Bioengineering Laboratory, 2Nanoscale Science and Engineering Center, 3Faculty of Engineering, The University of Georgia, Athens, GA 30602
Introduction
• Abundant new information on the structure and dynamics of living cells are currently available
– Aided by advances in Microscopy & Molecular biology.
• Computational approaches are needed to link all these new data
• Issues in computational approach: Cell systems are complex
– Interdependent chemical reactions and structural components regulates cell processes
Introduction
• Spatiotemporal computational approaches ‐one way to answer the complexity
• Partial differential equations ‐ defines concentration changes in space and time.
– ODEs can define reactions, membrane transport and electrical potential
– Diffusion of molecules within complex geometry defined by using PDEs
Research background – Mitochondria cristae structure
Research Questions
Previous Work
Model Development
Model Development
Model Geometry
Model Development
Model Development
Example: For complex I the reaction rate is defined as
Results – Effect of the tubular junction on ADP concentration
Results – Effect on membrane potential
Effect of diameter of tubular connection on potential
Time (s)
10-1210-1110-1010-9 10-8 10-7 10-6 10-5 10-4 10-3 10-2 10-1 100 101 102 103 104M
embr
ane
Pot
entia
l (m
V)
150
160
170
180
190
200
No tubular connectionTubular connection - 40nm diaTubular connection - 20 nm dia
Potential change at various points on the inner membrane and cristae
Time (s)
10-1210-1110-1010-9 10-8 10-7 10-6 10-5 10-4 10-3 10-2 10-1 100 101 102 103 104
Mem
bran
e P
oten
tial (
mV
)
155
160
165
170
175
180
185
190
195Point APoint BPoint CPoint D
Conclusion and Further Work
I thank Dr. Guigen Zhang, My group members and Faculty of Engineering ,
University of Georgia for their support
Thank You