biology 2a03 - lab 1 outline 2012

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  • 8/2/2019 Biology 2a03 - Lab 1 Outline 2012

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

    This lab chapter was developed by and is the property of the Biology Department, McMaster University. Unauthorized copying of this laboratory

    chapter in its entirety or part is prohibited without permission of the Biology Department, McMaster University

    BIOLOGY 2A03 LAB 1

    HUMAN NUTRITION TOTAL BODY ENERGY BALANCE

    Note: This is a semi (or partial)-formal lab report, due ONE weekfrom the date of your lab

    1 (January 23 27, ALL lab sections, ODD & EVEN). Lab reports are due by 5 pm on their

    due date. Hand your lab report to the appropriate drop box on the first floor of Life Sciences

    (beside the elevators). It is your responsibility to ensure that your lab report is complete,

    handed in on time and placed in the correct drop box. Late lab reports will not be marked andwill receive a grade of zero. If you miss attending lab 1, this lab report is still due on the

    due date. A MSAF or a note from the deans office may give you an extension on the

    due date for lab 1 but does not excuse you from handing in this lab report.

    Check list of materials to bring to lab 1:

    Three days worth of Kilocalorie uptake charts completed (charts on pages 12 14)(Pre-lab assignment)

    One day Kilocalorie expenditure chart completed (chart on page 15) (Pre-labassignment)

    Calculation of your personal BMR (pages 5-7) (Pre-lab Assignment).

    Calculator for data calculations or

    Laptop to record data1

    Pens, pencil, eraser

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

    This lab chapter was developed by and is the property of the Biology Department, McMaster University. Unauthorized copying of this laboratory

    chapter in its entirety or part is prohibited without permission of the Biology Department, McMaster University

    Objectives: To evaluate the adequacy (or inadequacy!) of your diet by careful

    monitoring of daily food consumption.

    Reading from textbook that might be helpful:Note: Ch. 21: pages 606-615 (3rd edition), pages 604613 (4th edition)

    Total body energy balance means that the total energy expenditures of the body, whether byexternal work, energy storage, or production of internal heat, must be equal to the total bodyenergy intake in the form of food.

    ENERGY INTAKE = INTERNAL HEAT + ENERGY STORAGE(From food intake) PRODUCED

    Most of us actually eat a reasonable healthy diet by a combination of conscious motivationand subconscious physiological controls. A few of us eat a nutritionally poor diet and pay theconsequences. In this lab, you will survey your own diet and compare it to bothrecommended standards and your own estimated energy expenditure as they are subject to

    many inaccuracies due to the need for estimating or guesstimating. Furthermore, you willonly monitor total kilocalorie and protein intake. It is possible that a diet adequate in proteinsand kilocalories may be inadequate in minerals or vitamins; however this is highly unlikelyunless your diet is unusual (e.g., all your protein comes from vegetables, or all your caloriescome from beer!).

    Pre-lab assignment, record on pages 12 14), complete before lab 1

    tutorial, January 16-20 (all lab sections)Your TA will initial your pre-lab assignment to acknowledge that it was done. Completion ofthe pre-lab assignment is worth 5 marks.

    A) 3 day diary of your personal caloric intake (recorded on pages 12 - 14)B) 1 day diary of your personal energy expenditure (recorded on page 15)C) Calculation of your BMR (see pages 5 7 & recorded on page 16)

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

    This lab chapter was developed by and is the property of the Biology Department, McMaster University. Unauthorized copying of this laboratory

    chapter in its entirety or part is prohibited without permission of the Biology Department, McMaster University

    A) CALORIC INTAKE

    Note: Be careful in your use of the term calorie. A calorie is the amount of heatneeded to raise the temperature of 1g of H2O by 1 C. The energy content offood molecules is generally expressed in the kilocalories (i.e., 1000 calorieunits). However, nutritionists use the term Calorie to mean kilocalorie. Calorie,written with a capital C means 1000 calories or 1 kilocalorie.

    1. For a period of three days, keep a record of all the foods you eat, both by type and byamount using the forms provided. Try to eat a normal diet (i.e., whatever you would

    normally eat; dont modify your habits because of this survey). In the accompanyingtables (posted as a separate pdf file on Avenue 2 Learn), the food energy(kilocalories) and protein content of most common foods are listed. Two useful websites with more detailed information, especially for unusual foods is:

    http://www.nal.usda.gov/fnic/foodcomp/search/ (USDA)

    http://www.hc-sc.gc.ca/fn-an/alt_formats/hpfb-dgpsa/pdf/nutrition/nvscf-vnqau-eng.pdf (Health Canada)

    Nutrition Facts Table (how to read a food label):http://hc-sc.gc.ca/fn-an/label-etiquet/nutrition/cons/index-eng.php or

    http://www.hc-sc.gc.ca/fn-an/label-etiquet/nutrition/cons/inl_flash-eng.php

    Note: You do not need to download and print documents from these web sites in their

    entirety. You can access the information you need online.

    The measures or portions are largely listed in the tables, in units of cups or oz. These willbe demonstrated in the lab for those unfamiliar with these units. Using these units, try toguesstimate your portions as accurately as possible. Of course, not all foods will be listedin the tables, and for these you will just have to use your common sensed to choose the bestequivalents for purposes of calculation.

    2. From the record compiled in the charts, calculate your average daily intake of totalkilocalories and of protein alone.

    The National Research Council recommends that a healthy 25 year-old male ofaverage build and weighing 65 kg should have a daily intake of 3200 kilocalories.Similarly a healthy 25 year-old female of average build and weighing 55kg should

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

    This lab chapter was developed by and is the property of the Biology Department, McMaster University. Unauthorized copying of this laboratory

    chapter in its entirety or part is prohibited without permission of the Biology Department, McMaster University

    Apply your weight to the appropriate graph (SEE SUBSEQUENT PAGE), to determinewhat your caloric intake should be (1 lb = 0.4536 kg). The amount of total intakewhich should be from protein is at least 1g/kg body weight.

    How much of our daily caloric intake comes from proteins? The caloricequivalent of 1 gm protein is 4.0 kilocalories. (Note: This figure takes into account thefact that protein is not fully oxidized in metabolism and that absorption of protein is not100%).

    All these figures are for a fairly active person and would have to be adjustedupwards or downwards for very active and inactive people. Section B will relate youractivity level to caloric intake.

    You must have the three daily calorie intake & calorie expenditurecharts completed and calculated your personal BMR value before

    your first lab class, January 16 20, 2012.

    B) ENERGY EXPENDITURES

    Keep a record of your activities for 1 of the 3 days (we only ask for 1 day here as itcan be very tedious). Was this typical of the three? If not, adjust the figures to berepresentative of a typical day.

    Sample recordSleeping 8.50 hAwake, lying still 0.75 hDressing 0.25 hDriving 1.00 hTaking notes 4.25 hWalking slowly 1.75 h

    Eating 1.50 hLab Work 2.00 hSwimming 1.00 hStanding 1.50 hTyping 1.25 hRunning 0.25 h

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

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    chapter in its entirety or part is prohibited without permission of the Biology Department, McMaster University

    Individuals performing the same activities do not necessarily expend the same amountof energy in that performance. Caloric expenditure during any activity varies as amultiple of the basal metabolism of the individual, which in turn varies with bodysurface area as determined by the height, weight and sex of that individual.

    The Basal Metabolic Rate (BMR) may be defined as the energy required to maintainbody functions while awake, but at absolute rest, and after a period of fasting longenough to avoid the specific dynamic action of food intake on metabolism.

    BMR may be obtained clinically by the measurement of O2 consumption following anight of restful sleep and a 12-hour period of fasting. (Note: In a later lab (lab 5), you

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

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    For every 1 litre of O2 consumed, approximately 4.8 kilocalories of stored chemical

    energy are liberated from the molecules being oxidized).

    A reasonable estimate, however, of BMR can be obtained from a simple calculationbased on age, height and weight using the tables provided. Record the mostappropriate basal metabolism value from the table below for your age and sex.

    A = kilocalories/square metre/hour

    Basal Metabolism Values (kilocalories/m

    2

    /hr)Age Males Females18 40.8 35.819 40.5 35.420 39.9 35.321 39.5 35.222 39.2 35.2

    23 39.0 35.224 38.7 35.125 38.4 35.126 38.2 35.027 38.0 35.0

    Note: If your particular age lies outside this table, consult the Figure below.

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

    This lab chapter was developed by and is the property of the Biology Department, McMaster University. Unauthorized copying of this laboratory

    chapter in its entirety or part is prohibited without permission of the Biology Department, McMaster University

    Record your body surface area according to your weight and height from the chart

    below. A ruler placed on your height in the left hand column and on your weight in theright hand column will pass through the correct surface area in the middle column B =

    m2

    Multiply: A X B to find the basal metabolic rate for you.

    A X B = kilocalories/hour (BMR)

    By applying your BMR to each of the appropriate values in energy expenditure table,you will determine the energy you expend in kilocalories/hour over your 24 hour day.

    For example: The basal metabolism value of a 20 year old male is 39.9 kCal/m2/hr(A). If he was 65 tall and weighed 163 lbs., his body surface area would be 1.7square meters (B). His BMR would be 39.9 X 1.7 = 67.8kCal/hr.

    NOMOGRAM FOR THE CALCULATION OF BODY SURFACE AREA

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

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    While washing the dishes for 15 minutes, the energy he would expend would be:

    67.8 X 2.2* X 0.25** = 37.3 kcal

    *see table of activities on the next page for source** one quarter of one hour

    Calculate from the table on the following page (use common sense and guesstimatefor unlisted items) what your energy expenditure is in kilocalories for each of theactivity periods. Sum the figures to find the average daily energy expenditure.

    Light Activities B.M.R. MultiplierSleep 1.0 x B.M.R.Awake, lying still 1.2 x B.M.R.Sitting

    at rest

    and reading

    and writing or taking notes,homework

    and eating

    and typing (rapidly)

    1.4 x B.M.R.1.7 x B.M.R.2.1 x B.M.R.2.2 x B.M.R.2.3 x B.M.R.

    Standing relaxed 1.6 x B.M.R.Dressing and undressing 1.8 x B.M.R.Driving an automobile 2.0 x B.M.R.Dishwashing, ironing, etc. 2.2 x B.M.R.Playing cards 2.6 x B.M.R.Walking slowly 3.0 x B.M.R.Bicycling (for fun) 3.1 x B.M.R.Light exercising 3.2 x B.M.R.

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

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    Moderate Activities B.M.R. MULTIPLIERVacuuming, sweeping or cleaning house 4.0 x B.M.R.Walking fast 4.3 x B.M.R.

    Dancing (moderate speed) 4.6 x B.M.R.Walking downstairs 0.02 Cal/kg/15 stepsModerate exercising 5.5 x B.M.R.Laboratory work 6.0 x B.M.R.

    Cleaning windows 6.0 x B.M.R.

    Horseback riding moderate speed 7.2 x B.M.R.

    Swimming - recreational 7.5 x B.M.R.Sawing wood 7.8 x B.M.R.Skating - recreational 8.0 x B.M.R.

    Bicycling moderate 8.0 x B.M.R.

    Jogging 8.6 x B.M.R.

    Shoveling snow 8.6 x B.M.R.

    Heavy Activities B.MR. MULTIPLIERRowing 12.7 x B.M.R.Walking upstairs 0.07 Cal/kg/15 stepsDancing fast 15.0 x B.M.R.

    Playing ping-pong seriously 17.5 x B.M.R

    Skating speed skating 18.0 x B.M.R.Gardening digging 19.8 x B.M.R.Bicycling - racing 20.0 x B.M.R.Skiing 20.0 x B.M.R.Horseback riding hard 22.0 x B.M.R.

    Swimming racing 25.0 x B.M.R.Exercising heavy 30.0 x B.M.R.Fencing 37.0 x B.M.R.

    Football 43.8 x B.M.R.Boxing 45.0 x B.M.R.

    In your Results you will be reporting on your daily average intake and expenditure.+/- 10% of total kilocalories/day is probably an insignificant deviation from balance given theinaccuracies in the methods If you are further than 10% of total kilocalories/day from

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

    This lab chapter was developed by and is the property of the Biology Department, McMaster University. Unauthorized copying of this laboratory

    chapter in its entirety or part is prohibited without permission of the Biology Department, McMaster University

    the weight gain or loss is in the form of fat (adipose tissue), a reasonable conversion factor is

    8 kilocalories/gm of tissue (the caloric value of pure fat is about - 9.4 kilocalories/gm butadipose tissue contains approximately 10% water).

    Pre-Lab Assignment: To be completed before the start of the lab, January 16 20. YourTA will initial your pre-lab assignment to acknowledge that it was done. Completion of thepre-lab assignment is worth 5 marks.

    1. Before your first lab you will keep your 3 day food diary or kilocalorie intake charts and

    one day exercise or kilocalorie expenditure chart (see the following pages). You need notfully complete the chart in their entirety. What must be completed on each chart is:

    Kilocalorie Intake charts (3): A list of all foods consumed over a three day a period An estimation of the quantity i.e. 1 cup, 8 ounces, 250 ml, 100 gm, 1b, 1 unit, 6

    pieces, etc

    For example a one day food diary may contain the following entries:

    Breakfast: 1 Tim Hortons breakfast wrap with bacon 1 unit1 XL Tim Hortons tea, 1 milk & 1 sugar

    Mid-morning snack 2 small mandarin oranges100 ml yoghurt1 XL tea, 1 milk, 1 sugar

    Lunch 1 Wendys Mandarin Chicken Salad1 diet cola

    Mid afternoon 1 XL tea, 1 milk, 1 sugar

    Supper Meat loaf, 2 slices, approx. lb

    250 ml mashed potatoes cup gravysteamed peas, cup350 ml diet cola

    Although the chart requires you to calculate other factors (i.e. protein, kilocalories,protein kilocalories), it is not necessary for you to complete these calculations before

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    the lab class. Your TA will help you to complete these calculations during the lab

    tutorial.

    2. Exercise Kilocalorie expenditure chart:

    On a typical day (for you), you will keep an exercise kilocalorie expenditure diary andrecord your activities in the chart provided. Before your first lab you must have thefollowing information recorded in your chart:

    Type of activity (i.e. sitting, biking, swimming, sleeping, walking, etc.) How you would classify it (i.e. light, moderate or heavy) Duration of the activity This chart must be completed (with your personal BMR value & BMR

    multiplier values for all activities) before the end of the Lab 1 tutorial

    3. You will also calculate your personal BMR value and make note of it on theExercise kilocalorie Activity chart (p. 15). If you have trouble calculating your

    BMR, your TA can assist you during the Lab 1 tutorial but you must haveyour BMR value calculated by the end of the Lab 1 tutorial. You must alsomake not of your BMR as this value is required for Lab 5 later on in the term.You will need it when you answer the theory questions for lab 5. Perhaps youcan make a note of it in your computers calendar to pop up on the date ofyour Biology 2A03 lab 5.

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    TA Initials:

    Table A1a:KILOCALORIE INTAKE CHARTDate: Include this chart in your Appendix

    DAY 1 Date:

    Type ofFood

    Measure i.e.volume orImperial

    units

    Quantity(g)

    Protein(g)

    Totalkilocalories

    Proteinkilocalories

    Total Caloric Intake =

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    TA Initials:

    Table A1b: KILOCALORIE INTAKE CHARTDate: Include this chart in your AppendixDAY 2- Date:

    Type ofFood

    Measure i.e.volume orImperial

    units

    Quantity(g)

    Protein(g)

    Totalkilocalories

    Proteinkilocalories

    Total Caloric Intake =

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    TA Initials:

    Table A1c: KILOCALORIE INTAKE CHARTDate: Include this chart in your AppendixDAY 3- Date:

    Type ofFood

    Measure i.e.volume orImperial

    units

    Quantity(g)

    Protein(g)

    Totalkilocalories

    Proteinkilocalories

    Total Caloric Intake =

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    TA Initials:

    Table A2: KILOCALORIE EXPEDITURE CHART Include this chart in your AppendixDate: ONE TYPICAL DAY

    ActivityLevel ofActivity

    LightULevel of

    Activity Moderate U

    Level ofActivity Heavy U

    TimeActivity

    Sustained(minutes)

    BMRMultiplier

    Kilocaloriesexpended*

    Total Kilocalorie Expenditure =*Your BMR X the BMR Multiplier (for that activity) X time (minutes) = kilocalories expendedSee pages 5-8 for a sample equation.

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

    This lab chapter was developed by and is the property of the Biology Department, McMaster University. Unauthorized copying of this laboratory

    chapter in its entirety or part is prohibited without permission of the Biology Department, McMaster University

    In this lab report you are going to be guided in writing up a formal Results section. You will

    answer some theory questions (pages 19 21) but they will NOT be written up as a formallab report Discussion section. You will write a half page Conclusion to this exercise. See thecheck list at the end of lab for what to include in your partial lab report. Your lab report willbe due one week after you complete the lab. Your TA will endeavor to return your labreport to you before your first formal lab report is due so you have feedback beforeyou write a formal lab report.

    RESULTS (Presentation of data) vs. APPENDIX

    A Results section must start off with some text. You never begin the results section with aTable or a Figure (graph).Students are often confused about what type of data to present in the Results section andwhat to present in the Appendix. Basically, you present summaries of data in the Resultssection. Only what the reader really needs to know goes into the Results. Individual results,raw data, calculations all go into the Appendix.Therefore for Lab 1, In your Appendix, include your 4 handwritten raw data sheets (with your

    TAs initials).Create a table that summarizes your raw daily diet & energy expenditure data (Table A1)Example:Table A4: Summary of nutrition data for 20 year old female subject (not the studentsname).

    Food category Day 1 Day 2 Day 3 Three day average

    Total kilocalories consumed

    Total protein kilocaloriesconsumed

    Subject BMR (kilocalories/square metre/hour)

    Subject Total Kilocalorie Expenditure per day

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

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    In the Results section You need a table that summarizes the data in Table A1 i.e.

    Table 1: Analysis of the diet of a 20 year old female subject over a 3 day period.

    Average Total

    kilocalories

    consumption

    Average Protein

    consumption per

    day

    Student subject

    Recommended kilocalories per day(give your reference)

    Diet Imbalance? +/-?

    Total kilocalories per day expended

    through activity

    Diet/Activity Imbalance? +/-?

    Put the results of calculations in your table. Do not put the details of your calculations inthe Results section. The details of calculations go in your Appendix.

    Points to remember when inserting a table in your lab report: Table summarizes yourdata in Lab 1 = 10 marks Title: Table 1: (2 marks) Header row and column titles with units specified. E.g. temperature oC, or

    pH, time measured in minutes or seconds (4 marks) Appropriate data i.e. summaries (e.g. means, medians, maximum, minimum,

    standard deviation values of data but NOT entire set of data) (4 marks)

    Graphing your results: Graph summarizing your data in lab 1 = 10 marks

    The data in this lab is not suitable for a linear graph as you are not analyzing a change overthe 3 day period. You are analyzing the average over a three day period. Therefore you willpresent your data in a bar graph form in addition to having it in a table.

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

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    For a summary graph of Table 1:

    Title = 2 marks Figure # & descriptive title. The title for graphs (figures) goesat the bottom of the figure. If you have created a graph using Excel, you willhave to delete the title that Excel imbeds in the graph as it will be at the top ofthe graph.

    X + Y axis word titles with units = 3 marks

    Appropriate type of graph (bar vs. linear) for the type of data = 2 marks

    Appropriate bar or line produced for the graph and easy to differentiatebetween different bars or lines (either by colour or shading as above) (3marks)

    Marks will be deducted if the graph background is shaded, or the graph issurrounded by a border.

    Energy expenditure should fit on your graph with the diet summaries.

    Note: Normally you would NOT present the same data in both a table and a figure.

    You would choose which format best presents the data and only use one. However for

    teaching purposes, you are required to present your data in a table and a figure for

    this lab report.

    Text presentation of the results with no interpretation = 20 marks

    Once you have prepared your tables, graphs & done your calculations you must write

    a text presentation for your Results section. Your text presentation should have

    - no personal pronouns (i.e. no me, my, I but students should refer to themselvesas for example the 20 year old female or male subject

    - be in the past tense (do not mix tenses)- proper use of abbreviations & define an abbreviation the first time it is used

    -type a space between a digit an a unit eg. 1 mm

    - Should start results section with text, not a figure or table

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

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    CONCLUSIONS Questions to answer in your conclusion section:

    Is your diet adequate in terms of both calories and protein? In not, should you adjust youractivity level of your diet or compensate? Will you do it? If you dont, what do you predictwill be the long term physiological consequences? (5 MARKS)

    Appendix (raw data tables, tables and/or graphs that present data before it is summarized,any calculations, pre-lab assignment pages (12-16))

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    PAGE TOTAL = /10

    Theory Questions of (Name & ID):

    Lab Section:Marks

    List two factors physiological factors which might be responsible for differences inBMR between individuals and which may be controlled by the individual: (1 markfor each factor listed & 2 marks for each explanation of the factor)

    A.

    B.

    /6

    Why do the basal metabolism values (see the table & figure on page 6) changewith age?

    /1

    +/- 10% of total kilocalories/day is probably an insignificant deviation from balance given theinaccuracies in the methods. If you are further than 10% of total kilocalories/day from balance,translate the imbalance into a rate of weight gain or weight loss. If we assume that the weight

    gain or loss is in the form of fat (adipose tissue), a reasonable conversion factor is 8kilocalories/gm of tissue (the caloric value of pure fat is about-9.4 kilocalories/gm but adiposetissue contains approximately 10% water)

    PAGE TOTAL = /7

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    Theory Questions of (Name & ID):

    Lab Section:Marks

    How does your average daily expenditure compare with your average dailyenergy intake calculated in section A2? Are you in energy balance?

    2

    Discuss any other factors which must be taken into account to construct a moreaccurate energy balance sheet. In particular, indicate two sources of errorsthat might occur in the assumptions we have made so far and discuss howa more accurate energy sheet might be created. Each factor = 1 mark,discussion of how to correct the source of error = 1 mark.

    A.

    B.

    4

    PAGE TOTAL = /6

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    Theory Questions of (Name & ID):

    Lab Section:Marks

    Notice that at several points in the calculations the expectation we have forfemales are different from those we have for males. This is not a sexist attitude.Discuss reasons for this briefly, in physiological terms. Give three physiologicalreasons for the difference between the sexes and briefly explain each inphysiological terms. Each reason = 1 mark, explanation = 1mark.

    A.

    B.

    C.

    6

    What happens to proteins (amino acids) when you are in an anabolic versus acatabolic state?

    5

    PAGE TOTAL = /11

    Total of theory questions = /34 marks

    References = /6 marks

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

    This lab chapter was developed by and is the property of the Biology Department, McMaster University. Unauthorized copying of this laboratory

    chapter in its entirety or part is prohibited without permission of the Biology Department, McMaster University

    References for theory questions (list three from peer reviewed scientific journalsdated from 2012 back to 2007: (6 marks)

  • 8/2/2019 Biology 2a03 - Lab 1 Outline 2012

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

    This lab chapter was developed by and is the property of the Biology Department, McMaster University. Unauthorized copying of this laboratory

    chapter in its entirety or part is prohibited without permission of the Biology Department, McMaster University

    PARTIAL OR SEMI-FORMAL LAB REPORT:

    Lab 1 is a partial or semi-formal lab report. It consists of some but NOT ALL of the featuresof a formal lab report. You are to follow the style rules of the Journal of ExperimentalBiology. The rule for authors submitting articles to this journal have been posted online foryou. You can also check the rules at:http://www.biologists.com/web/submissions/jeb_information.html .Many of the instructions will not apply but pay particular attention to the instructions for:Text, Appendices, References in text, Reference list, preparing the figures, graphs andline art, text labeling. If you have questions you may find it useful to browse through an

    issue of this journal to see how tables, graphs and references are done.

    Page limit rules: Font Helvetica or Arial, size 12, 1.5 spacing for all text.Margins = 50 pt top, bottom, and right, left.Page limits are set for each section of your lab report. Any text that exceeds the page limitwill not be marked by your TA. You cannot save space in one section (e.g. Results) anduse it for Discussion. Printing double sided is a great idea to save paper but you cannotexceed the page limit by handing in 2 pages of double sided text and have it count as 2pages of single sided. Many students find keeping to the page limit difficult but it can bedone. Writing scientific papers requires you to be brief and to the point. Scientific journals,grant applications and bids for contractual work all require the applicants to keep to page andword limits.

    It is YOUR responsibility to make sure that your report is complete and in the correct order. Itis strongly suggested that you number your pages. This will help you to ensure that you haveput your lab report in the correct order.

    Check list for lab 1:

    Cover or Title PageResults (including text explaining the

    results, tables & graphs thatsummarize the results, results ofcalculation, usually NO calculations gohere)

    Conclusion

    Appendix (raw data tables, tablesand/or graphs that present databefore it is summarized, anycalculations pre-lab assignment

    Marking rubric (pp. 25 - 26)

    Theory Assignment, Pages 19-22with References(as a separateassignment)

    The partial lab report = 60

    Theory assignment (pp. 19-22) = 40

    Total = 100

    Bi l 2A03 L b 1 H N i i Wi T 2012

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

    This lab chapter was developed by and is the property of the Biology Department, McMaster University. Unauthorized copying of this laboratory chapter in its entirety or part is prohibited without permission of the

    Biology Department, McMaster University

    27 of 28

    Note: This is a semi-formal lab report, due at 5 pm ONE week from your regularly scheduled lab (January 23 27, ALL lab sections). Hand your lab report to your lab sections drop box

    located on the first floor of Life Sciences. It is your responsibility to ensure that your lab report is submitted to the correct drop box, that your report is complete and handed in on time.

    Late lab reports will not be marked and will receive a grade of zero. Reports submitted to the wrong drop box will be considered to be late and will NOT be marked.

    Biology 2A03 Lab Report 1 Marking (Partial Lab Report, worth 5%): Attach this marking rubric to your lab report

    Title Page Title of lab report, your name, id #, lab section, TAs name, date of lab & due date. Include this chart at the end of

    your report.

    Marking Category PageLength

    2

    TA Comments Mark

    Pre-lab Assignment: pages 12-16. 3 day Kilocalorie intake, one day kilocalorie expenditure charts and your personal BMR calculationcompleted and shown to your TA in Lab 1 tutorial. Your charts must have your TAs initials from the Lab 1 tutorial. If not initialed by TA at the time of

    Lab 1 tutorial, = 0/5/5

    Results How well is the data recorded? No calculations (put details of calculations in theAppendix, only the results of the calculations go in the Results). Description of results (but no

    interpretation). Data presentation - tables & graphs are included in the Results section.

    How well is the data recorded? Description of results as trends BUT NO INTERPRETATION,

    excessive data should be put in the Appendix. Graphs & Tables are not included in thepage limit. Limit the number of tables or graphs by combining data into fewer tables/graphs.

    Follow the J. of Exp. Biology guidelines for table or graph style. Tables & graphs must

    summarize the data.

    2 /40

    Conclusions (5%) What conclusions can you draw from this study? What

    recommendations can you make based on your data? - 1 /5

    2Any text exceeding these page limits will not be marked by your TA! Text must be Helvetica or Arial 12 pt, 1.5 spacing, margins 50 pt, top,

    bottom, left, right.

    Bi l 2A03 L b 1 H N t iti Wi t T 2012

  • 8/2/2019 Biology 2a03 - Lab 1 Outline 2012

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    Biology 2A03 Lab 1 Human Nutrition Winter Term 2012

    This lab chapter was developed by and is the property of the Biology Department, McMaster University. Unauthorized copying of this laboratory chapter in its entirety or part is prohibited without permission of the

    Biology Department, McMaster University

    28 of 28

    Note: This is a semi-formal lab report, due at 5 pm ONE week from your regularly scheduled lab (January 23 27, ALL lab sections). Hand your lab report to your lab sections drop box

    located on the first floor of Life Sciences. It is your responsibility to ensure that your lab report is submitted to the correct drop box, that your report is complete and handed in on time.

    Late lab reports will not be marked and will receive a grade of zero. Reports submitted to the wrong drop box will be considered to be late and will NOT be marked.

    Biology 2A03 Lab Report 1 Marking (Partial Lab Report, worth 5%): Attach this marking rubric to your lab report

    Title Page Title of lab report, your name, id #, lab section, TAs name, date of lab & due date. Include this chart at the end of

    your report.

    Marking Category PageLength

    2

    TA Comments Mark

    Appendix 5%raw data sheets, for lab 1 = 3 days of diet record (3) and one days worth

    of activity (1), any summary data & calculations (BMR calculations). The results of thecalculations go in the Results section. Label tables & Graphs in the Appendix with A1, A2

    etc. to differentiate them from tables & graphs in the Results section. (Pre-lab assignment

    pages go in the Appendix).

    5-6 /5

    Style, Layout, Overall Impression /Spelling 5%-grammar, spelling, and completesentences (not in point form)? - Neatness of layout, professional looking? - Logical statements? Use

    spell check Marking rubric included with lab report (marks lost if not included)

    /5

    PARTIAL LAB REPORT TOTAL = /60

    THEORY ASSIGNMENT TOTAL = /40

    LAB 1 TOTAL = /100

    1Any text exceeding these page limits will not be marked by your TA! Text must be Helvetica or Arial 12 pt, 1.5 spacingmargins 50 pt, top, bottom, left, right