biology lecture slide week 2
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Biology Lecture Slide Week 2TRANSCRIPT
CELL STRUCTURE AND FUNCTION OF ORGANELLES
IN THIS TOPIC
Structure and functions : Cell wall Cell membrane Organelles
Movement of molecules/substances in and out of the cell
CELL WALL
Outside the cell membrane
In plant cell, prokaryotes, some protista and fungi only.
Components : middle lamella primary wall secondary wall Plasmodesmata
Land plants : Cellulose Lignin Suberin
Waxy Waterproof (some tissue)
Functions : Cell shape Support and mechanical strength Prevent haemolysis ‘skin’ Carbohydrate store
CELL MEMBRANE
Both in animal and plant cell
Selective permeable
Composed of : Proteins Glycoprotein Glycolipids Phospholipid Cholesterol and lipoprotein (a few)
Fluid mosaic model
Lipid bilayer Phospholipid Hydrophilic head (polar) Hydrophobic tail (non-polar)
Embedded protein Embedded between layer Integral protein Peripheral protein (not embedded) Free movement within layer As carrier and channel protein
Cholesterol Make phospholipid stay in position
‘Fluid’ protein and lipid molecules able to shift and move laterally
‘Mosaic’ the protein molecules form mosaic-like patterns when viewed
from the top.
Functions of cell membrane
Barrier Maintain internal environment of
cell Communication between cell Markers to foreign materials Transportation
(Text book page 29)
ORGANELLES
1. Nucleus2. Endoplasmic reticulum3. Golgi apparatus4. Mitochondria5. Lysosomes6. Ribosomes7. Chloroplasts8. Centrioles9. Vesicles
Nucleus
Nucleus
‘The Control Centre’
Available in all eukaryotes excepts ; Sieve tube of phloem R_ _ _ _ oo _ _ _ l l
Components of nucleus
nuclear envelope
nucleoplasm
nucleolus
chromatin
Nuclear envelope
Two outer membranes (lipid bilayers) Innermost surface has DNA attachment
sites
Nuclear porebilayer facing cytoplasm Nuclear envelope
bilayer facing
nucleoplasm
Nucleolus dark spot in the middle of the nucleus produce ribosomes
Functions : Store genetics material and information
Site of cell division Produce RNA & DNA
Endoplasmic reticulum
In animal cells, continuous with
nuclear membrane
Extends throughout cytoplasm
Two regions - rough and smoothRough ER Smooth ER
Functions
ROUGH ER SMOOTH ER
Prominent in cells that manufacture protein for export ; digestive enzymes, hormones, structural protein or antibodies
Necessary for steroid synthesis, metabolism and detoxification of substances in liver, phospholipid synthesis and excitation-contraction coupling in skeletal muscle
With ribosome Without ribosome
Golgi body
Puts finishing touches on proteins and lipids that arrive from ER
Packages finished material for shipment to final destinations
Material arrives and leaves in vesicles
Mitochondria
ATP-producing powerhouses
(aerobic respiration)
Membranes form two distinct
compartments
ATP-making machinery
embedded in inner
mitochondrial membrane
outercompartment
innercompartment
outer membrane inner membrane
repeated foldings of inner membrane (cristae)
Lysosomes
a vesicle with enzymes digest biological macromolecules ER to Golgi body Autophagy Autolysis
Ribosomes
Function as the site of mRNA translation during protein synthesis
Free-wheeling in cytoplasm / attached to ER
TWO subunits ; small and large
Chloroplasts
Site of photosynthesis
Centrioles
Type of cytoskeleton (network of fiber) In mitosis; source of mitotic spindle Functions ;
Organise the formation of spindle fiber Organise the formation of cilia and flagella
(9+2 / 9+3)
vesicles
a sac In cytoplasm Types ;
Lysosome Peroxisome Shuttle vesicles Secretory vesicles Endocytosis vesicles Storage vesicles
♫ ♪ Take 5 ♫ ♪
MOVEMENT OF MOLECULES/SUBSTANCES IN AND OUT OF THE CELL
The movement of molecules are based on concentration gradient
Concentration gradient ; Means the number of molecules or ions in one
region is different than the number in another region
In the absence of other forces, a substance moves from a region where it is more concentrated to one where it’s less concentrated - “down” gradient
Passive transport
Active transport
Endocytosis
Exocytosis
PASSIVE TRANSPORT
Does not require ATP High to low of concentration gradient
Types ; Diffusion Osmosis Facilitated diffusion
Diffusion
movement of molecules or ions down a concentration gradient
the molecules move using a natural kinetic energy
what determines the rate of diffusion?
Steepness of concentration gradient◦ Steeper gradient, faster diffusion
Molecular size◦ Smaller molecules, faster diffusion
Temperature◦ Higher temperature, faster diffusion
Surface area◦ Higher surface area, faster diffusion
Molecules that diffuse through cell membranes
Oxygen – Non-polar so diffuses very quickly.
Carbon dioxide – Polar but very small so diffuses quickly.
Water – Polar but also very small so diffuses quickly.
Simple Diffusion
small, nonpolar molecules
(ex. O2, CO2)
Polar molecules(ex. Glucose, water)
ions(ex. H+, Na+, K+)
LIPID-SOLUBLE WATER-SOLUBLE
LIPID-SOLUBLE
Facilitated diffusion
movement of molecules or ions down a concentration gradient with the help of transport protein
Types of transport protein ; Carrier protein Channel protein
glucose transporter
solute (glucose)
low
[ conc’n] of glucose outside cell > inside
Glucose binds
Protein change shapeGlucose is exposed to fluid on the
other side of the membrane, detaches, leaves channel
Osmosis
Water movement across a partially permeable
membrane from an area of high
water potential (low solute concentration) to an area of low water potential (high solute concentration)
HypotonicSolution
membrane permeable towater but not to solutes
HypertonicSolution
Tonicity 2% sucrosesolution
distilled water
10%sucrose solution
2%sucrose solution
Hypotonic Conditions
Hypertonic Conditions
Isotonic Conditions
Isotonic solution Hypotonic solution Hypertonic solution
H2O H2O
(1) Normal (2) Lysed
H2O
H2O H2O H2O
Animalcell
Plantcell
(4) Flaccid (5) Turgid (6) Shriveled (plasmolyzed)
(3) Shriveled
Plasmamembrane
H2O
H2O
ACTIVE TRANSPORT
Transport of molecule
Low to high of concentration gradient
require ATP
Involves carrier protein
ATP
ADP
Pi
higher calcium concentration
lower calcium concentration
Calcium enter tunnel
Calcium pump
ATP transfer a phosphate group
Shape change –Ca release
Shape returns o its resting position
ENDOCYTOSIS
Transport of macromolecules
Involves formation of vesicle
Into the cell
Two types; Phagocytosis Pinocytosis
phagocyticvesicle
ediblebacterium
amoeba
Phagocytosis
Phagocytosis; “cellular eating”.
In phagocytosis, the cell engulfs a particle by extending pseudopodia around it and packaging it in a large vacuole.
The contents of the vacuole are digested when the vacuole fuses with a lysosome.
In pinocytosis; “cellular drinking”
a cell creates a vesicle around a droplet of extracellular fluid.
EXOCYTOSIS
Transport of molecules Out of a cell Enclosed in vesicle Move to cell surface membrane Fuse release
Both diffusion and facilitated diffusion are forms of passive transport of molecules down their concentration gradient, while active transport requires an investment of energy to move molecules against their concentration gradient.
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