biology practical part 2

2
Conclusions: Through visual inspection of the end colors of the known glucose concentrations and the unknown one, we came to the conclusion that the end color of the unknown glucose concentration was closer to the 0.125% glucose concentration color. Evaluation: The measurements of the volumes of the concentration is one of the significant sources of errors in this investigation, especially in the serial dilution method, if the volumes of the distilled water and the glucose concentration goes wrong, the final concentration of the glucose will not be accurate which will affect the whole investigation. Another source of error would be the temperature, since we did our investigation in two batches, three boiling tubes at a time, these boiling tubes could have placed into the water bath at slightly different temperature, which will not make this investigation a fair one. Another significant source of error would be the time each boiling tubes would react for after taking them out of the water bath, as we all know the reaction does not stop immediately once you take the boiling tubes out of the water bath, hence each batch of boiling tubes would take different time to cool down as they were placed into the water bath which was at slightly different temperatures. Besides the above, another source of error which will not change the results of this investigation too much would be how different people would interpret the colors. Improvements: Repeating the experiment a minimum of three times would make the results more reliable. A more accurate result would be produced when all the six boiling tubes were placed into the water bath at exactly the same time so that they will all be in the same temperature. Also ensuring that the temperature of the water bath remains the same throughout the five minutes of the experiment. In addition to that, the amount of time the boiling tubes continue to react for can also be monitored, after taking the boiling tubes out of the water bath, we can leave them for exactly a minute to cool down

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Conclusions:Through visual inspection of the end colors of the known glucose concentrations and the unknown one, we came to the conclusion that the end color of the unknown glucose concentration was closer to the 0.125% glucose concentration color.

Evaluation:The measurements of the volumes of the concentration is one of the significant sources of errors in this investigation, especially in the serial dilution method, if the volumes of the distilled water and the glucose concentration goes wrong, the final concentration of the glucose will not be accurate which will affect the whole investigation. Another source of error would be the temperature, since we did our investigation in two batches, three boiling tubes at a time, these boiling tubes could have placed into the water bath at slightly different temperature, which will not make this investigation a fair one. Another significant source of error would be the time each boiling tubes would react for after taking them out of the water bath, as we all know the reaction does not stop immediately once you take the boiling tubes out of the water bath, hence each batch of boiling tubes would take different time to cool down as they were placed into the water bath which was at slightly different temperatures. Besides the above, another source of error which will not change the results of this investigation too much would be how different people would interpret the colors. Improvements:Repeating the experiment a minimum of three times would make the results more reliable. A more accurate result would be produced when all the six boiling tubes were placed into the water bath at exactly the same time so that they will all be in the same temperature. Also ensuring that the temperature of the water bath remains the same throughout the five minutes of the experiment. In addition to that, the amount of time the boiling tubes continue to react for can also be monitored, after taking the boiling tubes out of the water bath, we can leave them for exactly a minute to cool down during which the mixture will continue to react and then place them in cold water, to cool the boiling tubes completely and the reaction is complete. All this should be done with the six boiling tubes at exactly the same time to ensure that the investigation remains a fair one. Another improvement would be using the colorimeter to determine which glucose concentrations’ color matches with the unknown glucose. After the reaction, we can pipette each of the six mixtures into six separate cuvettes and run the colorimeter, which will give us a quantities value which we can then use to compare which color is the closest to the unknown glucose concentration. This would give us a more accurate result as we are using quantative data to compare rather than visual inspection which can be subjective; the saturation of the colors can vary from person to person.