biosafety practices for the microbiology...
TRANSCRIPT
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Biosafety Practices for the Microbiology Laboratory
A Guide for Microbiology Students
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What are microbes?
Microorganisms such as bacteria, yeast and other fungi, viruses, and protozoans
Protozoan: Trypanosoma
Bacteria: Escherichia coli
Fungus: Saccharomyces
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Why do we need special handling for microbes?
Some microbes can cause infectious diseasesSpecial protocols must be implemented to minimize the risk to students, faculty, and staff
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Potential Routes of Transmission
Contact with skin and mucous membranes IngestionInhalationInoculation
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Precautions to Reduce Transmission
Wear personal protective equipment Strictly prohibit eating and drinking in the labMinimize aerosols and wear respirators when neededTake special precautions with sharps
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What types of microbes can be handled in our microbiology lab?
The Centers for Disease Control divides microbes into 4 Biosafety Levels based on their ability to cause diseaseOnly organisms designated BSL-1 and BSL-2 are used in Microbiology lab
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Biosafety Level 1
Organisms which are not known to cause disease in healthy humansEg: Staphylococcus epidermidis, Bacillus megaterium
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Biosafety Level 2
Moderate-risk organisms associated with less serious human diseases
Potential for transmission is limitedProven treatment for disease exists
Eg: Staphylococcus aureus, Streptococcus pyogenes
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Biosafety Level 3
High-risk organisms associated with serious or fatal human diseases
Potential for aerosol transmission is highVaccine or therapy is available
Eg: Mycobacterium tuberculosis, Bacillus anthracis
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Biosafety Level 4
Very high-risk organisms associated with life-threatening human diseases
Easily transmittedNo vaccine or therapy available for disease
Eg: Ebola and Marburg Hemorrhagic fever viruses
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What are the general safety guidelines when working with BSL-1 organisms?
Absolutely no food or drink in labStudents should practice sterile techniqueAll microbial waste should be discarded in orange BIOHAZARD bagsSharps must be disposed of in proper container
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Additional precautions for BSL-2 organisms
Personal Protective Equipment:Students must wear disposable glovesStudents must wear aprons or lab coats
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Additional precautions for BSL-2 organisms
Label ALL plates and tubes.Your name/date/class section.Name(s) of microorganisms.
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Additional precautions for BSL-2 organisms
Agar plates must be taped shut on both sides before placing in incubator.
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Additional precautions for BSL-2 organisms
Test tubes must always be transported in a test tube rack.
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All gloves must be disposed of in orange BIOHAZARD bag
Remove gloves inside-out.DO NOT THROW IN REGULAR TRASH!
Additional precautions for BSL-2 organisms
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Additional precautions for BSL-2 organisms
All contaminated sharps must be disposed of in red SHARPS container
Glass slidesBroken test tubes
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Additional precautions for BSL-2 organisms
All used test tubes must be placed in tube discard bin.
DO NOT pour cultures down the sink!Tubes will be autoclaved.
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Additional precautions for BSL-2 organisms
All used pipettes must be placed in pipette discard box.
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Accidents!Notify instructor immediately!Determine if anyone has been exposed and take appropriate measuresCover spills with paper towels and soak with bacteriocide.
Dispose of paper towels in Biohazard wasteDispose of broken glass in Sharps waste
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Questions?
Please Ask!