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  • 8/13/2019 Biosuccess Biotech v. Rich Pharmaceuticals et. al.

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    EXHIBIT A

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    Biosuccess Biotech Co. Ltd. | Biosuccess Biotech Co. Ltd.

    Home About Us Technology Studies Patients Medical Professionals News & Releases Investor Information Contact Us

    meout Usompany Historyadership Team

    hnologydies

    ML Stu diesDS Studiesents

    MLDefinitionPrognosisPreventionsCausesSymptomsDiagnosisTestsTreatmentsComplicationsDeathDS/HIVDefinitionPrognosisPreventionsCausesSymptomsDiagnosisTestsTreatmentsComplicationsDeathood Cancerne Cancer

    dical ProfessionalsML DocumentsDS Documentsntact UsQs

    About Us

    Founded in 2005, Biosuccess Biotech Co. Ltd. is a privately held company. It focuses on the

    development of PD-616 (12-O-tetradecanoylphorbol-13-acetate, also known as TPA) for the

    treatment ofAMLandAIDS/HIV .The work of two scientists, Prof. Richard Chang & Prof.

    Zhang Tao Han sustained by their knowledge of this agents characteristics and their wide

    experience of years is the basis for the companys interest in using PD-616 in treatment.

    AML Studies wi th TPA (PD-616)

    Clinical studies were conducted in China in patients who had few (sometimes none)

    remaining options for therapy in acute myelogenous leukemia (AML). These patients were

    refractory to standard therapy and had debilitating symptoms. Findings showed that some

    patients were put into partial remission and established the short term safety for the

    intravenous admini stration of PD-616.

    Encouraged by the clinical results from China, Roger Strair MD, PhD, a leading oncologist at

    The Cancer Institute of New Jersey (CINJ), at the University of Medicine and Dentistry of

    N.J. (UMDNJ), obtained an investigator IND, and conducted a Phase 1 study in patients the

    majority of whom also had relapsed/refractory AML. Based on Phase 1 findings, BiosuccessBiotech was encouraged by Dr. Strair to conduct a Phase 2 study in relapsed/refractory AML.

    Currently, a Phase 2 study is underway and the recruitment of refractory AML patients is

    actively done by Dr. Strair.

    AIDS/HIV Studies wi th TPA (PD-616)

    Three preliminary experiments, part of the clinical research process, were conducted in China

    on AIDS/HIV. These patients had few treatment options, as they presented many of the

    injurious effects of this disease and were refractory to standard anti-AIDS drugs. Following

    treatment wit h PD-616, there was a disappearance of symptoms and a return to normal

    in almost all patients. In t he third cl inical stu dy, the number of CD4 T-cells was

    somewhat reduced while the concentration of the virus in blood increased in all

    patients in 30 days after st arting PD-616 treatment, but was almost undetectable at 60

    days. These results are clinical evidence that support the unique mechanism of action

    proposed for PD-616 in AIDS/HIV. Future clinical studies are needed, but it appears that PD-

    616 can be a totally new and highly effective drug for the treatment of AIDS. In order to

    confirm these clinical findings, Biosuccess Biotech Co. Ltd. has plans to conduct rigorously

    controlled clinical trials in the U.S. during a large Phase 2 study.

    Legal Grounds

    Biosuccess Biotech is protected by an issued use patent that provides sole rights to use the

    intravenous administration of PD-616 for therapeutic purposes (2001).

    A patent application was filed on the use of PD-616 to treat AML in January, 2007. In

    January, 2008, a patent application was filed for the use of PD-616 in HIV/AIDS and includes

    coverage of many other phorbol ester structures in the PD-616 chemical class.

    Biosuccess Biotech Co. Ltd. has 20 years exclusive right s to t he use of PD-616 in AML

    and HIV/AIDS, beginning with January 30th, 2008.

    In June 2011, AML Phase 2 protocol was approved by FDA.

    Company History

    Leadership Team

    Latest News

    April 27th, 2012

    Biosuccess has received an IND approval fro

    FDA. An Acute Myelocytic Leukemia (AML) studythis IND will be underway shortly.

    March 23rd, 2012

    A corporate IND has been submitted to the FDA f

    of TPA in Acute Myelocytic Leukemia (AML).

    June 16th, 2011

    Jace Chew joins our team as President of A

    India sub-continent and Australia operations.

    June 16th, 2011

    AML Phase 2 trial begins recruiting patients.

    Read More News

    opyright 2011 BioSuccessBioTech.com. All rights reserved. Sitemap

    http://www.biosuccessbiotech.com/http://users/kaseekinzler/Desktop/About%20Biosuccess%20Biotech%20Co.%20Ltd.%20%20%20Biosuccess%20Biotech%20Co.%20Ltd._files/About%20Biosuccess%20Biotech%20Co.%20Ltd.%20%20%20Biosuccess%20Biotech%20Co.%20Ltd..htmlhttp://www.biosuccessbiotech.com/technology/http://www.biosuccessbiotech.com/studies/http://www.biosuccessbiotech.com/patients/http://www.biosuccessbiotech.com/medical-professionals/http://www.biosuccessbiotech.com/news-releases/http://www.biosuccessbiotech.com/investor-information/http://www.biosuccessbiotech.com/contact-us/http://users/kaseekinzler/Desktop/About%20Biosuccess%20Biotech%20Co.%20Ltd.%20%20%20Biosuccess%20Biotech%20Co.%20Ltd._files/About%20Biosuccess%20Biotech%20Co.%20Ltd.%20%20%20Biosuccess%20Biotech%20Co.%20Ltd..htmlhttp://www.biosuccessbiotech.com/about-us/company-history/http://www.biosuccessbiotech.com/about-us/leadership-team/http://www.biosuccessbiotech.com/about-us/leadership-team/http://www.biosuccessbiotech.com/studies/http://www.biosuccessbiotech.com/technology/http://www.biosuccessbiotech.com/studies/aml/http://www.biosuccessbiotech.com/studies/aids/http://www.biosuccessbiotech.com/patients/http://www.biosuccessbiotech.com/studies/aids/http://www.biosuccessbiotech.com/patients/http://www.biosuccessbiotech.com/aml/definition/http://www.biosuccessbiotech.com/aml/prognosis/http://www.biosuccessbiotech.com/aml/preventions/http://www.biosuccessbiotech.com/aml/causes/http://www.biosuccessbiotech.com/aml/preventions/http://www.biosuccessbiotech.com/aml/causes/http://www.biosuccessbiotech.com/aml/diagnosis/http://www.biosuccessbiotech.com/aml/tests/http://www.biosuccessbiotech.com/aml/diagnosis/http://www.biosuccessbiotech.com/aml/treatments/http://www.biosuccessbiotech.com/aml/complications/http://www.biosuccessbiotech.com/aml/complications/http://www.biosuccessbiotech.com/aids/http://www.biosuccessbiotech.com/aids/definition/http://www.biosuccessbiotech.com/aids/prognosis/http://www.biosuccessbiotech.com/aids/preventions/http://www.biosuccessbiotech.com/aids/preventions/http://www.biosuccessbiotech.com/aids/symptoms/http://www.biosuccessbiotech.com/aids/diagnosis/http://www.biosuccessbiotech.com/aids/tests/http://www.biosuccessbiotech.com/aids/diagnosis/http://www.biosuccessbiotech.com/aids/treatments/http://www.biosuccessbiotech.com/aids/treatments/http://www.biosuccessbiotech.com/aids/death/http://www.biosuccessbiotech.com/aids/complications/http://www.biosuccessbiotech.com/blood-cancer/http://www.biosuccessbiotech.com/bone-cancer/http://www.biosuccessbiotech.com/medical-professionals/http://www.biosuccessbiotech.com/medical-professionals/http://www.biosuccessbiotech.com/medical-professionals/aids/http://www.biosuccessbiotech.com/contact-us/http://www.biosuccessbiotech.com/medical-professionals/aids/http://www.biosuccessbiotech.com/faqs/http://www.biosuccessbiotech.com/contact-us/http://www.biosuccessbiotech.com/faqs/http://www.biosuccessbiotech.com/aml/http://www.biosuccessbiotech.com/aids/http://www.biosuccessbiotech.com/aids/http://www.biosuccessbiotech.com/studies/aml/http://www.biosuccessbiotech.com/studies/aml/http://www.biosuccessbiotech.com/about-us/company-history/http://www.biosuccessbiotech.com/about-us/leadership-team/http://www.biosuccessbiotech.com/news-releases/http://www.biosuccessbiotech.com/sitemap-2/http://www.biosuccessbiotech.com/http://www.biosuccessbiotech.com/sitemap-2/http://www.biosuccessbiotech.com/news-releases/http://www.biosuccessbiotech.com/studies/aids/http://www.biosuccessbiotech.com/studies/aml/http://www.biosuccessbiotech.com/patients/http://www.biosuccessbiotech.com/medical-professionals/http://www.biosuccessbiotech.com/news-releases/http://www.biosuccessbiotech.com/about-us/leadership-team/http://www.biosuccessbiotech.com/about-us/company-history/http://www.biosuccessbiotech.com/studies/aml/http://www.biosuccessbiotech.com/aids/http://www.biosuccessbiotech.com/aml/http://www.biosuccessbiotech.com/faqs/http://www.biosuccessbiotech.com/contact-us/http://www.biosuccessbiotech.com/medical-professionals/aids/http://www.biosuccessbiotech.com/medical-professionals/aml/http://www.biosuccessbiotech.com/medical-professionals/http://www.biosuccessbiotech.com/bone-cancer/http://www.biosuccessbiotech.com/blood-cancer/http://www.biosuccessbiotech.com/aids/death/http://www.biosuccessbiotech.com/aids/complications/http://www.biosuccessbiotech.com/aids/treatments/http://www.biosuccessbiotech.com/aids/tests/http://www.biosuccessbiotech.com/aids/diagnosis/http://www.biosuccessbiotech.com/aids/symptoms/http://www.biosuccessbiotech.com/aids/causes/http://www.biosuccessbiotech.com/aids/preventions/http://www.biosuccessbiotech.com/aids/prognosis/http://www.biosuccessbiotech.com/aids/definition/http://www.biosuccessbiotech.com/aids/http://www.biosuccessbiotech.com/aml/death/http://www.biosuccessbiotech.com/aml/complications/http://www.biosuccessbiotech.com/aml/treatments/http://www.biosuccessbiotech.com/aml/tests/http://www.biosuccessbiotech.com/aml/diagnosis/http://www.biosuccessbiotech.com/aml/symptoms/http://www.biosuccessbiotech.com/aml/causes/http://www.biosuccessbiotech.com/aml/preventions/http://www.biosuccessbiotech.com/aml/prognosis/http://www.biosuccessbiotech.com/aml/definition/http://www.biosuccessbiotech.com/aml/http://www.biosuccessbiotech.com/patients/http://www.biosuccessbiotech.com/studies/aids/http://www.biosuccessbiotech.com/studies/aml/http://www.biosuccessbiotech.com/studies/http://www.biosuccessbiotech.com/technology/http://www.biosuccessbiotech.com/about-us/leadership-team/http://www.biosuccessbiotech.com/about-us/company-history/http://users/kaseekinzler/Desktop/About%20Biosuccess%20Biotech%20Co.%20Ltd.%20%20%20Biosuccess%20Biotech%20Co.%20Ltd._files/About%20Biosuccess%20Biotech%20Co.%20Ltd.%20%20%20Biosuccess%20Biotech%20Co.%20Ltd..htmlhttp://www.biosuccessbiotech.com/http://www.biosuccessbiotech.com/contact-us/http://www.biosuccessbiotech.com/investor-information/http://www.biosuccessbiotech.com/news-releases/http://www.biosuccessbiotech.com/medical-professionals/http://www.biosuccessbiotech.com/patients/http://www.biosuccessbiotech.com/studies/http://www.biosuccessbiotech.com/technology/http://users/kaseekinzler/Desktop/About%20Biosuccess%20Biotech%20Co.%20Ltd.%20%20%20Biosuccess%20Biotech%20Co.%20Ltd._files/About%20Biosuccess%20Biotech%20Co.%20Ltd.%20%20%20Biosuccess%20Biotech%20Co.%20Ltd..htmlhttp://www.biosuccessbiotech.com/
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    About Us | Rich Pharmaceuticals.com

    www.richpharmaceuticals.com/about-us/ 1

    About Us Technology Studies Patients Medical Professionals News & Releases Investor Information Contact Us

    Headline is editable in the InnerBanner Tab in the Dashboard

    This text is also editable in the same page

    The background image is also editable.

    Rich Pharmaceuticals.com About Us

    About UsRich Pharmaceuticals is a biopharmaceutical company that became a public entity August 26, 2012 as a

    result of a reverse merger with Nepia Inc. The Company is focused on the development of its lead product,

    TPA (12-O-tetradecanoylphorbol-13-acetate), for the treatment of acute myelogenous leukemia (AML) in

    refractory patients, and the reversal of physical disabilities resulting from stroke. The basis for the interest

    of the Company to pursue clinical development of TPA in these and possibly other indications is the result

    of the work of two scientists, Prof. Richard Chang and Prof. Zhang Tao Han. Both have conducted research

    on TPA for many years and have become experts in the characteristics of this molecule. Their findings form

    the scientific basis for the clinical use of TPA.

    AML Studies with TPA

    Initially, clinical studies were conducted in leading hospitals in China in patients who had few, if any, options

    remaining for the treatment of AML. These patients were refractory to standard therapy and had debilitating

    symptoms that were life threatening. The clinical status of some of the patients treated with TPA changed

    favorably to partial remission. In addition, the short term safety of TPA was established in these individuals

    using an intravenous formulation of TPA.

    Encouraged by the clinical results with TPA in China, Roger Strair MD, PhD, a leading oncologist at the

    University of Medicine and Dentistry at Rutgers University, obtained an investigator IND and successfully

    completed a Phase 1 study in patients, the majority of whom had relapsed/refractory AML. Rich

    Pharmaceuticals was encouraged by Dr. Strair to conduct a Phase 2 study in relapsed/refractory AML. A

    Phase 2 study is currently underway under the direction of Dr. Strair who is actively enrolling appropriate

    patients in this study.

    Legal GroundsRich Pharmaceuticals is protected by an issued use patent that gives sole rights to the Company to use the

    intravenous administration of TPA for therapeutic purposes. Since TPA can only be administered for

    therapeutic purposes by this route, this patent provides complete protection for the use of TPA for any

    other use.

    About UsCompany H istory

    Leadership Team

    NewsOctober 2nd, 2013

    Rich Pharmaceuticals Inc. has

    been licensed to pursue the

    clinical development of their

    lead drug, TPA, in acute

    myelogenous leukemia (AML)

    and stroke.

    October 1st, 2013

    Rich Pharmaceuticals appoints

    Robert Thomas as COO

    September 6th, 2013

    Rich Pharmaceuticals appoints

    David Chou, Phd. to the Board

    of Director

    View All News

    Morbi accumsan convallis est,

    sit amet varius sapien

    commodo.

    Read More

    Stock Ticker

    http://www.richpharmaceuticals.com/about-us/http://www.richpharmaceuticals.com/technology/http://www.richpharmaceuticals.com/studies/http://www.richpharmaceuticals.com/patients/http://www.richpharmaceuticals.com/medical-professionals/http://www.richpharmaceuticals.com/category/news/http://www.richpharmaceuticals.com/investor-information/http://www.richpharmaceuticals.com/contact-us/http://www.richpharmaceuticals.com/http://www.richpharmaceuticals.com/about-us/company-history/http://www.richpharmaceuticals.com/about-us/leadership-team/http://www.richpharmaceuticals.com/category/news/http://www.richpharmaceuticals.com/category/news/http://www.richpharmaceuticals.com/about-us/leadership-team/http://www.richpharmaceuticals.com/about-us/company-history/http://www.richpharmaceuticals.com/http://www.richpharmaceuticals.com/contact-us/http://www.richpharmaceuticals.com/investor-information/http://www.richpharmaceuticals.com/category/news/http://www.richpharmaceuticals.com/medical-professionals/http://www.richpharmaceuticals.com/patients/http://www.richpharmaceuticals.com/studies/http://www.richpharmaceuticals.com/technology/http://www.richpharmaceuticals.com/about-us/http://www.richpharmaceuticals.com/
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    About Us | Rich Pharmaceuticals.com

    www.richpharmaceuticals.com/about-us/ 2

    Contact Us

    Contact Rich Pharmaceuticals by going to our contact us page.

    Sitemap

    About Us

    Technology

    Studies

    PatientsMedical Professionals

    News

    Investor Information

    Contact Us

    Resources

    Leadership Team

    Frequently Asked

    Questions

    Rich Pharmaceuticals. All Rights Reserved.

    Enter Symbol

    Q uo te We b

    RCHAD- OBB $N/A

    INTRADAY CHART

    http://www.richpharmaceuticals.com/contact-us/http://www.richpharmaceuticals.com/about-us/http://www.richpharmaceuticals.com/technology/http://www.richpharmaceuticals.com/studies/http://www.richpharmaceuticals.com/patients/http://www.richpharmaceuticals.com/medical-professionals/http://www.richpharmaceuticals.com/category/news/http://www.richpharmaceuticals.com/investor-information/http://www.richpharmaceuticals.com/contact-us/http://www.richpharmaceuticals.com/about-us/leadership-team/http://www.richpharmaceuticals.com/faqs/http://www.richpharmaceuticals.com/faqs/http://finance.yahoo.com/exchangeshttp://finance.yahoo.com/badgeshttp://us.rd.yahoo.com/finance/badge/chart/*http://finance.yahoo.com/q?s=RCHADhttp://us.rd.yahoo.com/finance/badge/financehome/*http://finance.yahoo.comhttp://www.richpharmaceuticals.com/faqs/http://www.richpharmaceuticals.com/about-us/leadership-team/http://www.richpharmaceuticals.com/contact-us/http://www.richpharmaceuticals.com/investor-information/http://www.richpharmaceuticals.com/category/news/http://www.richpharmaceuticals.com/medical-professionals/http://www.richpharmaceuticals.com/patients/http://www.richpharmaceuticals.com/studies/http://www.richpharmaceuticals.com/technology/http://www.richpharmaceuticals.com/about-us/http://www.richpharmaceuticals.com/contact-us/
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    EXHIBIT B

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    Alamilla : About Us

    Home About Us Technology Studies Patients Medical Professionals News & Releases Investor Information Contact Usmeout Usompany Historyadership Teamhnology

    diesML StudiesDS Studiesents

    MLDefinitionPrognosisPreventionsCausesSymptomsDiagnosisTestsTreatmentsComplicationsDeathDS/HIVDefinitionPrognosisPreventionsCausesSymptomsDiagnosisTestsTreatmentsComplicationsDeathood Cancerone Cancerdical ProfessionalsML DocumentsDS Documentsntact UsQs

    About Us

    Founded in 2005, Biosuccess Biotech Co. Ltd. is a privately held company. It focuses on the

    development of PD-616 (12-O-tetradecanoylphorbol-13-acetate, also known as TPA) for the

    treatment of AMLand AIDS/HIV.The work of two scientists, Prof. Richard Chang & Prof.

    Zhang Tao Han sustained by their knowledge of this agents characteristics and their wide

    experience of years is the basis for the companys interest in using PD-616 in treatment.

    AML Studies with TPA (PD-616)

    Clinical studies were conducted in China in patients who had few (sometimes none)

    remaining options for therapy in acute myelogenous leukemia (AML). These patients were

    refractory to standard therapy and had debilitating symptoms. Findings showed that some

    patients were put into partial remission and established the short term safety for the

    intravenous administration of PD-616.

    Encouraged by the clinical results from China, Roger Strair MD, PhD, a leading oncologist at

    The Cancer Institute of New Jersey (CINJ), at the University of Medicine and Dentistry of

    N.J. (UMDNJ), obtained an investigator IND, and conducted a Phase 1 studyin patients the

    majority of whom also had relapsed/refractory AML. Based on Phase 1 findings, BiosuccessBiotech was encouraged by Dr. Strair to conduct a Phase 2 study in relapsed/refractory AML.

    Currently, a Phase 2 study is underway and the recruitment of refractory AML patients is

    actively done by Dr. Strair.

    AIDS/HIV Studies with TPA (PD-616)

    Three preliminary experiments, part of the clinical research process, were conducted in China

    on AIDS/HIV. These patients had few treatment options, as they presented many of the

    injurious effects of this disease and were refractory to standard anti-AIDS drugs. Following

    treatment with PD-616, there was a disappearance of symptoms and a return to normal

    in almost all patients. In the third clinical study, the number of CD4 T-cells was

    somewhat reduced while the concentration of the virus in blood increased in all

    patients in 30 days after starting PD-616 treatment, but was almost undetectable at 60

    days. These results are clinical evidence that support the unique mechanism of action

    proposed for PD-616 in AIDS/HIV. Future clinical studies are needed, but it appears that PD-

    616 can be a totally new and highly effective drug for the treatment of AIDS. In order to

    confirm these clinical findings, Biosuccess Biotech Co. Ltd. has plans to conduct rigorously

    controlled clinical trials in the U.S. during a large Phase 2 study.

    Legal Grounds

    Biosuccess Biotech is protected by an issued use patent that provides sole rights to use the

    intravenous administration of PD-616 for therapeutic purposes (2001).

    A patent application was filed on the use of PD-616 to treat AML in January, 2007. In

    January, 2008, a patent application was filed for the use of PD-616 in HIV/AIDS and includes

    coverage of many other phorbol ester structures in the PD-616 chemical class.

    Biosuccess Biotech Co. Ltd. has 20 years exclusive rights to the use of PD-616 in AML

    and HIV/AIDS, beginning with January 30th, 2008.

    In June 2011, AML Phase 2 protocol was approved by FDA.

    Company History

    Leadership Team

    Latest News

    Read More News

    opyright 2011 Rich Pharmaceuticals.com. All rights reserved. Sitemap

    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aceuticals.com/patients/http://www.richpharmaceuticals.com/studies/aids/http://www.richpharmaceuticals.com/studies/aml/http://www.richpharmaceuticals.com/studies/http://www.richpharmaceuticals.com/technology/http://www.richpharmaceuticals.com/about-us/leadership-team/http://www.richpharmaceuticals.com/about-us/company-history/http://www.richpharmaceuticals.com/about-us/http://www.richpharmaceuticals.com/http://benalamilla.me/contact-us/http://benalamilla.me/investor-information/http://benalamilla.me/news-releases/http://benalamilla.me/medical-professionals/http://benalamilla.me/patients/http://benalamilla.me/studies/http://benalamilla.me/technology/http://users/kaseekinzler/Desktop/Ben%20Alamilla%20%20%20About%20Us_files/Ben%20Alamilla%20%20%20About%20Us.htmlhttp://benalamilla.me/
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    EXHIBIT C

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    US006063814AUnited States Patent [ 1 9 ] [ 1 1 ] P a t e n t N u m b e r : 6 , 0 6 3 , 8 1 4Chang e t a l . [ 4 5 ] D a t e o f P a t e n t : May 6 , 2 0 0 0[ 5 4 ] PHORBOL ESTERS A S ANTI-NEOPLASTIC [ 5 6 ] R e f e r e n c e s C i t e d2 ? g i S I I T E B L O O D C E L L E L E V A T I N G P U B L I C A T I O N S

    _ S h i h e t a l . , C a r c i n o g e n e s i s ( 1 9 9 3 ) , 1 4 ( 4 ) , 7 0 9 1 2 , 1 9 9 3 .[ 7 6 ] I n v e n t o r s : R i c h a r d L . Chang, 1 0 7 K o n n e r A v e . ,P i n e B r o o k , N J . 0 7 0 5 8 ; Zheng T 3 0 P r i m a r y E x a m i n e r J e r o m e D . G o l d b e r gH a n , 4 Dongming R o a d , Z h e n g Z h o u , A t t o r n e y , A g e n t , o r F i r m B e r n a r d S . L e o nH e n a n , C h i n a [ 5 7 ] ABSTRACT

    [ 2 1 ] A p p l - N93 0 8 / 8 3 7 , 0 8 5 P h o r b o l e s t e r s a n d p a r t i c u l a r l y p h o r b o l - 1 2 - m y r i s t a t e - 1 3[ 2 2 ] F i l e d A p r - 1 4 1 9 9 7 a c e t a t e ( T P A ) a r e d e s c r i b e d a s e f f e c t i v e i n t r e a t i n g p a t i e n t s

    With neopla stic diseases such a s leukemia as Well a s i n[ 5 1 ] I n t . C l . 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A61K 1/21 i n c r e a s i n g t h e White blood c e l l c o u n t .[52] . . . . . . 514/510[ 5 8 ] F i e l d of Search . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 514/510 2 0 Claims, N0 Drawings

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    6 , 0 6 3 , 8 1 41

    PHORBOL ESTERS AS ANTI-NEOPLASTICAND WHITE BLOOD CELL ELEVATING

    AGENTSBRIEF DESCRIPTION OF THE INVENTION

    The i n v e n t i o n r e l a t e s t o t r e a t i n g n e o p l a s t i c d i s e a s e s u c h a sl e u k e m i a a n d i n c r e a s i n g t h e W h i t e b l o o d c e l l c o u n t s i np a t i e n t s s u f f e r i n g f r o m n e o p l a s t i c d i s e a s e s o r u n d e r g o i n gc h e m o t h e r a p y b y a m e t h o d W h i c h c o m p r i s e s a d m i n i s t e r i n gp a r e n t e r a l l y t o p a t i e n t s a n e f f e c t i v e amount o f a p h o r b o le s t e r o f t h e F o r m u l a :

    10

    1 5

    25

    or isomers thereof 3 0

    W h e r e i n R 1 a n d R2 a r e s e l e c t e d f r o m t h e g r o u p c o n s i s t i n go f h y d r o g e n ,

    0 35

    OCalkyl

    W h e r e i n t h e a l k y l g r o u p c o n t a i n s 1 t o 1 5 c a r b o n a t o m s , 4 0

    OCloWer l k e n y l , OCphenyl,45

    OCbenzyl

    and s u b s t i t u t e d d e r i v a t i v e s t h e r e o f . At l e a s t one o f R1 5 0a n d R2 s o t h e r t h a n h y d r o g e n a n d R 3 i s s e l e c t e d f r o mt h e g r o u p c o n s i s t i n g o f h y d r o g e n a n d

    55C-l oWer l k y l .

    P r e f e r r e d a r e Comp ounds o f t h e Formula Wherein one o fR 1 a n d R 2 i s s e l e c t e d f r o m t h e g r o u p c o n s i s t i n g o f

    60

    a n d R 3 i s h y d r o g e n .E s p e c i a l l y p r e f e r re d i s a compound f t h e f o r m u l a I W h e r e

    R 1 : OC(CH2)12CH3 i . e . m y r i s t a t e )

    i . e . a c e t a t e )

    i . e . p h o r b o l - 1 2 - m y r i s t a t e - 1 3 - a c e t a t e o r a s i t i s a l s o knoWn1 2 - O - t e t r a d e c a n o y l p h o r b o l - 1 3 - a c e t a t e h e r e i n T P A )The t e r m l o W e r a l k y l o r l o W e r a l k e n y l a s used h e r e i ns h a l l mean m o i e t i e s c o n t a i n i n g 17 c a r b o n a t o m s . I n t h eCompounds o f t h e F o r m u l a I , t h e a l k y l o r a l k e n y l g r o u p smay b e s t r a i g h t o r b r a n c h e d c h a i n a n d p r e f e r a b l y c o n t a i n a tl e a s t o n e o f R 1 o r R 2 , a l o n g c h a i n c a r b o n m o i e t y ( d e c a n o a t e

    o r m y r i s t a t e ) .T h e a l k y l , a l k e n y l , p h e n y l a n d b e n Z y l g r o u p s may b e

    u n s u b s t i t u t e d o r s u b s t i t u t e d W i t h h a l o g e n , p r e f e r a b l y ,c h l o r i n e , ? u o r i n e o r b r o m i n e , n i t r o , a m i n o a n d s i m i l a r t y p er a d i c a l s .

    BACKGROUND OF THE INVENTIONThe compounds o f t h e Formula a r e g e n e r a l l y knoWn t o

    b e t u m o r p r o m o t e r s a n d a s b e i n g h i g h l y i r r i t a n t t o s k i n a n dt h e m u c o u s membrane.The p r e f e r r e d e x e m p l a r TPA i s a b i o l o g i c a l l y a c t i v en a t u r a l compound Which c a n be e x t r a c t e d from c r o t o n o i l .TPA a s been knoWn f o r many y e a r s t o be a c o - c a r c i n o g e no r t u m o r p r o m o t e r . S e e Merck I n d e x , 1 1 t h E d i t i o n , P a g e1164 No. 7 3 0 6 . I t i s a l s o knoWn o b e a h i g h l y p o t e n t i r r i t a n tt o s k i n a n d t o b e h a r m f u l i f i n g e s t e d o r a l l y . I n a p r o d u c tb r o c h u r e d i s t r i b u t e d b y Chemsyn S c i e n c e L a b o r a t o r i e s o fL e n e x a , K a n s a s , TPA s d e s c r i b e d a s a n e x t r e m e l y p o t e n tmouse s k i n c a n c e r prom oter and a s a poWerful m itogen i nc e l l c u l t u r e s . The product brochure Warns t h e u s e r t o t r e a tTPA With extreme c a r e . The l i t e r a t u r e d i s c l o s e s t h a t TPAi n d u c e s d i f f e r e n t i a t i o n i n t h e s t a b l e human p r o m y e l o c y t i cl e u k e m i c c e l l l i n e HL-60. W e i n b e r g , J P ( S c i e n c e2 1 3 : 6 5 5 6 5 7 , 1 9 8 1 ) f u r t h e r d i s c l o s e s t h a t TPA c a u s e s d i ff e r e n t i a t i o n o f c e l l s o f t h e human l e u k e m i a c e l l l i n e HL-60t o n o n d i v i d i n g m a c r o p h a g e - l i k e c e l l s . T h e s e d i f f e r e n t i a t e dc e l l s a r e c y t o t o x i c f o r tumor c e l l s i n c l u d i n g c u r r e n t ,u n t r e a t e d HL-60 c e l l s i n v i t r o . HoWever, noWhere i n t h ep r i o r a r t h a s i t b e e n s u g g e s t e d t h a t compounds o f t h eFormula When d e l i v e r e d p a r e n t e r a l l y t o humans Would b ee f f e c t i v e i n t r e a t i n g n e o p l a s t i c d i se a s e s o r i n r a i s i n g t h eW h i t e b l o o d c e l l c o u n t , much l e s s W i t h o u t s i g n i ? c a n tunWanted s i d e e f f e c t s .

    Leukemia s a n e o p l a s t i c d i s e a s e i n Which W h i t e c o r p u s c l em a t u r a t i o n i s a r r e s t e d a t a p r i m it i v e s t a g e o f c e l l d e v e l o pm e n t . The d i s e a s e i s c h a r a c t e r i Z e d by a n i n c r e a s e d numbero f l e u k e m i c b l a s t c e l l s i n t h e b o n e marroW a n d by v a r y i n gd e g r e e s o f f a i l u r e t o p r o d u c e n o r m a l h e m a t o p o i e t i c c e l l s .The c o n d i t i o n may e e i t h e r a c u t e o r c h r o n i c . Leukemias a r ef u r t h e r t y p i c a l l y c h a r a c t e r i Z e d a s b e i n g l y m p h o c y t i c o r

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    6 , 0 6 3 , 8 1 43

    from bone m a r r ow h e m a t o p o i e t i c stem c e l l s o r t h e i r proge n y . The t e r m a c u t e m y e l o c y t i c leukemia subsumes s e ve r a l s u b t y p e s o f l e u k e m i a e . g . m y e l o b l a s t i c l e u k e m i a , p r om y e l o c y t i c l e u k e m i a a n d m y e l o m o n o c y t i c l e u k e m i a .

    C h r o n i c m y e l o g e n o u s l e u k e m i a i s c h a r a c t e r i z e d b y a b n o rm a l p r o l i f e r a t i o n o f i m m a t u r e g r a n u l o c y t e s , f o r e x a m p l e ,n e u t r o p h i l s , e o s i n o p h i l s a n d b a s o p h i l s , i n t h e b l o o d , b o n em a r r o W , t h e s p l e e n , l i v e r a n d sometimes i n o t h e r t i s s u e s . Al a r g e p o r t i o n o f c h r o n i c m y e l o g e n o u s l e u k e m i a p a t i e n t sd e v e l o p a t r a n s f o r m a t i o n i n t o a p a t t e r n i n d i s t i n g u i s h a b l efrom t h e a c u t e form o f t h e d i s e a s e . T h i s change s knoWn a st h e b l a s t c r i s e s : The p r e s e n t i n v e n t i o n i s g e n e r a l l y s u i t a b l ef o r t r e a t i n g l e u k e m i a s , a s W e l l a s o t h e r n e o p l a s t i c d i s e a s e s .

    DETAILED DESCRIPTION OF THEINVENTION

    Comp ounds o f t h e Formula I a r e u s e f u l a s a n t i - t u m o ra g e n t s i n p a t i e n t s s u f f e r i n g f r o m n e o p l a s t i c d i s e a s e s a n d f o rr a i s i n g t h e W h i t e b l o o d c e l l c o u n t i n p a t i e n t s s u f f e r i n g f r o mn e o p l a s t i c d i s e a s e s s u c h a s l e u k e m i a and o t h e r f o r m s o ft u m o r s s u c h a s s o l i d t u m o r s a n d u n d e r g o i n g c h e m o t h e r a p y .

    T h e p r e f e r r e d compound TPA h a s d e m o n s t r a t e d i nhumans t h e a b i l i t y t o r e d u c e t h e abnormal bone marroWp r o ? l e i n p a t i e n t s W i t h AML n d o t h e r t y p e s o f l e u k e m i a t ot h e p o i n t Where t h e p a t i e n t c a n b e c o n s i d e r e d t o b e i nr e m i s s i o n . Of t h e p a t i e n t s t r e a t e d W i t h TPA, a l l h a d b e e nd i a g n o s e d a s h a v i n g p r o g r e s s e d t o a n a c u t e form o f l e u k emia and t h e p r o g n o s i s f o r a f a v o r a b l e outcome Was n o t veryb r i g h t . P r i o r t o t h e a d m i n i s t r a t i o n o f TPA, l l o f t h e p a t i e n t sh a d r e c e i v e d v a r i o u s f o r m s o f c o n v e n t i o n a l c h e m o t h e r a p yi n c l u d i n g h y d r o x y u r e a , b u s u l f a n a n d Ara-C e t c W i t h o u ts u c c e s s o r i g i n a l l y o r b e c a u s e o f t h e development o f r e s i st a n c e t o t h e s e d r u g s . Upon a d m i n i s t r a t i o n o f TPA o t h e s er e f r a c t o r y p a t i e n t s , c l i n i c a l r e m i s s i o n Was a c h i e v e d i n ar e l a t i v e l y s h o r t t i m e . I n a d d i t i o n , d u r i n g a n d a f t e r t h et r e a t m e n t With TPA, t h e r e Was no bone m a r r oW s u p p r e s s i o n ,i n f e c t i o n o r b l e e d i n g . Many o f t h e p a t i e n t s h a v e b e e n i nc l i n i c a l r e m i s s i o n f o r o v e r s i x months from t h e t i m e t h et r e a t m e n t W i t h TPA r s t s t a r t e d .

    A d d i t i o n a l l y , t h e Compounds o f t h e Formula I c a n b eu s e d t o t r e a t p a t i e n t s Who a r e u n d e r g o i n g c h e m o t h e r a p y f o rt h e t r e a t m e n t o f s o l i d tumors a s a method o f e l e v a t i n g t h e i rW h i t e b l o o d c e l l c o u n t s ( l e u k o c y t e s ) . C h e m o t h e r a p e u t i ca g e n t s a r e knoWn t o e x e r t t o x i c e f f e c t s on c e r t a i n normalc e l l s i n t h e b o d y . The W h i t e b l o o d c e l l s i n t h e body t h a t a r er e s p o n s i b l e f o r h e l p i n g t h e b o d y ? g h t o f f i n f e c t i o n s a r ee s p e c i a l l y s e n s i t i v e t o c h e m o t h e r a p e u t i c a g e n t s . I f t h e s ei n f e c t i o n ? g h t i n g c e l l s , t h e W h i t e b l o o d c e l l s ) f a l l t o v e r yl o W l e v e l s i n t h e p a t i e n t r e c e i v i n g c h e m o t h e r a p y , t h e p a t i e n tW i l l become more s u s c e p t i b l e t o s e r i o u s i n f e c t i o n . TPA a sshoWn h e p r o p e n s i t y t o h e l p s p e e d t h e r a p i d r e c o v e r y o f t h ei n f e c t i o n ? g h t i n g c e l l s , b o t h a f t e r a n d d u r i n g c h e m o t h e r a p yt r e a t m e n t a n d t h e r e f o r e TPA s e s p e c i a l l y u s e f u l i n r e d u c i n gt h e c h a n c e s o f a p a t i e n t d e v e l o p i n g s e r i o u s i n f e c t i o n s . O f t e nt h e e l e v a t i o n of h e White bloo d c e l l count occurs W i t h i n oned a y o f t r e a t m e n t . The p r e s e n t i n v e n t i o n i s u s e f u l i n r a i s i n gt h e W h i t e b l o o d c e l l c o u n t i n p a t i e n t s u n d e r g o i n g chemot h e r a p y f o r a l l t y p e s o f s o l i d t u m o r s s u c h a s b r e a s t , l u n g ,p r o s t a t e a n d c o l o n c a n c e r s . TPA e l p s t o m a i n t a i n a d e q u a t el e v e l s o f W h i t e b l o o d c e l l s o r i n f e c t i o n ? g h t i n g c e l l s . Thesec e l l s Work b y s u r r o u n d i n g a n d d e s t r o y i n g b a c t e r i a t h a t mayh a v e e n t e r e d t h e b o d y . T P A , b y p r e v e n t i n g t h e n u m b e r o fW h i t e b l o o d c e l l s f r o m f a l l i n g t o loW l e v e l s f o r l o n g p e r i o d so f t i m e , l e s s e n s t h e p o t e n t i a l f o r i n f e c t i o n , t h e u s e o f

    10

    1 5

    20

    25

    30

    35

    40

    45

    50

    55

    60

    4B e c a u s e o f t h e a b i l i t y t o e l e v a t e t h e W h i t e b l o o d c e l l

    c o u n t , t h e p r e s e n t i n v e n t i o n may a l s o b e u s e f u l i n a n yp a t i e n t W i t h c o m p r o m i s e d W h i t e b l o o d c o u n t s i n c l u d i n gp a t i e n t s s u f f e r i n g f r o m A I D S .A l s o , compounds o f t h e F o r m u l a t a b o v e i n a n i m a l s t u d i e s

    h a v e e v i d e n c e d t h e a b i l i t y t o i n h i b i t s o l i d t u m o r g r o W t h i nl a b o r a t o r y a n i m a l s .D o s a g e d e l i v e r y s y s t e m s , p r e f e r a b l y a q u e o u s d o s a g e

    d e l i v e r y s y s t e m s , s u i t a b l e f o r p a r e n t e r a l a d m i n i s t r a t i o n o fcompounds o f t h e F o r m u l a I i n a p h a r m a c e u t i c a l a c c e p t a b l ec a r r i e r , c a n b e p r e p a r e d b y d i s s o l v i n g a Compound o f t h eFormula I i n a n a p p r o p r i a t e so l v e n t Which i s m i s c i b l e ,d i s p e r s a b l e o r s o l u b l e With W a t e r , such a s an a l c o h o l e g .e t h a n o l , p r o p a n o l , i s o p r o p a n o l a n d t h e l i k e . O t h e r W a t e rs o l u b l e s o l v e n t s s u i t a b l e f o r t h e p u r p o s e o f t h e p r e s e n ti n v e n t i o n i n c l u d e g l y c o l s s u c h a s p r o p y l e n e g l y c o l o r p o l ye t h y l e n e g l y c o l , g l y c e r i n e g l y c e r o l ) , g l y c e r o l f o r m a l a n d t h el i k e . There c a n b e added t o t h e dosage forms a n t i m i c r o b i a lp r e s e r v a t i v e s s u c h a s b e n Z y l a l c o h o l , p h e n o l , c r e s o l o r t h o ,meta o r p a r a o r m i x t u r e s o f t h e f o r e g o i n g ) and p h e n y l e t h yl a l c o h o l . There can a l s o be added loW c o n c e n t r a t i o n s o fs u r f a c t a n t s knoWn t o be s u i t a b l e f o r i n t r a v e n o u s use a t loWc o n c e n t r a t i o n s i n c l u d i n g E m u l p h o r E L - 6 2 0 , C r e m o p h o r E L ,P o l y s o r b a t e 8 0 ( T W e e n 8 0 ) o r P o l y s o r b a t e 2 0 ( T W e e n 2 0 ) .Any s o l v e n t o r m i x t u r e s o f s ol v e n t s a n d / o r p r e s e r v a t i v ea n d / o r s u r f a c t a n t c a n b e s e l e c t e d by t h e p e r s o n s k i l l e d i n t h ea r t a s t h e p h a r m a c e u t i c a l l y a c c e p t a b l e c a r r i e r i n a c c o r d a n c eW i t h c o n v e n t i o n a l p r a c t i c e s f o r p r e p a r i n g p a r e n t e r a l d o s a g ef o r m u l a t i o n s . A l l t h a t i s r e q u i r e d o f a component o f t h ep h a r m a c e u t i c a l l y a c c e p t a b l e c a r r i e r t o b e s u i t a b l e f o r t h ep u r p o s e s o f t h e p r e s e n t i n v e n t i o n i s t h a t i t b e s a f e Wheni n j e c t e d i n t o a human; i s m i s c i b l e , d i s p e r s i b l e o r s o l u b l e i nW a t e r ; h a s n o c y t o t o x i c i t y ; a n d d o e s n o t d i m i n i s h t h e s h e l fl i f e o f t h e p h a r m a c e u t i c a l f o r m u l a t i o n s o t h a t i t may b es t o r e d .

    The compounds o f t h e Formula I i n t h e t r e a t m e n t o fn e o p l a s t i c d i s e a s e s s u c h a s l e u k e m i a o r f o r r a i s i n g W h i t eb l o o d c e l l c o u n t s c a n b e a d m i n i s t e r e d p a r e n t e r a l l y (IV) i nd o s a g e amounts from a b o u t 0 . 0 0 1 mg e r d o s e t o a b o u t 1 . 5mg e r d o s e , f o r a b o u t 17 t i m e s p e r Week f o r a b o u t 110W e e k s ; more p r e f e r a b l e from a b o u t 0 . 0 5 t o a b o u t 1 mg , 17t i m e s p e r W e e k , f o r 1 7 W e e k s ; a n d s t i l l more p r e f e r a b l yfrom a b o u t 0 . 1 mg o a b o u t 0 . 6 mg , 17 t i m e s p e r Week f o ra b o u t 1 7 W e e k s . The m o s t p r e f e r r e d d o s a g e f o r m i s d e l i ve r e d t h r o u g h I . V . i n f u s i o n and c o n t a i n s 0 . 1 mg , 0 . 2 5 mg r0 . 5 mg e r d o s e . The c o u r s e o f t h e r a p y p r e f e r r e d i s 1 7Weeks W i t h 1 mg e i n g a d m i n i s t e r e d o v e r a Week i n d i v i d e dd o s e s .

    I n p a t i e n t s r e c e i v i n g c h e m o t h e r a p y f o r s o l i d t u m o r s , t h emost p r e f e r r e d t i m e f o r a d m i n i s t r a t i n g a s i n g l e d o s e o f acompound o f t h e F o r m u l a I i s a b o u t t h e t i m e t h e p a t i e n t i st o r e c e i v e o r h a s j u s t undergone a c o u r s e o f chemotherapyd e s i g n e d t o combat t h e s o l i d t u m o r s .

    The p r e c i s e d o s a g e a m o u n t a n d t h e d u r a t i o n o f a d m i n i st r a t i o n o f a compound o f t h e Formula W i l l depend on t h ee x i g e n c i e s o f t h e m e d i c a l s i t u a t i o n a n d t h e j u d g e m e n t o f t h ep h y s i c i a n t r e a t i n g t h e p a t i e n t i n a c c o r d a n c e W i t h c o n v e nt i o n a l p r a c t i c e among m e d i c a l p r o f e s s i o n a l s . The e x a c t d o s eW i l l depend upon s u c h f a c t o r s a s t h e a g e , W e i g h t a n dc o n d i t i o n o f t h e p a t i e n t , t h e f r e q u e n c y o f a d m i n i s t r a t i o n a n dt h e manner i n Which t h e p a t i e n t r e s p o n d s t o t h e t r e a t m e n t .

    EXAMPLEThe f o l l o W i n g compounds a r e i l l u s t r a t i v e o f t h e c o m

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    6 , 0 6 3 , 8 1 41 ) P h o r b o l 1 3 - B u t y r a t e2 ) P h o r b o l 1 2 - D e c a n o a t e3 ) P h o r b o l 1 3 - D e c a n o a t e4 ) P h o r b o l 1 2 , 1 3 - D i a c e t a t e5 ) P h o r b o l 1 3 , 2 0 - D i a c e t a t e6 ) P h o r b o l 1 2 , 1 3 - D i b e n Z o a t e7 ) P h o r b o l 1 2 , 1 3 - D i b u t y r a t e8 ) P h o r b o l 1 2 , 1 3 - D i d e c a n o a t e9 ) P h o r b o l 1 2 , 1 3 - D i h e x a n o a t e1 0 ) P h o r b o l 1 2 , 1 3 - D i p r o p i o n a t e1 1 ) P h o r b o l 1 2 - M y r i s t a t e1 2 ) P h o r b o l 1 3 - M y r i s t a t e1 3 ) P h o r b o l 1 2 - M y r i s t a t e - 1 3 - A c e t a t e a l s o known s TPA

    o r PMA1 4 ) P h o r b o l 1 2 , 1 3 , 2 0 - T r i a c e t a t e1 5 ) 1 2 - D e o x y p h o r b o l 1 3 - A n g e l a t e1 6 ) 1 2 - D e o x y p h o r b o l 1 3 - A n g e l a t e 2 0 - A c e t a t e1 7 ) 1 2 - D e o x y p h o r b o l 1 3 - I s o b u t y r a t e1 8 ) 1 2 - D e o x y p h o r b o l 1 3 - I s o b u t y r a t e - 2 0 - A c e t a t e1 9 ) 1 2 - D e o x y p h o r b o l 1 3 - P h e n y l a c e t a t e2 0 ) 1 2 - D e o x y p h o r b o l 1 3 - P h e n y l a c e t a t e 2 0 - A c e t a t e2 1 ) 1 2 - D e o x y p h o r b o l 1 3 - T e t r a d e c a n o a t e2 2 ) P h o r b o l 1 2 - T i g l i a t e 1 3 - D e c a n o a t e2 3 ) 1 2 - D e o x y p h o r b o l 1 3 - A c e t a t e2 4 ) P h o r b o l 1 2 - A c e t a t e2 5 ) P h o r b o l 1 3 - A c e t a t e

    EXAMPLEF o r m u l a t i o n T y p e A

    0 . 1 0 , 0 . 1 2 5 , 0 . 2 5 o r 0 . 5 mg f TPA a s d i s s o l v e d i n 1 . 3m l 95100 U . S . P . e t h a n o l a n d 0 . 7 m l a l i n e . Under t e r i l ec o n d i t i o n s , TPA as ? r s t d i s s o l v e d i n e t h a n o l , t h e n s a l i n eWas a d d e d , m i x e d v i g o r o u s l y , b a c t e r i o l o g i c a l l y ? l t e r e d , a n ds t o r e d i n s e a l e d s t e r i l e amber v i a l s c o n t a i n i n g e i t h e r 0 . 1 0m g / 2 m l , 0 . 1 2 5 m l / 2 ml o r 0 . 2 5 m g / 2 m l , 0 . 5 m g / 2 m l .F o r m u l a t i o n T y p e B

    0 . 1 0 , 0 . 1 2 5 , 0 . 2 5 o r 0 . 5 mg f TPA a s d i s s o l v e d i n 0 . 2ml o f e t h a n o l , 1 . 2 ml o f i s o p r o p a n o l a n d 0 . 6 m l a l i n e . Unders t e r i l e c o n d i t i o n s , TPA as ? r s t d i s s o l v e d i n t h e e t h a n o l andi s o p r o p a n o l , t h e n s a l i n e Was a d d e d , and t h e m i x t u r e Wasv i g o r o u s l y m i x e d , b a c t e r i o l o g i c a l l y ? l t e r e d a n d s t o r e d i ns e a l e d s t e r i l e a m b e r v i a l s c o n t a i n i n g e i t h e r 0 . 1 0 m g / 2 m l ,0 . 1 2 5 m l / 2 ml o r 0 . 2 5 mg/2 ml o r 0 . 5 mg/2 m l .

    A n a l y t i c a l r e s u l t s shoWed t h a t t h e r e i s no c h e m i c a lc h a n g e i n t h e TPA s o l u t i o n s s t o r e d i n t h e d a r k a t c o l dt e m p e r a t u r e f o r up t o one y e a r ; a l s o , t h e r e i s no chemicalchange i n t h e TPA o l u t i o n s s t o r e d i n t h e d a r k a t r o o mt e m p e r a t u r e up t o tWo m o n t h s .

    EXAMPLE1 . E f f e c t o f TPA n a Human P r o m y e l o c y t i c Leukemia C e l lL i n e ( H L - 6 0 ) :

    HL-60 c e l l s a t 2 > < 1 0 6 c e l l s / m l Were t r e a t e d W i t h TPA. The? n a l c o n c e n t r a t i o n s o f TPA ere 1 0 , 2 0 , o r 100 n g / m l . Thee t h a n o l c o n t e n t Was l e s s t h a n 0.01 . A f t e r 3 h o u r s o f TPAt r e a t m e n t , t h e c e l l s s t o p p e d p r o l i f e r a t i n g a n d c e l l a g g r e g at i o n and a t t a c h m e n t t o t h e d i s h Were o b s e r v e d . A f t e r 48 h o ft r e a t m e n t , t h e r e W e r e m o r p h o l o g i c a l c h a n g e s . A f t e r 4 6

    10

    20

    25

    30

    35

    40

    45

    55

    60

    6EXAMPLE 42 ) TPA+LoW D o s e s o f A r a - C :

    The t r e a t m e n t o f HL-60 c e l l s W i t h loW d o s e s o f TPA 2 0n g / m l ) o r A r a - C ( 1 0 0 n g / m l ) d e m o n s t r a t e d t h a t A r a - C c o u l di n d u c e c e l l d i f f e r e n t i a t i o n , a n d TPA t loW c o n c e n t r a t i o n i sa Weak e l l d i f f e r e n t i a t i o n - i n d u c i n g a g e n t . The c o m b i n a t i o nt r e a t m e n t o f HL-60 c e l l s W i t h TPA nd Ara-C i n d u c e d t h eHL-60 c e l l s t o d i f f e r e n t i a t e s y n e r g i s t i c a l l y .

    EXAMPLEE f f e c t o f TPA n M i c e I n j e c t e d W i t h S 1 8 0 ( S a r c o m a 1 8 0 )Tumor C e l l s :E i g h t g r o u p s o f KWen-Ming m i c e c o n t a i n i n g 7 m i c e p e r

    g r o u p Were u s e d i n t h e f o l l o W i n g e x p e r i m e n t . TWo g r o u p sWere u n t r e a t e d a n d s i x g r o u p s r e c e i v e d t h e d r u g .

    Each KWen-Ming mouse Was i n j e c t e d W i t h 5 > < 1 0 6 S180c e l l s a t t h e u n d e r - a r m p o s i t i o n . A f t e r 24 o r 72 , t h e a n i m a l sWere g i v e n TPA . p . o r l o c a l l y a t t h e t u m o r s i t e . The i n j e c t e dd o s e s o f TPA e r e 5 0 , 1 0 0 a n d 200 g / k g / d f o r 7 d a y s . Thea n i m a l s Were s a c r i ? c e d 2 4 h r s a f t e r t h e ? n a l TPA r e a t m e n tand t h e tumors Were Weighed t o c a l c u l a t e t h e e x t e n t o f t h et u m o r g r o W t h i n h i b i t i o n . The s t u d y shoWed t h a t t h e t u m o rg r o W t h Was i n h i b i t e d by 4 1 . 7 , 54.8 a n d 3 0 . 4 ,r e s p e c t i v e l y , i n m i c e t h a t W e r e i n j e c t e d ip W i t h 5 0 , 1 0 0 o r2 0 0 p i g / k g TPA d a i l y f o r 7 d a y s . The t u m o r g r o W t h Wasi n h i b i t e d b y 3 5 . 5 , 4 9 . 3 a n d 5 9 . 2 , r e s p e c t i v e l y , i n m i c et h a t W e r e i n j e c t e d d a i l y f o r 7 d a y s W i t h 5 0 , 1 0 0 o r 200 i g / k gTPA l o c a l l y a t t h e tumor s i t e i n c o m p a r i s o n t o t h e c o n t r o lm i c e . P a t h o l o g i c a l s t u d i e s shoWed t h a t t h e tumor c e l l s Wered i f f e r e n t i a t e d a f t e r t h e TPA r e a t m e n t .

    EXAMPLEE f f e c t o f TPA n Mice I n j e c t e d W i t h B16 Tumor C e l l s :

    F o u r g r o u p s o f C57 m i c e W e r e u s e d i n t h e e x p e r i m e n t .Each group c o n t a i n e d 7 mice and one group Was u n t r e a t e d .Each C57 mouse Was i n j e c t e d W i t h 0 . 2 m l o f s u p e r n a t a n t o fa 1 : 6 W/v homogenate o f B16 e l l s a t t h e u n d e r - a r m p o s i t i o n .On t h e t h i r d d a y , e a c h t r e a t m e n t g r o u p Was g i v e n TPA . p .a t 5 0 , 100 o r 200 p g / k g / d f o r 8 d a y s . The a n i m a l s Weres a c r i ? c e d a f t e r t h e t r e a t m e n t , t h e tumors Were W e i g h e d , a n dt h e r a t e s o f i n h i b i t i o n o f tumor groWth Were 40.0 , 59.4a n d 3 2 . 1 , r e s p e c t i v e l y , W h i c h W e r e a l l s t a t i s t i c a l l y d i f f e re n t from t h e c o n t r o l g r o u p .

    EXAMPLEE f f e c t o f TPA on t h e P e r i p h e r a l White Blood C e l l s (WBC)a n d H e m o g l o b i n ( H b ) C o u n t s i n S 1 8 0 C e l l - I n j e c t e d M i c e :S180 c e l l s Were i n j e c t e d i n t o m i c e . On t h e t h i r d d a y , t h em i c e Were g i v e n TPA . p . a t 5 0 , 1 0 0 o r 200 p g / k g / d f o r 7d a y s . On t h e s e c o n d d a y a f t e r t h e t r e a t m e n t Was c o m p l e t e d ,b l o o d s a m p l e s Were t a k e n from t h e t a i l s o f t h e t r e a t e d micef o r WBC n d Hb a n a l y s e s . The WBC o u n t s f o r t h e t r e a t e dg r o u p s ( 5 0 , 1 0 0 , o r 2 0 0 u g / k g / d f o r 7 d ) W e r e 1 6 1 1 7 . 4 ,1 8 7 1 3 . 0 a n d 2 0 . 7 1 3 . 4 > < 1 0 9 / L , r e s p e c t i v e l y ; t h e WBC o u n tf o r t h e c o n t r o l g r o u p Was 1 3 . 6 1 1 . 8 > < 1 0 9 / L . The Hb o f t h et r e a t e d g r o u p s Were 1 3 6 1 1 1 , 149112 and 149110 g / L , a n dt h e Hb o f t h e c o n t r o l g r o u p Was 134115 g / L . The r e s u l t si n d i c a t e t h a t ip n j e c t i o n o f TPA o u l d i n c r e a s e t h e p e r i p he r a l WBC o u n t s i n mice i n a d o s e - d e p e n d e n t manner,Whereas t h e Hb l e v e l s Were n o t g r e a t l y a f f e c t e d i n TPAt r e a t e d m i c e When compared t o t h e c o n t r o l m i c e .

    EXAMPLEStudy on t h e C l i n i c a l Use o f TPA n Hum ans1 . D o s e R a n g i n g S t u d y .Due t o t h e s t r o n g l o c a l i r r i t a t i o n c a u s e d by TPA

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    6 , 0 6 3 , 8 1 47

    2 . The T o x i c i t y a n d S i d e E f f e c t s o f D i f f e r e n t TPA DosesA d m i n i s t e r e d C l i n i c a l l y :

    1 ) TPA g i v e n a t 1 m g / p a t i e n t / W e e k :One mg TPA n s o l u t i o n Was m ixed W e l l W i t h 200 m l o f

    s a l i n e f o r i v . i n f u s i o n Which Was completed i n 1 h t t h e r a t eo f 1 6 p i g / m i n . One h o u r a f t e r TPA a d m i n i s t r a t i o n , p a t i e n t ss t a r t e d t o h a v e c h i l l s Which l a s t e d f o r a b o u t 30 m i n , f o ll o W e d b y f e v e r , t h e p a t i e n t s t e m p e r a t u r e r e a c h e d3 7 . 5 3 9 . 5 C . Which l a s t e d f o r 35 h , t h e n r e t u r n e d t on o r m a l ) W i t h l i g h t t o h e a v y p e r s p i r a t i o n . T h e a b o v e sympt o m s c o u l d b e a l l e v i a t e d b y g i v i n g t h e p a t i e n t s g l u c o c o r t ic o i d s . TPA t t h i s d o s e c a u s e d a m i n o r i t y o f p a t i e n t s t ob l e e d , s e v e r a l p a t i e n t s s u f f e r e d f o r a s h o r t p e r i o d o f t i m ed i f ? c u l t y i n b r e a t h i n g , a n d Hb Was d e t e c t e d i n t h e u r i n e .HoWever, t h e s e s i d e e f f e c t s Were s h o r t l i v e d a n d r e v e r s i b l e .The c a r d i a c , h e p a t i c , r e n a l a n d p u l m o n a r y f u n c t i o n s W e r e a l lfound t o be n o r m a l .

    2 ) TPA g i v e n a t 0 . 5 m g / p a t i e n t > < 2 / W e e k : ( t W o d o s e s aW e e k )0 . 5 mg f TPA n s o l u t i o n Was miXed W e l l W i t h 200 m l o f

    s a l i n e f o r i v . i n f u s i o n Which Was completed i n 1 h t t h e r a t eo f 8 p i g / m i n . The r e a c t i o n s a f t e r a d m i n i s t r a t i o n Were s i m i l a rt o t h a t o f t h e 1 mg TPA o s a g e , b u t t o a l e s s e r e X t e n t t h a nt h e 1 mg o s e . The p a t i e n t s t o l e r a t e d t h e l o W e r d o s e moree a s i l y . O c c a s i o n a l l y , Hb Was d e t e c t e d i n p a t i e n t s u r i n e .D i f ? c u l t y i n b r e a t h i n g Was n o t o b s e r v e d . The c a r d i a c ,h e p a t i c , r e n a l a n d p u l m o n a r y f u n c t i o n s W e r e a l l n o r m a l .

    3 ) TPA g i v e n a t 0 . 2 5 m g / p a t i e n t > < 4 / W e e k :0 . 2 5 mg f TPA n s o l u t i o n Was miXed W e l l W i t h 200 m l

    o f s a l i n e f o r i v . i n f u s i o n Which Was completed i n 1 h a t t h er a t e o f 4 p i g / m i n . A f t e r t h e a d m i n i s t r a t i o n , symptoms s u c h a sc h i l l s and f e v e r Were a l s o o b s e r v e d , b u t t o a m u c h l e s s e re X t e n t t h a n W i t h t h e h i g h e r d o s a g e s . No H b Was d e t e c t e d i nt h e u r i n e , a n d n o p a t i e n t s u f f e r e d d i f ? c ul t y i n b r e a t h i n g . T h ec a r d i a c , h e p a t i c , r e n a l a n d p u l m o n a r y f u n c t i o n s W e r e a l ln o r m a l .

    A f t e r comparing t h e a b o v e t h r e e d o s a g e s , 0 . 2 5m g / p e r s o n > < 4 / W e e k a n d 0 . 5 m g / p e r s o n > < 2 / W e e k a r e c o n s i de r e d t o b e p r e f e r r e d d o s a g e s o f T P A .

    EXAMPLEThe r e s u l t s o b t a i n e d upon t r e a t m e n t o f p a t i e n t s W i t h TPA

    a s p r e s e n t e d i n t a b u l a r f o r m a n d i n s u b s e q u e n t e x a m p l e s .TABLE

    C l i n i c a lSummary o f C l i n i c a l E ? i c a c y o f TPA n t h e F i v e C a s e s

    R e p r e s e n t i n g C h r o n i c M y e l o c y t i c L e u k e m i a H a v i n g P r o g r e s s e d t o A c u t eM y e l o c y t i c L e u k e m i a B e f o r e TPA d m i n i s t r a t i o n S u b j e c t s 1 - 5 )

    a n d F i v e C a s e s o f O t h e r L e u k e m i a s ( S u b j e c t s 6 1 0 )Bone marr oW M y e l o b l a s t a n d p r o m y e l o c y t e

    S u b j e c t p e r c e n t o f t o t a l c e l l sNo. Before TPA A f t e r TPA

    1 30 2 . 52 36 3 . 03 90 2 . 04 6 7 . 5 4 . 55 2 7 . 5 1 . 56 48 37 16 108 8 0 . 8 179 A p l a s t i c a n e m i a ) ( T P A t e r m i n a t e d )

    1 0 (9 e a r l y i n TPA 0

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    8TABLE 2

    C l i n i c a lSummary o f TPA i n d u c e d W h i t e B l o o d C e l l C h a n g e s (WBC) i nP a t i e n t s W i t h S o l i d T u m o r s U n d e r g o i n g C h e m o t h e r a p y

    S u b j e c t WBC X 1 0 9 / l i t e rNo. B e f o r e TPA Peak a f t e r TPA1 1 0 . 7 6 . 812 3 . 0 4 . 513 0 . 9 2 . 514 3 . 8 8 . 015 2 . 4 7 . 116 2 . 4 5 . 217 2 . 0 4 . 418 2 . 4 4 . 019 2 . 9 5 . 120 0 . 7 2 . 721 1 . 1 1 . 52 2 1 . 9 7 . 623 2 . 3 3 . 924 1 . 1 5 . 325 2 . 1 6 . 426 3 . 6 5 . 6

    EXAMPLE 10I n t h e s u b j e c t s i d e n t i ? e d a s 1 ) t h r o u g h 5 ) b e l o W , c h r o n i cm y e l o c y t i c l e u k e m i a h a d p r o g r e s s e d t o a c u t e m y e l o c y t i c

    leukemia b e f o r e t r e a t m e n t W i t h TPA.S u b j e c t N o . 1 ) TS, m a l e , 3 2 , p a t i e n t N o . 2 8 8 7 9 . B l o o d

    p r o ? l e b e f o r e TPA r e a t m e n t : H b : 2 8 g / L ; WBC: 1 . 0 > < 1 0 9 /L , p l a t e l e t : 1 3 5 > < 1 0 9 / L . Bone mar roW p r o ? l e b e f o r e TPAt r e a t m e n t : m y e l o b l a s t + p r o m y e l o c y t e : 30 . TPA r e a tm e n t : 1 m g / W e e k ( 0 . 2 5 m g a d m i n i s t e r e d f o u r t i m e s ) f o rtWo W e e k s . Blood p r o ? l e a f t e r t r e a t m e n t : H b : 8 6 g / L ;WBC: 2 . 8 > < 1 0 9 / L , p l a t e l e t : 2 8 3 x 1 0 9 / L . Bone mar roW r o? l e a f t e r TPA r e a t m e n t : m y e l o b l a s t + p r o m y e l o c y t e : 2 . 5 .

    S u b j e c t N o . 2 ) C . J . , m a l e , 3 0 , p a t i e n t N o . 2 9 9 2 6 . D i a g n o s i s :c h r o n i c m y e l o c y t i c l e u k e m i a became a c u t e m y e l o c y t i cl e u k e m i a b e f o r e t r e a t m e n t . Blood p r o ? l e b e f o r e TPAt r e a t m e n t : H b : 9 4 g / L ; WBC: . 8 > < 1 0 9 / L , p l a t e l e t : 6 3 x 1 0 9 /L . S p l e e n : 3 cm eloW t h e r i b c a g e . Bone marro W p r o ? l eb e f o r e TPA t r e a t m e n t : m y e l o b l a s t + p r o m y e l o c y t e : 36 .TPA t r e a t m e n t : 1 mg/Week f o r 5 W e e k s . B l o o d p r o ? l ea f t e r t r e a t m e n t : H b : 1 0 4 g / L ; WBC: 4 . 9 > < 1 0 9 / L , p l a t e l e t :8 0 x 1 0 9 / L . S p l e e n : 0 . 5 c m beloW t h e r i b c a g e . Bonema rroW p r o ? l e a f t e r TPA t r e a t m e n t : myeloblast+p r o m y e l o c y t e : 3 .

    S u b j e c t N o . 3 ) Z . K . , m a l e , 4 2 , p a t i e n t N o . 1 8 1 0 2 . D i a g n os i s : c h r o n i c m y e l o c y t i c l e u k e m i a became a c u t e m y e l oc y t i c l e u k e m i a b e f o r e t r e a t m e n t . B l o o d p r o ? l e b e f o r e TPAt r e a t m e n t : H b : 7 0 g / L ; WBC: 2 7 . 5 > < 1 0 9 / L , p l a t e l e t :2 1 > < 1 0 9 / L . Bone marroW p r o ? l e b e f o r e TPA r e a t m e n t :myeloblast+promyelocyte: 90 . TPA t r e a t m e n t : 1mg/Week f o r 7 W e e k s . B l o o d p r o ? l e a f t e r t r e a t m e n t : H b :9 6 g / L ; WBC: 2 2 > < 1 0 9 / L , p l a t e l e t : 7 0 > < 1 0 9 / L . Bone m a rroW pro?le a f t e r TPA t r e a t m e n t : myeloblast+p r o m y e l o c y t e : 2 .

    S u b j e c t N o . 4 ) W . F . m a l e , 2 5 , p a t i e n t N o . 2 1 3 1 5 . D i a g n o s i s :c h r o n i c m y e l o c y t i c l e u k e m i a became a c u t e m y e l o c y t i cl e u k e m i a b e f o r e t r e a t m e n t . Blood p r o ? l e b e f o r e TPAt r e a t m e n t : H b : 8 7 g / L ; WBC: 1 9 > < 1 0 9 / L , p l a t e l e t : 1 5 0 > < 1 0 9 / L , p l a t e l e t : 2 9 0 > < 1 0 9 / L . B o n emarroW p r o ? l e b e f o r e TPA t r e a t m e n t : m y e l o b l a s t +p r o m y e l o c y t e : 2 7 . 5 . TPA t r e a t m e n t : 1 mg/Week f o r 2W e e k s . B l o o d p r o ? l e a f t e r t r e a t m e n t : H b : 84 g / L ; WBC:2 7 . 3 > < 1 0 9 / L , p l a t e l e t : 1 7 0 > < 1 0 9 / L . B o n e m a r r o W p r o ? l ea f t e r TPA r e a t m e n t : m y e l o b l a s t + p r o m y e l o c y t e : 1 . 5 .A l l t h e a b o v e p a t i e n t s h a d r e c e i v e d v a r i o u s r e g i m e n s o f

    c h e m o t h e r a p y p r i o r t o t h e TPA t r e a t m e n t , i n c l u d i n gh y d r o X y u r e a , b u s u l f a n , and A r a - C , e t c . b u t none Was e f f e ct i v e a t t h e s t a r t o f TPA r e a t m e n t . Before t h e a d m i n i s t r a t i o no f T P A , p a t i e n t s r e c e i v e d i n j e c t i o n o f 6 > < 1 0 5 u n i t s o f v i t a m i nD3 ( V D 3 ) / p e r s o n f o r 2 d a y s ; a f t e r t h e TPA d m i n i s t r a t i o n ,p a t i e n t s r e c e i v e d i v . i n f u s i o n o f 40 m g o f A r a - C / d > < 3 . A f t e rt h e t r e a t m e n t , t h e p a t i e n t s a l l a c h i e v e d c l i n i c a l r e m i s s i o n i nbone marroW p a r a m e t e r s i n a s h o r t t i m e . I n a d d i t i o n , d u r i n gand a f t e r t h e treatment, t h e r e W a s no bone m a r r o Ws u p p r e s s i o n , n o r i n f e c t i o n o r b l e e d i n g . T h e s e p a t i e n t s h a v ebeen i n c l i n i c a l r e m i s s i o n f o r o v e r 6 months.

    EXAMPLE 1O t h e r T y p e s o f L e u k e m i a :S u b j e c t N o . 6 ) YR, m a l e , 5 7 . D i a g n o s e d a s AML-M3.S y m p t o m s b e g a n i n J a n u a r y , 1 9 9 5 . B l o o d p r o ? l e : H b : 6 0g / L , WBC: 0 . 4 > < 1 0 9 / L , p l a t e l e t : 4 0 > < 1 0 9 / L . B o n e m a r r o W

    p r o ? l e : m y e l o b l a s t + p r o m y e l o c y t e : 4 8 . T h e TPA r e a tment p e r i o d Was 1 mg/Week f o r t h r e e W e e k s , a n d 6 > < 1 0 5u n i t s V D 3 / d > < 3 W e r e i n j e c t e d p r i o r t o t h e t r e a t m e n t . A f t e rt h e ? r s t t r e a t m e n t p e r i o d , b l o o d p r o ? l e : H b : 1 1 8 g / L ,WBC: 4 . 1 > < 1 0 9 / L , p l a t e l e t : 8 0 x 1 0 9 / L . Bone marro W p r o? l e : m y e l o b l a s t + p r o m y e l o c y t e : 3 , Which met t h e s t a nd a r d f o r AML-M3 r e m i s s i o n . The p a t i e n t h a s b e e n i nr e m i s s i o n a f t e r t r e a t m e n t f o r a t l e a s t 6 m o n t h s .S u b j e c t N o . 7 ) M . W . , m a l e , 6 7 . D i a g n o s i s : MDS-REABa c c o m p a n i e d b y a n i n c r e a s e d number o f m o n o c y t e s . F o u rmonths o f o r a l VP16 a d m i n i s t r a t i o n f a i l e d t o p r o d u c er e s u l t s . The p a t i e n t s t a r t e d t o r e c e i v e a c o m b i n a t i o nt r e a t m e n t o f 1 , 2 5 - ( O H ) 2 V D3 +TPA+loW d o s e A r a - C .TPA d o s a g e : 0 . 2 5 0 . 5 m g 1 m g p e r W e e k ) f o r e l e v e nW e e k s . Blood p r o ? l e b e f o r e TPA r e a t m e n t : H b : 3 6 g / L ;WBC: 4 . 0 > < 1 0 9 / L , p l a t e l e t : 2 9 > < 1 0 9 / L . M y e l o b l a s t : 2 ,P r o m y e l o c y t e : 4 , M y e l o c y t e : 3 , N e u t r o p h i l : 6 0 ,L y m p h o c y t e : 2 5 , M o n o c y t e 6 . B o n e m a r r o W p r o ? l eb e f o r e t r e a t m e n t : a c t i v e i n p r o l i f e r a t i o n , m y e l o b l a s t : 8 ,p r o m y e l o c y t e : 8 . S p l e e n : 3 c m beloW h e r i b c a g e . A f t e rt h e t r e a t m e n t : S p l e e n : 0 . 5 cm beloW t h e r i b c a g e . Bloodp r o ? l e : H b : 4 2 g / L ; WBC: 1 0 . 2 > < 1 0 9 / L , p l a t e l e t : 3 4 > < 1 0 9 /L . N e u t r o p h i l : 8 0 , L y m p h o c y t e : 1 9 , M o n o c y t e 1 .Promyelocytes Were n o t d e t e c t e d . Bone marroW p r o ? l e :a c t i v e i n p r o l i f e r a t i o n , m y e l o b l a s t : 4 , p r o m y e l o c y t e :6 .

    S u b j e c t N o . 8 ) L . Q . , m a l e , 3 6 . D i a g n o s i s : AML-M3. r e a tment W i t h r e t i n o i c a c i d (RA) a t 80 m g / d a y > < 5 0 Was n o ts u c c e s s f u l . Blood p r o ? l e b e f o r e t h e t r e a t m e n t W i t h TPA:H b : 4 5 g / L , WBC: 1 . 0 > < 1 0 9 / L , p l a t e l e t : 3 5 > < 1 0 9 / L . B o n emarroW p r o ? l e : v e r y a c t i v e i n p r o l i f e r a t i o n , m y e l o b l a s t +p r o m y e l o c y t e : 8 0 . 8 . B l o o d p r o ? l e a f t e r t h e TPA r e a tm e n t : H b : 6 6 g / L , WBC: 2 . 2 > < 1 0 9 / L , p l a t e l e t : 2 2 3 > < 1 0 9 / L .Bone mar roW p r o ? l e : a c t i v e i n p r o l i f e r a t i o n , m y e l o b l a s t +p r o m y e l o c y t e : 1 7 .

    S u b j e c t N o . 9 ) Z . H. , f e m a l e , 2 1 . D i a g n o s i s : b o n e m a r r o Ws u p p r e s s i o n a f t e r r e c e i v i n g c h e m o t h e r a p y f o r c h r o n i cm y e l o c y t i c l e u k e m i a , s e c o n d a r y a p l a s t i c a n e m i a . T h e

    1 0? l e : a p l a s t i c a n e m i a . TPA d o s a g e : 0 . 2 5 m g > < 2 . B l o o dp r o ? l e a f t e r t h e TPA r e a t m e n t : H b : 3 2 g / L , WBC: . 9 > < 1 0 9 / L . Due o s e v e r e a n e m i a , t h e TPAtreatment Was terminated.5 S u b j e c t N o . ( 1 0 ) L . N . , f e m a l e , 2 6 . D i a g n o s i s : CML. T h e

    10

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    p a t i e n t h a d b e e n t r e a t e d W i t h c h e m o t h e r a p y u s i n g t h ec o m b i n a t i o n o f h o m o h a r ri n g t o m i n e a n d A r a - C . B l o o dp r o ? l e b e f o r e TPA r e a t m e n t : H b : 9 8 g / L ; WBC: 2 . 0 > < 1 0 9 /L , p l a t e l e t : 1 0 2 x 1 0 9 / L . 0 . 2 5 m g TPA d m i n i s t e r e d t o t h ep a t i e n t o n c e . B l o o d p r o ? l e a f t e r t r e a t m e n t : H b : 9 6 g / L ;WBC: . 0 > < 1 0 9 / L , p l a t e l e t : 1 1 2 > < 1 0 9 / L . On h e s e c o n d d a ya f t e r TPA t r e a t m e n t : m y e l o b l a s t + p r o m y e l o c y t e : 4 ,m y e l o c y t e 5 . On t h e ? f t h d a y a f t e r t h e TPA r e a t m e n t ,t h e s e t y p e s o f b l o o d c e l l s c o m p l e t e l y d i s a p p e a r e d .

    EXAMPLE 12P a t i e n t s u n d e r g o i n g c h e m o t h e r a p y f o r t h e t r e a t m e n t o f

    s o l i d t u m o r s .S u b j e c t N o . 1 1 ) L . X . , f e m a l e , 5 0 . D i a g n o s i s : m a l i g n a n tl y m p h o m a . T h e p a t i e n t h a d r e c e i v e d a d r a m y c i n ,v i n c r i s t i n e , a n d hormonal t r e a t m e n t . The b l o o d c e l lc o u n t s Were d e c r e a s e d t o : H b : 78 g / L , WBC: . 7 > < 1 0 9 / L ,p l a t e l e t : 245 < 1 0 9 / L . 0 . 2 5 mg TPA as a d m i n i s t e r e d t o t h ep a t i e n t 4 t i m e s . The b l o o d c e l l c o u n t s improved o : H b : 7 6g / L , WBC: 6 . 8 > < 1 0 9 / L , p l a t e l e t : 3 3 1 x 1 0 9 / L . Chemot h e r a p y Was t h e n c o n t i n u e d f o r 5 more d a y s , a n d f o l l o W e dby one dose o f 0 . 5 mg TPA. The WBC count Wasm a i n t a i n e d a t 3 . 0 > < 1 0 9 / L . T h e p a t i e n t i s s t i l l r e c e i v i n gtreatment.

    S u b j e c t N o . 1 2 ) Y . G . , f e m a l e , 4 5 . D i a g n o s i s : b r a i n t u m o r .B l o o d p r o ? l e a f t e r c h e m o t h e r a p y W a s : H b : 1 1 9 g / L ,WBC: 3 . 0 > < 1 0 9 / L , p l a t e l e t : 3 9 9 x 1 0 9 / L . 0 . 2 5 m g TPA Wasg i v e n t o t h e p a t i e n t o n c e . On t h e d a y a f t e r t h e TPAt r e a t m e n t , t h e b l o o d p r o ? l e Was H b : 1 2 3 g / L , WBC:4 . 5 > < 1 0 9 / L , p l a t e l e t : 4 3 6 x 1 0 9 / L . T h e p a t i e n t r e c e i v e d f u rt h e r c h e m o t h e r a p y .

    S u b j e c t N o . 1 3 ) G . F . , m a l e , 6 0 . D i a g n o s i s : l u n g c a n c e r .A f t e r c h e m o t h e r a p y , h i s blood e l l c o u n t s Were d e c r e a s e dt o : H b : 7 6 g / L , WBC: 0 . 9 > < 1 0 9 / L , p l a t e l e t : 1 0 0 > < 1 0 9 / L .0 . 2 5 mg TPA Was g i v e n t o t h e p a t i e n t t W i c e . On t h e d a ya f t e r t h e TPA t r e a t m e n t , H b : 7 4 g / L , WBC: 2 . 5 > < 1 0 9 / L ,p l a t e l e t : 1 1 0 > < 1 0 9 / L . T h e p a t i e n t i s s t i l l r e c e i v i n g t r e a tment.

    S u b j e c t N o . ( 1 4 ) Z . R . , f e m a l e , 4 4 . D i a g n o s i s : b r e a s t c a n c e r .The WBC f t e r c h e m o t h e r a p y Was 3 . 8 > < 1 0 9 / L . 0 . 2 5 mg fTPA a s g i v e n t o t h e p a t i e n t o n c e . The WBC n t h e daya f t e r t h e TPA r e a t m e n t Was 8 . 0 > < 1 0 9 / L .

    S u b j e c t N o . 1 5 ) OZ, f e m a l e , 7 5 . D i a g n o s i s : E s o p h a g e a lC a n c e r . S u r g e r y Was p e r f o r m e d , f o l l o W e d b y chemot h e r a p y u s i n g c i s p l a t i n , 5 - ? u o r o u r a c i l . B l o o d p r o ? l eb e f o r e T P A ) : WBC: 2 . 4 > < 1 0 9 / L ; n e u t r o p h i l : 8 3 , l y mp h o c y t e : 1 7 ; p l a t e l e t : 1 5 0 > < 1 0 9 / L ; R B C : 3 . 4 3 > < 1 0 1 2 / L ;H b : 1 0 7 g / L . TPA o s a g e : 0 . 25 m g . B l o o d p r o ? l e ( o n e d a ya f t e r T P A ) : WBC: 7 . 1 > < 1 0 9 / L ; n e u t r o p h i l : 9 4 ; l y m p h oc y t e : 6 ; p l a t e l e t : 7 7 > < 1 0 9 / L ; R B C : 3 . 3 3 > < 1 0 1 2 / L ; H b : 1 0 9g / L . B l o o d p r o ? l e 4 d a y s a f t e r T P A ) : WBC: 4 . 4 > < 1 0 9 / L ;n e u t r o p h i l : 9 7 ; l y m p h o c y t e : 3 ; p l a t e l e t : 1 0 5 > < 1 0 9 / L ;RBC: 3 . 3 6 > < 1 0 1 2 / L , H b : 1 1 2 g / L . Symptoms a f t e r T P A :C h i l l , f e v e r , l o c a l i r r i t a t i o n a n d s l i g h t h e a d a c h e . T h ec a r d i a c , h e p a t i c , r e n a l a n d p u l m o n a r y f u n c t i o n s W e r en o r m a l .

    S u b j e c t N o . 1 6 ) X . H . , f e m a l e , 6 0 . D i a g n o s i s : E s o p h a g e a lC a n c e r . S u r g e r y Was p e r f o r m e d , f o l l o W e d b y chemot h e r a p y u s i n g V P 1 6 , MTX, MMC n d c i s p l a t i n . TPA

  • 8/13/2019 Biosuccess Biotech v. Rich Pharmaceuticals et. al.

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    6 , 0 6 3 , 8 1 41 1o n e d a y a f t e r T P A ) : WBC: 5 . 2 > < 1 0 9 / L ; n e u t r o p h i l : 8 7 ;l y m p h o c y t e : 1 3 ; p l a t e l e t 6 0 x 1 0 9 / L ; R B C : 3 . 7 6 > < 1 0 1 2 / L ;H b : 1 2 2 g / L . B l o o d p r o ? l e ( 2 d a y s a f t e r TPAa d m i n i s t r a t i o n ) . WBC: 4 . 5 > < 1 0 9 / L ; n e u t r o p h i l 8 0 ; l y mp h o c y t e : 2 0 ; p l a t e l e t : 6 4 > < 1 0 9 / L ; R B C : 2 . 9 9 > < 1 0 1 2 / L ;

    H b : 1 0 9 g / L . Symptoms a f t e r T P A : c h i l l s , f e v e r , l o c a li r r i t a t i o n , c a r d i a c , h e p a t i c , r e n a l a n d p u l m o n a r y W e r en o r m a l .

    S u b j e c t N o . 1 7 ) Y . Z . , f e m a l e , 3 7 . D i a g n o s i s : b r e a s t c a n c e r .S u r g e r y W a s p e r f o r m e d , f o l l o W e d b y c h e m o t h e r a p y u s i n gCTX, MTX, a n d 5 - F U . TPA d o s e : 0 . 2 5 m g > < 2 ( S e c o n dd o s e Was 4 d a y s a f t e r 1 s t d o s e ) . B l o o d p r o ? l e ( b e f o r eT P A ) : WBC: 2 . 0 > < 1 0 9 / L ; n e u t r o p h i l : 8 5 ; l y m p h o c y t e :1 5 ; p l a t e l e t : 1 0 6 x 1 0 9 / L ; R B C : 3 . 2 4 > < 1 0 1 2 / L ; H b : 1 0 7g / L . B l o o d p r o ? l e 3 d a y s a f t e r ? r s t TPA o s e ) : WBC:2 . 9 > < 1 0 9 / L ; n e u t r o p h i l 8 3 ; l y m p h o c y t e : 1 7 ; p l a t e l e t :1 2 2 > < 1 0 9 / L ; RBC: 3 . 3 6 > < 1 0 1 2 / L ; H b : 1 0 7 g / L . B l o o d p r o? l e 2 d a y s a f t e r s e c o n d TPA o s e ) : WBC: 3 . 8 > < 1 0 9 / L ;n e u t r o p h i l : 8 4 ; l y m p h o c y t e : 1 6 ; p l a t e l e t : 8 4 x 1 0 9 / L ;R B C : 3 . 4 7 > < 1 0 1 2 / L . B l o o d p r o ? l e 4 d a y s a f t e r s e c o n dTPA o s e ) : WBC: 4 . 4 > < 1 0 9 / L ; n e u t r o p h i l : 8 6 ; l y m p h oc y t e : 1 4 ; p l a t e l e t 1 9 3 > < 1 0 9 / L ; R B C : 3 . 4 9 > < 1 0 1 2 / L ; H b :1 1 2 g / L . Symptoms a f t e r T P A : p a t i e n t s t a r t e d t o h a v ec h i l l s W h i c h l a s t e d f o r 2 h r s f o l l o W e d by f e v e r , t e m p e r at u r e r e a c h e d 3 8 C . Which l a s t e d 4 h r s and l o c a l i r r i t a t i o n .T h e c a r d i a c , h e p a t i c , r e n a l a n d p u l m o n a r y f u n c t i o n s W e r en o r m a l .

    S u b j e c t N o . ( 1 8 ) HR, m a l e , 5 6 . D i a g n o s i s : C o l o n c a n c e r .S u r g e r y W a s p e r f o r m e d , f o l l o W e d b y c h e m o t h e r a p y u s i n gC i s p l a t i n , V P 1 6 , a n d 5 - F U . TPA o s e : 0 . 2 5 m g > < 2 ( 2 n dTPA o s e Was a d m i n i s t e r e d 24 h r s a f t e r 1 s t TPA o s e ) .B l o o d p r o ? l e b e f o r e T P A ) : WBC: . 4 > < 1 0 9 / L ; n e u t r o p h i l :6 3 ; l y m p h o c y t e : 3 7 ; p l a t e l e t : 2 0 8 x 1 0 9 / L ; R B C : 4 . 0 x1 0 1 2 ; H b : 1 0 4 g / L . B l o o d p r o ? l e ( o n e d a y a f t e r 2 n d TPAd o s e ) : WBC: 4 . 0 > < 1 0 9 / L ; n e u t r o p h i l : 6 0 ; l y m p h o c y t e :4 0 ; p l a t e l e t : 1 9 8 x 1 0 9 / L ; R B C : 4 . 1 > < 1 0 1 2 ; H b : 1 1 2 g / L .Symptoms a f t e r T P A : c h i l l s , f e v e r , l o c a l i r r i t a t i o n . C a rd i a c h e p a t i c , r e n a l a n d p u l m o n a r y f u n c t i o n s W e r e n o r m a l .

    S u b j e c t N o . 1 9 ) Z . T . , m a l e , 6 6 . D i a g n o s i s : l u n g c a n c e rm e t a s t a s i Z e d t o a d r e n a l g l a n d . S u r g e r y Was p e r f o r m e d ,f o l l o W e d b y c h e m o t h e r a p y u s i n g MMC, VCR, a n d C T X .TPA o s a g e : 0 . 2 5 m g > < 2 ( 2 n d TPA o s e Was a d m i n i s t e r e d2 4 h r s a f t e r 1 s t TPA o s e ) . B l o o d p r o ? l e b e f o r e T P A ) :WBC: 2 . 9 > < 1 0 9 / L ; n e u t r o p h i l : 7 6 ; l y m p h o c y t e : 2 4 ;p l a t e l e t : 2 2 7 > < 1 0 9 / L ; R B C : 3 . 3 3 > < 1 0 1 2 / L ; H b : 1 0 0 g / L .B l o o d p r o ? l e ( o n e d a y a f t e r s e c o n d TPA o s e ) : WBC:5 . 1 > < 1 0 9 / L ; n e u t r o p h i l : 8 2 ; l y m p h o c y t e : 1 8 ; p l a t e l e t :N / A ; RBC: N / A ; H b : 9 3 g / L . B l o o d p r o ? l e 2 d a y s a f t e r2 n d TPA o s e ) : WBC: 5 . 0 > < 1 0 9 / L ; n e u t r o p h i l : 8 0 ; l y mp h o c y t e : 2 0 ; p l a t e l e t : N / A ; R B C : 3 . 2 5 > < 1 0 1 2 ; H b : 1 0 1g / L . Symptoms a f t e r T P A : c h i l l s , f e v e r , l o c a l i r r i t a t i o n .C a r d i a c , h e p a t i c r e n a l a n d p u l m o n a r y f u n c t i o n s W e r en o r m a l .S u b j e c t N o . 2 0 ) J . Z . , m a l e , 6 8 . D i a g n o s i s : e s o p h a g e a lc a n c e r m e t a s t a s i Z e d t o l i v e r , l u n g a n d b r a i n . The p a t i e n tr e c e i v e d c h e m o t h e r a p y u s i n g T a X o l , c i s p l a t i n , 5 - F U a n dS e m u s t i a l . T o t a l TPA o s a g e : 2 m g . B l o o d p r o ? l e b e f o r eT P A ) : WBC: 0 . 7 > < 1 0 9 / L ; n e u t r o p h i l : 2 9 ; l y m p h o c y t e :7 1 ; p l a t e l e t : N / A ; RBC: 2 . 8 2 > < 1 0 1 2 / L ; H b : 8 7 g / L . TPAt r e a t m e n t s c h e d u l e . On t h e ? r s t a n d t h i r d d a y , 0 . 2 5 mgWas g i v e n a n d on t h e 5 t h , 7 t h a n d 9 t h d a y 0 . 5 mg f TPAW a s g i v e n . B l o o d p r o ? l e a t d a y 2 ) : WBC: 0 . 9 > < 1 0 9 / L ;n e u t r o p h i l : 6 6 ; l y m p h o c y t e : 3 4 ; p l a t e l e t : 8 2 x 1 0 9 / L ;R B C : 2 . 1 7 > < 1 0 1 2 / L ; H b : 7 2 g / L . B l o o d p r o ? l e a t d a y 4 ) :

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    1 2l y m p h o c y t e : 5 ; p l a t e l e t : 4 3 > < 1 0 9 / L ; R B C : 1 . 9 > < 1 0 1 2 ; H b :7 0 g / L . B l o o d p r o ? l e a t d a y 8 ) : WBC: 2 . 3 > < 1 0 9 / L ; n e ut r o p h i l : 9 1 ; l y m p h o c y t e : 9 ; p l a t e l e t : 9 0 > < 1 0 9 / L ; R B C :1 . 7 1 > < 1 0 1 2 / L ; H b : 6 1 g / L . B l o o d p r o ? l e a t 1 1 t h d a y ) :WBC: 2 . 7 > < 1 0 9 / L ; n e u t r o p h i l : 8 5 ; l y m p h o c y t e : 1 5 ;p l a t e l e t : 3 7 . 6 > < 1 0 9 / L ; R B C : 2 . 9 1 > < 1 0 1 2 / L ; H b : 6 1 g / L .B l o o d p r o ? l e a t d a y 1 3 ) : WBC: 1 . 9 > < 1 0 9 / L ; n e u t r o p h i l :9 0 ; l y m p h o c y t e : 1 0 ; p l a t e l e t : 3 2 > < 1 0 9 / L ; R B C : 1 . 7 3 > < 1 0 9 / L ; n e u t r o p h i l :7 3 ; l y m p h o c y t e : 2 7 ; p l a t e l e t : 1 4 4 > < 1 0 9 / L ; R B C : 4 . 1 5 > < 1 0 9 / L ; n e u t r o p h i l : N / A ; l y m p h o c y t e : N / A ; p l a t e l e t :6 9 > < 1 0 9 / L ; RBC: 4 . 1 5 > < 1 0 1 2 / L : H b : 1 1 7 g / L . B l o o d p r o ? l ea t d a y 4 ) : WBC: 0 . 6 > < 1 0 9 / L ; n e u t r o p h i l : 2 8 ; l y m p h oc y t e : 7 2 ; p l a t e l e t : 6 8 x 1 0 9 / L ; R B C : 3 . 9 5 > < 1 0 1 2 / L ; H b :1 0 9 g / L . B l o o d p r o ? l e a t d a y 7 ) : WBC: 0 . 8 > < 1 0 9 / L ;n e u t r o p h i l : 8 8 ; l y m p h o c y t e : 1 2 ; p l a t e l e t : 6 0 > < 1 0 9 / L ;R B C : 4 . 2 2 > < 1 0 1 2 / L ; H b : 1 1 0 g / L . B l o o d p r o ? l e a t d a y 9 ) :WBC: 1 . 5 > < 1 0 9 / L ; n e u t r o p h i l : 8 0 ; l y m p h o c y t e : 2 ;p l a t e l e t : 6 9 > < 1 0 9 / L ; R B C : 4 . 0 2 > < 1 0 1 2 / L ; H b : 1 1 2 g / L .Symptoms a f t e r T P A : No c h i l l s a n d f e v e r , o n l y l o c a li r r i t a t i o n . C a r d i a c , h e p a t i c , r e n a l a n d p u l m o n a r y f u n c t i o n ssame a s b e f o r e TPA r e a t m e n t . S i n c e t h i s p a t i e n t s lymp ho m a c e l l s h a d m e t a s t a s i Z e d t o t h e b o n e m a r r o W , s h er e q u i r e d a h i g h d o s e o f TPA 2 . 5 mg) a n d a l o n g e rt r e a t m e n t t i m e ( 9 d a y s ) i n o r d e r t o i n d u c e a v e r y loW l e v e lo f WBC.S u b j e c t N o . 2 2 ) X . Y . , f e m a l e , 3 4 . D i a g n o s i s : N a s o p h a r y ng e a l c a r c i n o m a m e t a s t a s i Z e d t o n e c k lymph n o d e . Thep a t i e n t Was r e a t e d W i t h c h e m o t h e r a p y u s i n g 5 - F U , A DM ,a n d MMX r i o r t o t r e a t m e n t W i t h TPA. Blood r o ? l e a f t e rc h e m o t h e r a p y b u t b e f o r e TPA t r e a t m e n t ) : WBC: 1 . 9 > < 1 0 9 / L ; n e u t r o p h i l : 7 9 ; l y m p h o c y t e : 2 1 ; H b :1 1 6 g / L . Blood p r o ? l e ( t h r e e d a y s a f t e r TPAa d m i n i s t r a t i o n ) : WBC. . 9 > < 1 0 9 / L ; n e u t r o p h i l : 7 3 ; l y mp h o c y t e : 2 7 ; H b : 1 2 3 g / L . B l o o d p r o ? l e 7 d a y s a f t e rTPA d m i n i s t r a t i o n ) : WBC: 7 . 6 > < 1 0 9 / L ; n e u t r o p h i l : 8 2 ;l y m p h o c y t e : 1 8 ; H b : 1 1 8 g / L . Symptoms a f t e r T P A :C h i l l s , f e v e r 3 9 . 2 C . ) c o n t i n u e d f o r 4 h r s . L i v e r , k i d n e y ,h e a r t a n d l u n g W e r e f u n c t i o n i n g n o r m a l l y .

    S u b j e c t N o . 2 3 ) J . H . , m a l e , 5 5 . D i a g n o s i s : s t o m a c h c a r d i a )c a n c e r , r e o c c u r r e d a f t e r p r i o r s u r g e r y . The p a t i e n t h a dr e c e i v e d 5-FU and MMC. b e f o r e t r e a t m e n t W i t h TPA.B l o o d p r o ? l e b e f o r e TPA a d m i n i s t r a t i o n ) : WBC: 2 . 3 > < 1 0 9 / L ; n e u t r o p h i l : 5 3 ; l y m p h o c y t e : 4 7 ;H b : 1 2 3 g / L . B l o o d p r o ? l e f o u r d a y s a f t e r T P A ) : WBC:3 . 9 > < 1 0 9 / L ; n e u t r o p h i l : 4 4 ; l y m p h o c y t e : 5 6 ; H b : 1 2 9g / L . B l o o d p r o ? l e ( s e v e n d a y s a f t e r T P A ) : WBC: 3 . 7 >

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    TPA d m i n i s t r a t i o n ) : WBC: 1 . 1 > < 1 0 9 / L ; n e u t r o p h i l : 7 3 ;l y m p h o c y t e : 2 7 ; H b : 1 1 2 g / L . B l o o d p r o ? l e o n e d a ya f t e r a d m i n i s t r a t i o n o f 0 . 2 5 m g T P A ) : WBC: 5 . 3 > < 1 0 9 / L ;n e u t r o p h i l : 6 0 ; l y m p h o c y t e : 4 0 ; H b : 1 3 9 g / L . Symptoms a f t e r TPA: No h i l l s , no f e v e r , no l o c a l i r r i t a t i o n .L i v e r , k i d n e y , h e a r t a n d l u n g W e r e f u n c t i o n i n g n o r m a l l y.S u b j e c t N o . ( 2 5 ) TL, f e m a l e , 4 2 . D i a g n o s i s : b r e a s t c a n c e r .T h e p a t i e n t r e c e i v e d c h e m o t h e r a p y t r e a t m e n t u s i n g CTX,MMC, a n d 5 - F U . B l o o d p r o ? l e b e f o r e T P A ) : WBC:2 . 1 > < 1 0 9 / L ; n e u t r o p h i l : 7 2 ; l y m p h o c y t e : 2 8 ; H b : 1 2 6g / L . B l o o d p r o ? l e ( o n e d a y a f t e r a d m i n i s t r a t i o n o f 0 . 2 5m g f T P A ) : WBC: 6 . 4 > < 1 0 9 / L ; n e u t r o p h i l : 9 0 ; l y m p h oc y t e : 1 0 ; H b : 1 2 6 g / L . Symptoms a f t e r TPA a d m i n i st r a t i o n : No h i l l s , no f e v e r . I n j e c t i o n s i t e Was r e d , s W o l l e ni n a p p e a r a n c e a n d p a i n f u l p r o b a b l y c a u s e d b y t h e i n f u s i o nn e e d l e . T h e symptoms d i s a p p e a r e d t h e s e c o n d d a y a f t e rt h e y a p p e a r e d . L i v e r , k i d n e y , h e a r t a n d l u n g W e r e f u n ct i o n i n g n o r m a l l y .

    S u b j e c t N o . 2 6 ) Q . W . , m a l e , 5 6 . D i a g n o s i s : e s o p h a g e a lc a n c e r Which had m e t a s t a s i Z e d t o t h e l i v e r a f t e r s u r g e r y .T h e p a t i e n t h a d r e c e i v e d c h e m o t h e r a p y u s i n g c i s p l a t i na n d t a X o l . TPA d o s a g e : 0 . 2 5 m g . B l o o d p r o ? l e ( b e f o r eTPA d m i n i s t r a t i o n ) : WBC: . 6 > < 1 0 9 / L ; n e u t r o p h i l : 8 0 ;l y m p h o c y t e : 2 0 ; H b : 1 2 4 g / L . B l o o d p r o ? l e o n e d a ya f t e r TPA d m i n i s t r a t i o n ) : WBC: 4 . 2 > < 1 0 9 / L ; n e u t r o p h i l :8 3 ; l y m p h o c y t e : 1 7 ; H b : 1 2 0 g / L . B l o o d p r o ? l e 2d a y s a f t e r T P A ) : WBC: 5 . 6 > < 1 0 9 / L ; n e u t r o p h i l : 8 1 ;l y m p h o c y t e : 1 9 ; H b : 1 1 6 g / L . Symptoms a f t e r TPAa d m i n i s t r a t i o n : t e m p e r a t u r e r e a c h e d 3 9 C . Which l a s t e d3 h r . Stomach a c h e a n d d i a r r h e a ( W h i c h d i s a p p e a r e d s o o na f t e r ) . T h e c a r d i a c , h e p a t i c , r e n a l a n d p u l m o n a r y f u n ct i o n s Were normal.A b b r e v i a t i o n sV P 1 6 , E t o p o s i d e ; MMC, M i t o m y c i n C ; MTX, M e t h o t re X a t e ; 5 F U , 5 - ? u o r o u r a c i l ; C T X , C y c l o p h o s p h a m i d e ; C P ,C i s p l a t i n ; V D 3 , v i t a m i n D 3 ; MDS-RAEB, M y e l o d y s p l a s t i c

    s y n d r o m e - r e f r a c t o r y a n e m i a W i t h e X c e s s o r b l a s s t ; A r a - C ,c y t a r a b i n e ; AML, A c u t e m y e l o c y t i c l e u k e m i a ; M l , AMLW i t h o u t d i f f e r e n t i a t i o n ; M2, AML i t h m a t u r a t i o n ; M3,A c u t e p r o m y e l o c y t i c l e u k e m i a ; M 4 , A c u t e m y e l o m o n o c y t i cl e u k e m i a ; M 5 , A c u t e m o n o c y t i c l e u k e m i a ; R T , R e t e n t i o nt i m e ; WBC, W h i t e b l o o d c e l l s ; H b , H e m o g l o b i n .

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