SABrSemi-Autonomous Bioreactor

Group 10:

Jenna Ajello, Hayley Fritz, Tyler Le, Ryan Oliver,

Brooke Towns, Loc Tran, Alec Weaver

Outline1. Hedgehog Concept and Product Overview 2. Sub-System Breakdown3. Competitive Advantages4. Cost and Conclusions

Hedgehog Concept

❏ SABr optimizes the user experience by offering the most autonomous machine possible❏ Automated liquid handling between commercially available

well plates or conical tubes to custom culture plate❏ Self-sanitizing design reduces customer interference

Product Overview

❏ Stainless steel dispensing needle and micropump

❏ Customizable well plate ❏ Three unique shaking

patterns❏ OD and FI data of

individual wells

❏ Size: 48 in long X 24 in wide X 24 in tall

❏ Weight: 175 lbs❏ Power Consumption: 660

W maximum

Product Overview

Microbio Subsystem

Dynamics and Translation Subsystem

Shaking Plates

Sensing and Imaging Subsystem

Heating and Cooling Subsystem

Liquid Handling Subsystem

Structure Subsystem

Liquid Handling Capabilities

❏ Dispensing mechanism to release desired fluid into well plates

❏ Capable of disposing and neutralizing waste and disinfecting dispensing needle

❏ The TRL for this design is level 3 because system has been demonstrated analytically, but not yet demonstrated in a laboratory environment

❏ OTS parts for this design have a TRL of 9

Liquid Handling Callout

Liquid Handling Customer NeedsRequirements Metrics Source Status

2. If powered, run from 120V AC electricity from a standard wall outlet with a 15-amp breaker.

Wattage from micropump, Pump will draw 0.3W

UF Biofoundary

System can operate with provided power source

10. Has a minimal operational lifetime of 10 years.

Off the Shelf pump lifetime of 10 years and reusable stainless steel dispensing needle

UF Biofoundary

System meets minimal operational lifetime

11. Production devices cannot exceed $10,000

This subsystem costs $1,137.66 UF Biofoundary

This cost included purchased parts, manufacturing time, and assembly time. It is about 11.4% of the $10,000 budge

33. Achieve a dispense rate of 300 uL per second

Maximum flow rate of 670 uL UF Biofoundary

Maximum flow rate achieved due to diameter of dispensing needle

34. Deposit the minumum and maximum volume desired by customer

Yes, can deposit minimum and maximum volume of fluid in the range of 5–20,000 µL

UF Biofoundary

Subsystem meets volume requirements desired by customer, due to volume of system, dispensing needle size, and pump precision

35. System can dispose and neutralize waste

UV light used to neutralize waste and then system disposes into waste container

UF Biofoundary

Can properly dispose and neutralize waste

Microbiological Capabilities ❏ Customized 384 acrylic well plate

❏ Five layer sandwich plate lid

❏ Five flow adjustment valves

❏ Flow meter to control gas flow

❏ The TRL for the microbiology subsystem is a level 2 due to its concept only being formulated and no prototype being completed.

Microbiological Callout

Microbiological Customer Needs Requirements Metrics Source Status

2. If powered, run from 120V AC electricity from a standard wall outlet with a 15-amp breaker.

1 Watt maximum from the flowmeter

UF Biofoundry Power can run from the 120V AC electricity from a standard outlet

10. Has a minimal operational lifetime of 10 years.

The flowmeter has a lifetime of 10 years

UF Biofoundry The flowmeter meets the requirement of being a minimum of 10 years.

11. Production devices cannot exceed $10,000.

$1,584.71 UF Biofoundry The cost of this subsystem takes up around 15% of the budget

18. Capable of incubation/hibernation periods from one hour to two weeks

12 Hours of battery lifetime UF Biofoundry Since the system can be kept on if the electricity goes out for 12 hours it should be enough time to get the electricity back on.

21. Can culture microbes in fully enclosed compartments or vessels that are easily interchangeable and that allow for media exchange.

SABr has one closed loop system that is enclosed with a sandwich lid and interchangeable with different containers.

UF Biofoundry This closed loop system can culture for fully enclosed vessels and allows for media exchange.

22. Accommodate existing culture plates of the following sizes: 6, 24, 48, 96, deep 96, 384.

With the customized well plate, it can hold the maximum size, 384.

UF Biofoundry Since the maximum size well plate is used for the customized well plates all of the other sizes can fit inside the system.

23. Accommodate existing conical tubes of the following sizes: 15mL & 50mL.

The translation system includes a section for conical tubes of both sizes.

UF Biofoundry The system does accommodate for the two different sizes of conical tubes.

Heating/Cooling Capabilities

❏ Evenly Distributed Convection Heating and Cooling

❏ Easily Removable from the Structure❏ Closed Loop Control❏ symmetric design increases ease of

manufacturing❏ TRL 3 design has been proven

analytically, but a prototype has not been made. The component are at a TRL 9.

Heating/Cooling Component Callout

Heating/Cooling Customer NeedsDESIGN REQUIREMENTS METRIC SOURCE STATUS

2. If powered, run from 120V

AC electricity from a standard

wall outlet with a 15-amp

breaker.

The subsystem uses a maximum of 487.8 W

to maintain a constant temperature which is

27% of the total power

UF

Biofoundry487.8 W (27%)

1. Production device cannot

exceed $10,000.The subsystem cost $1,839.06

UF

Biofoundry$1,839.06

19 Can perform culture

condition closed loop control.

The system includes a temperature sensor

with closed loop control to control the

power into the peltier coolers.

UF

Biofoundry

DESIGN MEETS

REQUIREMENT

24. Uniformly heats wells/tubes

to prevent condensation.

Using a convection heating and cooling

reduces the temperature gradient between

the air and well plates.

UF

Biofoundry

DESIGN MEETS

REQUIREMENT

Sensing Capabilities

❏ OD and FI measurements through using mCherry filter cube set, a photomultiplier tube, and a separate phototransistor

❏ Technology Readiness Level 3❏ Concept validated experimentally❏ OTS parts evaluated individually

meet Level 9

Sensing Components Callout

Sensing Customer RequirementsREQUIREMENT METRIC SOURCE STATUS

2. If powered, run from 120V AC electricity from a standard wall outlet with a 15-amp breaker.

40 Watts maximum UF Biofoundry System can successfully operate with the given power source

10. Has a minimal operational lifetime of 10 years. 11 years UF Biofoundry System lifetime exceeds requirement

11. Production devices cannot exceed $10,000. $2,295.77 UF Biofoundry System takes almost 25% of budget

13. Programmable: control parameters can be changed, or more complex control can be added

Use of software like BioTek to aid dynamics system

UF Biofoundry Variable options to exceed customer requirement

14. Have an intuitive user interface Laptop connected to system omits handling of microscope slides

UF Biofoundry Autonomous experience using simple customer interference

28. Can measure OD and FI in all individual wells of any accommodated well plate readings to provide culture condition data

2 separate sensors for separate readings

UF Biofoundry Successfully measures both OD and FI

29. Designed with respect to trade-offs in using monochromators vs. filter cubes for FI readings

mCherry filter cube set UF Biofoundry Allows lower cost and higher sensitivity

30. Achieve a light intensity of 1 kW/cm2 at 640 nm for several minutes. High frequency wavelengths would operate at a lower intensity as it becomes lethal to cells.

600 W/cm^2 at 643 nm UF Biofoundry Lowered intensity at higher frequency allows for a factor of safety when reading live cells

Dynamics and Translation System❏ 3-axis motion

capabilities

❏ Up to three unique shaking patterns

❏ Closed loop control with integrated limit switches for fail-safe system

❏ Technology Readiness Level 2

❏ OTS parts evaluated individually meet Level 9

Dynamics and Translation System

Y-Axis Assembly

X-Axis Assembly

Dynamics and Translation System

Dynamics and Translation System Requirements

Overall Structure Capabilities

❏ The full-seamed weldment is made of 5052 aluminum with 2 access openings which are sealed to prevent contamination from external impurities

❏ The TRL is 2 for the overall structure because the concept has not yet been prototyped.❏ Changes are expected to occur once a physical product is made for testing

because the interaction between the customer and the product can’t be 100% predicted via models and formulas

24”

24”48”

3/32 O-ring per Parker spec for face seal

Parker 2-265 O-ring gland seal

Quick-Access port: utilized for insertion of cell cultures as well as quick cleaning operations

Hinged lid: utilized for assembly of internal components, maintenance, and deep sanitization operations

Lid

Structure weldment

Overall Structure - Component Callout

Quick access flange

Data acquisition laptop

LED indicators and E-stop

Lid latch

Lid hinge 2x

Overall Structure - Requirements Table

Custom Culture Plate

Design Highlights and Key Features

Multiple Access Points

Cost Analysis

OTS PART COST$6,607.31

MATERIAL AND MANUFACTURING COST

$4,611.96

TOTAL PROTOTYPE COST

$11,552.34

ASSEMBLY COST$318.03

Why Choose SABr?

Conclusions

The SABr is the ultimate solution to customer needs,

providing a mostly autonomous experience that is not

currently available for the price point.

We would like to thank our corporate sponsors Northrop Grumman and Cummins, faculty and staff for attending our presentation and supporting this project.

Thank you