building and imaging dna nanostructures from computational design jamarius waller university of...
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Building and Imaging DNA Nanostructures From
Computational Design
Jamarius Waller
University of Mississippi (Biochemistry)
Mentor: Dr. Randy Wadkins
Introduction
• DNA has been studied by its basic functions.• Scientist recently discovered novel uses for DNA.
– New methods involve folding, bending, and twisting DNA to make secondary structures.
• Exploration of uses of these new functions
Background: Cadnano/CanDo
• Computer software used to design DNA that was developed by biochemist at MIT
• Templates are formed in cadnano• Shaped DNA structure are rasterized from template in
CanDo.
Background: DNA Origami
– Common name for DNA nanostructures– The term DNA origami was developed around 20
years ago.– A few structures have been made using this
technique.
Background: AFM/ Gel E.
• (Atomic force microscopy)– Used to visualize DNA origami structures
• Gel Electrophoresis– Comparative band lengths and weights to determine
differences in DNA samples
Phases of Research
• 3 Phases – Computational Phase– Experimental Phase– Imaging Phase
Computational Phase
• Computer software CadNano/Cando• 3 step template process
Step 1
Step 2
Step 3
Results: Computational
Experimental Phase
• Combination of DNA strands– 1:100 Ratio of scaffold to staple
• Annealing of complimentary bases– Rapid heating to 95oC and a slow cooling to 20oC– Using a ramp rate of -1oC
Imaging Phase
• 2 Ways of Imaging– Gel electrophoresis– AFM
Gel 1: 4oC at 130V
LadderScaffoldFinal Product
AFM Images
• Under Construction
Discussion
• Why are we studying this?– Future Implications– Bio Carriers– Nanobots– Novel Techniques
Acknowledgements
Dr. Randy Wadkins PhD.
Dr. Donald Cole PhD.
Ms. Demetria Hereford
2013 McNair Scholars
GRE Class Instructors
QUESTIONS/COMMENTS?
Building and Imaging DNA Nanostructures From
Computational Design
Jamarius Waller
University of Mississippi (Biochemistry)
Mentor: Dr. Randy Wadkins