Cellometer

® K2

978-327-5340 | info@nexcelom.com | www.nexcelom.com

Scientists in Single Cell Genomics Choose Cellometer K2• Low sample volume: 2-20µl per sample• Accurate cell and nuclei counts of cell lines and

primary samples• Automated assessment of cell clumps• Broad dynamic range for concentration and

viability measurement• 30 seconds per count

User Proven Performance

Accuracy From Cell Lines to Primary Samples — Including Clumpy CellsK2 can be customized to handle a variety of cell types (primary cells, tumor digest, insect cells, cell lines, fragile cells, clumpy cells and more) at low or high concentrations.

Speed — 30 Seconds Per CountCellometer’s user-friendly workflow delivers cell count and viability in less than 30 seconds per sample, requires no incubation time and eliminates user-to-user variability.

CORD BLOOD MOUSE SPLENOCYTES

PBMC HEPATOCYTES

MELANOCYTES

Low Sample Volume, Complete

CountsCell samples in single cell genomics are precious. K2 requires only 2 µl to 20 µl

for accurate counts.

2 µl 20 µl

SF9 CELLS

The Automated Cell Counter for Single Cell Sequencing

SD100 Simply CountedCell Counting Chambers

SD025 Simply CountedCell Counting Chambers

ChromiumTM

10x Genomics

ddSEQTM

Bio-Rad

C1TM

Fluidigm

ChromiumTM is a product of 10x Genomics | ddSEQTM is a product of Bio-Rad | C1TM is a product of Fluidigm

“The Nexcelom K2 cell counter helped us significantly with reducing processing time, saving time for cell and nuclei counting, and also more accurate cell counts.”-Dr Christine Cheng, Boston University

Dr. Eliver Ghosn (ChromiumTM/K2 user)Emory University, Assistant Professor, Vaccine Center

Dr. Karen Abramson (ChromiumTM/K2 user)Duke University, Manager, Molecular Genomics Core

Pipette sample

Insert slide & count

Get images & data

Instantaneous labeling

Stain cells

Nexcelom products are for RESEARCH USE ONLY and are not approved for diagnostic or therapeutic use. © Copyright 2018 Nexcelom Bioscience LLC. All Rights Reserved. xxxxxxxx Rev x

Avoid Counting Debris and Residual RBCs in Fresh Human PBMC Samples. The captured bright field image shows RBCs (indicated by red arrows) that can lead to inaccurate counts during manual counting of PBMC samples. The Cellometer fluorescent acridine orange and propidium iodide (AO/PI) image on the left shows that RBCs were not counted.

Consumables That Work For YouWith two different disposable counting slides to choose from, complete counts are accurate using only 2 µl to 20 µl of sample. Image-based counting with disposable counting chambers offers several key advantages:

• No clogging• Ideal for fragile samples, such as hepatocytes• No washing• No sample cross-contamination• Fluorescent dye improves accuracy and

consistency of counts

SD100 slides are standard slide thickness, for cells ranging from 2 to 80 µm in diameter and SD025 slides are for cells < 20 microns in diameter.

† Using acridine orange / propidium iodide (AO/PI)‡ Using Calcein AM / propidium iodide (CAM/PI)+ Using propidium iodide (PI)* Using SD25 Cellometer Counting Chambers

Bright Field PBMC PBMC with AO/PI

Dual Fluorescent Staining for Clumpy, Fragile Hepatocyes. The fluorescent image (far right) shows bright green acridine orange (AO) positive hepatocytes declustered by the Cellometer algorithm. Red circled hepatocytes are PI positive (dead) while free nuclei are not counted. Bright Field AO/PI

Live AO positive

Free nuclei are not countedDead, PI positiveDeclustered clumpy cells accurately counted

Custom K2 Applications

Viability for Immune Cells†

Viability for “Fragile Cells”†

RBC+ Platelet Contamination Assay†

Viability of Low Concentration Samples†

QA/QC Pre Viability Using “Do Not Cluster” Viability for Low Volume Samples†*

Trypan Blue Count for SF9 CellsViability for Pigmented Cells‡

Total Nuclei Count+

REQUEST A FREE DEMO!

nexcelom.com/demo