cells

37
CELLS Molecular Biology Techniques

Upload: arty

Post on 23-Feb-2016

89 views

Category:

Documents


0 download

DESCRIPTION

Cells. Molecular Biology Techniques. Which type of cell has a nucleus?. Prokaryotic Eukaryotic. Eukaryotic versus Prokaryotic Cells. Prokaryotic - Cells lacking a nucleus. Eukrayotic - Cells containing a nucleus. Organelles - Membrane-bound bodies found within eukaryotic cells. - PowerPoint PPT Presentation

TRANSCRIPT

Page 1: Cells

CELLSMolecular Biology Techniques

Page 2: Cells

WHICH TYPE OF CELL HAS A NUCLEUS?

1 2

50%50%1. Prokaryotic2. Eukaryotic

Page 3: Cells

EUKARYOTIC VERSUS PROKARYOTIC CELLS Prokaryotic - Cells lacking a nucleus. Eukrayotic - Cells containing a nucleus.

Organelles - Membrane-bound bodies found within eukaryotic cells.

Page 4: Cells
Page 5: Cells

CELL STRUCTURE Cell wall:

Rigid boundaries of the cellCellulose: organic compound that makes up

the majority of plant cell walls Cotton is 90% cellulose

Hemicellulose: Gluelike substance that holds cellulose fibers together within the wall

Pectin: Organic matter that provides stiffness

Page 6: Cells

CELL STRUCTURE Cell (Plasma) Membrane: Binds all the living components within the protoplasm

Cytoplasm: All cellular components between the plasma membrane and the nucleus

Page 7: Cells

WHAT IS THE NAME OF THE JELLY LIKE SUBSTANCE WHERE MANY CHEMICAL REACTIONS TAKE PLACE AND CELL ORGANELLES ARE EMBEDDED?

1 2 3 4

25% 25%25%25%

1. Cytoplasm2. Chloroplast3. Nucleus4. Cellulose

Page 8: Cells

NUCLEUS Nucleus: Control center of the cell, houses DNA, DNA

provides information needed to fulfill the cells’ needs (growth, differentiation, etc) & stores heredity information Nuclear Envelope: Structurally complex pores,

proteins that act as channels for molecules are within the pores, acts as gatekeeper between nucleus and rest of cell

Chromatin: Composed of proteins and DNA, when nucleus divides for mitosis, chromatin coils.

Chromosomes: Condensed condition of chromatin. Each plant/animal cell has its own fixed number of chromosomes, radish has 18, humans have 46.

Page 9: Cells
Page 10: Cells

WHAT CONTROLS REPRODUCTION AND CONTAINS THE GENETIC INFO OF THE CELL?

1 2 3 4

25% 25%25%25%1. Cell wall2. Nucleus3. Nuclear

envelope4. Plastids

Page 11: Cells

ENDOPLASMIC RETICULUM (ER) Endoplasmic Reticulum facilitates cellular

communication and materials channeling. Connected to outer membrane of nucleus Rough ER distributes ribosomes Smooth ER associated with lipid secretion

Page 12: Cells
Page 13: Cells

RIBOSOMES Ribosomes are composed of two subunits

composed of RNA and proteins. involved in linking amino acids for the

construction of large protein molecules

Page 14: Cells

GOLGI APPARATUS are often bound

by branching tubules that originate from the ER. Involved in the

modification of carbohydrates attached to proteins synthesized and packaged in the ER.

Page 15: Cells
Page 16: Cells

PLASTIDS Plastids: associated with the storage or manufacturing

of carbohydratesChloroplasts: Location of photosynthesis, contain

stacks of grana Grana/Granum: contain thylakoids Thylakoids: contain chlorophyll Chlorophyll: give green pigment

Chromoplasts Found in colored plant organs, like fruits & petals. Some chloroplasts evolve into chromoplasts (like ripening

tomatoes). Leucoplasts

No pigment, assumed to be in roots. May become specialized for bulk storage of starch, lipid or

protein.

Page 17: Cells
Page 18: Cells

WHAT IS THE LOCATION OF PHOTOSYNTHESIS IN THE CELL?

1 2 3 4

25% 25%25%25%1. Plastids2. Chloroplast3. Chromoplasts4. Leucoplasts

Page 19: Cells

WHAT GIVES PLANTS THEIR GREEN PIGMENT?

1 2 3 4

25% 25%25%25%1. Grana2. Thylakoids3. Dictyosomes4. Chlorophyll

Page 20: Cells

MITOCHONDRIA Mitochondria release energy produced from

cellular respiration. Powerhouse of the cell

Page 21: Cells
Page 22: Cells

H2O

Glycolysis

Krebs Cycle

Electron Transport Chain

Page 23: Cells

VACUOLES Used for storage in

plants In mature cells, 90% of

volume may be taken up by central vacuoles bounded by vacuolar membranes.

Filled with cell sap which helps maintain pressure within the cell.

Also frequently contains water-soluble pigments, like anthocynanin which provide color for flowers.

Page 24: Cells

CYTOSKELETON Cytoskeleton is an

intricate network of microtubules and microfilaments. Microtubules control

the addition of cellulose to the cell wall.

Page 25: Cells
Page 26: Cells

SUMMARY Cytoplasm Jelly like substance where chemical reactions

take place and other parts are embedded. Nucleus Controls reproduction and also contains the

characteristics of the cell. Cell membrane Surrounds the cell and controls what

moves in and out of the cell. Mitochondria Found only in plant cells where respiration

occurs. Chloroplasts These makes plants green. They contain

chlorophyll which is needed for photosynthesis.

Page 27: Cells

WHAT GIVES CELLS THEIR SHAPE?

1 2 3 4

25% 25%25%25%1. Cell wall2. Cell membrane3. Sap4. Vacuoles

Page 28: Cells

SUMMARY Cell wall Gives the cell its shape. The cell's "skeleton". Vacuole Where the cell sap is stored, controls pressure Golgi Apparatus Involved in the modification of

carbohydrates attached to proteins synthesized Endoplasmic Reticulum Facilitates cellular

communication and materials channeling, distributes ribosomes

Ribosomes Involved in protein synthesis

Page 29: Cells

CELLULAR REPRODUCTION Cell division process referred to as cell cycle.

Divided into interphase and mitosis. Interphase

Period when cells are not dividing. G1 - Cell increases in size. S - DNA replication takes place. G2- Mitochondria divide, and microtubules produced.

http://www.youtube.com/watch?v=O3_PNiLWBjY

Page 30: Cells
Page 31: Cells

MITOSIS Mitosis refers to the process of cellular

division that produces two daughter cells with equal amounts of DNA and other substances duplicated during interphase. Each daughter cell is an exact copy of the parent

cell. Mitosis occurs in meristems.

Page 32: Cells

PROPHASE Chromosomes condense.

Strands of chromatin coil and tighten with centromeres holding each pair of chromatids together.

Nuclear envelope fragments.

Page 33: Cells
Page 34: Cells

METAPHASE Chromosomes align at the cell’s equator.

Spindle fibers collectively referred to as the spindle.

At the end of metaphase, the centromeres holding each sister chromosomes separate lengthwise.

Page 35: Cells

ANAPHASE Sister chromatids separate and are pulled to

opposite poles. Spindle fibers gradually shorten as material is

continuously removed from the polar ends.

Page 36: Cells

TELOPHASE Each group of daughter chromosomes become

surrounded by a nuclear envelope. Daughter chromosomes become indistinguishable. Nucleoli reappear Spindle fibers disintegrate Cell plate forms.