certificate of analysis€¦ · report date: 10-feb-17 13:06 (mt) version: certificate of analysis...

[This report shall not be reproduced except in full without the written authority of the Laboratory.]

03-FEB-17

Lab Work Order #: L1886901

Date Received:Cypher Environmental Ltd.

1149 St. Matthews Avenue2nd FloorWinnipeg Manitoba R3G 0J8

ATTN: Teaghan WellmanFINAL 10-FEB-17 13:06 (MT)Report Date:

Version:

Certificate of Analysis

ALS CANADA LTD Part of the ALS Group A Campbell Brothers Limited Company

____________________________________________

Judy DalmaijerAccount Manager

ADDRESS: 1329 Niakwa Road East, Unit 12, Winnipeg, MB R2J 3T4 Canada | Phone: +1 204 255 9720 | Fax: +1 204 255 9721

Client Phone: 204-489-1214

Job Reference: NOT SUBMITTEDProject P.O. #:

C of C Numbers:Legal Site Desc:

ALS ENVIRONMENTAL ANALYTICAL REPORT

L1886901 CONTD....2PAGE

Result D.L. Units Extracted AnalyzedSample Details/Parameters

of

Qualifier* Batch

* Refer to Referenced Information for Qualifiers (if any) and Methodology.

Version: FINAL 3

L1886901-1 40L OF 0.25% DUST STOPCLIENT on 03-FEB-17Sampled By:

LIQUID Miscellaneous Parameters

Trout Bioassay LC50 03-FEB-17See attached.

Matrix:

R3649799

TROUT-LC50-WP

Reference Information

Trout Bioassay LC50

L1886901 CONTD....

3PAGE of

Certified, disease-free rainbow trout (Oncorhynchus mykiss) are exposed to several concentrations of a sample including full strength, under static conditions in order to estimate the median lethal concentration (LC50) - the concentration of the sample in water that is estimated to be lethal to 50% of the test organisms within a 96-hour exposure period.

Samples with excessive salinity (reported as conductivity greater than 13700 µmhos/cm) discharging into marine waters will require alternate testing.

ALS Test Code Test Description

Water EPS 1/RM/13, EPS 1/RM/9

Method Reference**

** ALS test methods may incorporate modifications from specified reference methods to improve performance.

Matrix

The last two letters of the above test code(s) indicate the laboratory that performed analytical analysis for that test. Refer to the list below:

Laboratory Definition Code Laboratory Location

WP ALS ENVIRONMENTAL - WINNIPEG, MANITOBA, CANADA

Test Method References:

Chain of Custody Numbers:

GLOSSARY OF REPORT TERMSSurrogates are compounds that are similar in behaviour to target analyte(s), but that do not normally occur in environmental samples. For applicable tests, surrogates are added to samples prior to analysis as a check on recovery. In reports that display the D.L. column, laboratory objectives for surrogates are listed there.mg/kg - milligrams per kilogram based on dry weight of samplemg/kg wwt - milligrams per kilogram based on wet weight of samplemg/kg lwt - milligrams per kilogram based on lipid-adjusted weight mg/L - unit of concentration based on volume, parts per million.< - Less than.D.L. - The reporting limit.N/A - Result not available. Refer to qualifier code and definition for explanation.

Test results reported relate only to the samples as received by the laboratory.UNLESS OTHERWISE STATED, ALL SAMPLES WERE RECEIVED IN ACCEPTABLE CONDITION.Analytical results in unsigned test reports with the DRAFT watermark are subject to change, pending final QC review.

Version: FINAL 3

L1886901-1

12 Dec 2016 DJG

Page 1 of 5

Sample ID:

96-hour LC50 v/v (%):

95% Lower Confidence Interval (%):

95% Upper Confidence Interval (%):

Method of Calculation:

Confirmed by Graph:

Sample Origin:

Sample Description:

Sampling Date and Time:

Sampling Method:

Sampled By:

Container(s) Description:

Sample Volume:

Date and Time Received:

Transit Irregularities:

Storage Temperature (°C):

Test Organism:

Test Description:

Performed By:

Starting Date and Time:

Deviations from Reference Method:

Cypher Environmental

40L of 0.25% Dust Stop

03-Feb-17

2 x 20L polyethylene pails without liners

40L

EPS 1/RM/9, May 1996 with May 2007 amendments, Environment Canada

03-Feb-17 14:40

None

n/a

n/a

Not Provided

n/a

Test Information

None

n/a

Oncorhynchus mykiss

AGJ

03-Feb-17 16:45

Reference Method(s):

Rainbow Trout Bioassay Test Report - LC50

Sample Information

Summary Results

Non-Lethal

n/a

Acute, 96-hour, Static, LC50

EPS 1/RM/13, 2nd Ed. Dec. 2000, with 2007 and 2016 amendments, Environment Canada

L1886901-1

Grab

L1886901-1

12 Dec 2016 DJG

Page 2 of 5

Colour:

Odour:

Turbidity:

Solids:

Hardness (mg/L): 2.5 10 250

Alkalinity (mg/L): 1.4 10 140

Temperature (°C):

Dissolved Oxygen (mg/L):

Conductivity (µS/cm):

pH (5.5-8.5 pH units):

pH Adjustment:

pH Adjustment Procedure:

Aeration Time (min):

Sample Test Concentration (v/v):

Aeration Rate (5.5-7.5 mL/min/L):

Dissolved Oxygen (D.O.) Before Pre-Aeration (%):

Average D.O. After Pre-Aeration (%):

Lot Number:

Weekly Mortality Preceeding Test (%):

Sample Size:

Source of Holding/Dilution Water:

Container Description:

Aeration Method:

Aeration Rate (5.5-7.5 mL/min/L):

Test Solution Volume (L):

Test Solution Depth (cm):

Number of Test Organisms per Container:

Loading Density (g/L):

83.2 n/a n/a n/a n/a 96.3

6.1 ± 0.2 6.1 ± 0.2 6.1 ± 0.2 6.1 ± 0.2

75.7 n/a n/a n/a n/a 93.4

30

0.250% 0.125% 0.063% 0.031% 0.016% 0%

6.1 ± 0.2 6.1 ± 0.2

Test Organism Data

Conditions Common to All Concentrations During Test

Observations

Thermometer S/N 91154465

mL of Sample x 1000 =

mL of Sample x 1000 =

mL Titration Solution/

mL Titration Solution/

Mild

High

Initial Parameters

Dark Brown

Not Adjusted

8.86

6.15

696

High

n/a

S/N D01908

VWR Portable Conductivity Meter

VWR SympHony pH Meter

14.4

Pre-Aeration

11/01/17 T3

0

10

0.23

Dechlorinated UV Treated City of Winnipeg Tap Water

20 L Polyethylene Pail with Liner

Compressed air bubbled through silica-glass air diffuser

(as set during pre-aeration above)

20

34

10

S/N 51071543

YSI Dissolved Oxygen Meter S/N 15M102668

L1886901-1

12 Dec 2016 DJG

Page 3 of 5

0h 24h 48h 72h 96h 0h 24h 48h 72h 96h 0h 24h 48h 72h 96h

0 14 n/a n/a n/a 14 9.81 n/a n/a n/a 9.72 7.51 n/a n/a n/a 7.68

0.016 14 n/a n/a n/a 14 9.93 n/a n/a n/a 9.66 7.58 n/a n/a n/a 7.38





24h 48h 72h 96h 24h 48h 72h 96h

n/a n/a n/a 0 n/a n/a n/a 0






Mean Fork Length (mm):

Lower Range Fork Length (mm):

Upper Range Fork Length (mm):

Mean Wet Weight (g):

Number of Fish Stressed

38

36

40

Conditions During Test

0.016

Concentration

(% v/v)

Temperature (°C)

(15 ± 1°C)Dissolved Oxygen (mg/L) pH (pH units)

Number of Fish DeadConc.

(% v/v)

Conductivity

(μS/cm)

355

0.063

0.125

452

394

0h

332

700

Control Fish Information at End of Test

0

0.25

0.031

0.47

546

L1886901-1

12 Dec 2016 DJG

Page 4 of 5

LC50:

LC50 Lower 95% Confidence Limit:

LC50 Upper 95% Confidence Limit:

Statistical Method:

Reference Toxicant:

Date Reference Toxicant Initiated:

Recent 96h Reference Toxicant Test LC50 (mg/L Zinc):

Lower 95% Confidence Limit (mg/L Zinc):

Upper 95% Confidence Limit (mg/L Zinc):

Historic Geometric Mean LC50 (mg/L Zinc):

Lower 95% Confidence Limit (mg/L Zinc):

Upper 95% Confidence Limit (mg/L Zinc):

Method of Calculation:

Confirmed by Graph:

Reference Toxicant Test Results

Zinc Sulfate

0

Mortality and Stressed Behaviour Information

Conc.

(% v/v)

Dead

0.33

Note: Non-lethal = 0 mortality

Median Lethal Concentration Results for Multi-Concentration Tests

Non-Lethal

n/a

26-Jan-17

0

0

0

0.46

0.64

0.27

1.51

0

0

0

0

Mean Number of Fish at

End of Test

Mean Rate of Fish at End

of Test (%)

0 0

Dead Stressed

0 0

00

0.25

0.063

0.125

0

Stephan LC50 Program, Probit

0.016

0.031

0

0.23

Yes

0 0

n/a

Stressed

0

0

n/a

0

0 0

0 0

L1886901-1

12 Dec 2016 DJG

Page 5 of 5

Observations/Comments

No toxicity observed.

No sublethal biological effects observed.

Sublethal Biological Effects

certificate of analysis€¦ · report date: 10-feb-17 13:06 (mt) version: certificate of analysis...

Documents