Inbreeding session

chaired by Thomas Nissen

Verband der Züchter des Holsteiner Pferdes e.V.

WBFSH Seminar in Vienna

19th Ocotober 2015

Inbreeding session

• Johan Sölkner: Inbreeding and inbreeding depression:

News from a genomic perspective

• Barbara Wallner: Genetic characterisation of stallion

lines using Y-chromosomal markers

• Lukas Roos: Inbreeding in the Holstein sport horse

breed

Johan Sölkner: Inbreeding and inbreeding

depression: News from a genomic perspective

1978 - 1983 Study of Agricultural Sciences (BOKU, Vienna)

1984 - 1988 Dissertation at University of Natural Resources and Life

Sciences, Degree Dr. rer.nat.tech.

1991 Habilitation in Animal Breeding and Population Genetics at

University of Natural Resources and Life Sciences (Assoc. Prof.)

2000 - present Full Professor of Animal Breeding and Population Genetics, Vienna

2006 Visiting professor at University of Sydney, Australia

Several research fellowships in Australia

Johan Sölkner: Inbreeding and inbreeding

depression: News from a genomic perspective

• Introduction of the concept of runs of homozygosity for calculation of inbreeding

levels from SNP chip data and comparison of this information with pedigree

inbreeding

• The improved calculation of inbreeding levels with genomic data allows better

understanding on the effects of inbreeding depression (reduced fitness)

g genes causing inbreeding can be found

• In practice, recessive disorders, which are part of inbreeding depression, can be

systematically avoided

g by genotyping breeding animals and not mating individuals that are both

carriers of genetic variants causing particular recessive disorders

Barbara Wallner: Genetic characterisation of

stallion lines using Y-chromosomal markers

1991 - 1998 Study of veterinary medicine, University of Veterinary Medicine

Vienna

1998 - 2001 Doctoral student of Prof. Dr. Gottfried Brem (Institut für Tierzucht

und Genetik, Veterinärmedizinische Universität Wien)

Title: Selektive Klonierung von y-chromosomalen DNA-Sequenzen

beim Pferd mittels „Representational Difference Analysis“

2000 - present Assistant at the Institute of Animal Breeding and Genetics,

University of Veterinary Medicine Vienna

Research fellowship at the University of Porto, Portugal

Barbara Wallner: Genetic characterisation of

stallion lines using Y-chromosomal markers

• The presentation shows that polymorphic markers on the Y chromosome are useful

to elucidate the origin of individual stallion lines and enable a pedigree-independent

genetic characterisation of stallion lines

• An analysis of the genetic material of the Y chromosome can give information on

the male ancestry (Y chromosomes only passed from fathers to sons)

• Analyzing of the Y chromosomes of stallions from different European and American

breeds using the new sequencing technologies

• Y chromosome data clearly show influence of stallions from the Middle East to

European and American breeds

Inbreeding session

chaired by Thomas Nissen

Verband der Züchter des Holsteiner Pferdes e.V.

WBFSH Seminar in Vienna

19th Ocotober 2015

Casall Cristallo Carlina

Carlo

Thomas Nissen

Line founder of the Holsteiner breed

in the 19th century

Adjutant v. Midas 1886 – 1913

Ethelbert v. St. Fargans xx 1874 – 1897

Achill v. Herkules 1880 - 1904

Dr. Axel de Chapeaurouge

1861 – 1941

Thomas Nissen

Originally physician fascinated by the

thoroughbred breeding

Doing research in:

• thoroughbred breeding

• comparing all warmblood breeds

• Recognition of the importance of the

stallion Achill

Scientist of genetics and performance

Gustav Fehrs

• student of Dr. Chapeaurouge

• Scientific work: „Das

Holsteiner Marschpferd“

Thomas Nissen

Achill

Dr. Thomas Nissen

Sons and stallions of the Achill line

Thomas Nissen

Achill

Achill - Meister

- Nerv

- Siegmund

- Tobias – Favorit – Fanal- Fanatiker

– Fachmann – Fähnrich

- Farnese

Farmer Fridericus Fleming

Thomas Nissen

Achill-Libero(27 times inbreeding of Achill)

Thomas Nissen

Landgraf Ladykiller xx Sailing Light xx Blue Peter xx

Solar Cygnet xx

Lone Beech Loaningdale xx

Fartuche xx

Warthburg Aldato Anblick xx

Kreta

Schneenelke Fangball

Blümchen

Oktave Ronald Ramiro Raimond

Valine

Adrette Heilbutt

Dorette

Gelonika Komet Korenbleem xx

Valet

Loni Löwenjäger

Emmia

27 x

Achill in

his

pedigree

Offspring from Achill

Dr. Thomas Nissen

Lambrasco Numero Uno

• 60 horses jumping 160 cm

• 12 approved stallions (KWPN, SF, CH, HOLST, HANN, OLD)

Lukas Roos: Inbreeding in the

Holstein sport horse breed

2004 - 2010 Study of Agricultural Science (Animal Science),

Humboldt University Berlin and Christian Albrechts University Kiel

2011 - 2014 Dissertation at the Institute for Animal Breeding and Husbandry,

Christian Albrechts University Kiel, Prof. Dr. J. Krieter

Title: Reproduction and health in Holstein Warmblood mares

- Impact of population structure and data recording -

2015 - present Timac Agro Deutschland, Regional Sales Manager

Lukas Roos: Inbreeding in the

Holstein sport horse breed

• A pedigree data set including 129.923 Holstein warmblood horses was analyzed

• Aim: Determination of genetic diversity, coefficients of inbreeding, the age of

inbreeding and the genetic contributions of founder animals and foreign

breeds

• Reference population includes all horses which had been born between 1990 and

2010

• A slight increase in the effective population size and a stagnation of inbreeding

during the last generation

g Impact of more open access given to foreign stallions in the recent past?