challenges in garlic breeding
DESCRIPTION
The limitations and future strategies for developing variability and seed husbandry for successful sexual reproduction in garlic.TRANSCRIPT
USMAN IJAZASSISTANT RESEARCH OFFICER
CHALLENGES IN CHALLENGES IN GARLIC BREEDINGGARLIC BREEDING
Allium sativum Family Alliaceae
Oldest crop 5000 years ago in Egyptian It was grown 4000 years
ago Used 2000 years ago in
China
Source: Agri. Statistics of Pakistan 2010-11
Source: Agri. Statistics of Pakistan 2010-11
Source: Agri. Statistics of Pakistan 2010-11
Source: Agri. Statistics of Pakistan 2010-11
Source: Agri. Statistics of Pakistan 2010-11
Province Area(Ha)
Production
(Kg)
Yield(Kg/Ha)
Punjab 2790 23018 8281Sindh 2790 9954 4990KPK 2090 22954 11000
Baluchistan
418 3318 8118
Pakistan 7281 59427 8181
Source: Agri. Statistics of Pakistan 2010-11
Central Asia Wild relative Allium
longicuspis (Etoh & Simon, 2002)
Wild progenitor Allium tuncelianum
(Mathew 1996) Currently found in Turkey
Diploid 2n = 2X = 16 Allium sub-species included
• Allium sativum var. ophioscorodon• Allium sativum var. pekinense• Allium sativum var. sativum
Non-Bolting Incomplete or partial bolting
Flowering ability Leaf traits Bulb traits Plant maturity Cold hardiness Bulbil traits Flower traits
Morphological variation is always complicated
Isozymes:Few polymorphism detected RAPDs:High polymorphism detected but un
reproducible AFLPsHighly conserved and reproducible Karyotype
• Basic karyotype for hardneck• Non Basic karyotype for
softneck
BULB: Consist of cloves surrounded by
dry basal sheath Bolting: 4-12 cloves of uniform
size Non-Bolting: 8-50 cloves of
variable sizeClove: Outer thin protective leaf + Inner
thick storage leaf Storage leaf subtends a
vegetative bud over flattened basal plate
Dormancy:Exposed to cool temperature (15 C or
below)Roots:Adventitious roots start developing basal
plateLeaf: Sprout leaf start elongation and ooze
out Leaves arise in opposite and alternate
orientationPseudostem: Base of consecutive leaves extensively
overlapped
Inflorescence: Umbel 150-200
flowers/umbel Protanderous Bulbils
Perfect / Hermaphrodite Petals: 6 Anthers: 6 Locules: 3 consisting of 2
ovules each Style longer than petals Garlic flower is smaller than
onion
Vernalization (<5 C) Floral induction Scape elongation Inflorescence
development Competition with bulb Floral maturation
Only bolting genotypes are sensitive
Accumulation of pathogens Chromosome aberrations Post-Meiotic tapetal
degeneration Unsuccessful competition with
bulbils Multivalent chromosome
pairing Long-term asexual propagation
Garlic germplasm Diverse Wild relatives Selection Central Asian garlic germplasm
ProcedureDiscovery of male fertile garlic sourcei. Pollination and seed productionSowing timeFlower color: Fertility, Hybrid seed productionBulbil removal: Early senescenceMultiple pollinationsPollinators: Honey bees, House flies, Bluebottle fliesDiseases & pestsWeather: Hot weather (>30 C)
ii. Seed harvest: Dried inflorescence crushed Small seeds are inviable Wind column Select heavier and vigorous
seed 50 seeds/umbel
iii. Growing seedlings: Difficult in early generations Seed treatments: Hormones,
Scarification, Stratification Epigeal germination
Selection for improved floral characteristics
Selection for male fertile flowers Selection for improved seed size
and vigor Selection for seedling vigor
Development of new clones for asexual propagation
Development of seed cultivated garlic cultivars
• Recurrent phenotypic selection• Hybrid garlic cultivars• Open pollinated field cultivars
Not realistic Sexual reproduction is the
necessity T. Etoh, confirmed that garlic seed
is possible USPTO lists three seed derived
garlic cultivars
Clonal selection Partially bolting types Improving production
technology Disease and pest tolerance Improving storage
technology Quality
Germplasm• VRIG-11• G11-1(N)• G11-2(H)• Lahsen Gulabi
VARIETIES Fresh bulb
size(mm)
No of cloves
Fresh bulb
weight
Yield(Tons/Hac)
VRIG-11 59 40 77 51Lehsan Gulabi
51 27 53 34Note: Average was taken for 20 bulbs
VARIETIES Leaf length (cm)
Leaf Width (cm)
No. of Leaves/pl
ant
Plant Height (cm)
VRIG-11 64.8 2.42 10.1 104.8Lehsan Gulabi
56.8 2.27 10.7 91.4
Chemical composition (%)
VRIG-11 Lehsan Gulabi
Moisture 41 34.5
Dry matter 59 65.5
Protein 6.65 6.86
Fat 0.48 0.59
Fiber 2.14 2.81
Mineral 2.43 2.74
Carbohydrates 48.31 52.54
Dry Powder 24.12 29.67
Energy (K Cal/100g)
212.08 229.85
Cultivar Days to maturity
Yield (tonnes/ha)
Avg. wt (g)/bulb
Avg.wt(g)/clove
Chinese 207 21.30 81.45 5.13 Lehson Ghulabi
190 9.49 39.10 1.73
GS-I 185 9.03 38.92 1.0Mehmood et al, 2000 (Sarhad J. Of Agri.)
Collection of diverse germplasm
Identification of flower producing pockets
Artificial floral induction Molecular markers Somaclonal variation
Asexual propagation, resulted clones do not flower, nearly or
completely sterile, first generation seedlings are very week. But, access to diverse germplasm, adopting new
procedures, careful seedling husbandry, allowed to produce
true seed garlic. Taking advantage of these
breakthrough, garlic breeding is underway
Dr. Shahid Niaz Director, VRI, Faisalabad Mr. Najeeb Ullah Botanist, VRI, Faisalabad Mr. Tahir Iqbal Asst. Botanist, VRI,
Faisalabad