chapter 6 - tour of the cell

3/9/13 Chapter 6 - Cells

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Chapter 6 - Cells

Due: 9:00am on Wednesday, February 27, 2013

Note: You will receive no credit for late submissions. To learn more, read your instructor's Grading Policy

Chapter 6 Question 2

Part A

The advantage of light microscopy over electron microscopy is that

ANSWER:

Correct

Activity: Prokaryotic Cell Structure and Function

Click here to complete this activity.

Then answer the questions.

Part A

_____ are surface appendages that allow a bacterium to stick to a surface.

ANSWER:

Correct

Pili enable bacterial cells to stick to a surface.

Part B

What is the function of a bacterium's capsule?

Biol 1002 - Spring 2013

Chapter 6 - Cells Resources

specimen preparation for light microcopy does not produce artifacts.

light microscopy provides higher contrast than electron microscopy.

light microscopy provides for higher resolving power than electron microscopy.

light microscopy provides for higher magnification than electron microscopy.

light microscopy allows one to view dynamic processes in living cells.

Pili

Ribosomes

Cell walls

Mitochondria

Flagella

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ANSWER:

Correct

A bacterium's capsule has a protective role.

Part C

The DNA-containing region of this bacterial cell is indicated by the letter _____.

ANSWER:

Correct

The nucleoid region contains DNA.

Part D

Where is a bacterial cell's DNA found?

ANSWER:

Correct

Bacteria lack a nucleus; their DNA is found in the nucleoid region.

Part E

In a bacterium, where are proteins synthesized?

ANSWER:

protection

DNA storage

protein synthesis

propulsion

adhesion

A

B

C

D

E

ribosomes

nucleus

peroxisome

nucleoid region

capsule



Correct

Ribosomes are involved in the manufacture of polypeptides (proteins).

Part F

What name is given to the rigid structure, found outside the plasma membrane, that surrounds and supports the bacterial cell?

ANSWER:

Correct

The cell wall is a rigid supporting structure.

Part G

The _____ is the bacterial structure that acts as a selective barrier, allowing nutrients to enter the cell and wastes to leave the cell.

ANSWER:

Correct

The plasma membrane is selectively permeable.

Part H

The structure that regulates the passage of material into and out of this bacterial cell is indicated by the letter _____.

ANSWER:

ribosomes

nucleus

peroxisome

nucleoid region

capsule

capsule

pili

cell wall

flagella

nucleoid region

plasma membrane

nucleoid region

ribosome

pili

cell wall



Correct

The plasma membrane is selectively permeable.

Activity: Role of the Nucleus and Ribosomes in Protein Synthesis



Part A

Beginning within the nucleus, the first step leading to the synthesis of a polypeptide is _____.

ANSWER:

Correct

Transcription is the first of the two main steps of protein synthesis.

Chapter 6 Pre-Test Question 8

Part A

Which of the following are common traits of chloroplasts and mitochondria?

Hint 1.

Mitochondria and chloroplasts each appear to have evolved from free-living bacterial forms.

ANSWER:

A

B

C

D

E

translation of a DNA nucleotide sequence into a sequence of amino acids

transferring of information from DNA to messenger RNA

linking of nucleotides to form a polypeptide

translation of an RNA nucleotide sequence into a sequence of amino acids

removal of introns from RNA and the stitching together of exons





Correct

Mitochondria and chloroplasts contain their own DNA and ribosomes.

Tour of a Plant Cell: Structures and Functions (BioFlix tutorial)

Plant cells have many of the samestructures found in animal cells, but plantcells also have unique structures that suittheir functions as sugar producers.

Before beginning this tutorial, watch theTour of a Plant Cell animation.

Part A - Comparing plant cells and animal cells

Plant cells and animal cells share many of the same structures, but each type of cell also has unique structures. In this activity, you will indicatewhich cell structures are found only in plant cells, only in animal cells, or in both plant and animal cells.

Drag each cell structure to the appropriate bin. If a structure is found in both plant cells and animal cells, drag it to the "both" bin.

Hint 1. Review of animal cell structures

To review animal cell structures, watch the Tour of an Animal Cell animation.

Hint 2. Which structures are unique to plant cells?

Plant cells have unique structures that function in photosynthesis. In addition, plant cells have other structures not found in animal cells thatfunction in support and storage.

Which cell structures are unique to plant cells? Select the three that apply.

ANSWER:

ANSWER:

Both reproduce by meiosis.

Both are surrounded by a single membrane.

Both have their own DNA.

Both are found in plant and animal cells.

Proteins for both are synthesized on ribosomes in the rough ER.

lysosome

central vacuole

cellulose cell wall

chloroplast

mitochondrion

endoplasmic reticulum



Correct

Plant cells and animal cells have more in common than they have differences. Nevertheless, plant cells’ unique structures play important roles.Photosynthesis occurs in plant cells’ chloroplasts. The plant cell’s central vacuole takes up most of the space within the cell and serves a varietyof functions, including storage and hydrolysis of organic compounds. Plant cell walls, which are composed primarily of cellulose, protect the cellsfrom damage and maintain their shape. Cellulose is the most abundant organic compound on the planet.

Part B - Plant organelle functions

Drag the correct description under each cell structure to identify the role it plays in the plant cell.

Hint 1. Structure and function of cell organelles

The structure of each organelle in a eukaryotic cell makes it very well-suited for the task it performs. Some examples are described here.



Golgi apparatus: The Golgi is composed of a series of flattened membranous discs called cisternae. Each cisterna forms anenclosed space that houses a distinct set of enzymes used to process proteins in a sequential manner as they are prepared fortransport to different sites.Mitochondria: The inner membrane of a mitochondrion has extensive infoldings, which greatly increase the surface area availablefor key reactions in the energy-producing processes of cellular respiration.

ANSWER:

Correct

Each organelle in a plant cell carries out a specific function. Mitochondria and the Golgi apparatus serve the same functions in both plant cellsand animal cells.

Part C - Structure and function of the chloroplast

The structure of a chloroplast is closely tied to its function as the site of photosynthesis.

Some of the following statements are true for chloroplasts; others are true for mitochondria.

Which statements are true for chloroplasts? Select the three that apply.

Hint 1. The role of chlorophyll

In order for chloroplasts to carry out photosynthesis, they need pigments that can absorb light energy from the sun. One important chloroplastpigment is chlorophyll, which absorbs light in the red and blue parts of the spectrum. Chlorophyll is the reason plants appear green.

Hint 2. How does chloroplast structure compare to mitochondrion structure?

Mitochondria and chloroplasts are similar in structure, but they also have key differences related to their respective functions in the cell.



Drag each label to the appropriate bin. If a structure is found in both chloroplasts and mitochondria, drag it to the "both" bin.

ANSWER:

ANSWER:

Correct

The chloroplast and the chemical reactions associated with it are extremely important for all living things. The photosynthetic reactions areresponsible for converting solar energy into chemical energy that is used not only by the cells in which photosynthesis occurs but also by otherorganisms. For example, all of the foods you eat can be traced back to a plant or other photosynthetic organism. Furthermore, the chloroplastand its reactions have had a profound influence on the constitution of Earth’s atmosphere due to the fact that oxygen is a byproduct ofphotosynthesis.

Connections Between Cells

They have membranous sacs called thylakoids that are surrounded by a fluid called stroma.

They are the sites of reactions that convert solar energy into chemical energy.

They are the sites of reactions that convert chemical energy to ATP.

They contain the green pigment chlorophyll.

Their matrix contains enzymes that function in cellular respiration.

Their inner membrane has infoldings called cristae.



Plants and animals are made up of billions of cells. These cells are surrounded by extracellular components that facilitate their interactions and provide asuitable environment for the construction and protection of tissues.

Part A - Comparing the extracellular environments of plants and animals

The cellular organization and extracellular environments of plants and animals are very different.

Drag the labels to their appropriate locations in the table below. Use only pink labels for the pink targets. Labels may be used more thanonce.

Hint 1. How to approach this problem

At the cellular level, the biggest difference between plant tissues and animal tissues is not what is inside the cells, but rather what is outsidethe cells. As you read each statement, consider how the presence or absence of a cell wall might influence intercellular interactions. Whichtypes of junctions do not exist in the presence of a cell wall?

Hint 2. How are animal cells connected to each other?

Animal cells have three main types of intercellular junctions. The occurrence of these junctions depends on the type of cells you are studying.For example, epithelial cells are rich in all three types of intercellular junctions.

Drag the labels onto the diagram to identify the different intercellular junctions found in animal tissues.

ANSWER:

Correct

The three main types of intercellular junctions in animal tissues are tight junctions, desmosomes, and gap junctions. Tight junctions anddesmosomes hold neighboring cells together, and gap junctions provide channels through which neighboring cells can communicate.

Hint 3. How do substances move between plant cells?

It is well known that cell walls in plants protect the cells from excessive uptake of water, but these walls also serve as a barrier to viruses. It hasbeen observed, however, that once a plant cell becomes infected, the viruses can easily move throughout the plant despite the fact that a wallsurrounds each cell.

Which structures enable a virus to infect an entire plant quickly?

ANSWER:

tight junctions

desmosomes

gap junctions

plasmodesmata



ANSWER:

Correct

A primary distinction between plant cells and animal cells is the fact that plant cells are surrounded by cell walls. If it were not forplasmodesmata, the cell walls would essentially imprison the plant’s cells, making intercellular communication and the distribution of nutrientsnearly impossible.Animal cells have a variety of intercellular junctions, including tight junctions, desmosomes, and gap junctions. The gap junctions of animal cellsare most similar to the plasmodesmata of plants. Gap junctions tend to be limited to tissues that require rapid and coordinated signal relaysystems, such as heart muscle; they play no role in nutrient distribution.

Activity: Membrane Structure



Part A

What is the function of Structure E?

ANSWER:



Correct

Cholesterol helps to stabilize the structure of the plasma membrane.

Part B

Identify Structure D.

ANSWER:

Correct

Phospholipids can be recognized by the presence of a head and two tails.

Part C

Identify Structure A.

ANSWER:

detection of environmental change

stabilization of the phospholipids

cell-cell communication

transport across the plasma membrane

structural support of the cell

protein

phospholipid bilayer of membrane

extracellular matrix

cholesterol

glycoprotein

protein

cholesterol

glycoprotein

extracellular matrix

phospholipid



Correct

Structure A is composed of both a carbohydrate and a protein.

Activity: Selective Permeability of Membranes



Part A

Structure A in this animation is a(n) _____.To view the animation, click here. Then click on structure A in the image to start the animation.

ANSWER:

Correct

The protein is allowing solute molecules to enter the cell.

Part B

Which of these cannot rapidly pass directly through the phospholipids of the plasma membrane?

ANSWER:

enzyme

receptor molecule

transport protein

structural protein

antibody

D only

C only

B, C, and D

B only

A only






Correct

Ions, such as hydrogen ions, and hydrophilic molecules, such as water and glucose, cannot rapidly pass directly through the phospholipids of aplasma membrane. To move rapidly through the membrane, they must pass through membrane transport proteins.

Osmosis

Osmosis is thenet movement of water across a semipermeable membrane from an area of lower solute concentration to an area of higher solute concentration. The waterwill continue to move across the semipermeable membrane as the system attempts to reach equilibrium, where both solutions have the sameconcentration.

There are three different ways a solution can be described in relation to a cell placed in the solution: isotonic, hypotonic, and hypertonic. Compared to acell, an isotonic solution exerts the same osmotic pressure as the cellular fluids. In other words, it behaves as if it had the same solute concentration asthe cell. A hypertonic solution behaves as if it had a higher solute concentration than the cell. A hypotonic solution behaves as if it had a lower soluteconcentration than the cell.

If a cell is placed in an isotonic solution, nothing happens to the cell because water moves into the cell at the same rate that it moves out ofthe cell.If a cell is placed in a hypotonic solution, water will flow into the cell, causing it to swell (and possibly burst).If a cell is placed in a hypertonic solution, water will flow out of the cell, causing it to shrink.

Part A - Determining water movement in osmosis

A semipermeable membrane is placed between the following solutions.

Which solution will decrease in volume?

Hint 1. Movement of molecules in osmosis

During osmosis, only the water molecules can move across the semipermeable membrane. The larger solute molecules cannot pass throughthe membrane. The water flows across the membrane in both directions; however, the net movement of water is into the solution with the highersolute concentration.

Hint 2. In which direction is the net flow of water in osmosis?

Toward which solution will the net flow of water be?

ANSWER:

ANSWER:

toward Solution A: 1.4% (m/v) starch

toward Solution B: 7.62% (m/v) starch

Solution A: 1.4% (m/v) starch

Solution B: 7.62% (m/v) starch



Correct

The water molecules actually move in both directions, but they move to a greater extent toward Solution B because it has a higher soluteconcentration than Solution A. The net movement of water molecules causes Solution A to decrease in volume and Solution B to increase involume.

Part B - Further exploration of the movement of water in osmosis

A semipermeable membrane is placed between the following solutions.

Which solution will increase in volume?

Hint 1. Movement of molecules in osmosis

During osmosis, only the water molecules can move across the semipermeable membrane. The larger solute molecules cannot pass throughthe membrane. The water flows across the membrane in both directions; however, the net movement of water is into the solution with the highersolute concentration.

Hint 2. In which direction is the net flow of water in osmosis?

Toward which solution will the net flow of water be?

ANSWER:

ANSWER:

Correct

The water molecules actually move in both directions, but they move to a greater extent toward Solution D because it has a higher soluteconcentration than Solution C. This net movement of water molecules causes Solution D to increase in volume and Solution C to decrease involume.

Part C - Three types of solutions

A red blood cell has been placed into three different solutions. One solution is isotonic to the cell, one solution is hypotonic to the cell, and onesolution is hypertonic to the cell. Determine which type of solution is in each beaker based on the cell's reaction.

Drag each item to the appropriate bin.

Hint 1. What are the definitions of hyper-, hypo-, and isotonic?

Define the following types of solution in relation to a red blood cell.

Drag each word to the appropriate blank.

ANSWER:

toward solution C: 9% (m/v) NaCl

toward solution D: 12.4% (m/v) NaCl

Solution C: 9% (m/v) NaCl

Solution D: 12.4% (m/v) NaCl



ANSWER:

Correct

For a cell in an isotonic solution, water flows into the cell to the same extent that it flows out of the cell. If a cell is in a hypotonic solution, waterflows into the cell, which causes it to swell and potentially burst. For a cell in a hypertonic solution, water flows out of the cell, which causes it toshrink.

Part D - Animal cells: crenation and hemolysis

An animal cell placed in a hypertonic solution will shrink in a process called crenation. An animal cell placed in a hypotonic solution will swell andpotentially burst in a process called hemolysis.

To prevent crenation or hemolysis, an animal cell must be placed in an isotonic solution such as 0.9% (m/v) NaCl or 5.0% (m/v) glucose. This does notmean that a cell has a 5.0% (m/v) glucose concentration; it just means that 5.0% (m/v) glucose will exert the same osmotic pressure as the solutioninside the cell, which contains several different solutes.

A red blood cell is placed into each of the following solutions. Indicate whether crenation, hemolysis, or neither will occur.



Solution A: 3.21% (m/v) NaClSolution B: 1.65% (m/v) glucoseSolution C: distilled H2OSolution D: 6.97% (m/v) glucoseSolution E: 5.0% (m/v) glucose and 0.9%(m/v) NaCl

Drag each solution to the appropriate bin.

Hint 1. How to approach the problem

You know that a 5.0% (m/v) glucose solution will exert the same osmotic pressure as a red blood cell. You also know that a 0.9% (m/v) NaClsolution will also exert the same osmotic pressure as a red blood cell.This means that any solution with a higher total solute concentration than either of these isotonic solutions will be hypertonic to the cell.Conversely, any solution with a lower total solute concentration than an isotonic solution will be hypotonic to the cell.

Hint 2. How does crenation differ from hemolysis?

If a red blood cell is placed in a hypertonic solution, _____ will occur. If a red blood cell is placed in a hypotonic solution, _____ will occur.

ANSWER:

Hint 3. Which solutions are hypertonic, isotonic, or hypotonic?

Classify each solution as hypertonic, isotonic, or hypotonic to a red blood cell.

Solution A: 3.21% (m/v) NaClSolution B: 1.65% (m/v) glucoseSolution C: distilled H2OSolution D: 6.97% (m/v) glucoseSolution E: 5.0% (m/v) glucose and 0.9%(m/v) NaCl

Drag each solution to the appropriate bin.

ANSWER:

ANSWER:

crenation / hemolysis

hemolysis / crenation



Correct

This activity shows why it is very important to use solutions that are isotonic to body fluids in intravenous solutions (IVs). If an IV solution werehypertonic to the body fluids, cells in the body would shrink. If an IV solution were hypotonic to the body fluids, cells in the body would swell.


Part A

The volume enclosed by the plasma membrane of plant cells is often much larger than the corresponding volume in animal cells. The most reasonableexplanation for this observation is that

ANSWER:

Correct


Part A

Which of the following statements concerning cells of bacteria and archaea is correct?

ANSWER:

plant cells can have lower surface-to-volume ratios than animal cells because plant cells synthesize their own nutrients.

plant cells have a much more highly convoluted (folded) plasma membrane than animal cells.

plant cells are capable of having a much higher surface-to-volume ratio than animal cells.

animal cells are more spherical, whereas plant cells are elongated.

plant cells contain a large vacuole that reduces the volume of the cytoplasm.



Correct


Part A

If radioactive deoxythymidine triphosphate (dTTP) is added to a culture of rapidly growing bacterial cells, where in the cell would you expect to find thegreatest concentration of radioactivity?

ANSWER:

Correct


Part A

One of the key innovations in the evolution of eukaryotes from a prokaryotic ancestor is the endomembrane system. What eukaryotic organelles orfeatures might have evolved as a part of, or as an elaboration of, the endomembrane system?

ANSWER:

Correct

Tour of an Animal Cell: The Endomembrane System (BioFlix tutorial)

Eukaryotic cells have an integrated network of organelles, including the endoplasmic reticulum (ER), the Golgi apparatus, and lysosomes, which iscollectively referred to as the endomembrane system. The endomembrane system serves a variety of functions within the cell, including protein synthesisand transport, metabolism of lipids, and detoxification.

Before beginning this tutorial, watch the Endomembrane System animation.

Archaea contain small membrane-enclosed organelles; bacteria do not.

Archaea contain a membrane-bound nucleus; bacteria do not.

DNA is present in the mitochondria of both bacteria and archaea.

DNA is present in both archaea and bacteria.

nucleoid

endoplasmic reticulum

ribosomes

cytoplasm

nucleus

chloroplasts

nuclear envelope

mitochondria

plasma membrane

none of these



Part A - Organelles of the endomembrane system

The various parts of the endomembrane system serve different functions in the cell. In this activity, you will identify the roles of each part of theendomembrane system.

Drag each function to the appropriate bin.

Hint 1. Cisternal maturation

Cisternal maturation is the process by which the cisternae of the Golgi apparatus progress from the cis face to the trans face.

Hint 2. Which organelle of the endomembrane system functions in protein synthesis?

The part of the endomembrane system that is studded with ribosomes for protein synthesis is the _____.

ANSWER:

Hint 3. In what ways is the endomembrane system similar to a factory?

The endomembrane system performs so many different functions in the cell that it is often compared to a factory with elaborate assembly linesand shipping capabilities.

Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Not all terms will be used.

ANSWER:

smooth ER

Golgi apparatus

rough ER



ANSWER:



All attempts used; correct answer displayed

The endomembrane system is critical for the synthesis, processing, and movement of proteins and lipids in the cell. The smooth ER functionsmainly in lipid synthesis and processing. The rough ER is the site of secretory protein synthesis. These proteins are processed further in theGolgi apparatus, from where they are dispatched in vesicles to the plasma membrane. Lysosomes, whose enzymes and membranes are madeand processed by the rough ER and Golgi apparatus, function in the hydrolysis of macromolecules, such as in phagocytosis and autophagy.

Part B - Sites of protein synthesis

All proteins are synthesized by ribosomes in the cell. Some ribosomes float freely in the cytosol, while others are bound to the surface of theendoplasmic reticulum. Most proteins made by free ribosomes function in the cytosol. Proteins made by bound ribosomes either function within theendomembrane system or pass through it and are secreted from the cell.Which of the following proteins are synthesized by bound ribosomes?

Select all that apply.


To determine whether a protein is synthesized by free ribosomes or bound ribosomes, be sure to consider where the protein ultimately ends up.In general, there are two classes of proteins:

those that function in the cytoplasm or in environments that are in direct contact with the cytoplasm (Note that although thenucleus is enveloped by two membranes, the interior is still considered to be in direct contact with the cytoplasm via thenumerous pores that allow transport between these two environments.)those that function in locations separated from the cytoplasm by a cellular membrane

The proteins in the first class are synthesized by free ribosomes; they will ultimately function in the cytoplasm or within the nucleus. Theproteins in the second class are synthesized by bound ribosomes; they will ultimately reside in or pass through the endomembrane system.

Hint 2. Where in the cell are nuclear proteins made?

Some proteins must pass into and out of the nucleus to regulate a variety of activities, including the synthesis of DNA, RNA, and ribosomes.

Which of the following pathways would a protein destined for the interior of the nucleus follow?

ANSWER:

ANSWER:

The protein is translated at the surface of the rough ER, is packaged into a vesicle, and the vesicle fuses with the nuclear envelope,depositing the protein inside.

The protein is translated on free ribosomes and then passes through the pore complex to enter the nucleus.

The protein is translated at the surface of the rough ER and then moves from the interior of the ER to the interior of the nucleus.

The protein is translated on free ribosomes and then passes between the lipids of the nuclear envelope to enter the nucleus.



Correct

Most proteins that function in the cytosol (such as actin) or in the nucleus (such as DNA polymerase) are synthesized by free ribosomes.Proteins that function within the endomembrane system (such as lysosomal enzymes) or those that are destined for secretion from the cell (suchas insulin) are synthesized by bound ribosomes.As a protein destined for the endomembrane system is being synthesized by a ribosome, the first amino acids in the growing polypeptide chainact as a signal sequence. That signal sequence ensures that the ribosome binds to the outer membrane of the ER and that the protein enters theER lumen.

Part C - The pathway of secretory proteins

Proteins that are secreted from a eukaryotic cell must first travel through the endomembrane system.

Drag the labels onto the diagram to identify the path a secretory protein follows from synthesis to secretion. Not all labels will be used.


Not all of the labels will be used in the flowchart. Review the functions of each organelle listed and eliminate the organelles not involved in thesynthesis, modification, or transport of secretory proteins. Then place the remaining organelles in the correct order.

Hint 2. Where are the organelles of the endomembrane system located?

Knowing the structure and locations of the compartments that make up the endomembrane system can help you understand how substancesmove within and between the organelles.

Drag the labels to their appropriate locations on the diagram.

ANSWER:

Hint 3. How does cargo move through the Golgi?

As cargo passes through the Golgi apparatus, it is modified in a step-wise manner prior to being transported to the plasma membrane or toother locations in the endomembrane system. These distinct modifications are based on the presence of different Golgi enzymes in eachcisterna.Complete this sentence.

Cargo leaves the endoplasmic reticulum and is sent to the _____ Golgi cisterna, which then matures through the redistribution of enzymes to

lysosomal enzyme

actin

ER protein

insulin

ribosomal protein

DNA polymerase



become a _____ Golgi cisterna and eventually the _____ Golgi cisterna.

ANSWER:

ANSWER:

Correct

As they are being synthesized, secretory proteins enter the lumen of the endoplasmic reticulum. From the ER, vesicles transport these proteinsto the Golgi, where they are sequentially modified and concentrated in a cis-to-trans direction. Secretory vesicles bud from the Golgi and movealong cytoskeletal filaments to eventually fuse with the plasma membrane, secreting their protein cargo. Each of these transport steps requiresspecialized proteins to ensure that the cargo is sent to the proper location and is able to fuse with the target membrane.

Part D - Pulse-chase experiments and protein location

Scientists can track the movement of proteins through the endomembrane system using an approach known as a pulse-chase experiment. Thisexperiment involves

the “pulse” phase: Cells are exposed to a high concentration of a radioactively labeled amino acid for a short period to tag proteins thatare being synthesized.the “chase” phase: Any unincorporated radioactively labeled amino acids are washed away and large amounts of the same, butunlabeled, amino acid are added.

Only those proteins synthesized during the brief pulse phase are radioactively tagged. These tagged proteins can be tracked through the chase periodto determine their location in the cell.

The data below were obtained from a pulse-chase experiment in which cells were examined at different times during the chase period. The numbersrepresent the radioactivity (measured in counts per minute) recorded at each of the indicated sites. The higher the number, the greater the radioactivity.

Based on these data, what is the most likely function of the cells in this experiment?

cis; trans; medial

cis; medial; trans

medial; cis; trans

trans; medial; cis



Hint 1. Understanding the pulse-chase experiment

The pulse-chase experiment is similar to fitting newly synthesized proteins with identification collars, much as one would do in the field to trackanimals. By using a chase, this cohort of newly synthesized proteins can be tracked as they move to different regions of the cell, without anysubsequently synthesized proteins complicating the results. By tracking these proteins over time, the target region where the proteins functioncan be identified based on the concentration of radioactivity.

Hint 2. How can the results of this experiment be represented graphically?

The data from this experiment can be represented in line graphs. The change in radioactivity in each part of the cell has been plotted over time.

Drag a label to the target beneath each graph to indicate which part of the cell the graph represents. Labels can be used once ornot at all.

ANSWER:

Hint 3. What is phagocytosis?

Some cells ingest smaller particles through the process of phagocytosis.Which of the following are true statements about phagocytosis, the vesicles formed during phagocytosis (called phagosomes, or food vacuoles),and the fate of the ingested particles?

Select all that apply.

ANSWER:

ANSWER:

Phagocytosis only occurs in protists.

Phagocytosis occurs when part of the cell’s plasma membrane engulfs a particle, forming a phagosome.

Lysosomes fuse with phagosomes formed during phagocytosis.

Phagosomes by themselves can digest the ingested particles into molecules the cell can use.

The contents of phagosomes are digested with the help of hydrolytic enzymes in lysosomes.



Correct

The cells in this experiment were macrophages. These immune system cells have many lysosomes for the destruction of bacteria and otherinvaders brought into the cell via phagocytosis. The enzymes (hydrolases) that carry out this catabolic activity are synthesized in the endoplasmicreticulum, modified in the Golgi, and transported to the lysosomes.


Part A

Large numbers of ribosomes are present in cells that specialize in producing which of the following molecules?

ANSWER:

Correct

Activity: Cilia and Flagella



Part A

The cilia and flagella of eukaryotic cells are composed of _____.

ANSWER:

detoxification

muscle contraction

phagocytosis

secretion

proteins

cellulose

glycogen

lipids

nucleic acids

intermediate filaments

tonofilaments

microfilaments

pili

microtubules





Correct

Eukaryotic cilia and flagella are composed of microtubules.


Part A

Movement of vesicles within the cell depends on what cellular structures?

ANSWER:

Correct


Part A

Which of the following functions is NOT associated with the cytoskeleton in eukaryotic cells?

Hint 1.

The cytoskeleton is a network of fibers that is involved in structural organization and motion.

ANSWER:

Correct

RNA molecules exit the nucleus via nuclear pores.


Part A

Which of the following statements about a typical plasma membrane is correct?

Hint 1.

Consider the composition, structure, and function of biological membranes.

ANSWER:

actin filaments and motor proteins

actin filaments and ribosomes

actin filaments and microtubules

centrioles and motor proteins

microtubules and motor proteins

maintaining the position of the nucleus in the cell

determining the shape of animal cells

the contraction of muscle cells in animals

the beating of cilia or flagella

Movement of RNA molecules from the nucleus to the cytoplasm



Correct

Because the membrane serves different functions on the cytoplasmic and exterior surfaces, the structure and composition of the surfaces mustbe different.


Part A

Which of the following best describes the structure of a biological membrane?

Hint 1.

Consider how the molecular components of a biological membrane are organized.

ANSWER:

Correct

The membrane proteins can be found either embedded in or attached to the surface of the phospholipid bilayer.

Activity: Diffusion and Osmosis

Click here to view this animation.


Part A

Which of the following factors does not affect membrane permeability?

Hint 1.

What factors allow some molecules to pass through a membrane more readily than others?

The hydrophilic interior of the membrane is composed primarily of the fatty acid tails of the phospholipids.

The two sides of the plasma membrane have different lipid and protein composition.

The plasma membrane is a covalently linked network of phospholipids and proteins that controls the movement of solutes into and out of acell.

Carbohydrates on the membrane surface are important in determining the overall bilayer structure.

Phospholipids are the primary component that determines which solutes can cross the plasma membrane.

a fluid structure in which phospholipids and proteins move freely between sides of the membrane

two layers of phospholipids with proteins embedded between the two layers

a mixture of covalently linked phospholipids and proteins that determines which solutes can cross the membrane and which cannot

two layers of phospholipids (with opposite orientations of the phospholipids in each layer) with each layer covered on the outside withproteins

two layers of phospholipids with proteins either crossing the layers or on the surface of the layers





ANSWER:

Correct

Phospholipids contain both a polar head and a nonpolar hydrocarbon tail, both of which are necessary for their ability to form membrane bilayers.

Part B

How can a lipid be distinguished from a sugar?

Hint 1.

What is the definition of a lipid?

ANSWER:

Correct

Lipids are nonpolar molecules, whereas sugars are polar.

Part C

True or false? Osmosis is a type of diffusion.

Hint 1.

What process occurs during osmosis?

ANSWER:

Correct

Osmosis is the diffusion of water across a selectively permeable membrane.

Part D

What property of dishwashing liquid (detergent) makes it useful to wash grease from pans?

Hint 1.

Think about how oils can be isolated from water.

ANSWER:

The amount of cholesterol in the membrane

The polarity of membrane phospholipids

Temperature

The saturation of hydrocarbon tails in membrane phospholipids

A lipid dissolves in water.

Lipids are mostly nonpolar.

A lipid is made up of only hydrocarbons.

Lipids are mostly saturated.

True

False



Correct

Detergents form micelles around the grease, which are then washed away because the polar head groups facing outward on the micelle are water-soluble.

Part E

Which of the following particles could diffuse easily through a cell membrane?

Hint 1.

Remember that small nonpolar molecules pass most easily through cell membranes.

ANSWER:

Correct

Small nonpolar molecules such as oxygen can diffuse across cell membranes.

Part F

True or false? The water-soluble portion of a phospholipid is the polar head, which generally consists of a glycerol molecule linked to a phosphategroup.

Hint 1.

Think about the orientation of the phospholipids that make up a cell membrane.

ANSWER:

Correct

The hydrophilic, or water-loving, portion of a phospholipid is the polar head, whereas the hydrophobic portion is the nonpolar tail.

Part G

If a red blood cell is placed in a salt solution and bursts, what is the tonicity of the solution relative to the interior of the cell?

Hint 1.

Consider the movement of water when the solute concentration inside the cell is different from that of the surrounding environment.

ANSWER:

Permeability

Amphipathic nature

Hydrophobic nature

Solubility in water

Hydrogen ion (H+)

Sodium ion (Na+)

Oxygen (O2)

Glucose

True

False



Correct

The salt concentration in the solution is lower than it is in the cell, so water enters the cell, causing it to burst.

Activity: Membrane Transport Proteins

Click here to view this animation.


Part A

What distinguishes facilitated diffusion from simple diffusion?

Hint 1.

What is facilitated diffusion?

ANSWER:

Correct

Membrane proteins facilitate the movement of molecules across the membrane.

Part B

Which of the following transporters does not use passive transport to move molecules across a membrane?

Hint 1.

Which of following transporters uses energy?

ANSWER:

Hypertonic

Hypotonic

Isotonic

Osmotic

Membrane proteins help move molecules across the membrane.

No energy is used to move molecules across the membrane.

Molecules move from a region of higher concentration to a region of lower concentration.

Molecules move from a region of lower concentration to a region of higher concentration.





Correct

This pump uses the energy provided by ATP hydrolysis to transport sodium and potassium ions across the membrane.

Part C

True or false? Active transport differs from passive transport in that active transport does not require an input of energy.

Hint 1.

What is the definition of active transport?

ANSWER:

Correct

Active transport requires the input of energy, whereas passive transport is primarily driven by the gradient.Active transport requires energy,whereas passive transport does not.

Part D

Which membrane protein would be used to move glucose across a membrane from a region of higher concentration to a region of lower concentration?

Hint 1.

Consider what type of molecule is being transported under these conditions.

ANSWER:

Correct

The GLUT-1 transporter assists the diffusion of glucose through the membrane.

Part E

Which of the following statements is true about the net movement of an ion across a membrane by passive diffusion through a membrane channel?

Hint 1.

Consider the requirements that must be met for passive diffusion to occur.

ANSWER:

Valinomycin

GLUT-1 glucose transporter

Gramicidin ion channel

Sodium–potassium pump

True

False

Ion channel

Pump

Transporter

No protein is necessary.



Correct

Ions tend to move toward regions of unlike charge.

Part F

True or false? During active transport through the sodium-potassium pump, ATP hydrolysis provides the energy to pump three sodium ions into the cellfor every two potassium ions pumped out of the cell.

Hint 1.

The concentration gradients of the ions are a form of potential energy that can be used to perform work.

ANSWER:

Correct

The sodium-potassium pump maintains a state in which the concentration of sodium is low inside the cell relative to the outside and theconcentration of potassium is high inside the cell relative to the outside; thus, three sodium ions are pumped out of the cell and two potassiumions are pumped into the cell against the electrochemical gradient.

Part G

Lactose transport by membrane proteins occurs under conditions in which the concentration of lactose inside the cell is higher than the concentrationoutside the cell. What type of transport is used to move lactose into the cell?

Hint 1.

Consider how lactose moves in relation to its concentration gradient.

ANSWER:

Correct

Under these conditions, lactose moves against its concentration gradient, so its transport will require energy.

Score Summary:

Your score on this assignment is 89.2%.You received 15.16 out of a possible total of 17 points.

For every ion that diffuses through the membrane, a different ion diffuses in the opposite direction.

The cell expends energy as the ion passes through the membrane.

There is a higher concentration of ions on the side of the membrane toward which the ion diffuses.

The charge on the other side of the membrane is generally the opposite of the ion's charge.

True

False

Simple diffusion

Facilitated diffusion

Osmosis

Active transport

chapter 6 - tour of the cell

Documents

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