chem 509 signal transduction winter 2010 lecture 2
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Chem 509Signal Transduction
Winter 2010
Lecture 2
What difference between Gs, Gq and Gi?:
1. Gs, G protein that is stimulatory and binds AC2. Gq, G protein that is stimulatory and binds PLC3. Gi, G protein that is inhibitory and binds AC.
O
H
H
O
OH
HH
OH
OH
OH HP
O
O
O-
H2C
CHOC
O
R2
H2C O C
O
R1 P-O
O-
O
P
O
O-
O-
Phosphatidylinositol
O
H
H
OH
OH
HH
OH
OHH
OH HP
O
O
O-
H2C
CHOC
O
R2
H2C O C
O
R1
Phosphatidylinositol-diphosphate
Phospholipases
Phospholipase A1-can hydrolyse the sn-1 acyl bond but can alsohydrolyse the sn-2 acyl bond. In the former case, this liberates a fatty acid and 2-acyl-glycerophospholipid.
O
H
H
O
OH
HH
OH
OH
OH HP
O
O
O-
H2C
CHOC
O
R2
H2C O C
O
R1 P-O
O-
O
P
O
O-
O-
O
H
H
O
OH
HH
OH
OH
OH HP
O
O
O-
H2C
CHOC
O
R2
H2C O C
O
R1 P-O
O-
O
P
O
O-
O-
Phospholipases
Phospholipase A 2-hydrolyses the sn-2 acyl bond. This liberates a fatty acid and a lysophospholipid (1-acyl-glycerophospholipid)
O
H
H
O
OH
HH
OH
OH
OH HP
O
O
O-
H2C
CHOC
O
R2
H2C O C
O
R1 P-O
O-
O
P
O
O-
O-
Phospholipases
Phospholipase C-hydrolizes one phosphoester bond to generate diacyglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3).
O
H
H
O
OH
HH
OH
OH
OH HP
O
O
O-
H2C
CHOC
O
R2
H2C O C
O
R1 P-O
O-
O
P
O
O-
O-
Phospholipases
Phospholipase D-will hydrolyse the other phosphoester bond to generate phosphatidic acid (amphiphilic product) and inositol 4,5 diphosphate (IP2).
PI=Phosphatidylinositol, Phosphorylated derivatives of PI are denoted as PIPx.PC=Phosphatidylcholine
PA=Phosphatidic acidLyso PC= deacylated phosphatidylcholine
Calmodulin
End of GPCR lecture story
Insulin signaling
Insulin signaling
Other Receptor Tyrosine Kinase Receptors
Cross talk (part of integration of signals)
Brief overview of cancer signaling pathways
Cell cycle
