cloud object storage | store & retrieve data …€¦ · web viewnguyen tan phata,*, tran thi...
TRANSCRIPT
SUPPLEMENTARY MATERIAL
New C20-gibberellin diterpene from the leaves of Schefflera sessiliflora De. P. V.
Nguyen Tan Phata,*, Tran Thi Thao Vyb, Mai Dinh Tria, Le Tien Dunga, Phan Nhat Minha, Bui
Trong Data
aInstitute of Chemical Technology, Vietnam Academy of Science and Technology, Ho Chi Minh
city, Viet Nam bUniversity of Technology, Vietnam National University, Ho Chi Minh city, Viet Nam
Corresponding author. Phone: +84 916 360 751 Email: [email protected] or
Asbract: From the leaves of Schefflera sessiliflora De P. V., one new C20-gibberellin diterpene
2β,12β-dihydroxygibberellin (12β-hydroxy-GA110 or 2β-hydroxy-GA112) (1); together with 3
known compounds, trans-tiliroside (2), kaempferol 3-O-β-D-glucuronopyranoside (3), 5-p-trans-
coumaroylquinic acid (4) were isolated for the first time from the genus Schefflera by various
chromatography methods. Their structures were elucidated by IR, UV, HR-ESI-MS, NMR 1D
and 2D experiments and comparison with previous reported data. The α-glucosidase inhibitory
activity of all compounds were measured. The isolates (2, 3) showed better α-glucosidase
inhibitory activity (IC50 = 134.60, 147.10 μM, respectively) than the standard drug acarbose (IC50
= 214.50 μM).
Keywords: Schefflera sessiliflora De P. V.; Araliaceae; gibberellin; diterpene; α-glucosidase
inhibition
List of supporting information
Table S1. α-Glucosidase inhibition of compounds 1-4.
Figure S1. Chemical structures and selected HMBC, COSY and ROESY correlations of 1
Figure S2. 1H-NMR spectrum (500 MHz) of compound 1 in CD3OD.
Figure S3. 13C-NMR spectrum (125 MHz) of compound 1 in CD3OD.
Figure S4. DEPT spectrum of compound 1 in CD3OD.
Figure S5. COSY spectrum of compound 1 in CD3OD.
Figure S6. HSQC spectrum of compound 1 in CD3OD.
Figure S7. HMBC spectrum of compound 1 in CD3OD.
Figure S8. ROESY spectrum of compound 1 in CD3OD.
Figure S9. HR-ESI-MS spectrum of compound 1 in CD3OD.
Table S1. α-Glucosidase inhibition of compounds 1-4.
-
Inhibition < 1%
No. SamplesInhibition (%)
IC50 (µM)250 µM 100 µM 50 µM 25 µM 10 µM
1 1 41.50 ± 2.20 13.81 ± 0.94 - - - > 250.002 2 76.40 ± 0.82 42.10 ± 1.90 10.53 ± 0.53 - - 134.603 3 96.88 ± 0.74 28.60 ± 1.20 - - - 147.104 4 31.33 ± 0.90 18.30 ± 1.90 9.60 ± 1.60 - - > 250.005 Acarbose 214.50
A B
1Figure S1. Chemical structures and selected HMBC, COSY and ROESY correlations of 1
Figure S2. 1H-NMR spectrum (500 MHz) of compound 1 in CD3OD.
Figure S3. 13C-NMR spectrum (125 MHz) of compound 1 in CD3OD.
Figure S4. DEPT spectrum of compound 1 in CD3OD.
Figure S5. COSY spectrum of compound 1 in CD3OD.
Figure S6. HSQC spectrum of compound 1 in CD3OD.
Figure S7. HMBC spectrum of compound 1 in CD3OD.
Figure S8. ROESY spectrum of compound 1 in CD3OD.
Figure S9. HR-ESI-MS spectrum of compound 1 in CD3OD.