Coleus Forskholii

Scientific classification Kingdom: Plantae(unranked): Angiosperms(unranked): Eudicots(unranked): AsteridsOrder: LamialesFamily: LamiaceaeGenus: Solenostemon

Solenostemon is a genus of perennial plants, native to tropical Africa, Asia, Australia, the East Indies, the Malay Archipelago, and the Philippines. They are commonly known as Coleus, a name which derives from an earlier classification under the genus name Coleus, which is currently treated as two: with species included in either the genus Solenostemon or in another genus, Plectranthus.

Cultivation

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Many cultivars of the southeast Asian species Solenostemon scutellarioides have been selected for their colorful variegated leaves, typically with sharp contrast between the colors; the leaves may be green, pink, yellow, black (a very dark purple), maroon, and red (somewhat resembling the unrelated caladium). New cultivars with varieties of colors are constantly being made. The plants grow well in moist well-drained soil, and typically grow 0.5-1 m tall, though some may grow as tall as 2 meters. Coleus is typically grown as ornamental plants. They are heat-tolerant, though they do less well in full sun in subtropical areas than in the shade. In mild areas (no snow in winter), plants can usually be kept as perennials if well managed. Whereas cultivation in colder areas, they are often grown as annuals as they are not hardy and become leggy and unattractive with age. Though this is usually resolved by pinching back growing tips to encourage bushing. In bright hot areas, the colors of the plant will typically be more intense in shaded areas than in full sun, and the plants will require less water there. Coleus also make low-maintenance houseplants, and can often be propagated by clipping a length of stem just below the leaves and putting the stem in water to root. The plant's flowers grow on a stem above that stem's leaves, and tend to be purple and quite small in comparison to the leaves. The plant is not generally grown for its flowers, as it promotes stem elongation (remove to halt this).

Diseases

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One disease that can affect coleus is downy mildew. This mildew appears on the leaves making the plant look dirty because it is brown in color. The organism is called Peronospora sp. and can also result in curled and twisted leaves. Sometimes symptoms are not found on leaves which make the disease harder to control.[1] Another disease is Impatiens necrotic spot virus which causes brown or yellow spots on leaves, rings, black or brown stem discoloration, and brown leaf veins, ultimately resulting in plant death. The disease is spread by an insect called a thrips that carries the virus from an infected plant to an uninfected plant. It only takes a few

of these insects to infect a whole greenhouse. [2]

Propagation

There are two ways to propagate Coleus. Seeds are inexpensive and easily obtainable. To germinate, simply sprinkle seeds on the surface soil and press down. Seeds need light to germinate, so avoid covering the seeds. To keep seeds moist, grow in a container and cover with plastic, or mist seeds daily (if starting seeds directly in the garden). Sprouts can show color in as little as two weeks. Alternatively, cuttings can be taken.

Cuttings root readily in plain water, without the addition of rooting hormone (although it is still beneficial).

Biosynthesis

As with other members of the large diterpene family of natural products, forskolin is derived from geranylgeranyl pyrophosphate (GGPP). Forskolin, however, contains some unique functional elements, key among them is the presence of a tetrahydopyran derived heterocyclic ring. This ring is synthesized after the formation of the trans-fused carbon ring systems are formed by a

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carbocation mediated cyclization. The remaining tertiary carbocation is quenched by molecule of water. After deprotonation, the remaining hydroxyl group is free to form the heterocyclic ring. This cyclization can occur either by attack of the alcohol oxygen onto the allylic carbocation formed by loss of diphosphate, or by an anlagous SN2' like displacement of the diphosphate.[1] This forms the core ring system A of forskolin.

The remaining modifications of the core ring system A can putatively be understood as a series of oxidation reactions to form a polyol B which is then further oxidized and esterified to form the ketone and acetate ester moieties seen in forskolin. However, because the biosynthetic gene cluster has not been described this putative synthesis could be incorrect in the sequence of oxidation/esterification events, which could occur in almost any order.

Potential medical use

Applied with rolipram, forskolin provides a route to inhibition of colon cancer cell growth and survival. [2] These two drugs also work together to induce long-term potentiation in neuronal populations. [3]

Forskolin is a vasodilator. To date, there have been more than two clinical studies examining

the effectiveness of forskolin as a weight loss aid. Only one has been subject to peer-review and published in a medical journal. This clinical study also observed forskolin's role in significantly increasing lean mass, bone mass, and testosterone in the subjects involved.[4] This research has led to companies marketing forskolin as a bodybuilding supplement.

Forskolin may be helpful to control the underlying cause of glaucoma. The sometimes successful use of forskolin to reduce intraocular pressure may be due to its unique ability to stimulate adenylate cyclase activity and increase cAMP which regulates and activates critical enzymes required for the cellular energy required to move fluid out of the eye.

Increase skin's natural resistance to burning under UV light (see links below)

Stimulate a tanning response when applied topically. Reduce urinary tract infections and enhance the ability of

antibiotics to kill bacteria that normally survive.

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Forskolin can also be used to promote nerve repair by increasing cAMP concentrations. Forskolin can activate or upregulate the proliferation of Schwann cells in culture, together with Fibroblast growth factor or Transforming Growth Factor-Beta.

Various experimental studies are underway in using Forskolin as an adjuvant in treatment for diseases such as Parkinsons and/or nerve damage caused by trauma/accident.

Coleus Forskohlii Side Effects

Coleus Forskohlii is generally safe with little to no side effects. The studies done on Coleus Forskohlii extract reported no adverse side effects. However, these are just a small number of studies. Coleus Forskohlii may lower blood pressure - anyone with low or high blood pressure, or cardiovascular diseases should be cautious about using it.

Aloe vera

Scientific classification Kingdom: PlantaeOrder: AsparagalesFamily: AsphodelaceaeGenus: AloeSpecies: Aloe veraBinomial name Aloe vera

Aloe vera, also known as the true or medicinal aloe, is a species of succulent plant that probably originated in the southern half of the Arabian peninsula, Northern Africa, the Canary islands and Cape Verde. Aloe vera grows in arid climates and is widely distributed in Africa, India and other arid areas. The species is frequently cited as being used in herbal medicine. Many scientific studies of the use of aloe vera have been undertaken, some of them conflicting.[1][2][3][4] Despite these limitations, there is some preliminary evidence that Aloe vera extracts may be useful

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in the treatment of wound and burn healing, minor skin infections, Sebaceous cyst, diabetes and elevated blood lipids in humans.[3] These positive effects are thought to be due to the presence of compounds such as polysaccharides, mannans, anthraquinones and lectins.[3][5][6]

Description

Aloe vera is a stemless or very short-stemmed succulent plant growing to 60–100 cm (24–39 in) tall, spreading by offsets. The leaves are thick and fleshy, green to grey-green, with some varieties showing white flecks on the upper and lower stem surfaces.[7] The margin of the leaf is serrated and has small white teeth. The flowers are produced in summer on a spike up to 90 cm (35 in) tall, each flower pendulous, with a yellow tubular corolla 2–3 cm (0.8–1.2 in) long.[7][8] Like other Aloe species, Aloe vera forms arbuscular mycorrhiza, a symbiosis that allows the plant better access to mineral nutrients in soil.[9]

Taxonomy and etymology

The species has a number of synonyms: A. barbadensis Mill., Aloe indica Royle, Aloe perfoliata L. var. vera and A. vulgaris Lam.,[10][11] and common names including Chinese Aloe, Indian Aloe, true Aloe, Barbados Aloe, burn Aloe, first aid plant.[8][12][13][14][15] The species name vera means "true" or "genuine."[12] Some literature identifies the white spotted form of Aloe vera as Aloe vera var. chinensis,[16][17] however, the species varies widely with regard to leaf spots [18] and it has been suggested that the spotted form of Aloe vera may be conspecific with A. massawana.[19] The species was first described by Carl Linnaeus in 1753 as Aloe perfoliata var. vera,[20] and was described again in 1768 by Nicolaas Laurens Burman as Aloe vera in Flora Indica on the 6th of April and by Philip Miller as Aloe barbadensis some ten days after Burman in the Gardener's Dictionary.[21]

Techniques based on DNA comparison suggest that Aloe vera is relatively closely related to Aloe perryi, a species that is endemic to Yemen.[22] Similar techniques, using chloroplast DNA sequence comparison and ISSR profiling have also suggested that Aloe vera is closely related to Aloe forbesii, Aloe inermis, Aloe scobinifolia, Aloe sinkatana and Aloe striata.[23] With the exception of South African species, A. striata, these Aloe species are native to Socotra (Yemen), Somalia and Sudan.[23] The lack of obvious natural populations of the

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species have led some authors to suggest that Aloe vera may be of hybrid origin.[24]

Distribution

The natural range of Aloe vera is unclear, as the species has been widely cultivated throughout the world. Naturalised stands of the species occur in the southern half of the Arabian peninsula, through North Africa, from Morocco to Egypt and Sudan, along with the Canary, Cape Verde and Madeira Islands.[10] This distribution is similar to the one of Euphorbia balsamifera, Pistacia atlantica and a few others, suggesting that a dry sclerophyl forest once covered large areas, but has been dramatically reduced due to desertification in the Sahara, leaving these few patches isolated. The species was introduced to China and various parts of southern Europe in the 17th century.[25] The species is widely naturalised elsewhere, occurring in temperate and tropical regions of Australia, Barbados, Belize, Nigeria, Paraguay and the US [18] [26] It has been suggested that the actual species' distribution is the result of human cultivation and that the taxonomy could be doubtful too.[19]

Alternative names

- In India, the plant is known as Korphad inMaharashtra, Ghrtakumarior Gheekvar and is sometimes used in Ayurvedic healing. In states of Rajasthan and Gujarat it is known as GwarPatha, In Tamilnadu it is known as Katralai

- In Pakistan, the plant is known as Quargandal and is used in Unani (Greek-Islamic) medicine.

- In South America it is known as Sabila.

- In Indonesia, it is known as Lidah Buaya.

- In Thailand, it is known as the "Crocodile Tail" plant.

- In many parts of India the plant extract is used as a home remedy for numerous skin allergies, acne, fungus infections and beauty-aid. In the state of Kerala, where it is known as kattar vazha, Aloe Vera is a common household name and for a long time it is being used for Ayurvedic treatment. And in the state of Tamil nadu, Aloe vera is known as katraazhai and it has also a pet name kumari. The pulp of the plant is highly regarded for its anti-ageing potential, hence the name kumari. The pulp is used extensively in Siddha medicines for treating constipation,

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enlargement of spleen, zymotic disease, chengamaari (a type of venereal infection) etc.[27]

-In Mexico and Latin America it is often referred to as either "Savia", "Savila", or "Sabila." It is widely used both by Indigenous Peoples as well as by some Mestizos as a traditional medicine.

Cultivation

Aloe vera has been widely grown as an ornamental plant. The species is popular with modern gardeners as a putatively medicinal plant and due to its interesting flowers, form and succulence. This succulence enables the species to survive in areas of low natural rainfall, making it ideal for rockeries and other low-water use gardens.[7] The species is hardy in zones 8–11, although it is intolerant of very heavy frost or snow.[8][28] The species is relatively resistant to most insect pests, though spider mites, mealy bugs, scale insects and aphid species may cause a decline in plant health.[29][30] In pots, the species requires well-drained sandy potting soil and bright sunny conditions; however, in very hot and humid tropical or subtropical climates, aloe plants should be protected from direct sun and rain, as they will burn and/or turn mushy easily under these conditions. The use of a good quality commercial propagation mix or pre-packaged "cacti and succulent mix" is recommended as they allow good drainage.[31] Terracotta pots are preferable as they are porous.[31] Potted plants should be allowed to completely dry prior to re-watering. When potted aloes become crowded with "pups" growing from the sides of the "mother plant," they should be divided and re-potted to allow room for further growth and help prevent pest infestations. During winter, A. vera may become dormant, during which little moisture is required. In areas that receive frost or snow the species is best kept indoors or in heated glasshouses.[8] Large scale agricultural production of Aloe vera is undertaken in Australia,[32] Bangladesh, Cuba,[33] the Dominican Republic, China, Mexico,[34] India,[35] Jamaica,[36] Kenya and South Africa,[37] along with the USA[38] to supply the cosmetics industry with Aloe vera gel.

Medicinal uses

Scientific evidence for the cosmetic and therapeutic effectiveness of Aloe vera is limited and when present is typically contradictory.[1][2] Despite this, the cosmetic and alternative medicine industries regularly make

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claims regarding the soothing, moisturising and healing properties of Aloe vera, especially via Internet advertising.[3][39][40][41][42] Aloe vera gel is used as an ingredient in commercially available lotion, yogurt, beverages and some desserts.[43][44][45] Aloe vera juice is used for consumption and relief of digestive issues such as heartburn and irritable bowel syndrome. It is common practice for cosmetic companies to add sap or other derivatives from Aloe vera to products such as makeup, tissues, moisturizers, soaps, sunscreens, incense, razors and shampoos.[43] Other uses for extracts of Aloe vera include the dilution of semen for the artificial fertilization of sheep,[46] use as fresh food preservative,[47] and use in water conservation in small farms.[48]

Aloe vera has a long association with herbal medicine, although it is not known when its medical applications were first discovered. Early records of Aloe vera use appear in the Ebers Papyrus from 16th century BCE,[15]

in both Dioscorides' De Materia Medica and Pliny the Elder's Natural History written in the mid-first century CE[15] along with the Juliana Anicia Codex produced in 512 CE.[43] Aloe vera is non-toxic, with no known side effects, provided the aloin has been removed by processing. Taking Aloe vera that contains aloin in excess amounts has been associated with various side effects.[3][4][49] However, the species is used widely in the traditional herbal medicine of China, Japan, Russia, South Africa, the United States, Jamaica and India.[3]

Aloe vera is alleged to be effective in treatment of wounds.[ Evidence on the effects of Aloe vera sap on wound healing, however, is limited and contradictory.] Some studies, for example, show that Aloe vera promotes the rates of healing, while in contrast, other studies show that wounds to which Aloe vera gel was applied were significantly slower to heal than those treated with conventional medical preparations.[ A more recent review (2007) concludes that the cumulative evidence supports the use of Aloe vera for the healing of first to second degree burns.[ In addition to topical use in wound or burn healing, internal intake of Aloe vera has been linked with improved blood glucose levels in diabetics,[ and with lower blood lipids in hyperlipidaemic patients,[ but also with acute hepatitis (liver disease).[ In other diseases, preliminary studies have suggested oral Aloe vera gel may reduce symptoms and inflammation in patients with ulcerative colitis. Compounds extracted from Aloe vera have been used as an immunostimulant that aids in fighting cancers in cats and dogs;[ however, this treatment has not been scientifically tested in humans. The injection of Aloe vera extracts to treat cancer has resulted in the deaths of several patients.[

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Topical application of Aloe vera may be effective for genital herpes and psoriasis.[ However, it is not effective for the prevention of radiation-induced injuries. Although anecdotally useful, it has not been proven to offer protection from sunburn or suntan. In a double-blind clinical trial the group using an Aloe vera containing dentifrice and the group using a fluoridated dentifrice both demonstrated a statistically significant reduction of gingivitis and plaque.[

Aloe vera extracts have antibacterial and antifungal activities, which may help in the treatment of minor skin infections, such as boils and benign skin cysts. Aloe vera extracts have been shown to inhibit the growth of fungi that cause tinea;[ however, evidence for control beneath human skin remains to be established. For its anti-fungal properties, Aloe vera is used as a fish tank water conditioner. For bacteria, inner-leaf gel from Aloe vera was shown to inhibit growth of Streptococcus and Shigella species in vitro.[64] In contrast, Aloe vera extracts failed to show antibiotic properties against Xanthomonas species.[65]

Commodity uses

Aloe vera is now widely used on face tissues, where it is promoted as a moisturiser and/or anti-irritant to reduce chafing of the nose of users who suffer hay-fever or cold.[66] It has also been suggested that biofuels could be obtained from Aloe vera seeds.[67] It can also be used to retwist dreadlocked hair, a favourite agent for vegans and those who prefer natural products. To add Aloe Vera is also used for soothing the skin, and keeping the skin moist while eliminating the risk of flaky scalp and skin in harsh and dry weather.

Historical uses

Aloin was the common ingredient in OTC laxative products in the United States prior to 2003, when the FDA ruled that aloin was a class III ingredient, therefore banning its use.[68] It should be noted that processed aloe that contains aloin is used primarily as a laxative, whereas processed aloe vera juice that does not contain significant amounts of aloin is used as a digestive healer. Manufacturers commonly remove aloin in processing due to the FDA ruling.

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Culinary uses

Aloe is also used as a foodstuff. Some molecular gastronomists have begun to take advantage of its gelling properties. Perhaps most notably among these is Chef Quique Dacosta's "Oysters Guggenheim," created at El Poblet in Spain.[

Turmeric

Scientific classification Kingdom: PlantaeOrder: ZingiberalesFamily: ZingiberaceaeGenus: CurcumaSpecies: C. longaBinomial name Curcuma longa

Turmeric (Curcuma longa) is a rhizomatous herbaceous perennial plant of the ginger family, Zingiberaceae.[2] It is native to tropical South Asia and needs temperatures between 20 °C and 30 °C, and a considerable amount of annual rainfall to thrive.[3] Plants are gathered annually for

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their rhizomes, and re-seeded from some of those rhizomes in the following season.

The rhizomes are boiled for several hours and then dried in hot ovens, after which they are ground into a deep orange-yellow powder commonly used as a spice in curries and other South Asian and Middle Eastern cuisine, for dyeing, and to impart color to mustard condiments. Its active ingredient is curcumin and it has a distinctly earthy, slightly bitter, slightly hot peppery flavor and a mustardy smell.

In medieval Europe, turmeric became known as Indian Saffron, since it was widely used as an alternative to the far more expensive saffron spice.[4]

Erode, a city in the south Indian state of Tamil Nadu, is the world's largest producer and most important trading center of turmeric in Asia. For these reasons, Erode in history is also known as "Yellow City" [citation

needed] or "Turmeric City".[citation needed] Sangli, a town in the southern part of the Indian western state of Maharashtra, is the second largest and most important trading center for turmeric in Asia.

Nomenclature and taxonomy

Kingdom | Plantae (plants), Phylum | Magnoliophyta (flowering plants), Class | Liliopsida (monoctyledons), Order | Zingiberales (gingers, bananas, birds-of-paradise, heliconias, costus, cannas, prayer plants, arrowroots, ...), Family | Zingiberaceae (ginger family), Genus | Curcuma (curcuma), Species | Curcuma longa (common turmeric),

Composition

Curcumin Keto form Curcumin Enol form

Turmeric contains up to 5% essential oils and up to 5% curcumin, a polyphenol. It is the active substance of turmeric and it is also known as C.I. 75300, or Natural Yellow 3. The systematic chemical name is (1E,6E)-1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione.

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It can exist at least in two tautomeric forms, keto and enol. The keto form is preferred in solid phase and the enol form in solution.

Curcumin is a pH indicator. In acidic solutions (pH <7.4) it turns yellow whereas in basic (pH > 8.6) solutions it turns bright red.

Culinary uses

Turmeric grows wild in the forests of Southeast Asia. It has become the key ingredient for many Indian, Persian, Thai and Malay dishes, not only in curry, but also in masak lemak, rendang and many more.

In Indonesia, the turmeric leaves are used for Minangese or Padangese curry base of Sumatra such as rendang, sate padang and many other varieties.

Although most usage of turmeric is in the form of root powder, in some regions (especially in Maharashtra), leaves of turmeric are used to wrap and cook food. This usually takes place in areas where turmeric is grown locally, since the leaves used are freshly picked. This imparts a distinct flavor.

In non-South Asian recipes, turmeric is sometimes used as an agent to impart a rich, custard-like yellow color. It is used in canned beverages and baked products, dairy products, ice cream, yogurt, yellow cakes, orange juice, biscuits, popcorn color, sweets, cake icings, cereals, sauces, gelatins, etc.[citation needed] It is a significant ingredient in most commercial curry powders. Turmeric is mostly used in savory dishes, as well as some sweet dishes such as the cake Sfouf.

Although usually used in its dried, powdered form, turmeric is also used fresh, much like ginger. It has numerous uses in Far Eastern recipes, such as fresh turmeric pickle which contains large chunks of soft turmeric.

Turmeric (coded as E100 when used as a food additive)[5] is used to protect food products from sunlight. The oleoresin is used for oil-containing products. The curcumin/polysorbate solution or curcumin powder dissolved in alcohol is used for water-containing products. Over-coloring, such as in pickles, relishes, and mustard, is sometimes used to compensate for fading.

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In combination with annatto (E160b), turmeric has been used to color cheeses, yogurt, dry mixes, salad dressings, winter butter and margarine. Turmeric is also used to give a yellow color to some prepared mustards, canned chicken broths and other foods (often as a much cheaper replacement for saffron).

Turmeric is widely used as a spice in South Asian and Middle Eastern cooking. Many Persian dishes use Turmeric, for the coloring of rice bottoms as well as a starter ingredient for almost all Iranian fry ups (which typically consist of oil, onions and turmeric followed by any other ingredients that are to be included). In Nepal, turmeric is widely grown and is extensively used in almost every vegetable and meat dish in the country for its color as well as for its medicinal value. In South Africa turmeric is traditionally used to give boiled white rice a golden color.

Uses in folk medicine

In Ayurvedic practices, turmeric has many medicinal properties and many in South Asia use it as a readily available antiseptic for cuts, burns and bruises. It is also used as an antibacterial agent.

It is taken in some Asian countries as a dietary supplement, which allegedly helps with stomach problems and other ailments. It is popular as a tea in Okinawa, Japan. Pakistanis also use it as an anti-inflammatory agent, and remedy for gastrointestinal discomfort associated with irritable bowel syndrome, and other digestive disorders. In Afghanistan and northwest Pakistan, turmeric is applied to a piece of burnt cloth, and placed over a wound to cleanse and stimulate recovery. Indians, in addition to its Ayurvedic properties, use turmeric in a wide variety of skin creams that are also exported to neighboring countries.

Preliminary medical research

Turmeric is currently being investigated for possible benefits in Alzheimer's disease,[6] cancer, arthritis, and other clinical disorders.[7]

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In the latter half of the 20th century, curcumin was identified as responsible for most of the biological effects of turmeric. According to a 2005 article in the Wall Street Journal, research activity into curcumin and turmeric is increasing, with supplement sales increased 35% from 2004. The U.S. National Institutes of Health currently has registered 19 clinical trials underway to study use of dietary turmeric and curcumin for a variety of clinical disorders (dated February 2010).[8]

Cosmetics

Turmeric is currently used in the formulation of some sunscreens.[citation

needed] Turmeric paste is used by some Indian women to keep them free of superfluous hair. Turmeric paste is applied to bride and groom before marriage in some places of India, Bangladesh, and Pakistan, where it is believed turmeric gives glow to skin and keeps some harmful bacteria away from the body.

The government of Thailand is funding a project to extract and isolate tetrahydrocurcuminoids (THC) from turmeric. THCs are colorless compounds that might have antioxidant and skin-lightening properties and might be used to treat skin inflammations, making these compounds useful in cosmetics formulations.

Dye

However, turmeric is commonly used in Indian clothing, such Turmeric makes a poor fabric dye as it is not very lightfast (the degree to which a dye as saris.

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Gardening

Turmeric can also be used to deter ants. The exact reasons why turmeric repels ants is unknown, but anecdotal evidence suggests it works.

Ceremonial uses

Turmeric is also used in various rituals, such as the turmeric ceremony or gaye holud, part of the Bengali wedding. It is widely used in all parts of India during wedding ceremony, particularly in North India and the Deccan Plateau.

It is also used in Pujas to make a form of the elephant God Ganesha. It is made by mixing turmeric with water and forming it into a cone-like shape.

During the south Indian festival Pongal, a whole turmeric plant with the root is placed as part of the ceremonial decorations. Also, fresh root is some times tied around the pot boiling the ceremonial rice.

In southern India, as a part of the marriage ritual, a piece of dried turmeric tied with string is sometimes used to replace the Thali necklace temporarily or permanently.

Modern Neopagans list it with the quality of fire, and it is used for power and purification rites.

In Goa and Dakshina Kannada (Karnataka state, India) turmeric plant leaf is used to prepare special sweet dishes, patoleo, by layering on the leaf — rice flour, and coconut-jaggery mixture, and then closing and steaming in a special copper steamer (goa).

Friedrich Ratzel in "The History of Mankind" reported in 1896 that in Micronesia the preparation of turmeric powder for embellishment of body, clothing and utensils had a highly ceremonial character.[10] He quotes an example of the roots being ground by four to six women in special public buildings and then allowed to stand in water. The following morning three young coconuts and three old soma nuts are offered by a priestess with prayer, after which the dye which has settled down in the water is collected, baked into cakes in coconut moulds, wrapped in banana leaves, and hung up in the huts till required for use.

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