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College of SCienCeS

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LaboratoriesLaboratories

ManualManual

College of Sciences’ Laboratories

Location /Lab # Functions Utilization/ Remarks

W12-039

Plant Biotechnology Protein Engineering General Biology Molecular Genetics Molecular Biology Microbial Processing & Biotechnol-

ogy Lab

Applied Biology Department

W12-035

Biology Pathology Phlebotomy Immunology Urinalysis Lab Blood Banking

Applied Biology Department College of Health Sciences (MLS

Department)

W12-031

Hematology Clinical Chemistry Biochemistry Biology Lab


Department)

W12-141

General Microbiology Medical Microbiology Diagnostic Microbiology Food Microbiology

Applied Biology Department College of Health Sciences (MLS, CND

Departments) Colleges of Medicine Colleges of Dentistry Colleges of Pharmacy

W12-240 General Chemistry Chemistry for Pharmacy

Chemistry Department

College of Pharmacy

W12-239

Analytical Chemistry Physical Chemistry Instrumental Analysis General Chemistry Inorganic Chemistry


W12-236 General Chemistry Chemistry for Pharmacy

Chemistry Department College of Pharmacy

W12-235 Organic Chemistry Identification of Organic Compounds


W12-232 Corrosion Analytical Chemistry Research Lab


W12-231 Polymers Chemistry Organic Chemistry Research Lab


W12-227

CHS Research Lab Molecular Genetics Immunology & Serology Blood Banking Urinalysis & Body Fluids Students Research Projects.


Department) College of Medicine

9999

W12-205 Biology and Human Anatomy Physiology Immunology and Serology

College of Health Sciences (MLS Department) Applied Biology Department

W12-213

Physics Research Physics for Medical Science Physics for Medical Imaging Electronics for Experimental Physics General Physics(1) Physics for Health Science Modern Physics Intermediate Physics

Applied Physics Department

W12-214 General Physics (1) Physics for Medical Science


W12-218 Physics for Health Science Physics for Medical Science General Physics (1)


W12-219

Physics (3) General Physics (2) Physics for Medical Science Physics for Health Science


M12-034 Analytical Chemistry Chemistry Department College of Pharmacy

M12-031 General Chemistry Chemistry Department

M12-029 Organic Chemistry Chemistry Department

M12-026 Biotechnology Research Lab Molecular Biology Lab Seeds Lab

Applied Biology Department Sharjah Academy for Scientific

Research

M12-025 General Chemistry Chemistry Department

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Applied Physics

Applied Physics’ Laboratories

Lab name Location Person in Charge Programs Served Courses Served

Physics (I) LaboratoryM12-106

W12-214

Sanoor Mannath Khadhija Alhou-

sani

College of Engineering Applied Physics

Physics (1) Lab

Physics (2) LaboratoryM12-103

W12-219

Mohammed Uth-man Saad Abdalla

College of Engineering Applied Physics

Physics (2) Lab

Physics (3) Laboratory M12-102 Mohammed Uth-man Applied Physics Physics (3) Lab

Electronics for Experi-mental Physics Lab W12-213 Taghrid Abdalla Applied Physics Electronics for

Physics

Modern Physics Lab M12-102 Mohammed Uth-man Applied Physics Modern

Physics

General Physics for Health Sciences Labora-tory

W12-218 Khadhija Alhou-sani

College of Health Sciences

General Phys-ics for Health Sciences

Physics for the Medical Sciences Laboratory

W12-213

M12-105

Taghrid Abdalla Sanoor MannatH

Medical Sci-ences

Physics for the Medical Sciences

Physics for Medical Imag-ing Lab M12-105 Sanoor Mannath Medical Sci-

ences

Physics for Medical Imag-ing

Thermal Properties Analy-sis Laboratory W12-213 C Dr. Bashir Sulei-

man RESEARCH LAB

Solar and Renewable Laboratory W12-024 Dr. Ala Jumaa RESEARCH LAB

Spectroscopy LaboratoryW12-213 B

Dr. Hussain Al Awadhi RESEARCH LAB

Acoustics Research Laboratory W12-213- A Dr. Hussein El

Mehdi RESEARCH LAB

Astronomy and Space Science Laboratory W12-024 Dr. Hamid Al

Naimiy RESEARCH LAB

101101

Physics (I) Laboratory

Introduction

This course is offered to first-year students enrolled in the College of Engineering and Col-lege of Sciences. The outline of this course is designed to teach students the application of

physics fundamentals in kinematics, energy and collision, and thermal properties of materials.

Equipment and Instruments Computers, Stand Rods and bases, measuring tools and devices ( ruler, tapes, meter stick,

micrometer …etc.), Electronic Timers, Force Tables, Air Tracks, Air Blowers, Frictionless Tracks, Trolleys, different Pasco sensors ( motion, pulleys,… etc), Pasco Interface, CASSEY Interface, Pro-jectile Launcher, Ballistic Pendulum, Springs, Simple Pendulum, Calorimeter, Heaters, Electronic Balances, Linear Thermal Expansion Apparatus, Hand Held Vacuum, Hot Plates, Jacks,.

Experiments • General Instructions, Lab Safety and Errors Calculations

• Data Analysis and Graphs, using Data Studio in Physics Labs

• Measuring Devices and Density

• Equilibrium of Forces at Body Joints

• Free Fall Motion

• Motion in 2-D (Projectile)

• Friction

• Uniformly Accelerated Motion

• Newton’s Second Law

• Conservation of Energy

• Conservation of Momentum

• Ballistic Pendulum

• Specific Heat Capacity

• Simple Harmonic Motion

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Physics (2) Laboratory

Introduction

This course is offered to second-year engineering students and science students. The course content is designed to complement the concepts taught in the theoretical component of

Physics 2, which focuses on electricity and magnetism. Students are expected to learn how to build basic circuits, perform simple circuitry measurements (for both DC and AC circuits), and examine Ohm’s law and resistance capacitance circuits. Students also measure magnetic fields and magnetic force as well as analyze AC circuits using digital oscilloscope.

Equipment and Instruments Computers, measuring meter ( ammeter, voltmeter, multimeter… etc), Rheostats, Resistance

Boxes, DC and AC Power Supplies, electronic elements ( capacitors, resistors, …..etc), Resistivity Apparatus , Potentiometers, Lamps, Switches, Meter Bridges, Stop Watches , electroplating appa-ratus, electric field mapping apparatus, High Current Power Supplies, CASSY software, CASSY sen-sors and Boxes( force, magnetic field,… etc ), Magnets, Set of Conducting Loops, Pasco Interface, Pasco sensors, Coils, Transformers, Oscilloscopes, Induction Coils.

Experiments • Circuit Construction and Measurements

• Electric Field Mapping

• DC Circuits

• Reistivity

• Ohm’s Law

• Potentiometer

• Bridge Measurement (Wheatstone Bridge and Capaci-tors Bridge)

• Resistance-Capacitance Circuit

• Kirchhoff’s Lows

• Electric Power Transfer

• CRO (Cathode Ray Oscilloscope)

• Magnetic Field

• Magnetic Force (Homogeneous Magnetic Field and Non-Homogeneous Magnetic Field)

• Electromagnetic Induction (Lens’z Low and Faraday’s Law)

103103

Physics (3) Laboratory

Introduction

This course is offered to first-year physics students . The course content is designed to complement the concepts taught in the theoretical component of Physics 3, which focuses

on Rotational motion, waves, simple harmonic motion, elasticity, fluid, heat and thermodynamics .

Equipment and Instruments Rotational and circular motion apparatus, thermal expansion apparatus, vibrating string and

standing waves, speed of sound apparatus, Young’s Modulus apparatus, simple pendulum and Hook’s law apparatus.

Experiments • Inertia of Different Objects

• Circular Motion

• Conation of Angular Momentum

• Young’s Modulus

• Hook’s Law and SHM

• Simple Pendulum

• Vibrating String and Standing Waves

• Buoyant Force

• Thermal Expansion

• Speed of Sound in Solids

• Speed of Sound in Air

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Electronics for Experimental Physics Lab

Introduction

The main objective of this laboratory is to introduce the concept of the electronic devices, including diodes, transistors, operational amplifier, logic gates, flip-flops, registers and coun-

ters, memories, switching and filtering to students. In addition to gaining experience with typical electronic circuits that are found in common applications, including the concept of design of their own projects.

Equipment and Instruments Power Supplies, measuring devices and tools ( ammeter, voltmeter,…, etc), Rheostats, elec-

tronic parts ( resistors, capacitors, …, etc ), Transformers, diodes of different types, Rectifier Diodes, transistors, integrated Circuits, Breadboards, Dual Trace Triggered Oscilloscope, Function Generator, Zener Diodes, 78xx Voltage Regulator, (3.0-6.2)V Charger, 9V Battery, 7-Segment Display, 7-Segment LC Display, 7447 Decoder, Different Kits, 741 Operational Amplifier, 555 timer, 2114 RAM Chip, TTL 740x Chip, 8086 Microprocessor, AM Radio Station, Operational Amplifier Radio, Potentiometers, Testing Probes, Soldering Iron, Circuit Board Holders, Transistor Tester.to

Experiments • General Instructions and Electronics Components

• Oscilloscope and its Uses in Electronics Lab

• Filters and RCL Circuits

• Diodes and Rectifier Circuits

• Zener Diode and Voltage Regulator

• Transistors

• Operational Amplifier (741)

• The 555 Timer

• Logic Gates

• Seven Segment Display and Decoders

• Semiconductor Memories

• Project

105105

Modern Physics Lab

Introduction

This course is offered to second-year students in the Applied Physics Department at the Col-lege of Sciences. It provides hands-on experience with experiments in modern physics that

are challenging at varying levels of expertise. The experiments are designed to enhance classroom learning of modern physics by performing some milestone physics experiments, and give students basic skill and technique in data acquisition and analysis, as well as in writing lab reports.

Equipment and Instruments Power Supplies, Measuring Tools and Devises (Ammeter, Voltmeter, …, etc), Transmitter,

Modulation Frequency, Leybold Light Receiver, Dual Trace Triggered Oscilloscope, Function Gen-erator, Optical Benches, Spectrometers, Light Sources, Laser Beams, Double Slit, Balmer Lamps, Power Supplies Unit for the Balmer Lamps, Optical Instruments (Mirrors, Lenses, Gratings, …, etc), Geiger – Muller ( G- M ) Tube, Radiation Sources, Set of Aluminum and Lead Plates, Count-Re-cording Device within Built High Tension, Digital Counter, Sealed Co60, and Sr90, Set of Aluminum and Lead Plates, (e/m) Apparatus, Lead Blocks, TEL-X-OMETER Apparatus, Pasco (h/e) Apparatus with Accessory Kit, Mercury Vapor Light Source, Zeeman Effect Optical System, Cadmium Lamp, Lummer-Gehrke-Plate, Frank-Hertz Tube with Operating Unit, Oven with Temperature Controller.

Experiments • Spectrum of Hydrogen Atom (Using Optical Bench and Spectrometer)

• Oscilloscope and its Uses in Modern Physics Lab

• Speed of Light Measurement

• Specific Charge of Electron (e/m)

• Frank-Hertz Experiment

• Radiation Detection and G-M Tube

• Absorption of Gamma and Betta

• Photoelectric Effect

• X-Ray Diffraction

• Zeeman Effect

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General Physics for Health Sciences Laboratory

Introduction

This course is designed specifically for students enrolled in the College of Health Sciences and for students of biotechnology in college of sciences, which includes departments such

as Medical Diagnostic Imaging, Nursing, Physiotherapy, Medical Laboratories, and Environment and Food Safety. The course includes experiments that are direct applications of concepts covered in the theoretical component of the course. It focuses on error calculation, graphing and analyzing data, measuring devices and density measurements, application of equilibrium of forces at body joints, application of torque (muscles and biomechanics), motion, fluid dynamics, thermal properties of materials, electrical properties of materials, optics and waves.

Equipment and Instruments Measuring Tools and Instruments (Caliper, Micrometer, Ammeter, Multimeter,…etc), Geo-

metrical Objects, Force Table Sets with Accessories, Torque Apparatus, Frictionless Tracks, CASSY Software and CASSY Sensors (Photo-Gates, G Ladder, Magnetic Field, ….etc), Linear Thermal Expansion Apparatus, Calorimeters, Heaters, Balances, Pasco Interface, Laboratory Jacks, Power Supplies AC-DC, Electronic Elements (Resistors, Capacitors,… etc), Geometrical Optical Sets, Laser Source, Stand Rods and Bases.

Experiments • Errors and Significant Figures, Graphs, and Mathematical Concepts

• Measuring Devices and Density Measurements (Density of Solid & Density of Fluid)

• Equilibrium of Forces at Body Joints

• Relationship of Skeletal Muscles to Bones

• Motion Along a Straight Line (Constant Velocity & Constant Acceleration)

• Magnetic Field of Current Carrying Conductors

• Thermal Properties of Materials (Thermal Expansion & Specific Heat Capacity of Human Teeth)

• Static Fluid and the Force of Buoy-ancy

• Simple DC Circuits

• Reflection, Refraction and Total In-ternal Reflection

107107

Physics for the Medical Sciences Laboratory

Introduction

This course is offered to students enrolled in the foundation year of the Medical Sciences Colleges, which include the College of Medicine and College of Dentistry. The course is

designed to introduce students to some of the medical applications of the concepts covered in Physics for Medical Sciences with emphasis on motion, forces, balance and torque (as applications to muscle dynamics).

Equipment and Instruments Measuring Tools and Instruments (Caliper, Micrometer, Ammeter , Multimeter,…etc), Geo-

metrical Objects, Force Table Sets with Accessories , Frictionless Tracks , CASSY Software and CASSY Sensors (Photo-Gates, G Ladder, Magnetic Field, ….etc), Linear Thermal Expansion Appara-tus, Calorimeters; Heaters, Balances, Pasco Interface, Laboratory Jacks, Power Supplies AC-DC, Electronic Elements (Resistors, Capacitors,… etc), Geometrical Optical Sets, Laser Source, Stand Rods and Bases, Electric Equivalent of Heat, Basic Radiation Measurement (Geiger Muller Tube, Radioactive Sources, Absorption Sheets of Different Materials and Thickness.

Experiments • Measuring Devices and Density

• Forces at Equilibrium

• Motion Along a Straight Line: Part A

• Motion Along a Straight Line: Part B

• Force of Buoyancy

• Heat Equivalence of Electrical Energy

• Linear Thermal Expansion

• Simple DC Circuits

• Magnetic Field Due to a Current Carrying Conductor

• Reflection, Refraction and Total In-ternal Reflection

• Radiation Detection and Absorption Measurements

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Physics for Medical Imaging Lab

Introduction

As indicated by its title, this course is offered to second and third-year students in the Medi-cal Imaging Department at the College of Health Sciences. The experiments are designed to

give students a strong foundation in various physics concepts and their applications, with emphasis on concepts that form the basis of imaging modalities such conventional X-rays, CT, PET, Magnetic Resonance Imaging, and Ultrasound.

Equipment and Instruments Power Supplies, Measuring Meters (Ammeter, Voltmeter…etc ), Electric Field Mapping Appa-

ratus, Electronic Elements ( Capacitors, Resistors,…., etc), Rheostats, Stand Rods and Bases, CASSY Software and Sensors ( Magnetic Fields, Force,…, etc ) , Solenoids, Circular Coils, Optical Benches, Spectrometers, Optics Parts ( Mirrors, Leses, Pench,…etc), Measuring Tools and Devices ( Rulers, Calipers,…, etc) , Laser Beams , Double Slit and Gratings, Leybold Electric Oven and Black Body Accessory, Balmer Lamps, Transmitter (Light-Emitting Diode - LED with Condenser), Modulation Frequency, Two-Channel Oscilloscopes, Geiger – Muller ( G- M ) Tube, Radiation Sources, (e/m) Apparatus, TEL-X-OMETER Apparatus.

Experiments • General Instructions (Purpose of the Lab, Lab Manual, Grading, Lab Preparation, Students’

Groups, Lab Procedure, Lab Reports)

• Electric Field (Uniform and Non-Uniform)

• Capacitance and Dielectric

• RC-Circuit

• Magnetic Field of Solenoid and Circular Coil (Uniform and non-Uniform)

• Oscilloscope and AC Signals

• Spectrum of Hydrogen Atom (Optical Bench & Spectrometer)

• Radiation Detection & Geiger-Muller Tube

• Absorption of Gamma and Betta

• Specific Charge of the Electron (e/m)

• Penetration and Attenuation of X-ray

• Ultrasound Waves

109109

Thermal Properties Analysis Laboratory

Introduction

Currently, this lab is capable of measuring at room temperature the thermal properties of solids that are directly related to heat conduction, namely, thermal conductivity, thermal

diffusivity and specific heat capacity. The transient plane source technique (TPS) known as Hot Disk instruments is used to measure the thermal transport properties of the solid materials. It comprises a completely novel test system designed specifically for measurement of low- to medium-conducting materials, including solids, powders, plastics and composites. It is specifically amenable to measure-ments of building essentials such as woods, fiber-boards, concrete, cement, and insulation materials.

Equipment and Instruments The lab has optional access to the Hot Disk, a chamber and circulating bath for measurements

from 90 °C down to -10 °C with software for automatic measurements and automatic temperature regulation.

Experiments • Developing Several Transient Techniques to Measure Thermal Properties of Solids

Tests and Services • Thermal Conduction Measurements of Solid Materials

• Linear Thermal Expansion Measurements

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Solar and Renewable Laboratory

Introduction

In general the Physics Research Laboratory (Solar and Renewable Laboratory) is equipped with advance systems which can be used for characterization of novel and new semiconductors

materials suitable for the fabrication of various hetero-junction systems used in different photo detectors applications.

Most of the work has been concentrated on II-VI,III-V binary, ternary, and quaternary com-pound semiconductors, specialty semiconductors used in opto- electric devices such as IR photo detectors and solar cells including InSb, InSbBi, GaAs, CdTe, GeSe, etc.

For the last four years, development of a software program has been carried out in coopera-tion with the Perkin Elmer Company (the manufacture of the Spectrophotometer). The program is currently used to incorporate the spectrophotometer technique to determine the energy gap and other optical constants of films prepared with various evaporation techniques under certain conditions.

In addition, the lab is equipped with advanced equipment for measuring the electric and opto-electric properties of thin films and electronic devices including: Resistivity, activation energy, dark and photo conductivity at various temperatures and I-V Characteristic, C-V measurement at dif-ferent frequencies.

The Hall Effect system is used to measure the minority or majority carrier concentration, Hall coefficient, life time, mobility, surface resistivity and type of semiconductor (n- or p-type) at room temperature and liquid nitrogen temperature.

Equipment and Instruments• Cryogenic System for Measuring the Dark and Photo Conductivity, I-V Characteristic Under

Various Illumination at Low Temperature (-196C) Under Vacuum

• WK 6440B LCR Meter Which are used for C-V Measurement for Junction (Homo and Het-ero) at Various Frequencies to Find the Depletion Layer and Built in Potential

• Kiethley 2425 Programmable for Measuring I-V Characteristic Junction (Homo and Hetero) in Dark and Illumination at Various Applied Voltage Forward and Reverse

• Various Kind of Advance Electrometers for Measuring Accurate Current, Voltage and Re-sistance

• UV-VIS Perkin Elmer Spectrophotometer for Measuring Optical Properties

• The Hall Effect System

Also the lab has basics tools needed to carry out the research such as:

• Chemical Growth Bath System

• High Accurate Meters

• Ultrasonic Bath

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• High Accurate Balance etc.

• Oven with Various Rate of Temperature Increments

Experiments • Complete Equipment Sets for Investigation of following Experiential Demonstrations:

• Investigation of Temperature Behavior of Different Solar Collectors

• Conversion of Radiating Energy Into Electric Energy- Characteristic Curves of a Solar Cell

• Generation of Gaseous Hydrogen

• AC Generator and DC Generator

• Series-Wound Motor With Drum Armature

• The Principles of Working of an Electric Motor

• Hall Effect- Determined the Band Gap of Semiconductor

Tests and ServicesThe lab is equipped with state-of–the-art systems; accordingly it provides the following services

and tests:

• Optical Properties of Thin and Thick Transparence Semiconductor Films

• Electric and Opto-Electric Properties of Thin Films

• I-V Characteristic and C-V Measurements for Junction (Homo and Hetero) at Various Fre-quencies

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Spectroscopy Laboratory

Introduction

The spectroscopy lab focuses on investigating intrinsic properties of novel and new semi-conductors. Photo-modulation reflectivity and transmission, and photoluminescence are

employed to determine the band-gap and other properties of semiconductors at temperatures down to 6K. Most of the work has been concentrated on II-VI binary, ternary, and quaternary compound semiconductors and specially diluted magnetic semiconductors in which some of the group II atoms are replaced by transition metal atoms like Mn, Fe, Co and V.

Equipment and Instruments • Liquid Nitrogen and Closed Cycle Helium Cryostats for Low Temperature Measurements

• Computer Controlled Spectrometers and Monochromators With Multiple Gratings Opti-mized for Different Spectral Regions

• Solid State Detectors That Cover the Spectral Region From UV to IR

• Different Lasers

• Lockin Amplifier for Low Level Signal Detection

Experiments • Reflectivity, Absorption and Transmission in the Range of 300-1700nm

• Photoluminescence using Blue, Green and Red Lasers

• Bandgap Measurement of Semiconductors at low Temperatures

Tests and Services • Reflectivity, Absorption and Trans-

mission of Solids and Liquids in the Range of 300-1700nm (Around Room Temperature)

• Reflectivity, Absorption and Trans-mission of Solids at Low Tempera-ture (Down to 6K)

113113

Acoustics Research Laboratory

Introduction

Research in the Acoustics Research Laboratory covers a number of areas on acoustics includ-ing assessment of environmental and occupational noise and ultrasonic characterization of

materials used in bio-medical and engineering applications. In the audible range, research is focused on assessing and monitoring community noise (roads, airports, schools, hospitals, factories, etc.) as well as acoustic mapping using advanced software packages (KADNA). In the ultrasonic range of the sound spectrum, research is focused on ultrasonic characterization on probing properties such bubble formation, concentration and growth in food systems at frequencies ranging from 50 kHz to 20 MHz.

Equipment and Instruments • Ultrasonic Flaw Detector

• High Voltage Pulser/Receiver Wide Range of Transducers Covering Fre-quencies From 24 kHz to 20 MHz

• NDT Multi-Layer Thickness Gauge (Ultrasound)

• Digital Noise Meter Nor140 Sound Analyzer

• KadnA Noise Mapping Software

• Spectran NF-5020

• Spectran HF-6080

Experiments The Laboratory houses advanced technical equipment and several noise monitoring digital me-

ters. For ultrasonic measurements, the lab is equipped with a high voltage puleser-reciever, which is capable of generating and receiving ultrasonic signals from the 20 kHz to 50 MHz. In Addition, the Lab is equipped with an ultrasonic flaw detector and an ultrasonic multi-layer thickness gauge meter that is capable of measuring thickness of layers down to a fraction of mm in thickness. For noise monitoring, the laboratory is equipped with a precession sound analyzer that is capable of noise source identification, assessing and monitoring environmental noise, measuring building acoustics and industrial acoustic hygiene, noise mapping, sound power, and speech intelligibility.

Tests and Services • NDT Flaw Detection (Ultrasound)

• NDT (Ultrasound) Characterization of Biomedical and Engineering Materials

• NDT Multi-Layer Thickness Measurements

• Noise Assessment

• Noise Prediction and Mapping

• EMC RF & EMF Spectrum-Analyzer

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Computational Capabilities

Introduction

The Applied Physics Department has built a Beowulf style Linux cluster to be used mainly for atomistic and band structure calculations. The core of the cluster is comprised of two

rack-mountable Tyan boards, each with two dual core Opteron processors effectively creating an 8-processor model cluster. An open heterogeneous architecture allows for the addition of any number of processors as soon as they are needed.

The cluster runs rocks 5.0 with MPI (message passing interface library). Examples of parallelized software available to users include NAMD, Gromacs and XMD for molecular dynamics, and wien2k for band structure calculations. A licensed proprietary copy of Maple 13.0 also runs on the cluster. Also included are several modern open source compilers including Fortran 90, C++, and C.

The cluster is available to all University researchers and their collaborators with access to the University of Sharjah LAN.

Equipment and Instruments • Parallel Computer Modules with Large Storage and Memory

• Software:

1. Parallelized Molecular Dynamics (XMD, NAMD, VMD, Gromacs, and Others

2. Wien2k: Band Structure Calculations

3. Fortran, C, C++ Compilers, Python, Scientific Python

4. Parallel Maple

5. Other Software Can Be Installed on Demand

Experiments • Molecular Dynamics Simulations

• Atomistic Simulations

• Other Modeling Experiments

Tests and Services • Provide Computing Time to Clients

• Provide Access to Powerful Num-ber Crunching Machine with Open Source Software

115115

Astronomy and Space Science Laboratory

Introduction

In the field of Astronomy, the Applied Physics staff is involved in several research projects including:

• Astronomy and Astrophysics (Variable Stars, X-Ray Binary Stars)

• Very Low Frequency Observations of the Lower Ionosphere at Site UAE as Part of Asia Sec-tor (Providing a basis for comparison to facilitate global extrapolations and draw conclusions. This research is being conducted in collaboration with Stanford University as part of the electromagnetic phenomenon studies of the Ionosphere and Magnetosphere)

• Babylonian Astronomy, Arab &Muslims Heritage Astronomy

• Maritime Astronomy/Astronomical Instrumentations Invented by the Muslims Scholars and used in Maritime

• Astronomical Applications in the Islamic Sharia’a (Praying Time Measurements, Problems of Crescent Visibility in Hijira Calendar…etc)

• Educational Astronomy and Astrophysics

The Astronomy and Space Sciences Research Team is in the process of purchasing a very low frequency remote sensing (in collaboration with Stanford University), which will be used for in-vestigating the lower ionosphere and magnetosphere to study environmental and weather patterns.

Equipment and Instruments • The Astronomy and Space Sciences Research Team has recently installed a very low frequency

(VLF) remote sensing receiver (in Collaboration with Stanford University), which will be used for investigating the lower Ionosphere and Magnetosphere to study environmental and weather patterns.

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Chemistry

Chemistry’s Laboratories

Lab Name Location Person in Charge Programs Served Courses Served

General Chemistry (I) Laboratory

W12-236

M12-025

KIfah Al-Taqaz Mohamed Al-

Farouq

Chemistry Dept. College of Engineering

General Chemistry (I) lab

General Chemistry (II) Laboratory W12-239 Hamid Idris

Chemistry Dept. Applied Physics Dept. College of Engineering

General Chemistry (II) Lab

General Chemistry Labo-ratory for Health Sciences Students

W12-240

M12-031

Fatima Ahmed Hamid Idris

Biotechnology Dept. College of Health

Sciences

General Chemistry Lab for HS

Analytical Chemistry Laboratory M12-034 Mohamed Al-

Farouq Chemistry Dept. Biotechnology Dept.

Analytical Chemistry Analytical Chemistry

for HS

Organic Chemistry Labo-ratory for health Sciences Students

W12-235 Nemat Dak El-Bab Biotechnology Dept. College of Health

Sciences

Organic Chemistry for HS

Organic Chemistry (I) Laboratory W12-235 Nemat Dak El-Bab Chemistry Dept. Organic Chemistry

(I) Lab

Organic Chemistry (II) Laboratory W12-235 Nemat Dak El-Bab Chemistry Dept. Organic Chemistry

(II) Lab

Identification of Organic Compounds W12-235 Nemat Dak El-Bab Chemistry Dept. Identification of Or-

ganic Compounds

Instrumental Analysis Laboratory

W12-239

W12-231D Kifah Al-Taqaz Chemistry Dept. Instrumental Analysis

Inorganic Chemistry Laboratory W12-239 Nemat Dak El-Bab Chemistry Dept. Inorganic Chemistry

Lab

General Chemistry Laboratory for Medical Sciences Students

M12-029

M12-031

Hajar Ibrahim Hamid Idris

College of Medicine College of Dentistry


Physical Chemistry (I) Laboratory W12-239 Kifah Al Taqaz Chemistry Dept. Physical Chemistry (I)

Laboratory

Physical Chemistry (II) Laboratory W12-239 Kifah Al Taqaz Chemistry Dept. Physical Chemistry (II)

Laboratory

Corrosion and Analytical Chemistry Research Lab W12-232

Dr. Ayssar Nahle Dr. Ahmed Al-

Mahdai Chemistry Dept. Chemistry Senior

Project

Polymers and Organic Chemistry Research Lab W12-231

Dr. Mahmoud Mohsin Prof. Ideisan Abu-

Abdoun Dr. Mohamed

El-Naggar

Chemistry Dept. Chemistry Senior Project

117117

General Chemistry (I) Laboratory

Introduction

This lab is designed to teach students of the college of engineering and college of sciences the basic qualitative and quantitative aspects of general chemistry.

Equipment and Instruments• Analytical Balances

• Ovens

• Heating and Cooling Systems

• Centrifuges

Experiments • The Scientific Methods: Measurements and Significant Figures

• Determination of the Density of Liquids and Solids

• Melting Point and Boiling Point

• Separation of a Mixture

• Composition of Hydrated Salt

• Heat of Reaction

• Determination of the Gas Constant R

• Molar Mass of Volatile Liquid

• Qualitative Analysis of Anions

• Qualitative Analysis of Cations

• Paper Chromatography

• Molar Mass Determination from Freezing Point Depression

Tests and Services • Determining the Density of

Solids & Liquids

• Determining the Melting & Boiling Point

• Qualitative Determination of Anions and Cations

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General Chemistry (II) Laboratory

Introduction

This laboratory course is designed to teach various practical methods such as spectrophoto-metric methods, pH measurements, titration methods, thermodynamics, and kinetics.

Equipment and Instruments • Analytical Balances

• Ovens


• pH Meters

• Different Ions Selective Electrodes

• Spectrophotometer

• Conductometers

Experiments • Determination of Equilibrium Con-

stant

• Quantitative Analysis: Acid–Base Titration

• Hydrolysis

• Chloride Ion Titration

• Solubility Product of Lead Iodide

• Complex and Double Salts of Copper (II) Ion

• Determination of the Order of Reaction

• The Effect of Temperature on Reaction Rate

• Functional Groups

• Oxidation States of Vanadium

• Quantitative Yield of a Redox Reaction

• Synthesis of Aspirin

Tests and Services • Titration Methods

119119

General Chemistry Laboratory For Health Sciences Students

Introduction

This lab is designed to teach general chemistry for the college of health sciences. The main purpose of this lab is to provide students with a strong practical basis in the principles and

practices of chemistry to conduct various qualitative and quantitative experiments.

Equipment and Instruments • Analytical Balances

• Ovens

• Heating Cabinets

• Centrifuges


• pH Meters

• Hot Plates with Stirrers

Experiments • The Scientific Methods: Measurements and Significant Figures

• Determination of the Density of Liquids and Solids

• Melting Point and Boiling Point

• Determination of the Gas Constant R

• Quantitative Yield of a Redox Reaction

• Composition of Hydrated Salt

• Qualitative Analysis of Anions

• Qualitative Analysis of Cations

• Quantitative Analysis: Acid-Base Titration

• Hydrolysis


• Molar Mass from Freezing Point Depression

Tests and Services • Density of Liquids and Solids Determination

• Melting and Boiling Point Determination

• Qualitative Determination of Anions and Cations

• Acid-Base Titration

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Analytical Chemistry Laboratory

Introduction

This lab introduces students to the concepts of the basic practical analytical chemistry and develops advanced laboratory skills in analytical chemistry. Students learn the standard

methods of volumetric and gravimetric analysis.

Equipment and Instruments• pH Meters

• Different Selective Electrodes

• Ovens

• Analytical Balances

• Conductometers

• Potentiometers

• Digital Voltmeters

• Muffle Furnace

Experiments • Precision of Measurements

• pH Instrumentation and Measurements

• Buffers

• pH Titration

• Standardization of NaOH and Determination of an Unknown Acid

• Determination of a Carbonate Unknown

• Complexometric Titration of Zinc

• Titration of Chloride Unknown Solution by Volhard Method

• Analysis of Solid Specimen of Iron

• Gravimetric Determination of Nickel

• Gravimetric Determination of Iron as Iron (III) Oxide

• Gravimetric Determination of Calcium as CaC2O4.H2O

Tests and Services • Preparation of Standard Solutions

• Quantitative and Qualitative Analysis of Ions and Metals

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Organic Chemistry Laboratory for Health Sciences Students

Introduction

This laboratory course is designed to teach practical organic chemistry to Health Sciences students. It covers various topics in the practical methods of separation, purification, iden-

tification and synthesis of organic compounds.

Equipment and Instruments• Rotatory Evaporator

• Hot-Plates with Magnetic Stirrers

• Distillation System

• Ovens


• Melting Point Apparatus

• Heating Cabinets


Experiments • Purification by Recrystallization

• Synthesis of Polymers

• Isolation of Caffeine from Tea Leaves

• Nitration of Methyl Benzoate


• Oxidation of Alcohols

• Identification of Alcohols and Phe-nols

• Identification of Aldehydes and Ke-tones

• Properties of Carboxylic Acids and Esters

• Preparation and Properties of Soap

• Properties of Amines and Amides

• Structure in Organic Compounds

Tests and Services • Separation, Purification and Identification of Organic Compounds

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Organic Chemistry (I) Laboratory

Introduction

This laboratory course is designed to teach Practical Organic Chemistry for chemistry stu-dents. This course covers various topics in the practical methods of separation, purification,

extraction, and synthesis of organic compounds as well as their identification.


• Ovens

• Fractional Distillation



Experiments • Measuring Melting Points of Com-

pounds and Mixtures

• Crystallization

• Extraction of Caffeine from Tea with Mythylene Chloride

• Separation of a Mixture of Cyclohexane and Toluene by Fractional Distillation

• Nitration of Methyl Benzoate

• Oxidation of Alcohols – Preparation of Cyclohexanone

• Preparation of an Alkene from an Alcohol

• Thin Layer Chromatography – Analysis of Analgesic Drugs

• Steam Distillation–Isolation of an Essential Oil

• Oxidation of Alcohols – Preparation of Adipic Acid

• Identification of an Unknown – Carbonyl Group

• Molecular Modeling

Tests and Services • Measuring Melting Points of Organic Compounds and Mixtures

• Thin Layer Chromatography – Analysis of Analgesic Drugs

• Isolation of an Essential Oil

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Organic Chemistry (II) Laboratory

Introduction

This laboratory course is designed to teach advanced practical organic chemistry to chemistry students. This course covers various syntheses of organic compounds and basic spectro-

scopic techniques for the identification of organic compounds.

Equipment and Instruments• Distillation Systems



• Spectroscopic Instruments

Experiments • Synthesis of Benzilic Acid from

Benzil

• Auto-Oxidation and Reduction of Benzaldehyde (Cannizzaro Reaction)

• Benzil from Benzoin

• Diels-Alder Reaction

• Grignard Synthesis of Triphenyl Methanol

• Preparation of Aniline from Nitrobenzene

• Synthesis of Sulfanilamide

• Dibenzalacetone by Aldol Condensation

• Borohydride Reaction of Benzil

• Synthesis of Stilbene from Benzoin

• Oleic Acid from Olive Oil

• Derivatives of 1,2-Diphenylethane – a Multi-Steps Synthesis

• IR and NMR Spectroscopy

Tests and Services • Synthesis of Organic Compounds

• Identification of Organic Compounds by Spectroscopic Techniques

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Identification of Organic Compounds

Introduction

This course covers various physical properties, purity determination, molecular formulas, and classification by solubility, spectrometric methods, functional groups by chemical tests,

preparation of derivatives and separation of mixtures are covered.


• Distillation Systems



• Spectroscopic Instruments

Experiments • Identification of Unknowns Preliminary Examination, Physical Properties, and Elemental Anal-

ysis

• Classification of Organic Compounds by Solubility

• Spectrometric Methods

• Chemical Tests for Functional Groups

• The Preparation of Solid Derivatives

• Separation of Mixtures

• Structural Problems - Solution Methods and Exercises

Tests and Services • Synthesis of Organic Compounds and their Derivatives

• Identification of Organic Compounds by Spectroscopic Techniques

• Separation of Organic Mixtures

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Instrumental Analysis Laboratory

Introduction

This course is designed for chemistry majors. Topics are chosen to give a range of experi-ments in order to provide students with a solid background in practical instrumental analy-

sis, which includes spectroscopic, electroanalytical and chromatographic methods.

Equipment and Instruments• UV-Visible Spectrophotometer

• HPLC

• Atomic Absorption Spectroscopy

• Flame Photometry

• ICP-OES

• GC-FID

• GC-MS

• Cyclic Voltammetry

Experiments • Visible Spectroscopy: “Determination of Cobalt and Chromium Ions in the Presence of Each

Other

• Ultraviolet Spectroscopy: “Quantitative Analysis of Aspirin Tablets”

• Inductively Coupled Plasma Spectroscopy: “Determination of Metal Ions in a Mixture and Study of the Interferences Effects”

• Flame Atomic Absorption: “Determination of Iron and Copper in An Unknown Sample”

• Flame Emission Spectroscopy: “Determination of Sodium in an Unknown Sample”

• Atomic Absorption Spectroscopy: “Determination of Copper in Unknown Sample”

• Electrochemistry: “Cyclic Voltammetry of Platinum in Ferro- / Ferri-Cyanide Solution”

• Electrochemistry: “Anodic Stripping Voltammetry for Simultaneous Determination of Pb2+, Cd2+, and Zn2+ Ions in Solution”

• Gas-Liquid Chromatography (GC): “Analysis of Hydrocarbon Mixture”

• Gas-Liquid Chromatography (GC): “Analysis of Alcohols Mixture”

• High-Performance Liquid Chromatography (HPLC): “Determination of Caffeine in Beverages”

Tests and Services • Metals Determination

• Characterization and Identification of Chemical Compounds Mixtures

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Inorganic Chemistry Laboratory

Introduction

This course includes experiments that aim to develop the student’s laboratory skills and demonstrate the majority of important techniques in inorganic chemistry. Experiments are

based upon synthesis and physical measurements of coordination and organometallic compounds.


• UV-Visible Spectrophotometer


• Gouy Balance

Experiments • Preparation of [Hexaamminenickel

(II)] Chloride, [Ni(NH3)6]Cl2

• Preparation of Hexaaminecobalt (III) Chloride, [Co(NH3)6]Cl3

• Preparation of Carbonato-tetraaminecobalt (III) Nitrate, [Co(NH3)4CO3]NO3

• Preparation of [Chloropentaamminecobalt(III)] Chloride by two Methods, [Co(NH3)5Cl]Cl2

• Nitritopentaamminecobalt(III) Chloride & Nitropentaammine-cobalt (III) Chloride [Co(NH3) 5ONO] Cl2 & [Co(NH3)5NO2]Cl2

• Synthesis of Cis-Potassium Diaquabis (Oxalato) Chromate (III) Dihydrate: Cis-K [Cr(C2O4) 2(H2O)2]Δ2H2O

• Magnetic Susceptibility, Magnetism

• UV-Vis Spectra for Transition-Metal Complexes

• Preparation of Cis and Trans Bis (Glycinato)Copper(II).Hydrate

• Preparation of Metal Acetylacetonato Compounds

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Introduction

This laboratory course is designed to teach the practical general basic and organic chemistry to medical students. This course covers various topics in the practical methods of purifica-

tion, identification, chromatography, and synthesis of organic compounds. It also covers qualitative and quantitative aspects of the General Chemistry Laboratory.

Equipment and Instruments• Heating and Cooling Systems


• Ovens

• Centrifuges

• Suction Filtration


Experiments • Qualitative Analysis of Anions and

Cations


• Purification by Recrystallization

• Isolation of Caffeine from Tea Leaves


• Oxidation of Alcohols: Preparation of Benzoic Acid

• Identification of Alcohols

• Identification of Aldehydes and Ketones

• Quantitative Analysis: Acid-Base Titration

Tests and Services • Qualitative Analysis of Anions and Cations

• Acid-Base Titration

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Physical Chemistry (I) Laboratory

Introduction

This course coves the necessary laboratory techniques for physical measurements, error analysis and statistics through conducting experiments on gases, calorimetry, equilibrium

and phase changes.


• Barometers

• Water Bath

• Shakers

• Bomb Calorimeter

• Refractometer

• Viscometer

• Manometer

• Temperature Sensor

• Pressure Sensor

• Gas Gauge

Experiments • Fundamentals: Error Analysis and Data Col-

lection

• Heat Capacity Ratios for Gases

• The Second Virial Coefficient

• Heat of Formation of Naphthalene

• Determination of Resonance Energy of Ben-zene

• Mutual Solubility of Liquids in Binary System

• The Vapor Pressure and Heat of Vaporization of Liquids

• Solid-Liquid Equilibrium in a Binary System

• Liquid-Vapor Phase Diagram: Calibration using Refractive Indices

• Liquid-Vapor Phase Diagram: Constructing the Phase Diagram

• Molecular Weight and Monomer Linkage Properties of Poly (Vinyl Alcohol)

• Phase Diagram of Tertiary System

• Entropy of Mixing

Tests and Services • Thermal Analysis

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Physical Chemistry (II) Laboratory

Introduction

Physical Chemistry (II) Laboratory introduces students to advanced laboratory skills and tech-niques to study chemical reactions kinetics and mechanisms.


• Ovens

• Oxygen Gas Sensors

• Gas Sensors

• Conductometers

• Viscometer

• Manometer

• Pressure Sensor

• Temperature Sensor

Experiments • Measurements, Precision and the Treatment of Experimental Data in the Physical Chemistry

Laboratory

• Surface Tension. Properties of Liquids

• Study the Effect of Temperature on the Surface Tension of Water

• Viscosity of Liquids: Low Viscosities. The Flow Times of each of (Methanol/Water) and (Toluene/P-Xylene) Solutions at 25oC

• The Flow Times of P-Xylene at Different Temperatures

• Viscosity of Liquids: High Viscosities. Using Falling Ball Viscometer: Measuring the Falling Times of Glycerol vs. Temperature

• Determination of Collision Diameters from Gas Viscosities

• Enzyme Action: Testing Catalase Activity in Different Conditions; Enzymatic Hydrolysis of Hydrogen Peroxide by Peroxidase

• Enzymatic Hydrolysis of Hydrogen Peroxide by Peroxidase. Testing the Effect of Temperature

• Kinetics and Mechanism of Heterogeneous Reaction: Oxidation of Magnesium by Hydro-chloric

Tests and Services • Gas and Liquid Viscosity Determination

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Corrosion and Analytical Chemistry Research Laboratory

Introduction Several types of analytical chemistry research may be carried out in this lab such as:

• Industrial Analysis

• Water Analysis and Treatment

• Corrosion and Protection of Metals

• Electrochemistry

Equipment and Instruments • Electrochemical Work Station

• Impedance Spectrometer

• Ultrasonic Water Bath

• Various Types of Glassware

• Analytical Balances, Conductometers, pH Meters, etc.

Tests and Services Offering Consultancy in:

• Corrosion Protection & Control

• Industrial Processing

• Environmental Analysis

• Waste Products Analysis

The Following may be Analyzed:

• pH of Soil

• Water Soluble Sulfate in Ground Water and Soil Extracted

• Water Soluble of Chloride in Ground Water and Soil Extracted

• Acid Soluble Chloride in Soil

• Acid Soluble of Sulfate in Soil

• Water Soluble Chloride in Aggregate

• Specific Gravity and Eater Absorption in Cores Aggregate

• Specific Gravity and Water Absorption of Fine Aggregate

• Acid Soluble Chloride in Aggregate

• Acid Soluble Sulfate in Aggregate

• Water Soluble Sulfate in Aggregate

• Acid Soluble Materials in Fine Aggregate

• Chloride Content of Cement

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• pH Value in Water

• Chloride Content in Water

• Sulfate Content in Water

• Total Dissolved Solids in Water

• pH Value of Water and Waste Water

• Conductivity of Water and Waste Water

• Total Dissolved Dolids in Water and Waste Water

• Suspended Solids in Water and Waste Water

• Total Solids in Water and Waste Water

• Acidity in Water and Waste Water

• Alkalinity in Water and Waste Water

• Nitrate – Nitrogen in Water and Waste Water

• Nitrite – Nitrogen in Water and Waste Water

• Sulfate in Water and Waste Water

• Chloride in Water and Waste Water

• Volatile Solids in Water and Waste Water

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Polymers and Organic Chemistry Research Laboratory

Introduction Several types of Polymers and Organic chemistry research may be carried out in this lab such as:

• Extraction of Various Organic Natural Products

• Synthesis of Different Organic Compounds

• Synthesis of Different Water Soluble Polymers and Polymer Blend

• Identification of Major Organic Functional Groups

• Catalysis

• Polymer Characterization and Analysis

Equipment and Instruments • High Performance Liquid Chro-

matography (HPLC)

• UV/Visible Spectrometer

• Various Types of Glassware

• Rotary Evaporator

• Refractometers

• Shakers

• Heating Mantles

• Magnetic Stirrer Hotplates

• General Purpose Water Bath

• Vacuum Oven


• Viscometer

Tests and Services • Offering Consultancy on Poly-

mer Synthesis, Characterization and Analysis

• Separation and Identification of Natural Products

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Biotechnology

Biotechnology’s Laboratories

Lab Name Location Person in Charge Programs Served Courses Served

General Biology Lab W12-039 Zahra Yassin Biotechnology Department General (Basic Course)

General Microbiol-ogy Lab M12-132 Aisha Echtibi Biotechnology

Department

Tissue Culture & Hy-bridoma Technology Molecular Genetics Molecular Biology Plant Biotechnology Microbial Processing &

Biotechnology Environmental Biotech-

nology

Immunology & Serol-ogy Lab W12-205 Aisha Echtibi Biotechnology

Department Immunology & Serol-

ogy Lab

Tissue Culture & Hy-bridoma Technology M12-132 Mona Ibrahim Biotechnology

Department Tissue Culture & Hy-

bridoma Technology

Molecular Genetics Lab W12-039 Mona Ibrahim Biotechnology

Department Molecular Biology

Molecular Biology Lab W12-039 Zahra Yassin Biotechnology

Department

Molecular Biology Student Research

Project

General Biochemistry Lab W12-031 Aisha Echtibi Biotechnology

Department

General Biochemistry Student Research

Project

Protein Biochemistry & Engineering Lab W12-031 Zahra Yassin Biotechnology

Department

Protein Biochemistry & Engineering Lab Student Research

Project

Plant Biotechnology Lab W12-039 Mona Ibrahim Biotechnology

Department

Plant Biotechnology Student Research

Project

Microbial Processing & Biotechnology Lab W12-141 Zahra Yassin Biotechnology

Department

Microbial Processing & Biotechnology Student Research

Project

Environmental Bio-technology Lab M12-132 Mona Ibrahim Biotechnology

Department

Environmental Biotech-nology Student Research

Project

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General Biology Lab

Introduction

Experiments involve the use of the microscope, details parts and functions. Furthermore, the chemical and physical characteristics of macromolecules will be illustrated, as well as,

the structure and composition of plant and animal cells. A section of this lab will also be devoted to respiration, cell division, and genetics. Finally the last section will mainly focus on the study of animal tissues in addition to the human anatomy.

Experiments • Microscopy

• Molecules of Living Cells

• Enzyme Action

• Respiration and Photosynthesis

• Cell Division

• Human Genetics

• Extraction of DNA

• Animal Tissues & Anatomy

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General Microbiology Lab

Introduction

The laboratory is designed to provide practical and hands-on experience of a variety of mi-crobiological techniques. Laboratory work includes microscopy, staining, aseptic and sterile

transfer techniques, assessment of morphological and biochemical properties of microorganisms and microbial identification.

Experiments • Lab Safety

• Microcopy and Simple Staining

• Culturing and Isolation Techniques

• Selective and Differential Staining of Microbes

• Microbial Morphology

• Selective and Differential Media & Demonstration of Bacterial Culture Characteristics

• Control of Microorganisms by Physical & Chemical Factors

• Bacterial Growth Curve

• Microbial Control

• Biochemical Testing

• Identification of Unknown

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Immunology and Serelogy Lab

Introduction

This course is designed to prepare the student to function at the career-entry skill level in the immunology laboratory. This course presents basic principles of immunological and

serological techniques. The experiments are designed to study the immune response, principles of antigen-antibody reactions, and the principles of serological procedures. The lab includes other ar-eas of study concerning the fundamentals of immunity and the immune response such as antibody structure and interactions, the complement system, hypersensitivity reactions and disorders of the immune response.

Experiments • Concept of Different Types of Dilutions in Immunology and their Importance in Clinical

Laboratory.

• Observation of Phagocytosis by Preparing Wet and Dry Mount

• Direct Latex Agglutination (Antistreptolysin-O-Latex test, Febrile Antigen Test, Anti-Deoxy-ribonucleic Acid, Rheumatoid Factor Latex Test and C Reactive Protein Test )

• Indirect Agglutination (Stapylococcus Aureus Rapid Test )

• Using Reagent Strips for Urinalysis

• Latex test for the Identification of Different Groups of Streptococci using Chemical and Enzymatic Extraction Methods

• ELISA (Enzyme Linked Immunosorbant Assay)

• Using Oxidase Reagent to Identify Pathogenic Bacteria such as Neisseria

• Allergy Screening Test

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Tissue Culture and Hybridoma Technology

Introduction

The course is designed to study the basic principles of animal tissue culture. The course will also introduce the student to basic hybridoma technology concepts and monoclonal anti-

bodies production techniques.

Experiments • Lab Safety & Aseptic Techniques

• Introduction to Cell Culture: Me-dia & Cell Types

• Subculturing, Cell Quantification & Viability Studies

• Cell Differentiation

• Detection & Control: Mycoplasma, Bacteria & Fungus

• Cryopreservation

• Isolation of Primary Cells

• Culturing Primary Cells

• Growing Hybridomas

• Isolation of Monoclonal Antibody

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Moelcular Genetics LAB

Introduction

This course is an introduction to many areas of modern genetics and emphasizes the prin-ciples of genetics in each of 4 main areas, transmission, cytogenetics, quantitative and

molecular genetics. The lab will begin by introducing students to the concepts and the statistical laws commonly used in genetics. The key section of this lab will focus on studying how genes are transmitted between generations and what are the laws governing their transmission; these topics will mainly be investigated in Maize (Corn) and Drosophila as the model organism. Some sections of this lab will arm students with a good experience in karyotyping. Moreover, bacterial mutagenesis will be covered in this lab, and in the last section, students will be asked to extract and characterize different types of genetic material.

Experiments • Introduction, Probability & Statistics

• Meiosis & Mitosis

• Independent Assortment and Gene Interaction in Maize

• Drosophila Familiarization, Mendelian Inheritance in Drosophila, Sex-Linked Inheritance in Drosophila

• Crossing over in Drosophila

• Salivary Gland Chromosomes of Drosophila

• Mutations in Bacteria

• Karyotyping

• Isolation/Characterization of Chromosomal-Plasmid DNA, Agarose Gel Electrophoresis

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Moelcular Biology LAB

Introduction

This course is designed to enhance the preparation of students for careers in research, bio-technology and science education. The laboratory experience will introduce students to the

field of molecular biology where they will learn all the basic methodologies and instrumentation commonly used in biotechnology and molecular biology laboratories. Students will learn bacterial transformation, plasmid DNA purification, restriction digestion and ligation along with gel electro-phoresis, polymerase chain reaction (PCR), cloning DNA fragments, and selection of recombinants. This course will give students who plan career in research or biotechnology practical experience that mimics the realities of the laboratory setting.

Experiments • DNA isolation, Concentration Calcula-

tions, Purity

• Restriction Enzyme Digestion and Liga-tion

• DNA Gel Electrophoresis

• DNA Gel Extraction

• DNA Basics: Bacterial Transformation

• Selection of Recombinants: Antibiotic Resistance and Blue White Screening

• Designing Primers for PCR Amplification

• Reverse Transcription PCR

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General Biochemistry LAB

Introduction

The aim of this Laboratory course is to provide an introduction to general laboratory tech-niques routinely used in Biochemistry with emphasis on the qualitative as well as quantitative

aspects of the biochemical experiments. The course deals with principles and practices of Biochem-istry, chromatography for the investigation of metabolites, proteins, polysaccharides, application of enzymes for quantitative determinations by spectroscopic methods, and introductory experiments about kinetics of enzyme action.

Experiments • Laboratory Calculations and Instrumentation

• pH Measurement and Buffer Preparation

• Titration of unknown Amino Acid

• Chromatographic Analysis of Amino Acids

• Spectrophotometers and Protein Determination

• Enzyme Kinetics

• Protein Purification and Analysis on SDS-PAGE

• Qualitative Test for Carbohydrates

• Glycolysis and Fermentation in Yeast

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Protein Biochemistry & Engineering LAB

Introduction

This course covers basic biochemical principles of proteins along with a comprehensive sur-vey of products currently available or under development by the protein biotechnology

industry.

Experiments• Protein Purification

• Salting Out

• Dialysis

• Gel Filtration

• Expression and Characterization of a Recombinant Enzyme in E.Coli.

• SDS Gel Electrophoresis, Molecular Weight Measurements; Protein Gel Staining (including the whole cell homogenates); Gel Drying

• Western Blotting Concepts; SDS PAGE and Transfer

• Western Blot Analysis

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Plant Biotechnology LAB

Introduction

This course will introduce the students to the subject of plant biotechnology. The biotech-nological methods involved in the production of transgenic plants will be described – plant

tissue culture and plant genetic engineering – in plant models such as Arabidopsis thaliana. The ap-plication of these techniques to the improvement of agricultural important species will be discussed, as well as the social, ethical and ecological issues involved in the use of these engineered organisms.

Experiments• Aseptic Techniques and Introduction to Plant Tissue Culture

• Nutrient Media Preparation for Plant Tissue Culture

• Seed Sterilization - Seed Germination

• Plant Response to Hormones

• Plant Regeneration by Organogenesis

• Leaf Disc Transformation by Agrobac-terium

• Detection of Agrobacterium Tumefa-ciens in Soil

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Microbial Processing & Biotechnology LAB

Introduction

The course focuses on the vast array of applications of microorganisms. It is aimed to high-light the potential of wild or genetically modified microorganisms as cell factories for the

production of goods and services. It introduces the student to the new era of biotechnology & familiarizes the student with the several areas of industrial application of microorganisms: medi-cal and pharmaceutical, protein production (enzymes, yeast, and single cell protein), production of biochemicals (primary/secondary metabolites), food and beverage technology, agriculture and environmental technologies and alternative energies (bioenergy).

Experiments• Isolation of Streptomycetes from Soil Samples and Testing their Potential to Produce Anti-

biotics

• Effect of Different Sweeteners on the Fermentation of Yeast

• Pickle Fermentation

• Using Chymosin to Make Cheese

• Fermentation

• Growth Curve of Saccharomyces Cerevisiae

Field Visits• Al-Rawabi Dairy Farm, Dubai

• Central Food Control and Consultancy Laboratory, Sharjah Municiplaity

• Julphar Pharmaceutical Industries, RAK

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Environmental Biotechnology Lab

Introduction

This course gives an overview of how biotechnical tools can be applied in waste handling, pre-vention and removal. The specific application of metabolic capability and molecular biology

of microorganisms to degrade environmental contaminants and remediate environmental media will be emphasized. The course format is a combination of lecture, laboratory, visits, projects and oral presentations. The labs will be field-based hands-on experiences designed with a goal to acquaint the students with some of the concepts which are critical to understanding issues in environmental management. The course will focus on topics that range between environmental monitoring, sew-age treatment, bioremediation and clean technology to renewable energy and biofules. One or two field trips that will highlight the application of biotechnology to reverse environmental problems will also be included.

Experiments• Introduction to Contaminants in UAE

• Identifying Bacterial Consortium in Soil Contaminated with Crude Oil

• Examination of Treated Sewage Water: Coliform MPN Test Experiment

• Composting, Building Your Own Bioreactor

• The Ability of Bacteria to Degrade Diesel Fuel

• Using Alkane Hydroxylase B (alkB) Gene as a Biomarker for Hydrocarbon Degredation

• Preparation of Biodiesel from Cooking Oil

Field Visits• Environmental Engineer Lab at CL/UOS.

• Waste Water Treatment Plant, Sharjah Municiplaity

• Compost plant, Sharjah Municiplaity

• Land Fills, Bee’ha

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