Conference on Diabetes• Types of diabetes mellitus

type 1 -- insulin dependent type 2 – insulin independent

• Experiment with type 1 genetic or environmental w/immuno-supp

• Assay developmentradioimmunoassay – insulin concentrationshistologic evaluation with Bata cell detectionblood glucose measurementblood antibodies measurement

ISLETS Of LANGERHANS in pancreas

Acinar cells

islet

ISLETS OF LANGERHANS stained with aldehyde-fuchsin, which selectively stains secretory granules of insulin-secreting Beta cells

kidney

Blood sugar concentrations

• Normal range 80 – 120 mg/dl• Diabetic range >120 mg/dl

ROLES AND SPECIFIC ACTIONS OF ANTIOBODIES IN IMMUNITY

COMPLEMENT - MEDIATED LYSISOPSONIZATION - PROMOTE PHAGOCYTOSISTOXIN NEUTRALIZATION

PREVENTION OF MICROBIAL BINDING TO MUCOSAL SURFACE

VIRUS NEUTRALIZATION - INTERFERES WITH CELL PENETRATION

EFFECTOR T CELLS•DELAYED - TYPE HYPERSENSITIVITY T CELLS

CYTOTOXIC CELLS DESTROYS TRANSPLANTED ORGANS

Measure antibodies in blood

radioimmunoassay for insulin blood concentrations