crispr agbio san diego april 2017 agenda

Overcome Productivity Challenges, Increase Yield & Pioneer Sustainable Agricultural Practices by Optimizing the Application of CRISPR

Program Partner:

Tel: +1 212 537 5898 | Email: [email protected]

Researched & Developed By:www.crispr-agbio.com

CRISPR Precision Gene Editing

Greg Gocal, CibusJin-Soo Kim, Seoul National University Tom Greene, DuPont Pioneer

Neil Hoffman, United States Department of Agriculture (USDA) Kan Wang, Iowa State University Mark Cigan, Genus

April 18-20 2017 | San Diego, US

Book now to save up to $800

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Learn how leading academics at Seoul National University are optimizing the specificity of Cas9 and Cpf1 to reduce off-targets in plants and animals

Key Case Studies:

Discover how scientists at DuPont Pioneer and the Chinese Academy of Agricultural Sciences are optimizing CRISPR/Cas9 delivery through agrobacterium and biolistic delivery methodologies

Hear about the latest regulatory guidelines from the USDA and EPA for utilizing CRISPR gene editing for commercial product development within agriculture

Understand how DuPont Pioneer have developed and are progressing their CRISPR-edited Waxy Corn

Discover how Genus are advancing CRISPR gene editing to develop disease resistant livestock

CRISPR AgBio Precision Genome Editing Congress April 18-20 2017, San Diego, US

Tel: +1 212 537 5898 Email: [email protected]

www.crispr-agbio.com CRISPR Precision Gene Editing

Get to the forefront of CRISPR technology advancements for optimized precision gene editing in plants, crops and livestock

Key Takeaways:

Advance your application of CRISPR gene editing in functional genomics for enhanced trait discovery in plants & crops

Discover how to optimize your delivery methodologies of CRISPR/Cas9

Discover Essential Technology Advancements & CRISPR Applications in Agriculture

Excel your commercial crop & livestock development through CRISPR precision genome editing

Comprehensively understand the regulatory guidelines for utilizing CRISPR gene editing as a novel breeding technique

Introducing the Inaugural CRISPR AgBio Precision Genome Editing CongressCommercialize the Next Generation of Sustainable & Superior CRISPR Generated Agricultural Products

Following positive regulatory opinion and the exponential uptake, CRISPR precision genome editing is having a significant impact in transforming agricultural breeding practices.

The CRISPR AgBio Congress is the first and only meeting dedicated to helping agricultural biotech and agrochemical companies leverage the power of CRISPR/Cas9 advanced trait breeding technology, to overcome productivity challenges, increase yield and pioneer sustainable agriculture for plant breeding, crop protection and livestock.

The application of faster and more accurate breeding techniques to achieve the most desirable traits is no longer hypothetical with the application of CRISPR/Cas9 driving trait discovery and selection to enhance yield, crop quality and disease resistance. At CRISPR AgBio Congress discover the latest technology advancements to improve efficiency, specificity and delivery of the CRISPR complex.

Ensure the successful application of CRISPR in functional genomics, trait discovery and in the advancement of genetically optimized plants, crops and livestock.

CRISPR AgBio Congress will give you clarity on the regulatory landscape and guidelines surrounding CRISPR technology and will highlight case studies on existing products receiving initial regulatory approval.

With consumer perception of gene editing playing a critical role in the success of commercialized CRISPR edited produce, this congress will address the steps you need to take to educate the wider public on the application of gene editing technology in agriculture.

Commercialize and take the next generation of sustainable and superior agricultural products to market faster and help meet the world’s growing food demands. Join the CRISPR AgBio Congress to harness precise genome editing and rapidly deliver accurate, controllable and planned changes to the genome you desire.

Dedicated Speaker FacultyGreg GocalChief Science Officer Cibus

Neil HoffmanChief Science Advisor, Animal and Plant Health Inspection Service & Biotechnology Regulatory ServicesUnited States Department of Agriculture (USDA)

Mike MendelsohnActing Chief, Microbial Pesticides Branch, Biopesticides and Pollution Prevention DivisionU.S. Environmental Protection Agency (EPA)

Federico Tripodi CEO Calyxt

Jin-Soo KimDirector & Professor, Center for Genome Engineering, Institute for Basic Science & Department of ChemistrySeoul National University

Mark CiganTrait Development DirectorGenus

Tom GreeneSenior Director, Research, Trait Discovery & OptimizationDuPont Pioneer

Joyce Van EckAssistant Professor, Director, Center for Plant Biotechnology ResearchBoyce Thompson Institute

Vonnie EstesGroup Leader, Agricultural and Industrial BiotechnologyCaribou Biosciences

Yiping QiProfessor, Department of Plant Science and Landscape ArchitectureUniversity of Maryland

Allan WenckHead of Trait Validation USBayer CropScience

Lanqin XiaPrincipal Investigator, Institute of Crop SciencesChinese Academy of Agricultural Sciences

Sergei SvitashevResearch ScientistDuPont Pioneer

Charlie ArnotCEOThe Center for Food Integrity

Cassie EdgarChief IP & Regulatory OfficerGenus

Yunde ZhaoProfessor, Cell and Developmental BiologyUniversity of California San Diego

Kan WangProfessor & Director, Center for Plant Transformation, Plant Sciences Institute, Department of AgronomyIowa State University

Todd BarkerSenior PartnerMeridian Institute







Conference Day One | Tuesday April 18, 2017

8.00 Registration & Coffee

9.00 Chair’s Opening Remarks

Excelling CRISPR Precision Genome Editing for Commercial Crop & Plant Development

Panelists:

Tom Greene, Senior Director, Research, Trait Discovery & Optimization, DuPont Pioneer

Vonnie Estes, Group Leader, Agricultural and Industrial Biotechnology, Caribou Biosciences

Mark Cigan, Trait Development Director, Genus

9.05 Panel Discussion: Extracting Maximum Value from CRISPR Genome Editing Technology to Advance Future Agriculture Get an exclusive insight into the latest developments within the CRISPR industry from one to one interviews with a panel of key opinion leaders, who are paving the way in taking CRISPR technology into the market for commercial product development within agriculture

This panel will discuss and answer the following questions:• What are the main areas within agriculture is CRISPR technology having the biggest impact?• What crops and plants are showing the most promise in terms of CRISPR complex delivery?• How does the CRISPR regulatory landscape in agriculture compare to other gene editing

tools and other fields?• What is the timeline for CRISPR technology entering the commercial market?• What is on the horizon for future CRISPR applications within agriculture?

Greg Gocal, Chief Science Officer, Cibus

9.50 Innovative Trait Development Tools in Plant Breeding will be Crucial for Doubling Global Agricultural Productivity by 2050• Rapid Trait Development System (RTDS™) employs Gene Repair OligoNucleobases

(GRONs) to make defined spelling changes in genomic DNA• We report that RTDS can significantly improve the outcome of double strand break activity

by reliably inducing precise and targeted nucleotide spelling changes closely aligned to the cut site

• Our work demonstrates the significance of gene editing to rapidly, precisely and reliably improve crop performance to develop any trait in commercially relevant crop varieties

10.20 Morning Refreshments & NetworkingJin-Soo Kim, Director & Professor, Center for Genome Engineering, Institute for Basic Science & Department of Chemistry, Seoul National University

11.20 Keynote Session: CRISPR Genome Editing in Plants & Animals• Digenome-seq enables assessment of genome-wide off-target effects of CRISPR systems

• Delivery of preassembled Cas9/Cpf1 RNPs reduces CRISPR off-target effects

• Cas9-coupled cytidine deaminase induces single-nucleotide substitutions in animals and plants

Advancing the Application of CRISPR/Cas9 in Functional Genomics for Trait Discovery in Plants & Crops

Yunde Zhao, Professor, Cell and Developmental Biology, University of California San Diego

12.05 Improvements of CRISPR/Cas9 & Their Applications in Auxin Biology• There are several challenges in using CRISPR for gene editing in plants: 1) efficient

production of gRNAs, 2) analysis of the mutations generated, 3) target sequence requirements, 4) simultaneously editing multiple target. We have developed strategies to overcome the aforementioned challenges

• Harnessing the nuclease activity of ribozymes to design an artificial gene RGR (Ribozyme-gRNA-Ribozyme) that produces an RNA molecule with ribozymes flanked at both ends of the designed gRNA. The produced gRNA efficiently guided Cas9-mediated cleavage of target DNA in vitro, in yeast, Arabidopsis, rice, and many other organisms

• With the RGR design, the target sequences are no longer limited to G(N)20GG or A(N)20GG because the method does not require the specific base for transcription initiation for gRNA production as was the case for U6 and U3 promoters

• Tandem RGR designs allow a single promoter to generate multiple gRNAs, thus allowing us to edit several genes in plants simultaneously and successfully use CRISPR technology to generate mutations in auxin genes

12.35 Networking Lunch




Yiping Qi, Professor, Department of Plant Science and Landscape Architecture, University of Maryland

13.35 Improving Multiplex CRISPR Systems for Plant Epigenome Editing• A multiplex TAL effector transcriptional activation system as a reference for improving CRISPR

activation systems• Improving multiplex CRISPR-Cas9 transcriptional activation by recruiting activators with RNA

binding proteins • Improving multiplex CRISPR-Cas9 transcriptional repression using tandemly fused repressors• An effective CRISPR-Cpf1 transcriptional repression system

Developing Delivery Methodologies to Enhance CRISPR/Cas9 Mediated Breeding

Sergei Svitashev, Research Scientist, DuPont Pioneer

14.05 Putting CRISPR-Cas to Work for Precision Breeding in Plants• Examples of genome editing in maize• Advantages of CRISPR-Cas9 delivery in the form of ribonucleoprotein (RNP) complexes• DNA-free gene editing in plants

Lanqin Xia, Principal Investigator, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences

14.35 Developing Delivery Methodologies to Enhance Genome Precise Modification for Crop Improvement Via CRISPR/Cas9 System• Development of a technology that facilitates gene replacement or targeted point

mutations will offer a great promise for harnessing plant genes for crop improvement• CRISPR/Cas9-mediated in planta gene editing to achieve homology-directed DNA repair

(HDR) in rice either through Agrobacterium or biolistic method, respectively• Targeted point mutations generated by a modified CRISPR/Cas9 system• Precise substitution amino acid residues in plant proteins through CRISPR/Cas9-

mediated HDR and base editing by a modified CRISPR/Cas9 system will expand our ability to modify genes that confer agriculturally important traits in crops

Kan Wang, Professor & Director, Center for Plant Transformation, Plant Sciences Institute, Department of Agronomy, Iowa State University

15.05 An Agrobacterium-Mediated Maize CRISPR Platform & a Proteolistics Delivery Method• To describe a public sector platform of Agrobacterium-delivered CRISPR/Cas9 for targeted

mutagenesis in maize is de and demonstrating the characterization of frequency and inheritability of mutations using four genes

• Harnessing the delivery method to introduce transgenic events in T0 generation for members of Argonaute 18 (ZmAGO18a and ZmAGO18b) and BEN1 (ZmBEN1a1 and ZmBEN1a4) with mono- and di-allelic mutations at the frequency ranging from 58% to 74% in the maize Hi II and B104 genotypes

• Direct delivery of proteins into plant tissues has advantage in genome editing, as this method will bypass DNA (transgene) integration into the plant genome, while generating precisely modified but truly “non-transgenic” plants

• An intracellular protein delivery methodology termed “Proteolistics” that utilizes a biolistic gun apparatus and involves a simple protein/projectile preparation step will be described

15.35 Afternoon Refreshments & Poster Session

Developing plant pharmaceuticals through CRISPR

gene editing

Harnessing CRISPR gene

editing to improve nutritional value

1 Communicating the benefits of

CRISPR technology to consumers

2 Advancing the commercialization

of CRISPR in agriculture

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16.20 Interactive CRISPR AgBio Roundtable Session In breakout roundtables, you will have the opportunity to catch up on the latest advancements within the field and learn from your fellow colleagues in this interactive format to optimize the use of CRISPR gene editing technology in agriculture. The topics that will be discussed are the following:

With an agenda packed with case studies and data-driven presentations, the CRISPR AgBio poster session will give you deeper access into the most innovative research. Meet leading CRISPR/Cas9 scientists and pick their brains to gain insights into the emerging gene editing applications within agriculture. Apply their learnings straight into your own work, cultivate research collaborations and leave inspired by new ideas.

17.20 Chair’s Closing Remarks

17.25 Close of Day One




Conference Day Two | Wednesday April 19, 2017

8.00 Registration & Coffee

9.00 Chair’s Opening Remarks

Addressing the Regulatory Pathway & Consumer Perception for CRISPR Gene Editing

Panelists:

Neil Hoffman, Chief Science Advisor, Animal and Plant Health Inspection Service/Biotechnology Regulatory Services, USDA

Mike Mendelsohn, Acting Chief, Microbial Pesticides Branch, Biopesticides and Pollution Prevention Division, U.S. Environmental Protection Agency

Cassie Edgar, Chief IP & Regulatory Officer, Genus

Todd Barker, Senior Partner, Meridian Institute

9.05 Panel Session Discussion: Navigating the Regulatory Environment for CRISPR as Novel Breeding Technique To provide more clarity on the current regulatory guidelines for utilizing CRISPR gene editing technology in the development of commercial products.

The panel will discuss and answer the following questions:

• What can be learned from the long history of safe use of random mutagenesis in the development of useful new crop characteristics?

• How might the outcomes achieved by new technologies like CRISPRs affect the regulatory profile in plants and livestock?

• Knock-outs vs. insertions - Addressing regulatory guidelines

• What are the current regulatory guidelines for utilizing CRISPR gene editing for livestock?

• What are the industry responsibilities in regards to educating the consumers and wider public on CRISPR edited commercial products?

• How to commercialize CRISPR/Cas9 agriculture products including crops and livestock

• Intellectual Property: How are people going to manage to operate and access technology?

Neil Hoffman, Chief Science Advisor, Animal and Plant Health Inspection Service/Biotechnology Regulatory Services, USDA

10.05 USDA Regulation of CRISPR-Modified Organisms • Regulatory guidelines facing non-transgenic genetically edited crops and plants• Case studies of products that have gone through the regulatory process such as waxy

corn and mushrooms• Regulatory status for non-GMO white bud, mushrooms and white seed corns

10.35 Morning Refreshments

Charlie Arnot, CEO, The Center for Food Integrity

11.05 Building Consumer Trust in Gene Editing• CFI’s peer reviewed trust model provides a template for building trust in gene editing • Consumer research on beliefs and attitudes toward technology provide insight into

consumer concerns and objections • The presentation will provide analysis and specific recommendations on how to build

consumer trust in gene editing

Taking CRISPR to the Market: Preparing for Translational Biotechnology

Vonnie Estes, Group Leader, Agricultural and Industrial Biotechnology, Caribou Biosciences

11.35 Exploring the Path of Commercialization in Agriculture• Supporting and developing appropriate regulatory frameworks • Building public acceptance and trust• Working in a global context• Who will profit and how will value be shared?

Tom Greene, Senior Director , Research, Trait Discovery & Optimization, DuPont Pioneer

12.05 Waxy Corn Case Study: Highlighting CRISPR/Cas9 Edited Plant & Crops Currently Under Development for Commercialization • How is CRISPR/Cas9 being utilized to modify plant and crop traits?• Outlining the regulatory process for utilizing CRISPR gene editing technology• How has CRISPR/Cas9 enhanced plant and crop breeding practices and outcomes?• Utilizing CRISPR gene editing to reduce costs and time to market




12.35 Networking lunch

Allan Wenck, Head of Trait Validation US, Bayer CropScience

14.00 Taking Gene Editing into the Agricultural Commercial Market• The ability to create precise mutations within plants has the potential to speed up

breeding and to bring traits to the market quicker than traditional mutagenesis approaches

• Precise targeting of the genome opens more flexible and efficient opportunities for stacking of traits

• Surgically precise modifications within multiple crop species is a challenge that requires multiple solutions to overcome

Joyce Van Eck, Assistant Professor, Director, Center for Plant Biotechnology Research, Boyce Thompson Institute

14.30 Application of CRISPR/Cas9 to Study the Role of Genes in Domestication & Improvement of Solanaceous Species• Efficacy of CRISPR/Cas9 in the Solanaceae• Traits related to domestication• Improvement of solanaceous species for specialty vegetable crops

Mark Cigan, Trait Development Director, Genus

15.00 Utilizing CRISPR Technology to Optimize Livestock Breeding Practices • How is CRISPR being adapted to enhance livestock breeding?• What are the producer profitability gains in using CRISPR to modify traits in livestock?

15.30 Chairman’s Closing Remarks

15.35 Close of CRISPR AgBio Congress

The conference is of great interest because it will cover the newest topics and the regulatory status related to CRISPR/Cas9

technology. I am especially excited for the opportunity to interact

with leaders in the field and the potential for collaborations

Boyce Thompson Institute, Speaker at

CRISPR AgBio 2017

The program highlights important and trending topics that are essential in mastering CRISPR technology for biotech applications.

The chance to meet and discuss with those who are at forefront of this

rapidly developing field makes attending this meeting even more exciting

University of Maryland, Speaker at

CRISPR AgBio 2017




CRISPR gene editing has transformed how academics and industry investigate molecular pathways through whole genome screening and functional genomics.

With the industry rapidly optimizing their CRISPR workflows for advanced trait discovery, join your peers at this dedicated interactive workshop to learn about the following:

• Optimizing CRISPR workflows for enhanced precision gene editing

• Highlighting examples of gene insertion, deletion, edit genes in plants and crops for functional genomics

• Enhancing CRISPR technology for whole genome screening and trait discovery

• Developing tissue culture methodologies and phenotyping in greenhouses or fields

Discover How to Harness CRISPR Gene Editing for Functional Genomics to Advance Trait DiscoveryDate: Thursday April 20, 2017 | Time: 9:00-12:00

Yunde Zhao received his Ph.D. in biochemistry from the University of Michigan and his postdoctoral training in plant genetics at the Salk Institute, where he was a Howard Hughes Medical Institute Fellow of the Life Sciences Research Foundation. Yunde’s group focuses on the plant hormone auxin, which regulates almost every aspect of plant growth and development. Yunde’s group is also interested in developing plant biotechnology. His group has made several keep discoveries and greatly contributed to our current understanding of auxin biology and genome editing. He identified the rate-limiting enzyme for auxin biosynthesis and played a leading role in establishing the first complete auxin biosynthetic pathway in plants. He showed that local auxin biosynthesis is important for plant development. The Ribozyme-based CRISPR gene editing technology developed by Yunde’s group allows cell and tissue specific gene editing. Yunde’s gene editing work invalidated the proposed functions of ABP1, a protein that has been studied extensively for decades.

Workshop leaderYunde Zhao, Professor, Cell and Developmental Biology, University of California

Post-Conference Workshop

Part of the CRISPR Event Series




Partners, Sponsors & Exhibitors

About Sponsorship

Cibus

Cibus is the leader in non-transgenic breeding & precision gene editing in agriculture. Our mission is to improve plants and other organisms with a new class of non-transgenic breeding technologies. This work is carried out without integrating foreign genetic material thus the resulting organisms are non-transgenic. Since our improvements are non-transgenic, they will be globally acceptable.

• Present and position your capabilities to key decision makers that are actively seeking new solutions to optimize lead candidates

• Differentiate yourself from your peers and demonstrate your specific expertise and technology

• Meet all the business contacts you need across 2 days – receive dedicated 1-2-1 networking exposure with your target audience

Find a solution to support your business strategy:There is no one size fits all approach to partnership at CRISPR AgBio 2017. Your business has specific goals this year. When you work with us we will build a partnership that helps you achieve those aims. Partnership options could include a presentation within the main program, hosting an evening networking reception, exhibiting, 1 hour breakfast briefing, workshop or hosting a lunch with a targeted group. Contact Diane McKenna to discuss your requirements and build a partnership to deliver the results you need.

*Anticipated Attendance

Program Partner www.cibus.com

You’ll Meet People From... Companies Who Are Attending

Why a partnership with CRISPR AgBio 2017 will benefit your business:

Contact

Diane McKenna Commercial Director


Become a Commercial Partner of CRISPR AgBio Congress

Directors

Lab Head & Group Leaders

Senior Scientists

Professors/Principal Investigators

Seed Companies

Universities & Research Institutes

Gene Editing Specialist Biotechs

Solution & Service Providers

Attendance By Sector

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20%

10%

Typical Attendee Seniority

25%

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20%30%

PricingRegisterwww.crispr-agbio.com/register Tel: +1 212 537 5898 Email: [email protected]: Hanson Wade 4th Floor, 52 Grosvenor Gardens, London, SW1W 0AU

• 10% discount – 3 delegates • 15% discount – 4 delegates • 20% discount – 5 or more

delegatesPlease note that discounts are only valid when three or more delegates from one company book and pay at the same time.

Team discounts are not applicable to academic pricing.

Team Discounts* Top 3 Benefits of Attending

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Discover key technology advancements to enhance your application of CRISPR editing

Learn how the industry are utilizing CRISPR as a tool to optimize trait discovery

Explore the commercial uses of CRISPR in plant, crop & animal breeding

Venue

Sheraton San Diego Hotel & Marina 1380 Harbor Island DriveSan Diego, California92101, USA

www.sheratonsandiegohotel.com

Full payment is due on registration. Cancellation and Substitution Policy: Cancellations must be received in writing. If the cancellation is received more than 14 days before the conference attendees will receive a full credit to a future conference. Cancellations received 14 days or less (including the four-teenth day) prior to the conference will be liable for the full fee. A substitution from the same organization can be made at any time.

Changes to Conference & Agenda: Hanson Wade reserves the right to postpone or cancel an event, to change the location or alter the advertised speakers. Hanson Wade is not responsible for any loss or damage or costs incurred as a result of substitution, alteration, postponement or cancellation of an event for any reason and including causes beyond its control including without limitation, acts of God, natural disasters, sabotage, accident, trade or industrial disputes, terrorism or hostilities.

Data Protection: The personal information shown and/or provided by you will be held in a database. It may be used to keep you up to date with developments in your industry. Sometimes your details may be obtained or made available to third parties for marketing purposes. If you do not wish your details to be used for this purpose, please write to: Database Manager, Hanson Wade, Suite A, 6 Honduras Street, London EC1Y 0TH

TERMS & CONDITIONS

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Industry Pricing

Register & Pay before Friday January 20, 2017 Standard Prices

Gold PackageConference + Workshop

$1998 (save $800) $2598 (save $200)

Silver PackageConference Only

$1599 (save $600) $2199

Workshop $599

Academic Pricing

Register & Pay before Friday January 20, 2017 Standard Prices

Gold PackageConference + Workshop

$1398 (save $650) $1848 (save $200)

Silver PackageConference Only

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Workshop $399




Excellent mix of industry, vendors, and academia, all with different perspectives

Past Attendee of CRISPR Congress Series, Sanofi-Genzyme