CTRF Leadership Meeting

February 4, 2002

Institutional Partners

Develop Infrastructure and Intellectual Property that

Enhances the Competitiveness of the Partners for Clinical and

Extramural Funds

Evaluate gene expression (and genetic changes) in human brain, ovarian, breast and hematopoetic cancers

Link gene expression (and genetic changes) to clinical findings and clinical laboratory findings (including histopathological diagnoses) in a common database

Evaluate linked data using bioinformatics

ChandhokeGrant

ChristensenFryxell

Jamison

Torr (Central Admin)GinderGarrettBuck

Guiseppe-ElieAbraham

Cooper

Year 1 Year 1 Year 1

$325,000 $582,000 $93,000

Total (3 yrs) Total (3 yrs) Total (3 yrs)

$975,000 $1,734,603 $290,397

CTRF CG Administrative Assistant (Jo Ann Breaux) will monitor VCU ledger 5 and cost share accounts.

Plan is to create regular feedback to project PIs in future.

Cost share expenditures not paid from cost share linked ledger 4 account must be documented using ‘In Kind/3rd Party Cost Share form’ obtained from Margie Booker’s office.

• Due 12/31/01• Submitted

12/28/01• Sections

– Purpose– Background– Research Plan

• Infrastructure Development– Progress Report– Specific Reportables

CTRF

BenignBenign (not growing)

vs.BenignBenign

(not growing)

vs. BenignBenign (growing)

vs. CancerCancer (growing)

Figure 5: Example GeneChip Result(DNA Chip)

Figure 6:Analysis: Total genes probed: ~12,500Expressed: ~4,500Not expressed: ~8,000

Figure 7: An example of a spotted DNA microarray with two-color detection of hybridization of probe (surface) with target (sample). The two sectors of 16 x 20 elements is shown following hybridization with two differentially labeled cDNA preparations, Cy3 (pseudocolored green) and Cy5 (pseudocolored red). The overlaying of the green and red images produces the image shown. The hue of each spot, ranging from green to red, indicates the relative expression level for the gene specific for each spot. (Image courtesy Packard Biochip Technologies).

UI-R-E1-fd-b-03-0-UI3.34 fold increaseMyogenic regulatoryfactor (MyoD) gene

UI-R-A1-ee-d-06-0-UI2.8 fold increaseSpongiotrophoblastspecific protein (SSP)

Figure 9: Over two fold increase in gene expression

Figure 8: H4IIE cells exposed to 10g/ml JP8 Jet fuel over 2 hours.

Establish Focus Groups to address each of the basic tasks.

Each Focus Group to consist of professionals associated with the CTRF Project with special expertise or knowledge in one or more areas required to address basic tasks.

Structure work within Focus Groups to deal with interrelatedness of basic tasks.

Tissue Bank Clinical and Pathology Laboratory

Data Database Design Data Analysis Quality Assurance in Microarray

Analysis (Chip Fabrication - proposed)

Focus Group Leaders

GMU

G e rald in e G ran t ( G M U )S u ha il N as im ( V C U )B a rr ie C o o k ( I n o va)

Tissue Bank

L yn ne P en b e rth y (V C U )S u ha il N as im ( V C U )

J a m e s C o op e r ( I n o va)J a m e s B u rge s s ( I no va)

B a rr ie C o o k ( I n o va)

C linP ath

C u rtis J am is on ( G M U )L yn ne P en b e rth y (V C U )

G re g M ille r (V C U )M ike S he ride n ( I no va)

D B D esign

V ika s C h an d h oke ( G M U )G re g B u ck ( V C U )

D ataA nalysis

A la n C h r is ten s en ( G M U )A n d re a F e rre ira - G o n z ale z (V C U )

Q A / LQ C

VCU I nova

• Tissue Acquisition System (TAS) Protocol Completed

• Submitted to MCC Protocol Review & Monitoring Comm

• Contents

– Tissue Handling

• residual sample

• obtained after pathology review

– IC - Obtain prior to & following surgery

– Primary Data Collection

• First Tissue Bank Staff member hired.

Establish standing weekly

or biweekly meeting dates

and times

CG-TISBK: Tissue Bank CG-CLNDT: Clinical and Pathology Data CG-DBDSN: Database Design CG-ANLDT: Analyze Data (Data Analysis) CG-QAQC: QAQC CG-LDRPI: Focus Group Leaders and PIs CG-MEMBS: All Members

Address messages to: listname@VENUS.VCU.EDU

Unsubscribe to the listserv by submitting a message with the words SIGNOFF listname to: LISTSERV@VENUS.VCU.EDU

Subscribe to the listserv by asking the PI with whom you work to submit your name and E-mail address to the Program Director (C.Garrett)

USE the listserv(s) to inform members of your activity or to seek advice from the members.

Intellectual property reporting - licenses, patents, etc

Publications

New applications identified - CTRF Administrative office

will search for new funding opportunities (SPIN)

will collect CVs, other support, facilities, interest documents

goal - 4 - 8 million in d.c. from CTRF CG Project

Any other discoveries

Federal money leveraged

Private research money leveraged

Advancement of technology and economic development in VA

Old Business

New Business

MondayMarch 4, 20029:30 am