damjana drobne university of ljubljana, biotechnical faculty department of biology , slovenia
DESCRIPTION
CONFERENCE ON NANO-SAFETY 22.-24. April 2009, Ljubljana, Slovenia. Nanosized ZnO and nano TiO 2 : bioavailability and bioaccumulation. Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA E-mail: Damjana.Drobne@ bf. uni-lj.si. - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/1.jpg)
Damjana Drobne University of Ljubljana, Biotechnical FacultyDepartment of Biology, SLOVENIA
E-mail: [email protected]
Nanosized ZnO and nano TiO2:
bioavailability and bioaccumulation
CONFERENCE ON NANO-SAFETY22.-24. April 2009, Ljubljana, Slovenia
![Page 2: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/2.jpg)
Research group for nanobiology and nanotoxicology
Dept. Biology, Biotechnical faculty, Uni Ljubljana
Damjana Drobne; Vladka Lešer; Janez Valant; Živa Tkalec Pipan;Marjetka Kralj Kunčič; Sara Novak, student; Tea Romih; student ______________________________________________
National Institute of chemstryAnita Jemec ______________________________________________
Faculty for computer and information science, Uni LjubljanaJernej Zupanc
![Page 3: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/3.jpg)
J1-9475 (C) Elaboration and evealution of a single species toxicity test for nanoparticles with a terrestrial isopod evaluation and Izdelava in vrednotenje testa strupenosti (basic research project), Slovenian Research Agency 2006-2009
![Page 4: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/4.jpg)
Aim of work
…. to study interactions between nanoparticles and biological systes
![Page 5: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/5.jpg)
A terrestrial isopod, a model invertebrate organism
In vivo and in vitro biological system
Lipid vesicles
![Page 6: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/6.jpg)
... studding toxicity of metal pollutants and pesticides present in the environment.
Our past experiences
![Page 7: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/7.jpg)
Present challanges
Interactions between nanoparticles and biological systems.
![Page 8: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/8.jpg)
.. .biological potential of nanosized metal oxides
+
Photo: M. Bele, Institute of chemistry, Ljubljana
![Page 9: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/9.jpg)
Chemicals affect target molecules or interfere with different intracellular compartments. The effect is propagated upwards to higher levels of biological complexity (cells, tissue, organs, organism) and it is manifested as toxic response.
![Page 10: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/10.jpg)
What about nanoparticles?
![Page 11: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/11.jpg)
1) One hypothesis is that nanoparticles interact firstwith cell membrane!
A consequesnce is….
![Page 12: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/12.jpg)
… cells are affected, but nanoparticles were not detected inside the cells (nanoTiO2).
Photo: J. Valant
![Page 13: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/13.jpg)
2) The other opened question is: are nanoparticle bioavailable; do they bioaccumulate?
By L.Ziccardi, M. McArdle, Y. Lowney, J. Tsuji
?
![Page 14: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/14.jpg)
Do ZnO nanoparticles accumulate?
![Page 15: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/15.jpg)
Our work
- model organism, terrestrial isopod P. scaber
- food exposure to nanoparticles for 4 weeks
- comparison among bioaccumulation of - nano ZnO, - macropowder ZnO
- ZnCl2
![Page 16: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/16.jpg)
Background
• terrestrial isopods accumulate high amounts of meals (Zn, Pb, Cu, Fe etc). in their tissue
• a lot of knowledge already exist on the accumulation pattern and accumulation strategy of metals if offered as soluble salts
-
![Page 17: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/17.jpg)
metal analyses in whole organism by AAS
+Four weeks feeding on Nanoparticel, macropowsder or ZnCl dosed food
concentrations of Zn in the body
Experimental set up
![Page 18: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/18.jpg)
Background knowledge: FIB/SEM,
![Page 19: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/19.jpg)
Photo: F.Tatti, FEI
![Page 20: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/20.jpg)
Scanning electron micrograph of macropowder of ZnO.
Scanning electron micrograph of nanosized ZnO
![Page 21: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/21.jpg)
Results
• no diffrences in amount of accumulated Zn among groups
![Page 22: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/22.jpg)
control Zn2000n zn2000M znCl2000 Zn5000n zn5000M ZnCl50000
200
400
600
800
1000
1200
$
&
& glede Zn 2000 M $ glede ZnCl 2000
*
*
# glede Zn 2000 n * glede na kontrolo
Exposure concentration (g/g food)
Am
ou
nt o
f Z
n (g
/g d
ry w
eig
ht)
TOTAL BODY
*#
By Ž. Pipan Tkavec, D. Drobne, A.Jemec ,T.Romih, P.Zidar, M.Bele
2000 µg/g
5000 µg/g
![Page 23: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/23.jpg)
Discussion
• we are not able to answere the question about nanoaprticle bioavailability or bioaccumauation pottential
• the fact is that when nanosized or macrosized ZnO is ingetsed an equal prtion of Zn is accumulated as when ZnCl2 is inegested
![Page 24: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/24.jpg)
Present/Future direction of research
• investigation of accumulation of other nanparticles with lower dissolution rates; for example Cu and Ag nanoparticles
• Investigation of interaction between membranes and nanoparticles
![Page 25: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/25.jpg)
Photo by J.Zupanc
Computer aided analysis of lipid vesicles shape transformations after incubation with nanoparticles.
![Page 26: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/26.jpg)
Publications:
- DROBNE, Damjana, JEMEC, Anita, PIPAN, Živa. In vivo screening to determine hazards of nanoparticles: nanosized TiO2. Environ. pollut. ,2009, issue 4, vol. 157, str. 1157-1164
- JEMEC, Anita, DROBNE, Damjana, REMŠKAR, Maja, SEPČIĆ, Kristina, TIŠLER, Tatjana. Effects of ingested nano-sized titanium dioxide on terrestrial isopods Porcellio scaber. Environ. toxicol. chem., 2008, vol. 27, no. 9, str. 1904-1914,
- JEMEC, Anita, DROBNE, Damjana, TIŠLER, Tatjana, SEPČIĆ, Kristina. Biochemical biomarkers in environmental studies-lessons learnt from enzymes catalase, glutathione S-transferase and cholinesterase in two crustacean species. Environ. sci. pollut. res. int., 2009, 11 str.,
![Page 27: Damjana Drobne University of Ljubljana, Biotechnical Faculty Department of Biology , SLOVENIA](https://reader035.vdocument.in/reader035/viewer/2022062314/56814816550346895db54453/html5/thumbnails/27.jpg)
Thank you for your attention!