data extraction cdna arrays affy arrays. stanford microarray database

29
Data Extraction cDNA arrays Affy arrays

Post on 21-Dec-2015

222 views

Category:

Documents


1 download

TRANSCRIPT

Page 1: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Data Extraction

cDNA arrays

Affy arrays

Page 2: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Stanford microarray database

Page 3: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Go into public login select organism and the author

Page 4: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

If you chose to display data, you can access raw data

Page 5: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

If you chose to display data, you can access raw data

Page 6: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Display data for each slide and download

Page 7: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Or you can chose to data retrieval

Page 8: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Click on data analysis to select experiments to download

Page 9: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Click on data retrieval, use default selections, select gene name, accession etc.

Page 10: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Proceed to data filtering

Page 11: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Retrieve data

Page 12: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Download preclustering file: unnormlalized data

Page 13: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Save unnormalize data as text file

Page 14: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Open data file in excel

Page 15: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Proceed to gene filtering

Page 16: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Click on filter data and download median normalized data

Page 17: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

You may chose to download raw data for channel 1 and channel 2 to do other normalizations

Page 18: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Affymetrix Data: Go to GEO

Page 19: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Type in a keyword and select a study

Page 20: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Experimental Series Information

Page 21: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Pubmed

Page 22: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Individual array information

Page 23: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Individual array information

Page 24: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Some have .cel files available as suplementary materials

Page 25: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Other examples of sample files normalized differently: e.g., MAS5

Page 26: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Other examples of sample files normalized differently: e.g., dchip

Page 27: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Other examples of sample files normalized differently: e.g., rma

Page 28: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

You can download the normalized or raw (.cel file) data

Page 29: Data Extraction cDNA arrays Affy arrays. Stanford microarray database

Normalization Methods for raw data

Double channel cDNA (channel intensities)

Affymetrix oligo arrays (.cel files)