depliant 2011 - sept.11
DESCRIPTION
Depliant products Biosigma 2011TRANSCRIPT
Graphic Project : Biosigma S.r.l. 092011 - International Depliant - Ver. 5.5 - Ed. September 2011
4
BIOSIGMADisposable Labware for Life Science
5
We are here!
Since 1988 Biosigma S.r.l. produces and distributes disposable plastic items for laboratory of analysis and the study of biotechnology.In our over 12.000 sqm facilities, we dispose of latest injection machines and informatics tools that allow production of high standard quality articles sill being competitive on the worldwide market.In respect of environment, in 2010 it has been installed a photovoltaic system that covers 100% of required energy.Our efforts in research and development allow us to offer a growing and more advanced range of products.Every customer can count on a perfectly organized structure, ISO 13485 certified, able to adapt products and services to continuous demands of market. In 2006 it has been created a Clean Room to manufacture products free of: human DNA, DNasi, RNasi, Pyrogens and ATP. In 2008 Storage and Shipping system has been computerized. In 2009 it has been created a dedicated area for the automatic packaging of products.Our products are distributed in over 60 countries in the world, directly from our warehouse or from our national and international distributors.Our staff attends the major trade shows in the world, included Medica Exhibition in Düsseldorf (Germany), Arab Health in Dubai, Pittcon and AACC in U.S.A.Biosigma is also active with regard to the social as for example in sport. In fact it is committed to support the inclusion of youth in various sports associations, providing its names to promote their activities.We offer our customers experience and professional competence; customer satisfaction and quality of products are always our main targets.
August 2011 Dominique Dutscher SAS acquired the majority share capital of the Biosigma group (Biosigma SRL in Italy , Stirilab SRO in Slovakia and MUF-PRO SRO in Czech Republic).Dominique Dutscher SAS , a French group of companies , is a leading company in distribution of consumables and equipment for the life science and biotechnology market. As you can see on the Dutscher website ( www.dutscher.com) , the group offers over 30 000 items of about 140 very well known manufacturers to its customer base.Biosigma continues to operate under its name as a separate company carrying forward its business with the same philosophy as since ever, giving first emphasis to fulfill its customer’s needs.A large part of the Dutscher offering will also be available through the Biosigma channel.
Contacts:BIOSIGMA S.R.L. Via Valletta, 630010 Cantarana di Cona (VE) • ITALYphone 0039/0426 30 22 24fax 0039/0426 30 22 28skype biosigma_stefaniae-mail [email protected] www.biosigma.com
Follow us on :
Biosigma S.r.l.a Dominique Dutscher CompanyThe italian brand synonymous of quality and innovation in respect of environment.
Adherent to:
BIOSIGMADisposable Labware for Life Science
6
CryoGen® Tubes CLEARLine®Increase productivity with the CryoGen® vials, workstations and storage boxes.The Biosigma CryoGen® vial system is designed for cryogenic storage of biological specimens at temperatures as low as -196°C. This innovative high-purity line features outstanding leak resistance with a rubber o-ring molded into the screw-cap. Vials are manufactured in accoradance with the quality guidelines ISO 13485:2004 and ISO 14644 and 14698 (Federal Standard 209) in a Class 7 cleanroom (Class 10,000). These vials are certi-fied to be DNA, DNase, RNase, pyrogen and ATP free by an indipendent external american laboratory*. Each lot is sterilized by beta radiation**. Small-lot packaging ensure sterility and usage control.On each tube is printed a unique bar-code, with replaces the old labels for storage. Further quality checks are performed by our laboratory. Tubes made in medical polyethylene, stoppers in medical polypropylene; these raw materials are tested in according to “the United States Pharmacopoeia” (USP) and with a “Drug Master File” (DMF) at the FDA.*Certificate of Analysis and Certificate of Quality availabe upon request.**Beta radiation sterilization performed in accordance with EN ISO 11137.
External thread screw cap
Printed graduation
Interlocks to workstation
Internal thread screw cap
White, writing sur-face for specimen identification.
For improved handling and safety of biotechnology materials
• Vial made from medical polypropylene, cap made from medical polyethylene and rubber, both are autoclavable
• Single-body, single-turn screw cap designed with internal or ex-ternal threads
• Select from self-standing and round bottom vials • Printed graduations for accurate measurements • Large white area for writing specimen identification• Sterile• Availability of certificates of analysis for lot• Bar code printed directly on tubes
CLEARLine®Biosigma S.r.l. CERTIFY That ClearLine® products are completely free of :• human DNA • DNase • RNase • Pyrogens • ATP• The control and the certification is done by an external laboratory.• The absence of human DNA is verify with a PCR assay which is able to find the presence of human DNA with a
sensibility of 1 pg. • The absence of DNase and RNase enzymes is verified incubating on the products, reaction swabs containing DNA
and/or RNA at a temperature of 37°C. The absence of degradation is after verified by electrophoresis on gel.• To certify the absence of Pyrogens, the LAL Test is used. • The presence of ATP is verified with a bioluminescence method exploiting the reaction between the luciferase
enzyme and its luciferin substrate and and the adenosin-tri-phosphate molecule (ATP).Biosigma S.r.l. GUARANTEES The leak-proof of the product at a pressure of 95 kPa ( 0,95 bar, 14psi ), by applying a closure force of cap of not less than 8 cNm.
NEW MARIO and MARIA CAPS
Our revolutionary and innovative caps are manufactured with bi-injection system to avoid any contamination and liquid leak since the rubber o-ring is injected directly into the cap during the moulding.
Ø 12,20 mm Ø 14,20 mmMARIO Internal thread MARIA External thread
Barcode
Barcode
Barcode printed directly on the tube. Ideal for automated storage samples.It can be read with the most common ba code readers.
CryoGen® TubesFeatures:
BIOSIGMADisposable Labware for Life Science
7
Ref. CL1ARBIPS CL2ARIPS CL2ARBIPS CL4ARIPS CL4ARBIPS CL5ARIPS CL5ARBIPSVolume 1,0 ml 2,0 ml 2,0 ml 4,0 ml 4,0 ml 5,0 ml 5,0 ml
Height cm 4,27 4,70 4,82 7,49 7,62 8,92 9,03
Style self standing round botton self standing round botton self standing round botton self standing
Packaging 1000 units, 100/bag 10 bag/case
1000 units, 100/bag 10 bag/case
1000 units, 100/bag 10 bag/case
1000 units, 100/bag 10 bag/case
1000 units, 100/bag 10 bag/case
1000 units, 100/bag 10 bag/case
1000 units, 100/bag 10 bag/case
Capdisk• Easily snap into screw-top caps• Ideal for color coding samples
Ref. Color
CRY910/AZ Light blue
CRY910/BI White
CRY910/G Yellow
CRY910/RA Pink
CRY910/RO Red
CRY910/V Green
Ref. CL1ARBEPS CL2ARBEPS CL2AREPS CL3ARBEPS CL4ARBEPS CL5ARBEPSVolume 1,0 ml 2,0 ml 2,0 ml 3,0 ml 4,0 ml 5,0 ml
Height cm 4,10 4,45 4,59 6,92 7,36 8,76
Style self standing self standing round botton self standing self standing self standing
Packaging 1000 units, 100/bag 10 bag/case
1000 units, 100/bag 10 bag/case
1000 units, 100/bag 10 bag/case
1000 units, 100/bag 10 bag/case
1000 units, 100/bag 10 bag/case
1000 units, 100/bag 10 bag/case
CryoGen® Tubes with New MARIA external thread capNew with Barcode
CryoGen® Tubes with New MARIO internal thread capNew with Barcode
• 40 holes.• In Polycarbonate.• Availabe in 3 colors.• Autoclavable.
Ref. Color Dimension cm
BSM5810/AZ Blue L 21,5 P 10,0 H 2,50
BSM5810/RO Red L 21,5 P 10,0 H 2,50
BSM5810/V Green L 21,5 P 10,0 H 2,50
Workstation for CryoGen® Tubesfor 1 - 2 - 3 - 4 - 5 ml tubes
BIOSIGMADisposable Labware for Life Science
8
Ref. Grid Color Dimension. mm
BSM58061/AZ light blue 132x132x52
BSM58061/RO red 132x132x52
BSM58061/V green 132x132x52
Ref. Grid Color Dimension. mm
BSM58071/AZ light blue 132x132x94
BSM58071/RO red 132x132x94
BSM58071/V green 132x132x94
for 1 - 2 ml tubes, external and internal thread
for 3 - 4 - 5 ml tubes, external thread and 5 ml internal thread
CryoGen® Box 25
Ref. Grid Color Dimension. mm
BSM58025/AZ light blue 76x76x52
BSM58025/RO red 76x76x52
BSM58025/V green 76x76x52
for 1 - 2 ml tubes, external and internal thread
CryoGen® Box
Ref. Grid Color Dimension. mm
BSM58100/AZ light blue 132x132x52
BSM58100/RO red 132x132x52
BSM58100/V green 132x132x52
for 1 - 2 ml tubes, internal thread
Ref. Grid Color Dimension. mm
BSM58081/AZ light blue 132x132x52
BSM58081/RO red 132x132x52
BSM58081/V green 132x132x52
Ref. Grid Color Dimension. mm
BSM58091/AZ light blue 132x132x94
BSM58091/RO red 132x132x94
BSM58091/V green 132x132x94
CryoGen® Box 81 LOW
CryoGen® Box 81 HIGH
CryoGen® Box 100
for 1 - 2 ml tubes, external and internal thread
for 3 - 4 - 5 ml tubes external thread and 5 ml internal thread
CryoGen® Box 81 LOW Engraved
CryoGen® Box 81 HIGH Engraved
Features:Boxes in polycarbonate for CryoGen® Tubes storage. Different models are available to accommodate either 25, 81 or 100 Cryovial® tubes. These durable cryogenic storage boxes are designed to be used at temperatures between -196°C and +121°C and are autoclavable at 120°C, for 20 min-utes. A transparent cover with a pad printed or engraved numbering allows the user to see the contents of the box, and is keyed to the base to prevent misalignment. 25 and 81 places have a colored grid (Picture 1) that can be jointed with the bottom (Picture 2). Grids and lids have a double 45° edge (Picture 3) to make closure easier. 100- place boxes have special divider (Picture 4) that allow to maximize the pace. 3 colours available: Light blue, Red and Green.
Picture 1 Picture 2 Picture 3 Picture 4
Serigraphed
Serigraphed
Serigraphed
Serigraphed
Engraved
Engraved
BIOSIGMADisposable Labware for Life Science
9
Microtubes with screw cap• Biosigma’s microcentrifuge tubes are manufactured from medical-grade polypropylene to exceed the exacting standards demanded by
researchers. • Six styles of caps to choose from and three sizes of conical bottom or self-standing tubes (0.5, 1.5 and 2ml).• Biosigma’s microcentrifuges withstand high speed centrifugation up to 25.000g and temperature from -80°C to +121°C.• Autoclavable to 121°C. • Tubes made in medical polyethylene, stoppers in medical polypropylene; these raw materials are tested in according to “the United States
Pharmacopoeia” (USP) and with a “Drug Master File” (DMF) at the FDA.
Ref. Volume(ml)
Height (cm)
Bottom O-Ring Graduation / Writing area
Sterile Packaging
BSM724/S 1,5 4,67 conical x x x 1000 pcs, 5 bags x 200 pcs
BSM734/S 1,5 4,73 self standing x x x 1000 pcs, 5 bags x 200 pcs
Ref. Volume(ml)
Height (cm)
Bottom Graduation / Writing area
Packaging
BSM600 0,5 4,69 conical 3000 pcs, 3 bags x 1000 pcs
BSM610 0,5 4,69 self standing 3000 pcs, 3 bags x 1000 pcs
BSM720 1,5 4,77 conical x 3000 pcs, 3 bags x 1000 pcs
BSM730 1,5 4,77 self standing x 3000 pcs, 3 bags x 1000 pcs
BSM740 2,0 4,77 conical x 3000 pcs, 3 bags x 1000 pcs
BSM750 2,0 4,77 self standing x 3000 pcs, 3 bags x 1000 pcs
Microtube 0,5 ml
Microtube 1,5 ml
Microtube 2 ml
Tubes
Ref. Volume(ml)
Height (cm)
Bottom O-Ring Graduation /Writing area
Sterile Packaging
BSM604/S 0,5 4,67 conical x x 1000 pcs, 5 bags x 200 pcs
BSM614/S 0,5 4,73 self standing x x 1000 pcs, 5 bags x 200 pcs
Ref. Volume(ml)
Height (cm)
Bottom O-Ring Graduation / Writing area
Sterile Packaging
BSM744/S 2,0 4,69 conical x x x 1000 pcs, 5 bags x 200 pcs
BSM754/S 2,0 4,73 self standing x x x 1000 pcs, 5 bags x 200 pcs
Ref. Color
BSM910/AZ Light blue
BSM910/BI White
BSM910/G Yellow
BSM910/RA Pink
BSM910/RO Red
BSM910/V Green
All microtubes can be diversified by using color code inserts, as follows:
Dischetti colorati / Cap-Disks
Color Standard flat screw cap
Screw cap suitable for cap disk
Screw cap suitable for cap disk with collar
Standard flat screw cap + O-Ring
Screw cap suitable for cap disk + O-Ring
Screw cap suitable for cap disk with collar + O-Ring
cm High 6,90 8,30 8,30 6,90 8,30 8,30
cm Diameter 1,34 1,34 1,34 1,34 1,34 1,34
Neutral BSM800 BSM801 BSM802 BSM810 BSM811 BSM812
Caps for microtubes
BIOSIGMADisposable Labware for Life Science
10
Microtubes
BSA021Microtube without cap .H 3,90cm Ø 1,80cm
BSA022Microtube with Flat Cap Utilizable on EPPENDORF ® Vol 1,5 ml graduated in polypropylene, non sterile. Rotation speed centrifuge 16,400 rpm - 25,000 x g. Autoclavable for 20 min at 121 ° C.H 4,19cm Ø 1,80mm
Ref. Color
BSA022 neutral
BSA022/BLU blue
BSA022/G yellow
BSA022/RA pink
BSA022/V green
BSA0269Microtube in polypropylene Vol. 2ml With flat cap, non sterile.H 4,19cm Ø 1,80cm
• Flat cap adapted for easy writing
• Excellent transparency
• Evident graduation
• Great seal in the closing phase
DD016131PCR microtube 0,2ml with domed cap in PP, natural. Dnase and Rnase free not sterile.H 2,30cm Ø 0,64cm
BSA0251PCR microtube 0,2ml with flat cap in PP, natural. Dnase and Rnase free, not SterileH 2,17cm Ø 0,64cm
DD391233Microtube Vol. 0,5 ml, with flat cap in polypropylene.H 3,16cm Ø 0,77cm.
BSA0261PCR microtube 0.5ml with flat cap in PP, natural. Dnase and Rnase free, not sterile.H 3,16cm Ø 0,77cm.
Ref. Color Dim. cm
BSM5827/AZ Blue L 18,5 P 12,0 H 5,50
BSM5827/RO Red L 18,5 P 12,0 H 5,50
BSM5827/V Green L 18,5 P 12,0 H 5,50
4-Ways Rack
• Top level : Last line of holes (top level) is also a workstation for microtubes so they can be opened and closed just using one hand. Thanks to the locking system the tubes securely locks and not turn.
• Third level: Rack for 1,5 ml and 2 ml microtubes snap cap and screw cap microtubes.• Second level: Rack for 0,5 ml microtubes snap cap• Lower level: Rack for 0,2 ml microtubes snap cap
Rack for microtubes snap and screw cap
BIOSIGMADisposable Labware for Life Science
11
E.s.r. SystemBSV012BIO-VES, E.S.R. glass pipettes, graduated, with piston
BSV011E.S.R. RACK 10 Places in ABS
BST128E.S.R. TUBE with 0,25 ml NACitrate, for 1 ml of blood, Ø 12 x 56 mm, pink stop-per, labelled, with no pierceable stopper, in polypropylene.
BSV0125E.S.R. TUBE with 25 ml of Na Citrate, Ø 12 x 66 mm, cylindrical, pierceable stopper, labelled, in polypropylene.
BSV022
BSV023
BSV022Rack 10 Places in PS for 12 mm diam test tubes
BSV023Rack 10 Places in PS for 13 mm diam test tubes
Accessories for inclusion
Biopsy
Ref. Color
BSI103 white
BSI1032 yellow
BSI1034 pink
BSI1036 green
BSI1037 orange
BSI1038 light blue
Hystology
Ref. Color
BSI102 white
BSI1022 yellow
BSI1024 pink
BSI1026 green
BSI1027 orange
BSI1028 light blue
BSI105 Histological Sponge.H 3,00 L 2,50
Histology cassettesCassettes in Pom detachable cover 40x7,0x28 mm with rettangular holes 5,0x1,0 mm.
Biopsy cassettesCassettes in Pom detachable cover 40x7,0x28 mm with square holes 1,0x1,0 mm.
BIOSIGMADisposable Labware for Life Science
12
Microtips, Pasteur Pipettes
Ref. BSV136 BSV134 BSV140 BSV150 BSV143 BSV180Description 3 ml
Graduated1 ml
GraduatedFor
Fast Read kit For Card Test For M.T. kit Pasteur Pipette 10 ml
Pack Size 6 dispenser x 500 pcs
6 dispenser x 500 pcs
6 bag x 1000 pcs
10 bag x 1000 pcs
6 bag x500 pcs
6 bag x 250 pcs
Lenght mm 155 145 125 70 148 160
Graduation 0,5 ml 0,25 ml None None None None
Bulb draw 3,5 ml 3,5 ml 1 ml 1 ml 1 ml 10 ml
P.S. Volumes are only indicative. These devices have no primary-function of analytic measuring.
Non sterile
SterileBSV13511ml Pasteur Pipette sterile, individual peel pack
BSV1371 3ml Pasteur Pipette sterile, individual peel pack
BSV1351ml Pasteur Pipette sterile, sterile peel pack of 5 pcs.
BSV1373ml Pasteur Pipette sterile, sterile peel pack of 5 pcs.
BSD335 Plastic housing cassettes for 3.9 mm strips free writing
BSD330 Plastic housing cassettes for 3.9 mm strips with HCG writing
BSD310 Plastic housing cassettes for 5 mm strips free writing
BSD300 Plastic housing cassettes for 5 mm strips with HCG writing
BSV150 Pipette pasteur for card test-kit 40 µl
Card-Test Accessories
BIOSIGMADisposable Labware for Life Science
13
BSA81Cover tray reagents utilizable on Cobas Mira® Small- 4 ml.H 39,5 Ø 16,42 mm
Cuvettes utilizable on Cobas-Mira®
BSA84Multicells Segments in Methacrylate, individdualy wrapped.H 4,10 L 13,10 cm
BSA85Multicells Segments in Methacrylate, in rack. Box 30 racks of 15 pcs.H 4,60 L 18,50 D 11,10 cm
BSA024Microtube in PE Utilizable on Cobas® , 5 colors available.H 3,50 Ø 0,38 cm
Cobas® and Integra® are registered trade marks by ROCHE DIAGNOSTICS
Ref. Color.
BSA024 neutral
BSA024/BLU blue
BSA024/G yellow
BSA024/RA pink
BSA024/V green
BSA82Cover tray reagents utilizable on Cobas Mira® medium- 10 ml.H 39,5 Ø 29,5 mm
BSP30012 cells cuvette in Methacrylate with 2 pliers. Box 30 pcs. The plier holds 10 cuvettes.It’s advisable to transfer the cuvettes with the provided plierCuvette : H 2,00 L 5,20 D 0,86 cm
12 Cells Cuvettes
BIOSIGMADisposable Labware for Life Science
14
BSA031Sample cup utilizable on HITACHI® Vol. 3ml in polystyrene.H 3,81 Ø1,70 (max) 1,21 cm
BSA094Cuvettes utilizable on HITACHI® 902 in Bionex.L 11,20 H 4,60 D 4,41 cm
Cuvettes utilizable on Hitachi®
BSA096Cuvettes utilizable on HITACHI® 717/914 in Methacrylate.L 16,50 H 4,10 D 4,80 cm
BSA86Pediatric sample cup utilizable on Hitachi®, Elecsys®, Cobas Integra® and Core®. H 3,72 Ø 0,90, 1,69(max), 1,23 cm
HITACHI® is a registered trade mark by HITACHI JAPAN
BSA098Cuvettes utilizable on HITACHI® 911/912 in Bionex.L 16,70 H 4,04 D 4,70 cm
BSA107Single cuvette in Methacrylate. Interchangeable with BSA096 and BSA099. Rack of 200 pcs.H 2,80 L 0,79 D 0,62 cm BSA108Single cuvette in Bionex. Interchangeable with BSA094 and BSA098. Rack of 200 pcs.H 2,80 L 0,79 D 0,62 cm
BSA099Cuvettes utilizable on HITACHI® 704 in Methacrylate.L 11,60 H 3,80 D 2,90 cm
BIOSIGMADisposable Labware for Life Science
15
Cuvettes utilizable on Express®
ExPRESS® is a registered trade marks by Bayer
BSA658CEx sample cup utilizable on express® 550 and plus in polystyrene. H 1,91 ø 1,25 cm
BSA659CEx pediatric cup utilizable on express® 550 and plus in polystyrene. H 1,91 Ø 1,25 cm
BSA656Cartridge 13 cuvettes. Optic multisample cuvettes utilizable on express® 550 and plus + 24 bags.
BSA636Sample Nest Cup Vol.1 ml reduction cups, Ø 13 mm.
H 2,97 Ø 0,94 (max Ø 1,38) cm
Nesting sample cup
BSA646Sample Nest Cup Vol.2 ml reduction cups, Ø 16 mm.
H 2,97 Ø 1,24 (max 1,67) cm
Test tubes’ reducers are utilised when there is a small volume of serum so to allow tube rising and avoid that machine’s needle has difficulty to capture the serum.
BSA001MACRO CUVETTE utilizable on SPECTROPHOTOMETER in PS. Vol. 4 ml, box 100 pcs.
H 4,40 L 1,25 cm
BSA002SEMIMICRO CUVETTE utilizable on SPECTROPHOTOMETER in PS. Vol. 2 ml, box 100 pcs.
H 4,42 L 1,23 cm
Cuvettes utilizable on spectrophotometer
BIOSIGMADisposable Labware for Life Science
16
BSA651Sample Cup in PS utilizable on Olympus AU400/AU640.
H 4,90 Ø 1,37 (max Ø 1,54) cm
BSA046Sample Cup utilizable on Axsym in poly-styrene.
H 5,30 Ø 1,74 cm
BSA045TECHNICON Vol. 4 ml in polistirolo.Sample Cup utilizable on TECHNICON Vol. 4 ml in polystyrene.
H 3,78 Ø 1,53 cm
BSA030Sample Cup utilizable on GENSEAC Vol. 0,5 ml in polystyrene.
H 2,43 Ø 1,36 cm
BSA029Sample Cup utilizable on CENTRIFICHEM Vol. 0,25 ml in polystyrene.
H 1,62 Ø 1,36 cm
Sample and Coagulation Cups for clinical chemistry
BSA027Sample Cup utilizable on TECHNICON Vol. 1,5 ml in polystyrene.H 2,30 Ø 1,36 cm
BSA032Cuvette utilizable on FIBRINTIMER with metallic mixers aside, in polystyrene.
H 3,45 Ø 1,16 cm
BSA73Coagulation Cup utilizable on Option, Benk, Turbotimer. In PP.
H 2,24 Ø 0,86 cm
BSA731Coagulation Cup utilizable on Option, Benk, Turbotimer. In PP. With Metallic Mixers aside.
H 2,24 Ø 0,86 cm
BSA74Coagulation Cup with Metallic Steel Stick aside, utilizable on ACCU COA DATA. In PS.
H 3,51 Ø 0,86 cm
BSA77Coagulation Cup utilizable on SEAC CLOT 2 + steal stick. In PS.
H 3,47 Ø 1,34 cm
BSA5258Coagulation sample cups with optagonal top profile. In PS.
H 2,90 Ø 0,90 cm.
BSA102Coagulation Cup utilizable on Coag-Mat in PS.
H 2,34 Ø 1,00 cm
BSA69Sample Cup in PP utilizable on Biotec-nica Apparatus.
H 2,00 Ø 1,08 cm
BIOSIGMADisposable Labware for Life Science
17
Disposable slide for cell countingFast-Read 102®
The advantage of employing Fast-Read 102® is the ease in determinating the cells per µl in the specimen. The samples prepared by other methods typically contain various elements, which are often extremely difficult to determine for the presence of epithelial cells.With the use of the M-T System you get a smaller number of epithelial cells present in each field, reducing thus the possibility of overlap with other cel-ls, this system, which ensures a result more accurate and precise, facilitate to the technicians the determination of the presence of cellular elements.After examining, under a microscope, the area of Fast-Read 102® with a lower magnification, it will pass to an higher magnification to analyze each cellular element accordingly .
How to count the cellsAfter microscope reading, to quantify cells on the small grid ( 1 mm x 1 mm, volume 0,1 μl ). For no. of cells/UL computation, it must observ example here below where it has been counted 3 cells in 1 mm² of grid:
no. cells/µ.= ___________________________________________ x 10 ( Vol. to obtain 1µ) = 3 cells/µl3 (number of cells counted) 10 (concentration factor)
Concentration factor = 10 :Because we are reading concentrated urine from 10 ml to 1 ml
VOLUME = 10 :Because the grid has dimension 1 mm x 1 mm, depth 0.1 mm and the internal volume to this grid is 0.1 μl
Cell volume 7 µl
Grid dimensions 5 x 2 mm
Depth 0,1 mm
Grid volume 1 µl
Each grid includes squares 1 x 1 mm
Square volume 0,1 µl
Channel vent capacity 30 µl
Chamber total volume 37 µl
Material PMMA
Box 100 pz. x cf.
It’s very important matter during urine sedimentation count, that operators always use the same sample preparation method in order to have tests’ repeatibility. Furthermore, with traditional urine reading system actually in use in many laboratories, sedimentation compaction creates a superimposition of epithelial cells. That cause difficulties in verifying the presence amount of different cells components. With Biosigma patented M-T System it is possible to overcome this inconveniences in a few standardisation steps during sample preparation. Here steps as follows:
Accessories
M-T System
1) Place BAP074 tests tubes, contai-ning urine to be analysed, into express rack BSL130 and then centrifuge.
2) Insert tribulb M-T Microtips BSV143 making sure to trap the tubes
3) Overturn the rack to come out the supernatant
BSV143M-T Microtips®: a special calibration and design that allows you to block 1 ml of urine sediment on the bottom tube. With this pipette you’ll be able to standardize the urinary sediment in 1ml, creating the right proportion between 10 ml of urine collected in a tube and 1ml of urine sediment that remains stuck in the bottom of the tube.
BSP074Tube for M-T System 16 x 103 mm in transparent polystyrene, graduated to 1 / 2,5 / 5 / 10 ml, utilizable with M-T Microtips®.
BSP060 idem BSP074 in PP
1 2 3
4) You get 1 ml of sediment re-suspended ready to be analysed on Fast Read 102® Slides
4
BIOSIGMADisposable Labware for Life Science
18
BSTA18Test tubes with granulars separators and coagulation accelerator, rack of 50 units, white stopper Vol. 10 ml, ø 16 x 100 mm, in pp.
BSTA19 Idem BSTA18 Labelled.
Sorwall test-tubes 12x75mm BSA059Vol. 5ml, Ø 12x75 mm in polystyrene, cylindrical. Without rim.
BSA059/PVol. 5ml, Ø.12 x 75 mm in polypropylene, cylindrical. Without rim.
BSA0594/PVol. 6ml, Ø 13x75 mm In polypropylene, cylindrical. Without rim.
Sorwall test-tubes 13x75mm
BSA0592Blue Sorwall test-tube in PS. Without rim.
BSA0594Vol. 6ml, Ø 13x75 mm In polystyrene, cylindrical. Without rim.
Polyetylene stoppersColor Ø 12 mm COD. Ø 16 mm COD.
red BSO036 BSO025
white BSO037 BSO026
yellow BSO038 BSO027
dark green BSO039 BSO028
light blue BSO040 BSO029
blue BSO041 BSO030
pink BSO043 BSO032
Color Ø 11/12mm COD. Ø 16/17 mm COD.
neutral BSA062 BSA063
light blue BSA062/AZ BSA063/AZ
white BSA062/B BSA063/B
blue BSA062/BLU BSA063/BLU
yellow BSA062/G BSA063/G
pink BSA062/RA BSA063/RA
red BSA062/RO BSA063/RO
green BSA062/V BSA063/V
Polyetylene stoppers with wings
BSA026 BSA037
BSA063
BSP058Cylindrical Vol. 10 ml, Ø 16 x 100 mm grad.: 2,5 / 5 /10 ml Polypropylene
BSP072Cylindrical Vol. 10 ml, Ø 16 x 100 mm grad.: 2,5 / 5 /10 ml Polystyrene
BSA062
BSO100Stopper with security holder for Sorwall tubes.
Ref. Color
BSO100 white
BSO100/BLU blue
BSO100/V green
BSO100/GR grey
BSO100/RO red
BIOSIGMADisposable Labware for Life Science
19
Disposable loops 1ul, sterile, in polypropylene. One side is provided of loop head and the other side of inoculating needle. The easy pell-pack open pouch enables fast and easy access to the product especially when wearing latex gloves). Peel pack shows lot no and sterility expiry date. Peel pack can be of 5, 10, 20 units or individually wrapped.
BSM023L-Shaped Plastic Spreaders 90° angle, in white polystyrene, in sterile pouches of 5 units.
Ref. Box Color
BSV121 peel pack of 20 pcs neutral
BSV1211 individually wraped neutral
BSV1215 peel pack of 5 pcs neutral
BSV1210 peel pack of 10 pcs neutral
Ref. Box Color
BSV120 peel pack of 20 pcs blue
BSV1201 individually wraped blue
BSV1205 peel pack of 5 pcs blue
BSV1200 peel pack of 10 pcs blue
Disposable loops 10ul, sterile, in polypropylene. One side is provided of loop head and the other side of inoculating needle. The easy pell-pack open pouch enables fast and easy access to the product especially when wearing latex gloves). Peel pack shows lot no and sterility expiry date. Peel pack can be of 5, 10, 20 units or individually wrapped.
10 µl
1 µl
Inoculation loops
BSM0231L-Shaped Plastic Spreaders 90° angle, in white polystyrene, in sterile pouches of single units
Loops 10 µl
Loops 1 µl
Vacustopper™BSO113Vacustopper in polyethylene Ø 13 mm for vacuum tubes reclosing after processing or for standard Ø 12 and 13 mm tunes. His specific internal shape allows the cap to internally join the tube and to grant a seconf external closing thanks to the soft polyethylene.Uses:1: Freezing tubes in a refrigerator after processing2: Safety shipment of processed samplesSuitable with vacuum tubes: BD Vacutainer®, Venosafe® Terumo, Vacuette ® Greiner-Bio One.
BIOSIGMADisposable Labware for Life Science
20
Filter tips Microguard e Multiguard MµlTi (series)They offer two kinds of surface:
Ò The surface of the “standard” virgin polypropylene; Ò The inner surface “Low Binding”: the tip, made hydrophobic, reduces
the surface tension. This property has two benefits: a refund of the sample and a maximal retention of DNA and proteins, much weaker than a standard tip.
In rack with lid In rack with zip-lidStandard Low Binding Standard Low Binding
Model Volume Features Compatibility Ref. Units / Box Ref. Units / Box Ref. Units / Box Ref. Units / Box
Microguard10F
0,1-10 µl Small volumes tips
Finnpipette 2-10 µl - - - - - - DD028300T 10x96
Microguard10G
0,1-10 µl Small volumes micro tips
Gilson P2-P10,Finnpipette 2-10 µl
Biohit 2,5-10 µl
DD028200 10x96 DD035200 10x96 - - - -
MIcroReach Guard
0,1-10 µl Universal long (45 mm)
tips
Gilson P2-P10 Eppendorf 10 µl
Finnpipette 2-10 µlBiohit 2,5-10 µl
DD038000 10x96 DD038000T 10x96 - - DD030360T 10x96
Microguard 10xL
0,1-10 µl Universal long (45 mm)
tips
Gilson P2-P10 Eppendorf 2,5 µl – 10 µl
Finnpipette 2-10 µlBiohit 2,5-10 µl
- - - - DD130340 10x96 - -
Multiguard10E
0,5-10 µl Crystal long tips
Eppendorf 10 µlSocorex 10 µl
DD015020 10x96 DD035210 10x96 - - - -
Multiguard 20 µl
5–20 µl Universal Gilson P20Eppendorf 20 µl
Finnpipette 10-20 µlSocorex 20 µl
DD014210 10x96 DD035220 10x96 - - DD030370T 10x96
Multiguard 100 µl
5-100 µl Universal Gilson P100Biohit 50-100 µlEppendorf 100 µl
Finnpipette 50-100 µlSocorex 100 µl
DD036060 10x96 DD036060T 10x96 - - DD030380T 10x96
Multiguard 200 µl
5-200 µl Universal Gilson P200Eppendorf 200 µlFinnpipette 200 µl
Socorex 200 µl
DD014220 10x96 DD035230 10x96 - - DD030390T 10x96
MultiGuard3 e 200 µl
20, 100 e 200 µl
Long tips Gilson P20 – P100 – P200Biohit 50-100 µl
Eppendorf 20-100-200 µlFinnpipette
10-20-50-100-200 µl
DD017370 10x96 DD035240 10x96 - - - -
Multiguard 300 xT
5-300 µl Long tips 78 mm
Gilson P200Biohit 250 µl
Eppendorf 200 µlFinnpipette 200 µl
DD130510 10x96 DD130510T 10x96 - - - -
Multiguard 1000 µl
100-1000 µl
Universal Gilson P1000Eppendorf 1000 µlFinnpipette 1000 µl
Biohit 1000 µlSocorex 1000 µl
DD014200 10x100 DD035260 10x96 - - - -
Multiguard 1250 µl
100-1250 µl
Long tips 102 mm
For pipette of1000 µl
- - - - DD134000 8x96 - -
“Low binding” tip allows sample’s total
expulsion
Residual sample with classic tips
BIOSIGMADisposable Labware for Life Science
21
MµlTi tips for automationTips for automated pipetting stations for Beckman, Tecan and Zymark.
Ò Certified RNase, DNase and endotoxin free Ò In rack Ò With or without filter
Black Tips for Tecan Light Tips for Zymark Light Tips 1000 and 2000
for Beckman Biomek
Black Tips for beckman SPAN-8
Light Tips for Beckman FX
Volume µl Sterile Filter Ref. Units / Box Ref. Units / Box Ref. Units / Box Ref. Units / Box Ref. Units / Box
10 No No - - - - - - - - DD111234 4800
10 Yes No - - - - - - - - DD111235 4800
10 Yes Yes - - - - - - - - DD111236 4800
20 No No DD111201 2304 - - DD111223 4800 DD111246 4800 DD111237 4800
20 Yes No DD111200 2304 - - DD111224 4800 DD111247 4800 DD111238 4800
20 Yes Yes DD111202 2304 - - DD111225 4800 DD111248 4800 DD111239 4800
30 Yes Yes - - - - - - DD111251 4800 DD111242 4800
50 No No DD111204 2304 DD111214 4800 DD111226 4800 DD111249 4800 DD111240 4800
50 Yes No DD111203 2304 DD111215 4800 DD111227 4800 DD111250 4800 DD111241 4800
50 Yes Yes DD111205 2304 DD111216 4800 - - - - - -
100 No No - - DD111217 4800 - - - - - -
100 Yes No - - DD111218 4800 - - - - - -
100 Yes Yes - - DD111219 4800 - - - - - -
165 Yes Yes - - - - DD111230 4800 DD111254 4800 DD111245 4800
170 Yes Yes - - DD111222 4800 - - - - - -
200 No No DD111207 2304 DD111220 4800 - - - - - -
200 No No DD111208 6912 - - - - - - - -
200 Yes No DD111206 2304 DD111221 4800 - - - - - -
200 Yes Yes DD111209 2304 - - - - - - - -
250 No No - - - - DD111228 4800 DD111252 4800 DD111243 4800
250 Yes No - - - - DD111229 4800 DD111253 4800 DD111244 4800
1000 No No DD111211 1536 - - DD111231 360 - - - -
1000 No No DD111212 4608 - - - - - - - -
1000 Yes No DD111210 1536 - - DD111232 360 - - - -
1000 Yes Yes DD111213 1536 - - DD111233 360 - - - -
BIOSIGMADisposable Labware for Life Science
22
MµlTi tips RNase and DNase-free. Exceptional precision and reproducibility. An incomparable choice.
Tips in bulkTips in Cell rack usable also with
Quick Rack
Tips in zip-lid rack usable also with
Quick RackTips in QuickRack
Description Type Sterile Ref. Units / Box
Ref. Units / Box
Ref. Units / Box Ref. Units / Box
Tip 0,1 - 10 µl Micro-Guard 10xL long graduated 1, 2, 5 ,10 µl Universal
Standard No - - - - DD030560A 10 x 96 - -
Standard No - - - - - - - -
Standard Yes - - - - DD030570A 10 x 96 - -
Low-binding No - - - - - - - -
Low-binding Yes - - - - - - - -
Tips 0,5 - 10 µl crystal for
Eppendorf E-tip
Standard No DD013970 1000 DD027510 10 x 96 - - - -
Standard No DD013980 10000 - - - - - -
Standard Yes - - DD027500 10 x 96 - - - -
Low-binding No DD035040A 1000 DD135060 10 x 96 - - - -
Low-binding Yes - - DD135070 10 x 96 - - - -
Tip 0,1 - 10 µl micro for Gilson G
MicroTip
Standard No DD023580A 1000 DD023560A 10 x 96 - - DD030470 5 x 960
Standard No DD023580 10000 - - - - - -
Standard Yes - - DD023570A 10 x 96 - - - -
Low-binding No DD035000A 1000 DD035010A 10 x 96 - - DD035030 5 x 960
Low-binding Yes - - DD035020A 10 x 96 - - - -
Tips 0,1 - 10 µl MicroReach long,
Universal
Standard No DD037650A 1000 DD037640A 10 x 96 DD026400A 10 x 96 DD037820 5 x 960
Standard No DD037650 10000 - - - - - -
Standard Yes - - DD037660A 10 x 96 DD026410A 10 x 96 - -
Low-binding No DD037650TA 1000 DD037640TA 10 x 96 DD026400TA 10 x 96 DD037820T 5 x 960
Low-binding Yes - - DD037660TA 10 x 96 DD026410TA 10 x 96 - -
Tip 1 - 200 µl Soft Tip Universal, gra-
duated, yellow
Standard No DD032430A 1000 DD032240 10 x 96 - - DD030440 5 x 960
Standard No DD015730 10000 - - - - - -
Standard Yes - - DD032260 10 x 96 - - - -
Tip 1 - 200 µl Soft Tip Universal, gra-
duated, neutral
Standard No DD010440 1000 DD032230 10 x 96 DD026420A 10 x 96 DD030430 5 x 960
Standard No DD015720 10000 - - - - - -
Standard Yes - - DD032250 10 x 96 DD026430A 10 x 96 - -
Low-binding No DD035080A 1000 DD135090 10 x 96 DD026420TA 10 x 96 DD035110 5 x 960
Low-binding No DD035080 10000 - - - - - -
Low-binding Yes - - DD035100A 10 x 96 DD026430TA 10 x 96 - -
Tip 1 - 300 µl extra long,
graduated, neutral
Standard No DD130800A 1000 DD130920 10 x 96 - - - -
Standard Yes - - DD130930 10 x 96 - - - -
Low-binding No DD130800TA 1000 DD130920T 10 x 96 - -
Low-binding Yes - - DD130930T 10 x 96 - - - -
Tip 100 - 1000 µl Universal, non
graduated, blue
Standard No DD010020 1000 DD027110 10 x 100 - - - -
Standard No DD010040 10000 - - - - - -
Standard Yes - - DD027120 10 x 100 - - - -
Tip 100 - 1000 µl Universal,
non graduated, neutral
Standard No DD010180 1000 DD010200A 10 x 100 - - - -
Standard No DD010190 10000 - - - - - -
Standard Yes - - DD010230A 10 x 100 - - - -
Low-binding No DD035170A 1000 DD035180A 10 x 100 - - - -
Low-binding No DD035170 10000 - - - - - -
Low-binding Yes - - DD035190A 10 x 100 - - - -
Tip 50 - 1250 µl Universal,
graduated, neutral
Standard No DD031610A 768 - - DD134750 8 x 96 DD134770 5 x 768
Standard No DD031610 10 x 768 - - - - - -
Standard Yes - - - - DD134760 8 x 96 - -
BIOSIGMADisposable Labware for Life Science
23
Tips on PLATINUM refills
on tray (without rack)
Starter Kit Tips in TipStation
Tips in zip-lid rack
usable also with QuickShot
Tips on QuickShot
Description Type Sterile Ref. Units / Box Ref. Units / Box Ref. Units / Box Ref. Units / Box Ref. Units / Box
Tip 0,1 - 10 µl Micro-Guard 10xL long graduated 1, 2, 5 ,10 µl Universal
Standard No - - - - - - - - - -
Standard No - - - - - - - - - -
Standard Yes - - - - - - - - - -
Low-binding No - - - - - - - - - -
Low-binding Yes - - - - - - - - - -
Tips 0,5 - 10 µl crystal for
Eppendorf E-tip
Standard No - - - - - - - - - -
Standard No - - - - - - - - - -
Standard Yes - - - - - - - - - -
Low-binding No - - - - - - - - - -
Low-binding Yes - - - - - - - - - -
Tip 0,1 - 10 µl micro for Gilson G
MicroTip
Standard No - - - - - - - - - -
Standard No - - - - - - - - - -
Standard Yes - - - - - - - - - -
Low-binding No - - - - - - - - - -
Low-binding Yes - - - - - - - - - -
Tips 0,1 - 10 µl MicroReach long,
Universal
Standard No DD170500 4 x 960 DD170700 10 x 96 DD027780 5 x 960 - - - -
Standard No - - - - - - - - - -
Standard Yes - - - - DD027790 5 x 960 - - - -
Low-binding No DD170600 4 x 960 - - DD027780T 5 x 960 - - - -
Low-binding Yes - - - - DD027790T 5 x 960 - - - -
Tip 1 - 200 µl Soft Tip Universal, gra-
duated, yellow
Standard No DD170520 4 x 960 DD170720 10 x 96 DD027720 5 x 960 DD032000A 10 x 96 DD032610 10 x 960
Standard No - - - - - - - - - -
Standard Yes - - - DD027730 5 x 960 DD032100A 10 x 96 DD032390 1
Tip 1 - 200 µl Soft Tip Universal, gra-
duated, neutral
Standard No DD170510 4 x 960 DD170710 10 x 96 DD027760 5 x 960 DD032270 10 x 96 DD032600 10 x 960
Standard No - - - - - - - - - -
Standard Yes - - - DD027770 5 x 960 DD032280 10 x 96 DD032380 1
Low-binding No DD170610 4 x 960 - - DD027760T 5 x 960 DD031800TA 10 x 96 DD035120 10 x 960
Low-binding No - - - - - - - - - -
Low-binding Yes - - - - - - DD031900TA 10 x 96 - -
Tip 1 - 300 µl extra long,
graduated, neutral
Standard No DD170530 4 x 960 DD170730 10 x 96 - - - - - -
Standard Yes - - - - - - - - - -
Low-binding No DD170620 4 x 960 - - - - - - - -
Low-binding Yes - - - - - - - - - -
Tip 100 - 1000 µl Universal, non
graduated, blue
Standard No - - - - - - - - - -
Standard No - - - - - - - - - -
Standard Yes - - - - - - - - - -
Tip 100 - 1000 µl Universal,
non graduated, neutral
Standard No - - - - - - - - - -
Standard No - - - - - - - - - -
Standard Yes - - - - - - - - - -
Low-binding No - - - - - - - - - -
Low-binding No - - - - - - - - - -
Low-binding Yes - - - - - - - - - -
Tip 50 - 1250 µl Universal,
graduated, neutral
Standard No DD170540 4 x 768 DD170740 8 x 96 - - - - - -
Standard No - - - - - - - - - -
Standard Yes - - - - - - - - - -
MµlTi tips RNase and DNase-free. Exceptional precision and reproducibility. An incomparable choice.
BIOSIGMADisposable Labware for Life Science
24
Tips One Touch® MµlTi are the bigger innovation in the handling of liquid samples.
Ò Innovative production process: bi-component tips Ò Body of tip is on virgin traditional polypropylene Ò The Upper part is in flexible elastomer Ò It is optimally suited for most pipettes Ò Optimal water resistance and accuracy Ò They need a little pressure for the inclusion and for the tip ejection Ò Greatly reduce the muscle fatigue of the operator Ò They can be used in plastic racks or in cardboard E-DEK-bio-degradable racks Ò Guarantee an optimal load in terms of use with multichannel pipettes Ò A particular resistance of cardboard racks without these being crushed during the use. Ò The One Touch without filter tips in racks are autoclavable Ò The revolutionary packaging: cardboard racks E.DEK reduces quantity of the plastic waste
Rack in Plastic Rack in Carton E.DEK
Tips Ref. Units / Box Ref. Units / Box
10/20 µl non sterile DD010300 10 x 96 - -
10/20 µl sterile DD010310 10 x 96 DD010390 50 x 96
10/20 µl with Filter, sterile DD010320 10 x 96 DD010400 10 x 96
100 µl with Filter, sterile DD010510 10 x 96 DD010530 10 x 96
300 µl non sterile DD010330 10 x 96 - -
300 µl sterile DD010340 10 x 96 DD010410 50 x 96
300 µl with Filter, sterile DD010350 10 x 96 DD010420 10 x 96
1000 µl non sterile DD010360 8 x 96 - -
1000 µl sterile DD010370 8 x 96 - -
1000 µl with Filter, sterile DD010380 8 x 96 DD010460 12 x 64
Tips One Touch® MµlTi bi-component
Pend
ing
Pate
nt
Empty racks Ref. Description Units / BoxDD028360 Rack for tips 0,1 - 10 µl G Micro Tip 10
DD028310 Rack “Cell Rack” for tips 200 µl and 10 µl Micro-Reach
10
DD028320 Zip-lid rack for tips 200 µl (for QuickShot) 10
DD021750 Zip-lid rack for tips 1250 µl 8
DD027280 Zip-lid rack for tips 200 µl and 10 µl MicroReach 10
Ref. Description Units / BoxDD071000 Rack for tips 10, 200, 300 µl 1
DD371000 Rack for tips 10, 200, 300 µl 24
DD071010 Rack for tips 1250 µl 1
DD371010 Rack for tips 1250 µl 16
Rack for PLATINUM tips
BIOSIGMADisposable Labware for Life Science
25
Capillary tips Mµltiflex and Miniflex for gelCapillary tips to deposit the gel with high precision.
Ò The capillary tips with round tip (0,57 mm O.D.) are used especially for SDS PAGE gel. Ò The capillary tip with flat spatula tip (0,17 e 0,37 mm O.D.) are indicated for sequencing gel. The big length of tips 200
µl (83 mm) allow use on deep tubes. Ò Certified RNase, DNase and pyrogenic free. Ò In rack of 200 pcs., autoclavables and with lid.
200-tip rack non sterile 200-tip rack sterileVolume
µl Tip shape Ø Ext.
(mm)Length (mm)
Ref. Units / Box Ref. Units / Box
For all micropipettes of 200 µl
0,5 - 200 Round 0,57 83,5 DD021570 2 x 200 DD013810 4 x 200
0,5 - 200 Flat Spatula 0,37 83,5 DD027600 2 x 200 DD013770 4 x 200
0,5 - 200 Flat Spatula 0,17 83,5 DD017310 4 x 200 DD017320 4 x 200
For micropipettes 10 µlGilson, Biohit, Labsy-stems,
0,1 - 10 Round 0,57 83,5 DD015100 4 x 200 DD015110 4 x 200
0,1 - 10 Flat Spatula 0,37 83,5 DD015120 4 x 200 DD015130 4 x 200
0,1 - 10 Flat Spatula 0,17 83,5 DD017350 4 x 200 DD017360 4 x 200
For micropipettes 10 µl Eppendorf
0,1 - 10 Round 0,57 83,5 DD013920 4 x 200 DD013930 4 x 200
0,1 - 10 Flat Spatula 0,37 83,5 DD013890 4 x 200 DD013900 4 x 200
0,1 - 10 Flat Spatula 0,17 83,5 DD017330 4 x 200 DD017340 4 x 200
Capillary tips Mµltiflex 0,5 – 200 µl - in bulk
- - - - DD028480A 1000 - -
BIOSIGMADisposable Labware for Life Science
26
Certifications
Biosigma is UNI EN ISO 13485:2004, Quality Management Sys-tem.
Biosigma’s products are also in accordance with the Directive 98/79 CE referred to in vitro diagnostic medi-cal devices.
Dispositivi Medico Diagnostici In Vitro.According to the Directive 98/79/EC.
“Keep dry”.
“Do not reuse”. “Batch code”.
“Consult Instructions for Use”. “Use by”.
“Temperature limitation”. “Manufacturer”.
“In Vitro Diagnostic Medical Device”. “Fragile, handle with care”.
“Method of sterilization using irradia-tion”.
“Method of sterilization using ethylene oxide”.
“Do not use if package damaged”. “Keep away from heat”.
“Date of manufacture”. “Attention, see instructions for use”.
Interpretation of the symbols on the package