development and commercialization for bio pesticide by using pseudomonas
TRANSCRIPT
DEVELOPMENT AND COMMERCIALIZATION FOR BIO-PESTICIDE BY USING PSEUDOMONAS
FLUORESCENCE
INTRODUCTION P. fluorescence is gram negative bacillus
These bacteria are easy to culture, as they grow on minimal media and at 25-30 degrees Celsius
They destroy certain toxins and pollutants including styrene, polycyclic aromatic hydrocarbons, and TNT
They also protect plants from infection by pathogens by producing secondary metabolites like antibiotics and hydrogen cyanide which kill other bacteria and fungi
ABSTRACT In physical characteristic test in various media P. fluorescence shows positive growth
In biochemical characteristic on various media P. fluorescence shows positive result
In anti-pathogenic activity test of P. fluorescence also shows positive result against F. oxysporum. This test confirm that P. fluorescence can be use as bio-fertilizer
MATERIAL AND METHOD All the pure culture of P. fluorescence are taken from DESIGN
BIOSIS
All chemical & media were prepared to evaluate the biochemical assay, physical appearance & anti-pathogenic activity test of P. fluorescence
Also microscopic observation of P. fluorescence was done
Physical activity test
various media was used to determine the physical appearance of P. fluorescenceThose are King’s B agar media King’s Broth media Starch agar and starch broth media LB Broth media pikovaskaya's agar and broth media
Biochemical activity test
various media was use to determine the Biochemical activity test for P. fluorescenceThose are Citrate agar slant media Starch agar media Czapek agar medium
Anti-pathogenic activity test
various media was use to determine the anti-pathogenic activity test for P. fluorescenceThose are LB agar media Potato dextrose agar medium
Physical characteristic photos
King’s B agar medium
King’s B Broth media LB Broth media
Starch Broth media Starch agar media
Pikovaskaya's agar media Pikovaskaya's agar media
Biochemical activity test photos
Citrate agar medium
Starch agar medium
Czapek agar medium
Anti-pathogenic activity testIn PDA media F.oxysporum
P. fluorescence
Mat based inhibition of F.oxysporium(Plant Pathogen)
In PDA media
F. oxysporum P. fluorescence F.oxysporum
Inoculum based inhibition of F.oxysporium (Plant -Pathogen)
In LB agar mediaF. oxysporum
P. fluorescence
F. oxysporium is inoculated in middle and P. fluorescence Inoculated in around that F. oxysporium(Plant pathogen)
In LB agar media
F. oxysporum
P. fluorescence
Mat based inhibition of F.oxysporum (Plant Pathogen)
Mass Culture, Bio-formulation and commercialization
Blander
Mass culture ofPseudomonas fluorescence
Market product
RESULTPhysical activity test
From the above photos we can observed that in starch agar media the growth of Pseudomonas fluorescence is greater than Kings B media, pikovaskaya's media.
In fact in pikovaskaya's agar slant media was to less.
Biochemical activity test The biochemical assay confirmed that Pseudomonas
fluorescence has citrate utilizing, starch hydrolysis and cellulose activity.
This experiment shows the proof and practical identification
and confirmation method for P. fluorescence.
Anti-pathogenic activity test The anti-pathogenic activity of P. fluorescence against F.
oxysporum shows that the mat based formulation of bio-fertilizer is effective against F. oxysporum rather than liquid based preparation.
Also P. fluorescence can be added in combination with other soil friendly microorganisms to inhibit of the plant pathogens including F. oxysporum in it’s soil based application
Mass Culture, Bio-formulation and commercialization
The bio-formulation of P. fluorescence is an easy way if can be done by P. fluorescence Mat mixing with Talc powder and CMC.
The commercialization of this bio-formulation can be widely used as the plant pathogen eradication or control by using natural resources are very few in Agriculture sector.
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