8/12/2019 Diabetic Disturbances

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Correlative and quantitative 1H NMR-based metabolomics

reveals specific metabolic pathway disturbances in diabetic rats

Shucha Zhanga, G.A. Nagana Gowdaa, Vincent Asiagoa, Narasimhamurthy Shanaiaha,

Coral Barbasb,*, and Daniel Rafterya,*

aDepartment of Chemistry, Purdue University, West Lafayette, IN 47907, USA

bSan PabloCEU University, Boadilla del Monte, 28668 Madrid, Spain

Abstract

Type 1 diabetes was induced in SpragueDawley rats using streptozotocin. Rat urine samples (8

diabetic and 10 control) were analyzed by 1H nuclear magnetic resonance (NMR) spectroscopy.

The derived metabolites using univariate and multivariate statistical analysis were subjected to

correlative analysis. Plasma metabolites were measured by a series of bioassays. A total of 17urinary metabolites were identified in the 1H NMR spectra and the loadings plots after principal

components analysis. Diabetic rats showed significantly increased levels of glucose (P< 0.00001),

alanine (P< 0.0002), lactate (P< 0.05), ethanol (P< 0.05), acetate (P< 0.05), and fumarate (P