Based on nucleic acid amplification, Speed-oligo® presents all the advantages of molecular biology in quick detection for infectious diseases, providing immediate, highly sensitive and specific results.

New product design for an affordable, automated and contamination free protocol

Vircell S.L. | info@vircell.com | Tel. +34 958 441 264 | www.vircell.com

Diagnostic solutions for human infectious diseases

New

The new Speed-oligo® product line adapts its presentation to enlarge its diagnostic possibilities, simplifies test procedure and allows for automation.

Speed-oligo® allows for visual and direct analysis, through a simple technique which provides results in just a few minutes. This PCR-based method coupled to a dipstick device enables a rapid, highly sensitive and specific detection.

The Speed-oligo® cassette contains lyophilized PCR mix and a disposable cassette including test strip. The user just needs to resuspend the prepared PCR mix and add the clinical sample.

The new design is a closed, self-contained cassette allowing for a contamination-free protocol.

Fast test resultsHighly sensitive and specific detection - Double hybridizationEasy to use - No handling of toxic substancesPrepared PCR mix prevents contaminationLyophilized presentation - Transport costs savings CE marked (IVD)

MAIN FEATURES

Avoids risk of contaminationImproves the possibilities of automation increasing sample throughput Simplifies the assay protocol (single pipetting for sample addition)Allows multiplex testing- more test lines can be includedImproves sensitivity

MAIN IMPROVEMENTS

Cat. No. Product description Pack size

SPC004 24 testsSPEED-OLIGO® MYCOBACTERIA CASSETTEDetection of Mycobacterium genus and the 19 most frequently isolated species in culture samples: M. tuberculosis complex, M. abscessus, M. avium, M. chelonae, M. fortuitum, M. gordonae, M. interjectum, M.intracellulare, M. kansasii, M. malmoense, M. marinum/M. ulcerans, M. peregrinum, M. scrofulaceum, M. xenopi (11 test lines)

SPC001 24 testsSPEED-OLIGO® DIRECT MYCOBACTERIUM TUBERCULOSIS CASSETTEDirect detection of Mycobacterium genus and M. tuberculosis complexin clinical samples (2 test lines)

SPC003 24 testsSPEED-OLIGO® BACTERIAL MENINGITIS CASSETTEDetection of Streptococcus pneumoniae, Haemophilus influenzae and Neisseria meningitidis (3 test lines)

SPC002 24 testsSPEED-OLIGO® BORDETELLA CASSETTEDetection of B. parapertussis, Bordetella sp. and B. pertussis (3 test lines)

SPC005 24 testsSPEED-OLIGO® BACTERIAL PNEUMONIA CASSETTEDetection of Bordetella sp., Chlamydophila pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae (4 test lines)

SPC006 24 testsSPEED-OLIGO® HUMAN PAPILOMAVIRUS CASSETTEIndividual detection of 14 high risk genotypes of HPV: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 (14 test lines)

1st HYBRIDIZATION

2nd HYBRIDIZATION

Avoids the detection of unspecific amplified fragments providing excellent test specificity

Double hybridization

Reaction of the specific and control amplicons with the complementary probes coupled to colloidal gold nanoparticles

The complexes of specific and control amplicons and the colloidal gold conjugates react with a second probe which is bound to the membrane, leading to a coloured band.The excess of conjugate reacts with a complementary probe on the control line.

Denatured and amplified DNA

PCR amplification control line

Product control lineTest zone

Parque Tecnológico de la Saludc/ Avicena 8, 18016 Granada, SpainT. +34 958 441 264 F. +34 958 510 712info@vircell.com www.vircell.com

Speed-oligo® Workstation is a compact and walk-away platform that carries out the entire post-amplification process

This new platform automates the detection, reading and interpretation of results based on Speed-oligo®, the oligochromatografic technique developed by Vircell.The new system can process up to 16 samples in each run obtaining results in 20 minutes after DNA amplification.

Hybridization area

Denaturation area

Scanner

Ultraviolet lamp

Starting point

Touch screen

After the amplification of the samples, vials and cassettes are loaded for the individual identification of samples by means of the touch screen.

Located in the robotic arm, the ultraviolet lamp ensures the routine maintenance of the platform and allows for additional cleaning in case of any incident.

PCR vials are exposed to a temperature that separates double stranded amplified nucleic acid.

In this area the cassettes will be preheated. Then the closed tubes containing the denatured PCR product will be introduced in the cassettes releasing the amplified product that will get in contact with the strips.

Speed-oligo® Workstation makes an initial scanning of the cassettes for the identification of the test loaded.At the end of the process the system scans the cassettes for the reading and interpretation of the results.

The access to the system is very simple and intuitive by means of a touch screen.At the beginning of the detection process, the user identifies samples and after the procedure is concluded, results will be available as individual reports.

• Capacity: 16 samples• Results in 20 minutes after the PCR• Medium-sized, adapts to any laboratory• Robust and consistent platform with excellent mechanical and thermical reliability• Individual introduction of sample data by means of a touch screen• Automatic checking for the correct placement of the samples• Reading and automatic interpretation of results• Compatible with most LIS systems• Convenient USB ports allow for exporting or printing sample result reports• Ability to perform different tests under the same hybridization protocol

MAIN FEATURES

Speed-oligo® Workstation (Ref. SPWS)