diversity of - biomarksbiomarks.eu/sites/default/files/wiebe-light.pdf · 2014-10-29 · diversity...

Diversity of Leptocylindrus and Tenuicylindrus in BioMarKs samples

Deepak Nanjappa (PhD student, Now PhD. SZN) Wiebe HCF Kooistra (SZN) Adriana Zingone (SZN) Stephane Audic (SBR) The whole BioMarKs team BioMarKs London Meeting

Questions: can nuclear SSU rDNA V4-sequences, obtained from BioMarKs samples, be used to

•  Detect species, and

•  Circumscribe the biodiversity

in the planktonic diatom genera Leptocylindrus and Tenuicylindrus?

Project BioMarKs Diversity of Leptocylindrus and Tenuicylindrus in BioMarKs samples

What are Leptocylindrus and Tenuicylindrus? •  Radial centric diatom genera

•  Simple morphology


Leptocylindrus danicus

Tenuicylindrus belgicus

Why explore biodiversity of Leptocylindrus and Tenuicylindrus

•  Generally at the base of the nuclear SSU rDNA tree, as

sister lineage to all the remainder of the diatoms,

•  Apparently species-poor “remnant”-lineage, but species diversity is poorly known,

•  Many ancestral morphological features,

•  The only diatom lineage that forms resting spores immediately following sexual reproduction,

•  Ecologically important and widespread.


The nuclear SSU rDNA tree


Achlya apiculataPhytophthora undulata

Developayella elegansPirsonia punctigeraHyphochytrium catenoides

Tetrasporopsis fuscescensGiraudyopsis stellifera

Antarctosaccion applanatumPleurochloridella botrydiopsis

Heterococcus pleurococcoidesBotrydiopsis constricta

Botrydiopsis alpinaMischococcus sphaerocephalus

Phaeothamnion confervicolaStichogloea doederleiniiChoristocarpus tenellusDiscosporangium mesarthrocarpum

Spatoglossum pacificumDictyota dichotoma

Bodanella lauterborniSporochnus comosusSaccharina japonica

Polypodochrysis teissieriPinguiochrysis pyriformis

Glossomastix chrysoplastaFibrocapsa japonica CCMP1661

Chattonella antiquaHaramonas viridis

Rhizochromulina marinaHelicopedinella tricostata

Pteridomonas danicaDictyocha fibula

Dictyocha speculumFlorenciella parvula

Verrucophora farcimenSarcinochrysis marina

Aureococcus anophagefferensPelagomonas calceolata

Pseudotetraedriella kamillaeVischeria helveticaEustigmatos magna

Synchroma grandeChrysochaete britannica

Chromophyton rosanoffiiChrysocapsa paludosa

Chrysonebula flavaHibberdia magna

Ochromonas danicaOchromonas tuberculataChrysolepidomonas dendrolepidota

Chromulina chionophilaChrysosphaera parvulaPhaeoplaca thallosaMallomonas adamasTessellaria volvocina CCMP1781

Mallomonas annulataSynura curtispina CCMP847

marine eukaryote NW414.14Uncultured marine diatom DH22_2A36

freshwater eukaryote LG22-09Uncultured stramenopile Bothnian Bay

Uncultured eukaryote DSGM-35Uncultured stramenopile 1-E8

Uncultured eukaryote A10Uncultured stramenopile 3b-A8

Marine eukaryote BLACKSEA_cl_48Bolidomonas mediterranea MINB11E5

Bolidomonas pacifica OLI31SE3Bolidomonas pacifica OLI51103Bolidomonas pacifica var. eleuthera OLI46SE

Triparma sp. TOY-0807Uncultured Bolidomonas clone MALINA_St320

Tenuicylindrus belgicus DN97S3Leptocylindrus hargravesii CCMP1856

Leptocylindrus danicusLeptocylindrus aporus p372

Leptocylindrus minimus BB951Leptocylindrus convexus B768

Hyalodiscus sp.Hyalodiscus sp. CCMP1679

Paralia sulcataStephanopyxis nipponicaStephanopyxis palmeriana CCMP814

Melosira sp.Melosira cf. octogona CCMP483

Melosira varians X85402Melosira varians CHMP7Melosira varians AJ243065Melosira varians MV13

Aulacoseira crenulataFDCCL639Aulacoseira distansAulacoseira baicalensis

Aulacoseira islandicaCHMP1Aulacoseira subarcticaWLB3

Aulacoseira ambiguaAulacoseira alpigenaCCAP1002/6Proboscia indica CCMP1896

Proboscia alata KTActinoptychus sinensis p394

Actinocyclus actinochilusCCMP107Aulacodiscus sp.

Stellarima microtrias CCMP1471Stellarima microtrias AnM0003

Coscinodiscus radiatusCoscinodiscus granii

Coscinodiscus sp. GGM-2004Rhizosolenia pungens

Rhizosolenia similoidesRhizosolenia setigera

Guinardia flaccida p788Guinardia delicatula p146

Rhizosolenia shubsoleiRhizosolenia fallaxCorethron pennatumCorethron hystrix

Corethron pennatum L29-A3bigCorethron pennatum L29-B2 pollutionCorethron inerme p534Corethron inerme L8-A1Corethron inerme L5-B5Corethron inerme L7-C4Corethron inerme L6-B5Corethron inerme L8-A4

Lampriscus kittoniiToxarium undulatumToxarium hennedyanum ECT3648

Ardissonea baculusClimacosphenia moniligera s0253

Calyptrella robustaBiddulphiopsis titiana

Trigonium formosumTerpsinoe musica

Hydrosera sp.Hemiaulus sinensis CCH-2

Acanthoceras sp. 6vii09-1AUrosolenia eriensis Y98-8Urosolenia eriensis Y98-8

Guinardia sp uncultured ECS_Sp10Eucampia antarctica

Eucampia zoodiacus CCMP386Chaetoceros muelleriiChaetoceros muellerii2

Chaetoceros didymusPhaeoceros sp.

Chaetoceros peruvianusChaetoceros rostratus

Chaetoceros calcitrans f. pumilusCCMP1315Chaetoceros radicans CCMP197

Chaetoceros socialisChaetoceros sp2

Chaetoceros neogracile ArM0004Chaetoceros sp.

Chaetoceros muelleriCCMP1316Chaetoceros gracilisChaetoceros debilis Ch4Chaetoceros curvisetis ch5

Brockmanniella brockmannii CCMP151Papiliocellulus elegans

Minutocellus polymorphus CCMP497Minutocellus sp. RCC967

Leyanella arenariaExtubocellulus cribriger

Cymatosira belgicaOdontella sinensis

Pleurosira laevisOdontella aurita CCAP1054.1

Lithodesmium undulatumDitylum brightwelliiHelicotheca tamesisBellerochea malleus

Porosira pseudodenticulataLauderia annulata

P. pseudodenticulataThalassiosira gessneri ANO2-08

Thalassiosira weissflogii CCMP1010Thalassiosira pseudonana CCMP1057Detonula confervacea

Cyclotella menegheniana WC03-01Cyclotella striata CCMP1586Skeletonema menzelii B82Skeletonema grevilleii CCMP1685Skeletonema dohrnii B104

Skeletonema costatum B211Skeletonema grethae CCMP780Skeletonema pseudocostatumSkeletonema tropicum CCMP2070

Thalassiosira oceanica CCMP1001Minidiscus trioculatus CCMP495Discostella pseudostelligera ROR01-1Cyclostephanos bodanica J98-1

Stephanodiscus hantzschii WTC21Stephanodiscus niagarae OKA-AThalassiosira anguste_lineata MHta1Planktoniella sol p354Detonula pumila NB48Thalassiosira tumida CCMP1469Shionodiscus ritscheri LC01-12Bacterosira bathyomphala NB04-B6

Thalassiosira eccentricaThalassiosira rotula

Attheya cf. septentrionalis strain CCMP2084Attheya longicornis CCMP214

Podocystis spathulata ECT3733Delphineis sp. CCMP1095

Rhaphoneis cf. belgicaRhaphoneis amphiceros

Asteroplanus karyanaAsterionellopsis glacialisAsterionellopsis glacialis WK20

Plagiogramma atomus s0330Neofragilaria nicobarica s0371

Talaroneis posidoniae WK59AB430598.1.1862_U Dimeregramma;Dimeregramma_minor_var._nanumPsammoneis senegalensis s0387Psammoneis japonica s0305Psammoneis pseudojaponica s0354

Araphid pennate sp1.Araphid pennate sp2.

Synedropsis cf.recta CCMP1620Fragilaria barbararum ROSD99Grammonema striatula CCAP1029.18Grammonema islandicaGrammonema striatula CCMP1094

Synedropsis minuscula CCMP845Synedropsis hyperborea CCMP1423Synedropsis fragilaroides CCMP844Grammonema sp.

Hyalosynedra cf.laevigata WK52Catacombas gaillonii WK37

Tabularia fasciculata L778Tabularia tabulata CCMP846Synedra toxoneides WK57

Thalassionema bacillare WK54Thalassionema nitzschioidesThalassionema frauenfeldii WK3Thalassionema frauenfeldii WK5

Synedra marineSynedra sp. p517

Ulnaria ulna WK36Synedra acus At182gel3Synedra angustissima D-16

Fragilaria vaucheriae L1496Fragilaria crotonensisFragilaria rumpens At114Fragilaria capucina D113Fragilariforma virescens V18Fragilaria bidens At170.4

Fragilaria delicatissima At135.13Hyalosira sp. WK50Hyalosira sp. CCMP469Hyalosira delicatula WK51

Grammatophora oceanicaGrammatophora oceanica WK72Grammatophora gibberula WK48Grammatophora marina WK43Grammatophora oceanica CCMP410

Rhabdonema arcuatumLicmophora reichardtii WK63

Licmophora grandis WK62DN82S1 CCMP470 pennate deepak

Synedra berolinensis L1722Staurosira construens

Staurosirella pinnata D91Staurosira elliptica L948

Nanofrustulum shiloi CCMP1306Pseudostaurosira zeilleri v.elliptica D48

Pseudostaurosiropsis sp. I-01Diatoma hyemalis

Asterionella formosaTabellaria flocculosa L1839Diatoma vulgare L1535Diatoma tenue L1251Diatoma tenue v.elongatum L1346Staurosira subsalinaCyclophora tenuisLicmophora juergensii P367

Licmophora flucticulataLicmophora abbreviata

Licmophora gracilisConvoluta endosymbiont

Licmophora communisLicmophora flabellata WK58Licmophora flabellata WK47Licmophora flabellata

Pseudostriatella pacifica s0384Striatella unipunctata

Protoraphis atlantica WK42Pseudohimantidium pacificum

Amphora laevis v.laevissimaGU385679.1.1033_U Bacillariophyta_XXX_sp.

Eunotia monodon v.asiaticaEunotia pectinalisEunotia formica v.sumatrana

Eunophora oberonicaActinella pulchella

Actinella parvaUndatella sp.

AY180015.1.1715_U Bacillariophyta_XXX_sp.Gyrosigma limosum C092Pleurosigma planktonicum C090

Pleurosigma intermedium C089Pleurosigma sp.

Haslea nipkowii C083Haslea crucigera C082Haslea ostrearia C079Haslea pseudostrearia C081

GU385626.1.1698_U Bacillariophyta_XXX_sp.GU385631.1.1691_U Bacillariophyta_XXX_sp.



GU385674.1.1694_U Bacillariophyta_XXX_sp.Navicula lanceolata C086

Navicula arenaria v.rostellataNavicula perminuta

Navicula gregaria AT-117Gel05Navicula sclesviscensis C085

Navicula cryptocephala v.venetaPseudogomphonema sp.

Navicula phyllepta HPSeminavis robusta

Navicula salinarumHQ912404.1.1728_U Hantzschia;Hantzschia_amphioxys

JN033248.1.1192_U Cymbella;Cymbella_cistuliformisPseudo-nitzschia pungens

Pseudo-nitzschia multiseriesGU823436.1.1207_U Bacillariophyta_XXX_sp.

Fragilariopsis cylindricus CCMP1102Fragilariopsis sublinearis

JF791075.1.1777_U Nitzschia;Nitzschia_apiculataAchnanthes bongrainii

Achnanthidium cf. longipesAchnanthes brevipes CCMP100Psammodictyon pandurifore UTCC162

Bacillaria paxilliferTryblionella constrictaNitzschia cf. frustulum

HM799827.1.1722_U Bacillariophyta_XXX_sp.Durinskia baltica endosymbiont

Nitzschia thermalis HPKryptoperidinium foliaceum endosymbiont

FN598376.1.1059_U Bacillariophyta_XXX_sp.Cylindrotheca fusiformis CCMP339

EU333092.1.800_U Bacillariophyta_XXX_sp.Cylindrotheca closterium

Cylindrotheca closterium2FN690543.1.842_U Bacillariophyta_XXX_sp.

HQ912597.1.1724_U Diploneis;Diploneis_subovalisGU823641.1.1061_U Bacillariophyta_XXX_sp.


HQ680564.1.876_U Cymbella;Cymbella_pelagicaHQ680563.1.876_U Cymbella;Cymbella_parva

HQ912644.1.1591_U Climaconeis;Climaconeis_riddleaeLyrella atlantica

HQ912413.1.1746_U Campylodiscus;Campylodiscus_sp.HQ912609.1.1719_U Scoliopleura;Scoliopleura_peisonis

HQ912591.1.1742_U Neidium;Neidium_bisulcatumHQ912583.1.1742_U Neidium;Neidium_affine

HQ912582.1.1733_U Neidium;Neidium_productumAM501955.1.1727_U Neidium;Neidium_affine

Anomoeoneis sphaerophora L1222Encyonema angulatum L1313

Gomphonema parvulumGomphonema gracile

AM502016.1.1724_U Cymbella;Cymbella_asperaHQ655885.1.1724_U Reimeria;Reimeria_sinuataHQ655880.1.1724_U Cymbella;Cymbella_navicula

Phaeodactylum tricornutumCocconeis placentula AT-212Gel11

HQ912626.1.1750_U Lemnicola;Lemnicola_hungaricaPauliella taeniata CCMP1115

Dickieia ulvaceaPlanothidium lanceolatum L1249

Diadesmis gallicaDQ310321.1.1054_U Bacillariophyta_XXX_sp.

HQ912637.1.1736_U Berkeleya;Berkeleya_rutilansDQ310229.1.1384_U Bacillariophyta_XXX_sp.DQ310238.1.1381_U Bacillariophyta_XXX_sp.Biremis ambigua

Brachysira cf. styriacaParlibellus cf.plicatus

Navicula pelliculosa CCMP543Eolimna subminuscula

Pinnularia microstauron AT-69.06Pinnularia acrosphaeria AT-161.03

Caloneis amphisbaena AT-177.07Pinnunavis cf.elegans

Fallacia forcipataHQ912605.1.1745_U Fallacia;Fallacia_pygmaea

Amphora coffeaeformis CCAP1008/1Eolimna minimaSellaphora auldreekie DUN1

Sellaphora pupula GER1Amphora lybica AT-117.10Amphora cf. proteus p386Amphora.montana

Amphora cf. capitellata p363Amphora cf. coffeaeformis CCAP1008/1

HQ912415.1.1727_U Surirella;Surirella_splendidaStenopterobia cf.curvataHQ912416.1.1725_U Stenopterobia;Stenopterobia_curvula

HQ912414.1.1752_U Surirella;Surirella_sp.Amphiproa paludosa CCMP125

Amphiprora alata CCAP1003/3Entomoneis cf. alata CCMP1522

Surirella sp.HQ912658.1.1741_U Surirella;Surirella_minuta

Surirella angusta SANG1Surirella brebissoni SBRE3

0.01 substitutions/site

NJ

Heterotrophic Stramenopiles

“other” autotrophic Stramenopiles

Bolidomonads and Parmales

Other Radial Centrics

Bi- and Multipolar Centrics

Pennates

T. belgicus L. hargravesii

L. danicus L. aporus

L. minimus L. convexus


2µm

Triangular “wings”

Annulus

Subcentral pore

Striae

Valve Valve face

Valve mantle

Chain of cells

Butterfly shaped cingular band

Leptocylindrus


Tenuicylindrus

Scale Bar: A–B 5µm C–J 1µm


Basilicostephanus

Harwood and Gersonde, 1990 Gersonde and Harwood, 1990

Gladiopsis

Kerkis

Rhynchopyxis


Scale bars 1µm

Leptocylindrus danicus

Leptocylindrus danicus, L. hargravesii and L minimus form resting spores immediately upon sexual reproduction.


L. aporus L. danicus L. dan

L. convexus

L.h L.h

Diversity and abundance of Leptocylindrus spp. in the plankton of the Gulf of Naples


Diversity and abundance of Tenuicylindrus belgicus in the plankton of the Gulf of Naples

Can we use BioMarKs V4 sequence data gathered all across European coasts to assess the biodiversity of these genera?

Two questions: •  Can we find the same species in other places?

•  Can we find yet undiscovered diversity within the two genera?




–  Yes! Based on highly similar or identical V4-sequences



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Naples 2009 Naples 2010 Oslo 2009 Oslo 2010 Barcelona 2010 Gijon 2010

Leptocylindrus minimus Leptocylindrus danicus Tenuicylindrus belgicus Leptocylindrus convexus Leptocylindrus aporus

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Leptocylindrus minimus Leptocylindrus danicus Tenuicylindrus belgicus Leptocylindrus convexus Leptocylindrus aporus1

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Leptocylindrus dancius/hargravesii

Leptocylindrus minimus

Tenuicylindrus belgicus

Leptocylindrus convexus

Leptocylindrus aporus



•  Can we find yet undiscovered diversity within the two genera? –  Yeah, but with some trouble!


The V4-tree

Pirsonia punctigeraHyphochytrium catenoidesDevelopayella elegans

Achlya apiculataPhytophthora undulata

Fibrocapsa japonica CCMP1661Chattonella antiqua

Haramonas viridisPolypodochrysis teissieri

Pinguiochrysis pyriformisGlossomastix chrysoplasta

Dictyocha fibulaSynchroma grandeSarcinochrysis marina

Aureococcus anophagefferensPelagomonas calceolata

Florenciella parvulaVerrucophora farcimen

Dictyocha speculumRhizochromulina marina

Helicopedinella tricostataPteridomonas danica

Pseudotetraedriella kamillaeVischeria helveticaEustigmatos magna

Ochromonas danicaChrysolepidomonas dendrolepidota

Chromulina chionophilaChrysosphaera parvula

Mallomonas adamasOchromonas tuberculata

Phaeoplaca thallosaTessellaria volvocina CCMP1781

Mallomonas annulataSynura curtispina CCMP847

Tetrasporopsis fuscescensGiraudyopsis stelliferaAntarctosaccion applanatum

Pleurochloridella botrydiopsisStichogloea doederleinii

Botrydiopsis alpinaBotrydiopsis constricta

Heterococcus pleurococcoidesMischococcus sphaerocephalus

Phaeothamnion confervicolaChoristocarpus tenellus

Discosporangium mesarthrocarpumDictyota dichotomaBodanella lauterborni

Spatoglossum pacificumSporochnus comosusSaccharina japonica

Bolidomonas mediterranea MINB11E5marine eukaryote NW414.14

Uncultured marine diatom DH22_2A36Uncultured eukaryote DSGM-35

Uncultured stramenopile 1-E8Uncultured eukaryote A10

Uncultured stramenopile 3b-A8freshwater eukaryote LG22-09

Uncultured stramenopile Bothnian BayMarine eukaryote BLACKSEA_cl_48

Bolidomonas pacifica OLI31SE3Bolidomonas pacifica OLI51103

Bolidomonas pacifica var. eleuthera OLI46SETriparma sp. TOY-0807Uncultured Bolidomonas clone MALINA_St320

Tenuicylindrus belgicus DN97S3Porosira pseudodenticulata

Lauderia annulataLeptocylindrus hargravesii CCMP1856Leptocylindrus danicus

Leptocylindrus aporus p372Leptocylindrus minimus BB951

Leptocylindrus convexus B768Aulacodiscus sp.

Actinoptychus sinensis p394Actinocyclus actinochilusCCMP107Chrysochaete britannica

Chromophyton rosanoffiiChrysocapsa paludosa

Chrysonebula flavaHibberdia magna

HQ680564.1.876_U Cymbella;Cymbella_pelagicaHQ680563.1.876_U Cymbella;Cymbella_parva

Melosira sp.Melosira cf. octogona CCMP483

Melosira varians X85402Melosira varians CHMP7Melosira varians AJ243065Melosira varians MV13

Terpsinoe musicaHyalodiscus sp. CCMP1679

Hyalodiscus sp.Rhizosolenia pungensRhizosolenia similoides

Corethron pennatumCorethron hystrix

Corethron pennatum L29-A3bigCorethron pennatum L29-B2 pollutionCorethron inerme p534Corethron inerme L5-B5

Corethron inerme L8-A4Corethron inerme L8-A1Corethron inerme L7-C4Corethron inerme L6-B5

Lampriscus kittoniiToxarium undulatum

Toxarium hennedyanum ECT3648Ardissonea baculusClimacosphenia moniligera s0253

Hydrosera sp.Acanthoceras sp. 6vii09-1AUrosolenia eriensis Y98-8

Urosolenia eriensis Y98-8Hemiaulus sinensis CCH-2

Guinardia sp uncultured ECS_Sp10Lithodesmium undulatum

Bellerochea malleusHelicotheca tamesisDitylum brightwellii

Chaetoceros didymusPhaeoceros sp.Chaetoceros peruvianus

Chaetoceros rostratusChaetoceros muellerii

Chaetoceros muellerii2Chaetoceros calcitrans f. pumilusCCMP1315

Chaetoceros radicans CCMP197Chaetoceros socialis

Chaetoceros sp2Chaetoceros neogracile ArM0004Chaetoceros sp.

Chaetoceros muelleriCCMP1316Chaetoceros gracilisChaetoceros debilis Ch4Chaetoceros curvisetis ch5

Attheya cf. septentrionalis strain CCMP2084Attheya longicornis CCMP214Paralia sulcata

Stephanopyxis nipponicaStephanopyxis palmeriana CCMP814

Thalassiosira gessneri ANO2-08Thalassiosira weissflogii CCMP1010

Thalassiosira pseudonana CCMP1057Detonula confervaceaCyclotella menegheniana WC03-01Cyclotella striata CCMP1586Skeletonema menzelii B82

Skeletonema grethae CCMP780Skeletonema grevilleii CCMP1685Skeletonema costatum B211Skeletonema dohrnii B104

Skeletonema pseudocostatumSkeletonema tropicum CCMP2070

Discostella pseudostelligera ROR01-1Cyclostephanos bodanica J98-1

Stephanodiscus hantzschii WTC21Stephanodiscus niagarae OKA-ABacterosira bathyomphala NB04-B6

Thalassiosira eccentricaThalassiosira rotulaThalassiosira anguste_lineata MHta1Detonula pumila NB48

Planktoniella sol p354Thalassiosira tumida CCMP1469

Minidiscus trioculatus CCMP495Thalassiosira oceanica CCMP1001Shionodiscus ritscheri LC01-12

Stellarima microtrias CCMP1471Stellarima microtrias AnM0003Trigonium formosum

Eucampia antarcticaEucampia zoodiacus CCMP386

Calyptrella robustaCoscinodiscus radiatus

Coscinodiscus graniiCoscinodiscus sp. GGM-2004

Proboscia indica CCMP1896Proboscia alata KT

Rhizosolenia setigeraGuinardia flaccida p788

Pleurosira laevisOdontella aurita CCAP1054.1

Guinardia delicatula p146Rhizosolenia shubsolei

Rhizosolenia fallaxBiddulphiopsis titiana

Odontella sinensisP. pseudodenticulata

Brockmanniella brockmannii CCMP151Leyanella arenaria

Extubocellulus cribrigerCymatosira belgica

Papiliocellulus elegansMinutocellus polymorphus CCMP497Minutocellus sp. RCC967

GU385679.1.1033_U Bacillariophyta_XXX_sp.Aulacoseira crenulataFDCCL639

Aulacoseira distansAulacoseira ambiguaAulacoseira subarcticaWLB3Aulacoseira alpigenaCCAP1002/6Aulacoseira baicalensisAulacoseira islandicaCHMP1

Podocystis spathulata ECT3733HQ912644.1.1591_U Climaconeis;Climaconeis_riddleaeGomphonema parvulum

Gomphonema gracileAnomoeoneis sphaerophora L1222

Pinnularia acrosphaeria AT-161.03HQ912413.1.1746_U Campylodiscus;Campylodiscus_sp.

Lyrella atlanticaAM502016.1.1724_U Cymbella;Cymbella_asperaHQ655885.1.1724_U Reimeria;Reimeria_sinuataHQ655880.1.1724_U Cymbella;Cymbella_navicula

HQ912609.1.1719_U Scoliopleura;Scoliopleura_peisonisHQ912591.1.1742_U Neidium;Neidium_bisulcatumHQ912583.1.1742_U Neidium;Neidium_affine

HQ912582.1.1733_U Neidium;Neidium_productumAM501955.1.1727_U Neidium;Neidium_affine

Undatella sp.Haslea nipkowii C083

Haslea crucigera C082Haslea ostrearia C079Haslea pseudostrearia C081

AY180015.1.1715_U Bacillariophyta_XXX_sp.Gyrosigma limosum C092

Pleurosigma planktonicum C090Pleurosigma intermedium C089Pleurosigma sp.

Araphid pennate sp1.Pseudogomphonema sp.

Navicula phyllepta HPNavicula gregaria AT-117Gel05Navicula sclesviscensis C085

GU385674.1.1694_U Bacillariophyta_XXX_sp.Navicula lanceolata C086Navicula arenaria v.rostellataNavicula perminuta

Navicula cryptocephala v.venetaSeminavis robustaNavicula salinarum

GU823641.1.1061_U Bacillariophyta_XXX_sp.Araphid pennate sp2.

HQ912637.1.1736_U Berkeleya;Berkeleya_rutilansDQ310238.1.1381_U Bacillariophyta_XXX_sp.

DQ310229.1.1384_U Bacillariophyta_XXX_sp.GU385690.1.1340_U Bacillariophyta_XXX_sp.

Cocconeis placentula AT-212Gel11Encyonema angulatum L1313

GU385626.1.1698_U Bacillariophyta_XXX_sp.Planothidium lanceolatum L1249

HQ912626.1.1750_U Lemnicola;Lemnicola_hungaricaPauliella taeniata CCMP1115Dickieia ulvacea

HQ912597.1.1724_U Diploneis;Diploneis_subovalisDiadesmis gallica

Amphora coffeaeformis CCAP1008/1Eolimna minimaSellaphora auldreekie DUN1

Sellaphora pupula GER1Fallacia forcipata

HQ912605.1.1745_U Fallacia;Fallacia_pygmaeaBiremis ambigua

Pinnularia microstauron AT-69.06Caloneis amphisbaena AT-177.07Pinnunavis cf.elegans

Amphora cf. coffeaeformis CCAP1008/1Amphora.montana

Amphora cf. capitellata p363Amphora lybica AT-117.10

Amphora cf. proteus p386Parlibellus cf.plicatus

Navicula pelliculosa CCMP543Eolimna subminuscula

HQ912415.1.1727_U Surirella;Surirella_splendidaStenopterobia cf.curvata

HQ912416.1.1725_U Stenopterobia;Stenopterobia_curvulaHQ912414.1.1752_U Surirella;Surirella_sp.

Amphiproa paludosa CCMP125Amphiprora alata CCAP1003/3

Entomoneis cf. alata CCMP1522Surirella sp.

HQ912658.1.1741_U Surirella;Surirella_minutaSurirella angusta SANG1Surirella brebissoni SBRE3

HQ912404.1.1728_U Hantzschia;Hantzschia_amphioxysJN033248.1.1192_U Cymbella;Cymbella_cistuliformis

GU823436.1.1207_U Bacillariophyta_XXX_sp.Fragilariopsis cylindricus CCMP1102Fragilariopsis sublinearisPseudo-nitzschia pungens

Pseudo-nitzschia multiseriesFN598376.1.1059_U Bacillariophyta_XXX_sp.GU385662.1.1670_U Bacillariophyta_XXX_sp.

GU385685.1.1700_U Bacillariophyta_XXX_sp.Eunotia monodon v.asiaticaEunotia pectinalis

Eunotia formica v.sumatranaEunophora oberonica

Actinella pulchellaActinella parvaCylindrotheca closteriumCylindrotheca closterium2

Cylindrotheca fusiformis CCMP339FN690543.1.842_U Bacillariophyta_XXX_sp.

EU333092.1.800_U Bacillariophyta_XXX_sp.Amphora laevis v.laevissimaAchnanthes bongrainii

Psammodictyon pandurifore UTCC162GU385639.1.1696_U Bacillariophyta_XXX_sp.

Bacillaria paxilliferAchnanthidium cf. longipes

Achnanthes brevipes CCMP100Tryblionella constricta

Kryptoperidinium foliaceum endosymbiontNitzschia cf. frustulumDurinskia baltica endosymbiontGU385631.1.1691_U Bacillariophyta_XXX_sp.

GU385609.1.1711_U Bacillariophyta_XXX_sp.Nitzschia thermalis HP

GU822956.1.1203_U Bacillariophyta_XXX_sp.HM799827.1.1722_U Bacillariophyta_XXX_sp.

Phaeodactylum tricornutumDQ310321.1.1054_U Bacillariophyta_XXX_sp.

JF791075.1.1777_U Nitzschia;Nitzschia_apiculataHyalosira sp. WK50Hyalosira sp. CCMP469Hyalosira delicatula WK51

Brachysira cf. styriacaProtoraphis atlantica WK42

Pseudohimantidium pacificumSynedra marine

Synedra sp. p517Ulnaria ulna WK36Synedra acus At182gel3Synedra angustissima D-16

Thalassionema nitzschioidesThalassionema bacillare WK54Thalassionema frauenfeldii WK3

Thalassionema frauenfeldii WK5Hyalosynedra cf.laevigata WK52

Synedra toxoneides WK57Catacombas gaillonii WK37

Tabularia fasciculata L778Tabularia tabulata CCMP846

Fragilaria vaucheriae L1496Fragilaria crotonensisFragilaria rumpens At114Fragilaria capucina D113

Fragilariforma virescens V18Fragilaria bidens At170.4Fragilaria delicatissima At135.13

Pseudostriatella pacifica s0384Striatella unipunctata

Licmophora flucticulataLicmophora abbreviata

Licmophora gracilisLicmophora grandis WK62Convoluta endosymbiontLicmophora communis

Rhabdonema arcuatumGrammonema striatula CCAP1029.18

Grammonema islandicaSynedropsis cf.recta CCMP1620Grammonema striatula CCMP1094Fragilaria barbararum ROSD99Synedropsis minuscula CCMP845Synedropsis hyperborea CCMP1423Synedropsis fragilaroides CCMP844Grammonema sp.

Grammatophora oceanicaGrammatophora oceanica CCMP410Grammatophora oceanica WK72Grammatophora marina WK43Grammatophora gibberula WK48

Cyclophora tenuisLicmophora reichardtii WK63

DN82S1 CCMP470 pennate deepakLicmophora juergensii P367Licmophora flabellataLicmophora flabellata WK47Licmophora flabellata WK58

Asteroplanus karyanaAsterionellopsis glacialisAsterionellopsis glacialis WK20

Delphineis sp. CCMP1095Rhaphoneis cf. belgica

Rhaphoneis amphicerosStaurosirella pinnata D91

Staurosira construensSynedra berolinensis L1722Staurosira elliptica L948Nanofrustulum shiloi CCMP1306

Pseudostaurosira zeilleri v.elliptica D48Pseudostaurosiropsis sp. I-01

AB430598.1.1862_U Dimeregramma;Dimeregramma_minor_var._nanumTalaroneis posidoniae WK59

Plagiogramma atomus s0330Neofragilaria nicobarica s0371Psammoneis japonica s0305Psammoneis pseudojaponica s0354Psammoneis senegalensis s0387

Asterionella formosaDiatoma hyemalis

Diatoma vulgare L1535Tabellaria flocculosa L1839Staurosira subsalinaDiatoma tenue L1251Diatoma tenue v.elongatum L1346

0.01 substitutions/site

NJ

L. hargravesii L. danicus

L. aporus L. minimus

L. convexus


Heterotrophic Stramenopiles

“most” autotrophic Stramenopiles

Bolidomonads and Parmales

Other Radial Centrics and a few Stramenopiles

Bi-Multipolar, and a few radial Centrics

Pennates

Porosira & Lauderia T. belgicus

10% dissimilarity

Ldan_382b87d93ce960f24faeee43512a1758_1 Lharg dcefcbb168cf68d9d61b94967207ed0b_1 Lharg 81e5d1a40e3b1f2e6e76ff87a5fad302_2 Ldan_49de97debf5a4bdf34a02e745eea4331_1 Lharg_2807c3131b3356d4d03c8a015bf1e64b_1 Lharg 5d681a0f9e2f894241f70363d81e50b1_1

Lharg 847f436624de6a861121d8fdcbcbd035_1 Lharg_294c718549e6d44740195c3020cbbdad_2 Ldan_403d4e8bef56dc568bb0dd04619a6709_2

Lharg 48a4f876508ee8b7e69fe60741c33e6b_1 Ldan_fb349487abc6abf2bfe46e42c95db8ca_1

Lharg_2a68188090520caa8d81c45a0a443188_4 GU824887

Lharg_ff503d2534c999af974bf427c46df44e_8 Leptocylindrus hargravesii Lharg Leptocylindrus hargravesii_635 GU825201

GU824332 Lharg_76607d003c01e727ee17b4630cbf842a_1

GU825183 Leptocylindrus danicus Ldan Leptocylindrus danicus_68

GU824143 Leptocylindrus convexus_17 Leptocylindrus convexus GU823919

GU825668 Leptocylindrus minimus NCBI

Lmini_22170c8c5b9a7a1daf53f4d7066987f0_185 Lcon_355d80c0a1f0b317384480a48cae4cb0_42

Lmini 42da7abc101a949c189137e89ca987ab_16 Lmini 64e6ca789c83f3a1258cc18cd8defb78_3

Lapo_e9dede35a7076885146edd819db18e25_1 Naples2009 Lapo_a88ade6550b62188339dbf44ab3e56c3_24

Lapo_12388426d16bccda4ab619cd18fe6792_1 Lapo_Leptocylindrus aporus_48820

Lapo_e93487fb115d07d82421cc106f2a9283_1 Lapo_442fa2332449e0593cd2d47b6f3ed7f6_5

Lapo_8b3d183186a056081e73ef80cf8c53fe_3 Lapo_bab055b2f4b42689a9490af9e726fed3_20

Lapo_987d26ffc34dbf1088def1f5e9cc6190_1 Lapo_c90e2b3ba682d9e90c980689ae962986_1

Tbel d879885476c520772996a2a230446060_1 Tbel 6755f0b4633e96ebb8ea275997fd3935_1

Tbel 0b38196304d4cf65273e7800b2b1494a_1 Tbel c20e86a160753ce12126e809c385537a_1

Tbel 37ae65a8517aa36e460bf8812a378802_1 Tbel c9b3c284663d66bd56fed02b5cec97dc_1

Tbel bc86d8d21a404eabe412968d81b0ab85_1 Tenuicylindrus belgicus Tbel_0577c4678c8eec0a9ad9693527326f03_165

Other Centrics

L. hargravesii and L. danicus

L. aporus

L. minimus

L. convexus

T. belgicus

L. something new?

Bolidomonas

10% dissimilarity

diversity of - biomarksbiomarks.eu/sites/default/files/wiebe-light.pdf · 2014-10-29 · diversity...

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